scholarly journals Morphofunctional analysis of antigen uptake mechanisms following sublingual immunotherapy with beads in mice

2018 ◽  
Author(s):  
Yaser Hosny Ali Elewa ◽  
Tatsuya Mizoguchi ◽  
Osamu Ichii ◽  
Teppei Nakamura ◽  
Yasuhiro Kon
Keyword(s):  
Fluorescence Intensity ◽  
Sublingual Immunotherapy ◽  
Local Immunity ◽  
Antigen Uptake ◽  
Age Related ◽  
Silica Beads ◽  
Light Fluorescence ◽  
Fluorescence Stereomicroscope ◽  
Sublingual Mucosa ◽  
Uptake Mechanisms

AbstractBackgroundRecently, sublingual immunotherapy (SLIT) has been used as a safe and efficient method for the treatment of and immunization against asthma and various allergies. However, the routes of antigen uptake through the mucosa of the oral cavity remain incompletely understood, as do the roles of sex and age in the process. For this purpose, to elucidate the mechanism and efficacy of SLIT among different sexes and ages microbeads were dripped into the sublingual region to mimic antigen uptake by the sublingual mucosa.MethodsTwenty microliters of either phosphate buffered saline (PBS) or fluorescently labelled microbeads (latex and silica beads) were placed under the tongue of both male and female C57BL/6 mice at young (3 months) and old (6 months) ages. The lower jaw was examined 30 min after administration, and beads were detected with a fluorescence stereomicroscope. Morphological observations of the mucosa of the fluorescent areas were made with scanning electron microscopy (SEM) and an all-in-one light fluorescence microscope (LM). Fluorescence intensity was compared between both sexes and ages.ResultsStereomicroscopic observation revealed fluorescent illuminations in three compartments of the sublingual mucosa: the sublingual caruncles (SC), the oral rostral mucosa (OR) and the buccal mucosa (BM). Interestingly, the fluorescence intensity tended to be higher among females than among males in the SC region in particular. However, there were no significant age-related differences. SEM and LM revealed beads in the lumina of both mandibular ducts and sublingual ducts (Sd). Additionally, the apical cytoplasm of some Sd cells contained silica beads. However, there were no specification in the OR mucosa or BM.ConclusionsThis study reveals the major role Sd play in local immunity via the antigen uptake mechanisms. Furthermore, our data suggest that the efficacy of SLIT in humans could be affected by sex.

scholarly journals Morphofunctional analysis of antigen uptake mechanisms following sublingual immunotherapy with beads in mice

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0201330
Author(s):  
Yaser Hosny Ali Elewa ◽  
Tatsuya Mizoguchi ◽  
Osamu Ichii ◽  
Teppei Nakamura ◽  
Yasuhiro Kon
Keyword(s):  
Sublingual Immunotherapy ◽  
Antigen Uptake ◽  
Morphofunctional Analysis ◽  
Uptake Mechanisms

scholarly journals Endometrial Infertility in Patients of Late Reproductive age (a review)

2020 ◽  
Vol 27 (6) ◽  
pp. 149-163
Author(s):  
K. V. Uryupina ◽  
I. I. Kutsenko ◽  
E. I. Kravtsova ◽  
J. V. Kudlai ◽  
I. I. Kravtsov
Keyword(s):  
Assisted Reproduction ◽  
Endometrial Thickness ◽  
Uterine Cavity ◽  
Endometrial Receptivity ◽  
Female Infertility ◽  
Reproductive Age ◽  
Local Immunity ◽  
Age Related ◽  
Choice Of Treatment ◽  
Uterine Infertility

Background. Endometrial infertility is a frequent cause of failure in assisted reproduction. Causes of endometrial infertility are manifold and require comprehensive assessment for a successful choice of treatment strategy.Objectives. A review of infertility concepts accounting for endometrial infertility in women of late reproductive age.Methods. Bibliographic analysis: sources for review were mined in the PubMed, MedLine, eLibrary and Cyberleninka databases at a depth of 10 years. Keyword queries were: endometrial factors of infertility, uterine infertility [маточные факторы бесплодия], causes of infertility. Selected articles related to female infertility and, particularly, endometrial factors of infertility. Low-informative articles were not considered.Results. A total of 51 sources were analysed, with 36 selected in the review. The reviewed evidence suggests that endometrial female infertility in late reproductive age is associated with cumulative gynaecological pathology and age-related change adversely impacting endometrial receptivity and synchrony with embryo maturation in assisted reproductive protocols.Conclusion. Determining the functional status of endometrium is prerequisite for the outcome prognosis in assisted reproduction due to feasible failures to conceive with a vital embryo but reduced endometrial receptivity. This observation warrants a timely diagnosis and treatment of endometrial disorders prior to having assisted reproductive interventions. Woman’s age is the main predictor of successful pregnancy in IVF/ICSI protocols. Among the main markers of successful implantation is endometrial thickness. Uterine infertility may relate to impaired local immunity and autoimmune responses in uterine cavity. The most common mechanisms of uterine infertility are associated uterine myoma, endometriosis and endometritis. Women with uterine infertility attempting IVF/ICSI procedures often exhibit asynchronous endometrial development relative to the embryo maturity for implantation.

Age-Related Changes in the Human Laryngeal Glands

1998 ◽  
Vol 107 (6) ◽  
pp. 525-529 ◽  
Author(s):  
Kiminori Sato ◽  
Minoru Hirano
Keyword(s):  
Secretory Granules ◽  
Vocal Folds ◽  
Mucous Cells ◽  
Electron Microscopic ◽  
Local Immunity ◽  
Laryngeal Function ◽  
Age Related ◽  
Transmission Electron ◽  
Age Related Changes

The present study was conducted to determine age-related changes in the human laryngeal glands by means of excised human adult larynges. Scanning and transmission electron microscopic observations were made. The results obtained are summarized as follows. 1) Granular endoplasmic reticulum and Golgi apparatus were sparse in the cytoplasm of serous and mucous cells. 2) Secretory granules in serous cells had decreased in number. 3) Secretory granules were less electron-dense compared to those in younger adult specimens, but were electron-lucent. 4) Mucigen droplets in mucous cells were not as numerous as those in younger adults. 5) Discharge of secretory granules and mucigen droplets had decreased. 6) Age-related morphologic changes in the laryngeal glands influenced not only the amount but also the quality of secretions. 7) The above changes lessened lubrication of the vocal folds, thus causing aging of the voice to some extent. 8) Local immunity and mucociliary transport were also affected. 9) Age-related changes in the laryngeal glands partially altered laryngeal function.

scholarly journals Use of a FACS III for fluorescence depolarization with DPH.

1989 ◽  
Vol 37 (11) ◽  
pp. 1653-1658 ◽  
Author(s):  
G Böck ◽  
L A Huber ◽  
G Wick ◽  
K N Traill
Keyword(s):  
Steady State ◽  
Fluorescence Intensity ◽  
Human Lymphocyte ◽  
Blood Donors ◽  
Lymphocyte Subsets ◽  
Fluorescence Probe ◽  
Functional Importance ◽  
Fluorescence Depolarization ◽  
Age Related ◽  
Monocytes And Lymphocytes

We have previously demonstrated age-related differences in human lymphocyte membrane fluidity, by use of steady-state polarization measurements on bulk cell suspensions with the fluorescence probe DPH. However, for exact analysis of the possible functional importance of these changes, single-cell measurements were deemed of interest. We have now used an analog division device to measure fluorescence depolarization "p" of DPH in real time with a FACS III flow cytometer. The measurements are reliable, as we have been able to confirm the differences in DPH "p" between monocytes and lymphocytes previously shown in bulk suspension and to demonstrate the expected differences in fluidity of lipid-modulated cells. We also found significant differences in DPH "p" between lymphocytes of young and elderly blood donors. Lymphocyte subsets did not differ in polarization values but did differ in fluorescence intensity with Th less than Ts less than B = NK cells.

scholarly journals Dynamics of clinical symptoms and immunity local parametres during allergen - specific immunotherapy in patents with pollinosis and combined forms of sensibilization

2009 ◽  
Vol 7 (3) ◽  
pp. 14-18
Author(s):  
E V Bezrukova ◽  
B A Molotilov
Keyword(s):  
Czech Republic ◽  
Oral Cavity ◽  
Specific Immunotherapy ◽  
Sublingual Immunotherapy ◽  
Clinical Symptoms ◽  
Pollen Allergens ◽  
Local Immunity ◽  
Allergen Specific Immunotherapy ◽  
Made In

Background. To evaluate clinical and immunological efficiency of immunotherapy of pollinosis patients with combined forms of sensitization with «Sevapharma» allergens, Czech Republic. Materials and methods. 189 patients with pollinosis (age 18-55 years old) were examined during this study. Out of these 189 patients three groups were chosen to be treated with pollen allergens made in Czech Republic and domestic allergens from Russia. During the study slgA in saliva was examined and the efficiency of different AS IT methods with combined forms of sensitization was tested. Results. Patients with pollinosis had disruption in local immunity. slASIT increases slgA production in saliva. Treatment with interfacing allergens is most effective in combined forms of sensitization. Conclusion. The sensibilization to different kinds of pollen allergens was observed in 10- 15% of cases. Combined forms of sensitization are much more common. Moreover, combined forms of sensitization to pollen and domestic allergens predominate. Besides that, the study showed pollinosis patients have disruption of local immunity that appeared in slgA deficit in oral cavity. Sublingual immunotherapy is the most effective method of treatment. In case of combined forms of sensitization treatment with interfacing allergens is the most efficient.

Spindle shaped bodies in foveolar cones of the baboon retina

1989 ◽  
Vol 47 ◽  
pp. 960-961
Author(s):  
W. Krebs ◽  
I. Krebs
Keyword(s):  
Cross Sections ◽  
Inclusion Bodies ◽  
Photoreceptor Cells ◽  
Papio Anubis ◽  
Retinal Photoreceptor ◽  
Age Related ◽  
Cone Cells ◽  
Membrane Bound ◽  
Oriented Parallel ◽  
Shaped Inclusion

Various inclusion bodies occur in vertebrate retinal photoreceptor cells. Most of them are membrane bound and associated with phagocytosis or they are age related residual bodies. We found an additional inclusion body in foveal cone cells of the baboon (Papio anubis) retina.The eyes of a 15 year old baboon were fixed by immersion in cacodylate buffered glutaraldehyde (2%)/formaldehyde (2%) as described in detail elsewhere . Pieces of retina from various locations, including the fovea, were embedded in epoxy resin such that radial or tangential sections could be cut.Spindle shaped inclusion bodies were found in the cytoplasm of only foveal cones. They were abundant in the inner segments, close to the external limiting membrane (Fig. 1). But they also occurred in the outer fibers, the perikarya, and the inner fibers (Henle’s fibers) of the cone cells. The bodies were between 0.5 and 2 μm long. Their central diameter was 0.2 to 0. 3 μm. They always were oriented parallel to the long axis of the cone cells. In longitudinal sections (Figs. 2,3) they seemed to have a fibrous skeleton that, in cross sections, turned out to consist of plate-like (Fig.4) and tubular profiles (Fig. 5).

Morphogenetic machines revealed: Microscopy of living cells in the embryo of the frog, Xenopus laevis

1993 ◽  
Vol 51 ◽  
pp. 172-173
Author(s):  
Ray Keller
Keyword(s):  
Image Processing ◽  
Fluorescent Dyes ◽  
Cell Movement ◽  
Living Cells ◽  
Ease Of Use ◽  
Low Light ◽  
Amphibian Embryo ◽  
Large Populations ◽  
Light Fluorescence ◽  
Video Image Processing

The amphibian embryo offers advantages of size, availability, and ease of use with both microsurgical and molecular methods in the analysis of fundamental developmental and cell biological problems. However, conventional wisdom holds that the opacity of this embryo limits the use of methods in optical microscopy to resolve the cell motility underlying the major shape-generating processes in early development.These difficulties have been circumvented by refining and adapting several methods. First, methods of explanting and culturing tissues were developed that expose the deep, nonepithelial cells, as well as the superficial epithelial cells, to the view of the microscope. Second, low angle epi-illumination with video image processing and recording was used to follow patterns of cell movement in large populations of cells. Lastly, cells were labeled with vital, fluorescent dyes, and their behavior recorded, using low-light, fluorescence microscopy and image processing. Using these methods, the details of the cellular protrusive activity that drives the powerful convergence (narrowing)

Autophagy and ageing: implications for age-related neurodegenerative diseases

2013 ◽  
Vol 55 ◽  
pp. 119-131 ◽  
Author(s):  
Bernadette Carroll ◽  
Graeme Hewitt ◽  
Viktor I. Korolchuk
Keyword(s):  
Neurodegenerative Diseases ◽  
Energy Availability ◽  
Future Studies ◽  
Intracellular Degradation ◽  
Age Related ◽  
Autophagy Induction ◽  
Social Importance ◽  
Cellular Dysfunction ◽  
Autophagy Activity ◽  
Intense Research

Autophagy is a process of lysosome-dependent intracellular degradation that participates in the liberation of resources including amino acids and energy to maintain homoeostasis. Autophagy is particularly important in stress conditions such as nutrient starvation and any perturbation in the ability of the cell to activate or regulate autophagy can lead to cellular dysfunction and disease. An area of intense research interest is the role and indeed the fate of autophagy during cellular and organismal ageing. Age-related disorders are associated with increased cellular stress and assault including DNA damage, reduced energy availability, protein aggregation and accumulation of damaged organelles. A reduction in autophagy activity has been observed in a number of ageing models and its up-regulation via pharmacological and genetic methods can alleviate age-related pathologies. In particular, autophagy induction can enhance clearance of toxic intracellular waste associated with neurodegenerative diseases and has been comprehensively demonstrated to improve lifespan in yeast, worms, flies, rodents and primates. The situation, however, has been complicated by the identification that autophagy up-regulation can also occur during ageing. Indeed, in certain situations, reduced autophagosome induction may actually provide benefits to ageing cells. Future studies will undoubtedly improve our understanding of exactly how the multiple signals that are integrated to control appropriate autophagy activity change during ageing, what affect this has on autophagy and to what extent autophagy contributes to age-associated pathologies. Identification of mechanisms that influence a healthy lifespan is of economic, medical and social importance in our ‘ageing’ world.

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