An excitation wavelength–scanning spectral imaging system for preclinical imaging

2008 ◽  
Vol 79 (2) ◽  
pp. 023707 ◽  
Author(s):  
Silas Leavesley ◽  
Yanan Jiang ◽  
Valery Patsekin ◽  
Bartek Rajwa ◽  
J. Paul Robinson
Keyword(s):  
Imaging System ◽  
Spectral Imaging ◽  
Excitation Wavelength ◽  
Preclinical Imaging ◽  
Wavelength Scanning

scholarly journals Fluorescence Multiplexing with Spectral Imaging and Combinatorics

2018 ◽  
Author(s):  
Hadassa Y. Holzapfel ◽  
Alan D. Stern ◽  
Mehdi Bouhaddou ◽  
Catilin M. Anglin ◽  
Danielle Putur ◽  
...  
Keyword(s):  
Emission Spectra ◽  
Spectral Imaging ◽  
Excitation Wavelength ◽  
Spectral Emission ◽  
Experimental Proof ◽  
Measurement Tools ◽  
Wavelength Scanning ◽  
Covalently Linked ◽  
Proof Of Principle

ABSTRACTUltraviolet-to-infrared fluorescence is a versatile and accessible assay modality, but is notoriously hard to multiplex due to overlap of wide emission spectra. We present an approach for fluorescence multiplexing using spectral imaging and combinatorics (MuSIC). MuSIC consists of creating new independent probes from covalently-linked combinations of individual fluorophores, leveraging the wide palette of currently available probes with the mathematical power of combinatorics. Probe levels in a mixture can be inferred from spectral emission scanning data. Theory and simulations suggest MuSIC can increase fluorescence multiplexing ~4-5 fold using currently available dyes and measurement tools. Experimental proof-of-principle demonstrates robust demultiplexing of nine solution-based probes using ~25% of the available excitation wavelength window (380-480 nm), consistent with theory. The increasing prevalence of white lasers, angle filter-based wavelength scanning, and large, sensitive multi-anode photo-multiplier tubes make acquisition of such MuSIC-compatible datasets increasingly attainable.

scholarly journals Analysis of Compound Stained Cervical Cell Images Using Multi-Spectral Imaging

2021 ◽  
Vol 11 (12) ◽  
pp. 5628
Author(s):  
Run Fang ◽  
Libo Zeng ◽  
Fan Yi
Keyword(s):  
Cervical Cancer ◽  
Mouse Liver ◽  
Imaging System ◽  
Color Image ◽  
Imaging Technique ◽  
Screening Method ◽  
Spectral Imaging ◽  
High Accuracy ◽  
Cervical Cell ◽  
Real World Applications

Multi-spectral imaging technique plays an important role in real-world applications such as medicine and medical detections. This paper proposes a cervical cancer cell screening method to simultaneously adopt TBS classification and DNA quantitative analysis for a single cell smear. Through using compound staining on a smear, the cytoplasm is stained by Papanicolauo and the nucleus is stained by Feulgen. The main evaluation parameter is the DNA content of the nucleus, not the subjective description of cell morphology, which is more objective than the TBS classification method and reduces the chances of missing a diagnosis due to subjective factors. Each nucleus has its own DI value and color image of the whole cell, which is convenient for doctors as it allows them to review and confirm the morphology of cells with a nucleus DI of over 2.5. Mouse liver smears and cervical cases are utilized as the measuring specimens to evaluate the performance of the microscope multi-spectral imaging system; illustrative results demonstrate that the proposed system qualifies, with high accuracy and reliability, and further presents wide application prospects in the early diagnosis of cervical cancer.

UAV based Multi-spectral Imaging System for Environmental Monitoring

2011 ◽  
Vol 78 (11) ◽  
pp. 503-507 ◽  
Author(s):  
Martin De Biasio ◽  
T. Arnold ◽  
R. Leitner
Keyword(s):  
Environmental Monitoring ◽  
Imaging System ◽  
Spectral Imaging

Research on filter selection method for broadband spectral imaging system based on ancient murals

2015 ◽  
Vol 41 (6) ◽  
pp. 585-595 ◽  
Author(s):  
Jinxing Liang ◽  
Xiaoxia Wan ◽  
Qiang Liu ◽  
Chan Li ◽  
Junfeng Li
Keyword(s):  
Imaging System ◽  
Spectral Imaging ◽  
Selection Method ◽  
Filter Selection

scholarly journals Miniature Compressive Ultra-spectral Imaging System Utilizing a Single Liquid Crystal Phase Retarder

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Isaac August ◽  
Yaniv Oiknine ◽  
Marwan AbuLeil ◽  
Ibrahim Abdulhalim ◽  
Adrian Stern
Keyword(s):  
Liquid Crystal ◽  
Imaging System ◽  
Spectral Imaging ◽  
Crystal Phase ◽  
Liquid Crystal Phase

Design of a Hyper-Spectral Imaging System for Gross Pathology of Pigmented Skin Lesions

2021 ◽  
Author(s):  
Eleni Aloupogianni ◽  
Masahiro Ishikawa ◽  
Takaya Ichimura ◽  
Atsushi Sasaki ◽  
Naoki Kobayashi ◽  
...  
Keyword(s):  
Imaging System ◽  
Spectral Imaging ◽  
Skin Lesions ◽  
Gross Pathology ◽  
Hyper Spectral ◽  
Hyper Spectral Imaging ◽  
Pigmented Skin Lesions

Dyson spectral imaging system with large relative aperture and wide spectral region

2013 ◽  
Vol 21 (10) ◽  
pp. 2535-2542 ◽  
Author(s):  
薛庆生 薛庆生 ◽  
王淑荣 王淑荣 ◽  
于向阳 于向阳
Keyword(s):  
Spectral Region ◽  
Imaging System ◽  
Spectral Imaging
Sign in / Sign up

Export Citation Format

Share Document