scholarly journals Fluorescence Multiplexing with Spectral Imaging and Combinatorics

2018 ◽  
Author(s):  
Hadassa Y. Holzapfel ◽  
Alan D. Stern ◽  
Mehdi Bouhaddou ◽  
Catilin M. Anglin ◽  
Danielle Putur ◽  
...  
Keyword(s):  
Emission Spectra ◽  
Spectral Imaging ◽  
Excitation Wavelength ◽  
Spectral Emission ◽  
Experimental Proof ◽  
Measurement Tools ◽  
Wavelength Scanning ◽  
Covalently Linked ◽  
Proof Of Principle

ABSTRACTUltraviolet-to-infrared fluorescence is a versatile and accessible assay modality, but is notoriously hard to multiplex due to overlap of wide emission spectra. We present an approach for fluorescence multiplexing using spectral imaging and combinatorics (MuSIC). MuSIC consists of creating new independent probes from covalently-linked combinations of individual fluorophores, leveraging the wide palette of currently available probes with the mathematical power of combinatorics. Probe levels in a mixture can be inferred from spectral emission scanning data. Theory and simulations suggest MuSIC can increase fluorescence multiplexing ~4-5 fold using currently available dyes and measurement tools. Experimental proof-of-principle demonstrates robust demultiplexing of nine solution-based probes using ~25% of the available excitation wavelength window (380-480 nm), consistent with theory. The increasing prevalence of white lasers, angle filter-based wavelength scanning, and large, sensitive multi-anode photo-multiplier tubes make acquisition of such MuSIC-compatible datasets increasingly attainable.

An excitation wavelength–scanning spectral imaging system for preclinical imaging

2008 ◽  
Vol 79 (2) ◽  
pp. 023707 ◽  
Author(s):  
Silas Leavesley ◽  
Yanan Jiang ◽  
Valery Patsekin ◽  
Bartek Rajwa ◽  
J. Paul Robinson
Keyword(s):  
Imaging System ◽  
Spectral Imaging ◽  
Excitation Wavelength ◽  
Preclinical Imaging ◽  
Wavelength Scanning

Preparation and Properties of La2O3-CaO-P2O5 Glasses Doped with Eu3+ Ions

2016 ◽  
Vol 675-676 ◽  
pp. 376-379 ◽  
Author(s):  
Narun Luewarasirikul ◽  
Piyachat Meejitpaisan ◽  
Jakrapong Kaewkhao
Keyword(s):  
Calcium Phosphate ◽  
Molar Volume ◽  
Absorption Spectra ◽  
Room Temperature ◽  
Emission Spectra ◽  
Phosphate Glasses ◽  
Excitation Wavelength ◽  
Melt Quenching ◽  
Volume Measurements ◽  
Doped Glasses

Lanthanum calcium phosphate glasses doped with Eu3+ ions in compositions 20La2O3:10CaO:(70-x)P2O5:xEu2O3 (where x = 0.05, 0.10, 0.50 and 1.50 mol%) were prepared by melt-quenching technique. The density and molar volume measurements were carried out at room temperature. The absorption spectra were investigated in the UV-Vis-NIR region from 200 to 2500 nm. The emission spectra of Eu3+-doped glasses centered at 590 nm (5D0→7F1), 612 nm (5D0→7F2), 652 nm (5D0→7F3) and 699 nm (5D0→7F4) have been observed with 393 nm excitation wavelength.

Non-Markovianity leading to coherence revivals in an open quantum system

2020 ◽  
pp. 2050040
Author(s):  
Y. Yugra ◽  
F. De Zela
Keyword(s):  
Quantum Information ◽  
Quantum System ◽  
Open Quantum System ◽  
Open Systems ◽  
Quantum Correlations ◽  
Experimental Proof ◽  
Optical Setup ◽  
Open Quantum ◽  
All Optical ◽  
Proof Of Principle

Coherence and quantum correlations have been identified as fundamental resources for quantum information tasks. As recently shown, these resources can be interconverted. In multipartite systems, entanglement represents a prominent case among quantum correlations, one which can be activated from coherence. All this makes coherence a key resource for securing the operational advantage of quantum technologies. When dealing with open systems, decoherence hinders full exploitation of quantum resources. Here, we present a protocol that allows reaching the maximal achievable amount of coherence in an open quantum system. By implementing our protocol, or suitable variants of it, coherence losses might be fully compensated, thereby leading to coherence revivals. We provide an experimental proof of principle of our protocol through its implementation with an all-optical setup.

Multiple excitation wavelength fluorescence emission spectra technique for discrimination of phytoplankton

2010 ◽  
Vol 9 (1) ◽  
pp. 16-24 ◽  
Author(s):  
Xupeng Hu ◽  
Rongguo Su ◽  
Fang Zhang ◽  
Xiulin Wang ◽  
Hongtao Wang ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Emission Spectra ◽  
Excitation Wavelength ◽  
Fluorescence Emission Spectra

scholarly journals Quantum Dots—Assisted 2D Fluorescence for Pattern Based Sensing of Amino Acids, Oligopeptides and Neurotransmitters

Sensors ◽  
2019 ◽  
Vol 19 (17) ◽  
pp. 3655 ◽  
Author(s):  
Marcin Zabadaj ◽  
Patrycja Ciosek-Skibińska
Keyword(s):  
Amino Acids ◽  
Quantum Dots ◽  
Emission Spectra ◽  
Specific Interactions ◽  
High Quantum Yield ◽  
Huge Amount ◽  
Broad Absorption ◽  
Amount Of Information ◽  
Decay Times ◽  
Proof Of Principle

Quantum dots (QDs) are very attractive nanomaterials for analytical chemistry, due to high photostability, large surface area featuring numerous ways of bioconjugation with biomolecules, usually high quantum yield and long decay times. Their broad absorption spectra and narrow, sharp emission spectra of size-tunable fluorescence make them ideal tools for pattern-based sensing. However, almost always they are applied for specific sensing with zero-dimensional (0D) signal reporting (only peak heights or peak shifts are considered), without taking advantage of greater amount of information hidden in 1D signal (emission spectra), or huge amount of information hidden in 2D fluorescence maps (Excitation-Emission Matrixes, EEMs). Therefore, in this work we propose opposite strategy—non-specific interactions of QDs, which are usually avoided and regarded as their disadvantage, were exploited here for 2D fluorescence fingerprinting. Analyte-specific multivariate fluorescence response of QDs is decoded with the use of Partial Least Squares—Discriminant Analysis. Even though only one type of QDs is studied, the proposed pattern-based method enables to obtain satisfactory accuracy for all studied compounds—various neurotransmitters, amino-acids and oligopeptides. This is a proof of principle of the possibility of the identification of various bioanalytes by such fluorescence fingerprinting with the use of QDs.

scholarly journals Concentration Quantification of Oil Samples by Three-Dimensional Concentration-Emission Matrix (CEM) Spectroscopy

2019 ◽  
Vol 10 (1) ◽  
pp. 315
Author(s):  
Yunan Chen ◽  
Ruifang Yang ◽  
Nanjing Zhao ◽  
Wei Zhu ◽  
Yao Huang ◽  
...  
Keyword(s):  
Oil Spill ◽  
Three Dimensional ◽  
Emission Spectra ◽  
Quantitative Detection ◽  
Excitation Wavelength ◽  
Detection Technology ◽  
Shift Behavior ◽  
Relative Errors ◽  
Matrix Similarity

Developing fast and accurate fluorescence detection technology of oil spill is significant for quantitative analysis in unexpected oil spill events. As the oil sample concentration increases, the fluorescence spectrum produces red-shift behavior, which seriously affects the quantitative detection of concentration. In this work, a three-dimensional concentration-emission matrix (CEM) was constructed by using a series of emission spectra with different levels of concentration at the excitation wavelength of 266 nm. The database is the interpolated CEM of six samples using bicubic interpolation in the concentration dimension. With matrix similarity matching, the database was used to achieve quantification of the concentration of oil samples. The recovery rates of prediction for test samples and weathering samples of six oil samples were between 86.8% and 116.11%, with relative errors of predictions ranging from 2.09% to 15.2%. The results show that this method can provide accurate quantitative determination of the concentration of different oil samples.

Preparation and Luminescent Properties of Nancrystalline Zro2:Eu3+ by Emulsion Method

2011 ◽  
Vol 393-395 ◽  
pp. 244-248
Author(s):  
Yan Lin Chen ◽  
Ming Yan ◽  
Yi Ming Sun ◽  
Jiao Qun Zhu
Keyword(s):  
Size Structure ◽  
Sintering Temperature ◽  
Emission Spectra ◽  
Luminescent Properties ◽  
Blue Shift ◽  
Processing Method ◽  
Spectral Emission ◽  
Emulsion Processing ◽  
Main Emission Peak ◽  
Material Grain Size

Europium-doped nanocrystalline ZrO2: Eu3+ powders with different doped concentrations were prepared by emulsion processing method, with different doped concentration and calcination temperature on the material grain size, structure, size and luminescent properties. The results show that: Eu3+ has a stabilizing effect on tetragonal phase of ZrO2. Emission spectra indicates that the main emission peak in the 593m and 608m, “Blue Shift “will occur in 5D0 → 7F2 transition of the emission spectra of sample with the sintering temperature at 1000°C. Samples with the sintering temperature soaring, spectral emission intensity also increases.

DIELECTRIC AND OPTICAL BEHAVIOR OF STRONTIUM DOPED TiO2 SYNTHESIZED BY SOLID STATE REACTION METHOD

2013 ◽  
Vol 22 ◽  
pp. 336-339
Author(s):  
M. H. MANGROLA ◽  
V. G. JOSHI ◽  
B. H. PARMAR ◽  
A. S. PILLAI
Keyword(s):  
Solid State ◽  
Solid State Reaction ◽  
Emission Spectra ◽  
Solid State Reaction Method ◽  
Excitation Wavelength ◽  
Solid State Reaction Technique ◽  
Dielectric Constant And Loss ◽  
Pl Emission ◽  
Optical Applications ◽  
Optical Behavior

In the present study, Strontium doped Titanium Dioxide (TiO2) were synthesized by standard solid-state reaction technique. The dielectric property of the synthesized samples has been studied in the present work. The dielectric constant and loss have been measured as a function of frequency (100 Hz-1 KHz) and temperature (50°C to 300°C). Strontium doped TiO2 shows interesting optical properties promising a variety of optical applications. Photoluminescence (PL) spectra was obtained at different excitation wavelength. PL emission spectra show that positions of PL peaks gradually shifts towards the violet region with increase of Sr ion dosage from 3% to 5%. The results are discussed in details.

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