scholarly journals Light dynamically regulates growth rate and cellular organisation of the Arabidopsis root meristem

2018 ◽  
Author(s):  
Thomas Blein ◽  
Jasmin Duerr ◽  
Taras Pasternak ◽  
Thomas Haser ◽  
Thorsten Falk ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Volume ◽  
Large Scale ◽  
Cell Length ◽  
Root Tip ◽  
Meristematic Zone ◽  
Proliferation Zone ◽  
Dark Light ◽  
Zone Length ◽  
Arabidopsis Root

1AbstractLarge-scale methods and robust algorithms are needed for a quantitative analysis of cells status/geometry in situ. It allows the understanding the cellular mechanisms that direct organ growth in response to internal and environmental cues. Using advanced whole-stack imaging in combination with pattern analysis, we have developed a new approach to investigate root zonation under different dark/light conditions. This method is based on the determination of 3 different parameters: cell length, cell volume and cell proliferation on the cell-layer level. This method allowed to build a precise quantitative 3D cell atlas of the Arabidopsis root tip. Using this approach we showed that the meristematic (proliferation) zone length differs between cell layers. Considering only the rapid increase of cortex cell length to determine the meristematic zone overestimates of the proliferation zone for epidermis/cortex and underestimates it for pericycle. The use of cell volume instead of cell length to define the meristematic zone correlates better with cell proliferation zone.

scholarly journals An efficient direct screening system for microorganisms that activate plant immune responses based on plant–microbe interactions using cultured plant cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mari Kurokawa ◽  
Masataka Nakano ◽  
Nobutaka Kitahata ◽  
Kazuyuki Kuchitsu ◽  
Toshiki Furuya
Keyword(s):  
Immune Responses ◽  
Plant Defense ◽  
Pseudomonas Syringae ◽  
Large Scale ◽  
Plant Cells ◽  
Defense Responses ◽  
Root Tip ◽  
General Strategy ◽  
Plant Defense Responses ◽  
Microbe Interactions

AbstractMicroorganisms that activate plant immune responses have attracted considerable attention as potential biocontrol agents in agriculture because they could reduce agrochemical use. However, conventional methods to screen for such microorganisms using whole plants and pathogens are generally laborious and time consuming. Here, we describe a general strategy using cultured plant cells to identify microorganisms that activate plant defense responses based on plant–microbe interactions. Microbial cells were incubated with tobacco BY-2 cells, followed by treatment with cryptogein, a proteinaceous elicitor of tobacco immune responses secreted by an oomycete. Cryptogein-induced production of reactive oxygen species (ROS) in BY-2 cells served as a marker to evaluate the potential of microorganisms to activate plant defense responses. Twenty-nine bacterial strains isolated from the interior of Brassica rapa var. perviridis plants were screened, and 8 strains that enhanced cryptogein-induced ROS production in BY-2 cells were selected. Following application of these strains to the root tip of Arabidopsis seedlings, two strains, Delftia sp. BR1R-2 and Arthrobacter sp. BR2S-6, were found to induce whole-plant resistance to bacterial pathogens (Pseudomonas syringae pv. tomato DC3000 and Pectobacterium carotovora subsp. carotovora NBRC 14082). Pathogen-induced expression of plant defense-related genes (PR-1, PR-5, and PDF1.2) was enhanced by the pretreatment with strain BR1R-2. This cell–cell interaction-based platform is readily applicable to large-scale screening for microorganisms that enhance plant defense responses under various environmental conditions.

scholarly journals The Arabidopsis Root Tip (Phospho)Proteomes at Growth-Promoting versus Growth-Repressing Conditions Reveal Novel Root Growth Regulators

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1665
Author(s):  
Natalia Nikonorova ◽  
Evan Murphy ◽  
Cassio Flavio Fonseca de Lima ◽  
Shanshuo Zhu ◽  
Brigitte van de Cotte ◽  
...  
Keyword(s):  
Cell Wall ◽  
Root Growth ◽  
Growth Regulators ◽  
Growth Regulation ◽  
Phosphorylation Site ◽  
Primary Root ◽  
Root Tip ◽  
Arabidopsis Root ◽  
Growth Promoting ◽  
The Impact

Auxin plays a dual role in growth regulation and, depending on the tissue and concentration of the hormone, it can either promote or inhibit division and expansion processes in plants. Recent studies have revealed that, beyond transcriptional reprogramming, alternative auxin-controlled mechanisms regulate root growth. Here, we explored the impact of different concentrations of the synthetic auxin NAA that establish growth-promoting and -repressing conditions on the root tip proteome and phosphoproteome, generating a unique resource. From the phosphoproteome data, we pinpointed (novel) growth regulators, such as the RALF34-THE1 module. Our results, together with previously published studies, suggest that auxin, H+-ATPases, cell wall modifications and cell wall sensing receptor-like kinases are tightly embedded in a pathway regulating cell elongation. Furthermore, our study assigned a novel role to MKK2 as a regulator of primary root growth and a (potential) regulator of auxin biosynthesis and signalling, and suggests the importance of the MKK2 Thr31 phosphorylation site for growth regulation in the Arabidopsis root tip.

scholarly journals Experimental Study on Backflow Patterns Induced by a Bilateral Groin Pair with Different Spacing

2021 ◽  
Vol 11 (4) ◽  
pp. 1486
Author(s):  
Cuiping Kuang ◽  
Yuhua Zheng ◽  
Jie Gu ◽  
Qingping Zou ◽  
Xuejian Han
Keyword(s):  
Cross Sections ◽  
Large Scale ◽  
Recirculation Zone ◽  
Flow Direction ◽  
Wake Flow ◽  
Zone Length ◽  
Array Configuration ◽  
Contraction Ratio ◽  
River Training ◽  
Spanwise Velocity

Groins are one of the popular manmade structures to modify the hydraulic flow and sediment response in river training. The spacing between groins is a critical consideration to balance the channel-depth and the cost of construction, which is generally determined by the backflow formed downstream from groins. A series of experiments were conducted using Particle Image Velocimetry (PIV) to observe the influence of groin spacing on the backflow pattern of two bilateral groins. The spacing between groins has significant effect on the behavior of the large-scale recirculation cell behind groins. The magnitude of the wake flow induced by a groin was similar to that induced by another groin on the other side, but the flow direction is opposite. The spanwise velocity near the groin tip dictates the recirculation zone width behind the groins due to the strong links between the spanwise velocity and the contraction ratio of channel cross-sections between groins. Based on previous studies and present experimental results, quantitative empirical relationships are proposed to calculate the recirculation zone length behind groins alternately placed at different spacing along riverbanks. This study provides better understanding and a robust formula to assess the backflow extent of alternate groins and identify the optimum groins array configuration.

scholarly journals YAP Promotes Cell Proliferation and Stemness Maintenance of Porcine Muscle Stem Cells under High-Density Condition

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3069
Author(s):  
Zheng Liu ◽  
Ling Lin ◽  
Haozhe Zhu ◽  
Zhongyuan Wu ◽  
Xi Ding ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Large Scale ◽  
High Density ◽  
Meat Production ◽  
Density Condition ◽  
High Density Culture ◽  
Differentiation Potential ◽  
Muscle Stem Cells ◽  
Cultured Meat

Muscle stem cells (MuSCs) isolated ex vivo are essential original cells to produce cultured meat. Currently, one of the main obstacles for cultured meat production derives from the limited capacity of large-scale amplification of MuSCs, especially under high-density culture condition. Here, we show that at higher cell densities, proliferation and differentiation capacities of porcine MuSCs are impaired. We investigate the roles of Hippo-YAP signaling, which is important regulators in response to cell contact inhibition. Interestingly, abundant but not functional YAP proteins are accumulated in MuSCs seeded at high density. When treated with lysophosphatidic acid (LPA), the activator of YAP, porcine MuSCs exhibit increased proliferation and elevated differentiation potential compared with control cells. Moreover, constitutively active YAP with deactivated phosphorylation sites, but not intact YAP, promotes cell proliferation and stemness maintenance of MuSCs. Together, we reveal a potential molecular target that enables massive MuSCs expansion for large-scale cultured meat production under high-density condition.

Aquaporin 2-increased renal cell proliferation is associated with cell volume regulation

2012 ◽  
Vol 113 (12) ◽  
pp. 3721-3729 ◽  
Author(s):  
Gisela Di Giusto ◽  
Pilar Flamenco ◽  
Valeria Rivarola ◽  
Juan Fernández ◽  
Luciana Melamud ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Volume ◽  
Renal Cell ◽  
Volume Regulation ◽  
Cell Volume Regulation ◽  
Aquaporin 2

scholarly journals Mapping sites of gibberellin biosynthesis in the Arabidopsis root tip

2020 ◽  
Vol 229 (3) ◽  
pp. 1521-1534
Author(s):  
Richard Barker ◽  
Maria Nieves Fernandez Garcia ◽  
Stephen J. Powers ◽  
Simon Vaughan ◽  
Malcolm J. Bennett ◽  
...  
Keyword(s):  
Root Tip ◽  
Gibberellin Biosynthesis ◽  
Arabidopsis Root

scholarly journals RML1 and RML2, Arabidopsis Genes Required for Cell Proliferation at the Root Tip

1995 ◽  
Vol 107 (2) ◽  
pp. 365-376 ◽  
Author(s):  
J. C. Cheng ◽  
K. A. Seeley ◽  
Z. R. Sung
Keyword(s):  
Cell Proliferation ◽  
Root Tip
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