scholarly journals Rhamnolipids fromPseudomonas aeruginosaDisperse the Biofilms of Sulfate-Reducing Bacteria

2018 ◽  
Author(s):  
Thammajun L. Wood ◽  
Lei Zhu ◽  
James Miller ◽  
Daniel S. Miller ◽  
Bei Yin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Genetic Basis ◽  
Metal Corrosion ◽  
Desulfovibrio Vulgaris ◽  
Sulfate Reducing ◽  
Drilling Equipment ◽  
Biofilm Dispersal ◽  
Reducing Bacteria ◽  
Minimum Medium

ABSTRACTBiofilm formation is an important problem for many industries.Desulfovibrio vulgarisis the representative sulfate-reducing bacterium (SRB) which causes metal corrosion in oil wells and drilling equipment, and the corrosion is related to its biofilm formation. Biofilms are extremely difficult to remove since the cells are cemented in a polymer matrix. In an effort to eliminate SRB biofilms, we examined the ability of supernatants fromPseudomonas aeruginosaPA14 to disperse SRB biofilms. We found that theP. aeruginosasupernatants dispersed more than 98% of the biofilm. To determine the genetic basis of this SRB biofilm dispersal, we examined a series ofP. aeruginosamutants and found that mutantsrhlA,rhlB,rhlI, andrhlR,defective in rhamnolipids production, had significantly reduced levels of SRB biofilm dispersal. Corroborating these results, purified rhamnolipids dispersed SRB biofilms, and rhamnolipids were detected in theP. aeruginosasupernatants. Hence,P. aeruginosasupernatants disperse SRB biofilms via rhamnolipids. In addition, the supernatants ofP. aeruginosadispersed the SRB biofilms more readily than protease in M9 glucose minimum medium and were also effective against biofilms ofEscherichia coliandBacillus subtilis.

scholarly journals Unintended Laboratory-Driven Evolution Reveals Genetic Requirements for Biofilm Formation by Desulfovibrio vulgaris Hildenborough

mBio ◽  
2017 ◽  
Vol 8 (5) ◽  
Author(s):  
Kara B. De León ◽  
Grant M. Zane ◽  
Valentine V. Trotter ◽  
Gregory P. Krantz ◽  
Adam P. Arkin ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Sulfate Reducing Bacteria ◽  
Structural Proteins ◽  
Desulfovibrio Vulgaris ◽  
Type I ◽  
Nucleotide Change ◽  
Single Nucleotide ◽  
Sulfate Reducing ◽  
Single Nucleotide Change ◽  
Reducing Bacteria

ABSTRACT Biofilms of sulfate-reducing bacteria (SRB) are of particular interest as members of this group are culprits in corrosion of industrial metal and concrete pipelines as well as being key players in subsurface metal cycling. Yet the mechanism of biofilm formation by these bacteria has not been determined. Here we show that two supposedly identical wild-type cultures of the SRB Desulfovibrio vulgaris Hildenborough maintained in different laboratories have diverged in biofilm formation. From genome resequencing and subsequent mutant analyses, we discovered that a single nucleotide change within DVU1017, the ABC transporter of a type I secretion system (T1SS), was sufficient to eliminate biofilm formation in D. vulgaris Hildenborough. Two T1SS cargo proteins were identified as likely biofilm structural proteins, and the presence of at least one (with either being sufficient) was shown to be required for biofilm formation. Antibodies specific to these biofilm structural proteins confirmed that DVU1017, and thus the T1SS, is essential for localization of these adhesion proteins on the cell surface. We propose that DVU1017 is a member of the lapB category of microbial surface proteins because of its phenotypic similarity to the adhesin export system described for biofilm formation in the environmental pseudomonads. These findings have led to the identification of two functions required for biofilm formation in D. vulgaris Hildenborough and focus attention on the importance of monitoring laboratory-driven evolution, as phenotypes as fundamental as biofilm formation can be altered. IMPORTANCE The growth of bacteria attached to a surface (i.e., biofilm), specifically biofilms of sulfate-reducing bacteria, has a profound impact on the economy of developed nations due to steel and concrete corrosion in industrial pipelines and processing facilities. Furthermore, the presence of sulfate-reducing bacteria in oil wells causes oil souring from sulfide production, resulting in product loss, a health hazard to workers, and ultimately abandonment of wells. Identification of the required genes is a critical step for determining the mechanism of biofilm formation by sulfate reducers. Here, the transporter by which putative biofilm structural proteins are exported from sulfate-reducing Desulfovibrio vulgaris Hildenborough cells was discovered, and a single nucleotide change within the gene coding for this transporter was found to be sufficient to completely stop formation of biofilm.

scholarly journals Crude oil-utilizing strain Desulfovibrio vulgaris D107G3, a mesophilic sulfate-reducing bacterium isolated from Bach Ho gas-oil field in Vung Tau, Vietnam

2020 ◽  
Vol 12 (2) ◽  
pp. 193-199
Author(s):  
Thi Thu Huyen NGUYEN ◽  
Thi Kim Thoa TRAN ◽  
Thuy Hien LAI
Keyword(s):  
16S Rrna ◽  
Crude Oil ◽  
Sulfate Reducing Bacteria ◽  
Oil Field ◽  
Metal Corrosion ◽  
Desulfovibrio Vulgaris ◽  
Viet Nam ◽  
Gas Oil ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Some of anaerobic, mesophilic sulfate-reducing bacteria that produce H2S and cause microbial metal corrosion can degrade crude oil in anaerobic conditions. In this study, a mesophilic sulfate-reducing bacterial strain D107G3 isolated from Bach Ho gas-oil field in Vung Tau, Vietnam that is able to utilize crude oil in the anaerobic condition is reported. The strain D107G3 was classified as a Gram-negative bacterium by using Gram staining method. Basing on scanning microscopy observation, the cell of a strain D107G3 had a curved rod shape. The 16S rRNA gene sequence analysis showed that the strain D107G3 was identified as Desulfovibrio vulgaris with 99.7% identity. The suitable conditions for its growth that was determined via estimating its H2S production was the modified Postgate B medium containing 1% (v/v) crude oil, 1% NaCl (w/v), pH 7 and 30°C incubation. In these conditions, the strain D107G3 can consume 11.4 % of crude oil total and oxidize heavy crude oil (≥ C45) for one month at anoxic condition. These obtained results not only contribute to the science but also continue to warn about the dangers of mesophilic sulfate reducing bacteria to the process of crude oil exploitation, use, and storage in Vung Tau, Vietnam. Trong bài báo này, chủng vi khuẩn khử sunphat (KSF) ưa ấm D107G3 phân lập từ giếng khoan dầu khí mỏ Bạch Hổ, Vũng Tàu, Việt Nam có khả năng sử dụng dầu thô trong điều kiện kị khí được công bố. Chủng D107G3 được xác định là vi khuẩn Gram âm nhờ phương pháp nhuộm Gram. Quan sát trên kính hiển vi điện tử quét cho thấy tế bào chủng D107G3 có hình que cong. Kết quả phân tích trình tự gen 16S rRNA đã xác định được chủng D107G3 thuộc loài Desulfovibrio vulgaris với độ tương đồng 99.7%. Thông qua đánh giá lượng H2S tạo thành đã khám phá được điều kiện thích hợp cho sinh trưởng của chủng D107G3: môi trường Postgate B cải tiến chứa 1% (v/v) dầu thô, 1 % NaCl (gL-1), pH 7 và nuôi cấy ở 30°C. Trong điều kiện đó, chủng D107G3 đã sử dụng được 11.4 % hàm lượng dầu tổng số, thành phần dầu bị phân huỷ là các n-parafin có mạch C≥45 sau 1 tháng nuôi cấy kỵ khí. Các kết quả này đóng góp về mặt khoa học và tiếp tục cảnh báo mối nguy hại của KSF ưa ấm đến việc khai thác, sử dụng và bảo quản dầu mỏ ở Vũng Tàu, Việt Nam.

scholarly journals Effect of Quorum Sensing on the Ability of Desulfovibrio vulgaris To Form Biofilms and To Biocorrode Carbon Steel in Saline Conditions

2019 ◽  
Vol 86 (1) ◽  
Author(s):  
Giantommaso Scarascia ◽  
Robert Lehmann ◽  
Laura L. Machuca ◽  
Christina Morris ◽  
Ka Yu Cheng ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Carbon Steel ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Sulfate Reducing Bacteria ◽  
Desulfovibrio Vulgaris ◽  
Content Type ◽  
Sulfate Reducing ◽  
Alternative Approach ◽  
Reducing Bacteria

ABSTRACT Sulfate-reducing bacteria (SRB) are key contributors to microbe-induced corrosion (MIC), which can lead to serious economic and environmental impact. The presence of a biofilm significantly increases the MIC rate. Inhibition of the quorum-sensing (QS) system is a promising alternative approach to prevent biofilm formation in various industrial settings, especially considering the significant ecological impact of conventional chemical-based mitigation strategies. In this study, the effect of the QS stimulation and inhibition on Desulfovibrio vulgaris is described in terms of anaerobic respiration, cell activity, biofilm formation, and biocorrosion of carbon steel. All these traits were repressed when bacteria were in contact with QS inhibitors but enhanced upon exposure to QS signal molecules compared to the control. The difference in the treatments was confirmed by transcriptomic analysis performed at different time points after treatment application. Genes related to lactate and pyruvate metabolism, sulfate reduction, electron transfer, and biofilm formation were downregulated upon QS inhibition. In contrast, QS stimulation led to an upregulation of the above-mentioned genes compared to the control. In summary, these results reveal the impact of QS on the activity of D. vulgaris, paving the way toward the prevention of corrosive SRB biofilm formation via QS inhibition. IMPORTANCE Sulfate-reducing bacteria (SRB) are considered key contributors to biocorrosion, particularly in saline environments. Biocorrosion imposes tremendous economic costs, and common approaches to mitigate this problem involve the use of toxic and hazardous chemicals (e.g., chlorine), which raise health and environmental safety concerns. Quorum-sensing inhibitors (QSIs) can be used as an alternative approach to inhibit biofilm formation and biocorrosion. However, this approach would only be effective if SRB rely on QS for the pathways associated with biocorrosion. These pathways would include biofilm formation, electron transfer, and metabolism. This study demonstrates the role of QS in Desulfovibrio vulgaris on the above-mentioned pathways through both phenotypic measurements and transcriptomic approach. The results of this study suggest that QSIs can be used to mitigate SRB-induced corrosion problems in ecologically sensitive areas.

scholarly journals Microbial Corrosion of API 5L X-70 Carbon Steel by ATCC 7757 and Consortium of Sulfate-Reducing Bacteria

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Arman Abdullah ◽  
Nordin Yahaya ◽  
Norhazilan Md Noor ◽  
Rosilawati Mohd Rasol
Keyword(s):  
Weight Loss ◽  
Carbon Steel ◽  
Corrosion Rate ◽  
Sulfate Reducing Bacteria ◽  
Desulfovibrio Vulgaris ◽  
Corrosion Morphology ◽  
Sulfate Reducing ◽  
Weight Loss Method ◽  
Loss Method ◽  
Reducing Bacteria

Various cases of accidents involving microbiology influenced corrosion (MIC) were reported by the oil and gas industry. Sulfate reducing bacteria (SRB) have always been linked to MIC mechanisms as one of the major causes of localized corrosion problems. In this study, SRB colonies were isolated from the soil in suspected areas near the natural gas transmission pipeline in Malaysia. The effects of ATCC 7757 and consortium of isolated SRB upon corrosion on API 5L X-70 carbon steel coupon were investigated using a weight loss method, an open circuit potential method (OCP), and a potentiodynamic polarization curves method in anaerobic conditions. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were then used to determine the corrosion morphology in verifying the SRB activity and corrosion products formation. Results from the study show that the corrosion rate (CR) of weight loss method for the isolated SRB is recorded as 0.2017 mm/yr compared to 0.2530 mm/yr for ATCC 7757. The Tafel plot recorded the corrosion rate of 0.3290 mm/yr for Sg. Ular SRB and 0.2500 mm/yr forDesulfovibrio vulgaris. The results showed that the consortia of isolated SRB were of comparable effects and features with the single ATCC 7757 strain.

Identification of the genes for hydrogenase and cytochrome c3 in Desulfovibrio

1987 ◽  
Vol 33 (11) ◽  
pp. 1006-1010 ◽  
Author(s):  
Gerrit Voordouw ◽  
Helen M. Kent ◽  
John R. Postgate
Keyword(s):  
Cytochrome C ◽  
Sequence Divergence ◽  
Desulfovibrio Vulgaris ◽  
Gene Probe ◽  
Cytochrome C3 ◽  
Sulfate Reducing ◽  
Genes Encoding ◽  
Reducing Bacteria ◽  
Cryptic Plasmids ◽  
Amino Acid Sequence Divergence

Cloned genes encoding cytochrome c3 and hydrogenase from Desulfovibrio vulgaris Hildenborough have been used to probe the genomes of 15 other desulfovibrios. The D. vulgaris strains Wandle and Brockhurst Hill cannot be distinguished from the Hildenborough strain by Southern hybridization using either probe, indicating similar genomes. Desulfovibrio vulgaris Groningen is completely different and lacks homologous cytochrome c3 and hydrogenase genes. The genomes of D. vulgaris ssp. oxamicus Monticello and D. desulfuricans strains El Agheila Z, Berre sol, and Canet 41 contain genes encoding a homologous but not identical periplasmic hydrogenase and cytochrome c3. Weak hybridization was observed with the cytochrome c3 gene probe for genomes of seven other sulfate-reducing bacteria, which reflects the known amino acid sequence divergence of cytochrome c3 in Desulfovibrio. The hydrogenase gene probe shows weak hybridization to the DNA from two strains of D. salexigens only, while the gene may be absent from D. vulgaris Groningen, two strains of D. africanus, D. thermophilus, D. gigas, and D. desulfuricans strains Norway and Teddington R. In desulfovibrios carrying cryptic plasmids the cytochrome c3 and hydrogenase genes are apparently chromosomal.

The Disinfection Efficacy of Chlorine on Sulfate-Reducing Bacteria and Iron Bacteria in Water Supply Systems

2013 ◽  
Vol 316-317 ◽  
pp. 657-660
Author(s):  
Bei Meng Qi ◽  
Bei Jia Wang ◽  
Chen Guang Wu ◽  
Yi Xing Yuan
Keyword(s):  
Water Supply ◽  
Sulfate Reducing Bacteria ◽  
Metal Corrosion ◽  
Water Supply Systems ◽  
Supply Networks ◽  
Minimum Requirement ◽  
Iron Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria ◽  
Supply Systems

Sulfate reducing bacteria (SRB) and iron reducing bacteria (IRB) that widely exist in water supply networks are the main microorganisms leading to metal corrosion in pipelines. Chlorine is widely used in drinking water supply systems. The concentration of chlorine with SRB declined rapidly after 10 mins and reached 0 mg/L finally whereas it decreased more slowly with IRB. If the concentration of chlorine is lower than 0.2mg/L, IRB cannot be sterilized. It indicates that at the end of water pipes where the concentration of chlorine is required to be 0.05mg/L, chlorine is not effective since the concentration is below the minimum requirement of removing IRB

scholarly journals Induction of native c-di-GMP phosphodiesterases leads to dispersal of Pseudomonas aeruginosa biofilms

2021 ◽  
Author(s):  
Jens Bo Andersen ◽  
Kasper Nørskov Kragh ◽  
Louise Dahl Hultqvist ◽  
Morten Rybtke ◽  
Martin Nilsson ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Single Cell ◽  
Biofilm Formation ◽  
Ectopic Expression ◽  
Second Messenger ◽  
Flow Cell ◽  
Diguanylate Cyclases ◽  
Biofilm Dispersal ◽  
High Level

A decade of research has shown that the molecule c-di-GMP functions as a central second messenger in many bacteria. A high level of c-di-GMP is associated with biofilm formation whereas a low level of c-di-GMP is associated with a planktonic single-cell bacterial lifestyle. C-di-GMP is formed by diguanylate cyclases and is degraded by specific phosphodiesterases. We have previously presented evidence that ectopic expression in Pseudomonas aeruginosa of the Escherichia coli phosphodiesterase YhjH results in biofilm dispersal. More recently, however, evidence has been presented that induction of native c-di-GMP phosphodiesterases does not lead to dispersal of P. aeruginosa biofilms. The latter result may discourage attempts to use c-di-GMP signaling as a target for development of anti-biofilm drugs. However, here we demonstrate that induction of the P. aeruginosa c-di-GMP phosphodiesterases PA2133 and BifA indeed does result in dispersal of P. aeruginosa biofilms in both a microtiter tray biofilm assay and in a flow-cell biofilm system.

Microbiologically induced corrosion of stainless steel by Desulfovibrio vulgaris: An scanning electron microscope study

1991 ◽  
Vol 49 ◽  
pp. 28-29
Author(s):  
Roger Garcia ◽  
Fang Li ◽  
Lester Hendrickson
Keyword(s):  
Stainless Steel ◽  
Electron Microscope Study ◽  
Desulfovibrio Vulgaris ◽  
Biological Cell ◽  
Scanning Electron Microscope Study ◽  
Sulfate Reducing ◽  
Corrosion Cell ◽  
Reducing Bacteria ◽  
Microbiologically Induced Corrosion ◽  
Scanning Electron

The corrosion of mild steel by sulfate reducing bacteria has been studied quite extensively. However, with the replacement of mild steels with stainless steel in many of these applications numerous sightings of corroding stainless steel have been made as well. Initially, the cathodic depolarization theory was widely accepted as the mechanism for both. The essential part of this theory involves the removal of hydrogen from the metal surface. Hydrogenase in Desulfovibrio allows utilization of elemental hydrogen from the cathode of the corrosion cell. This causes the reduction of sulfate whereby the biological cell gets its energy via a respiration process. Finally, the oxygen from the sulfate becomes available to the cathode and hence corrosion is enhanced. Without this reducing action the cathode would become polarized thereby decreasing the EMF and lowering the corrosion rate. Among other proposed mechanisms are differential aeration cells and corrosive products produced by the bacteria.

scholarly journals Effect of the Deletion of qmoABC and the Promoter-Distal Gene Encoding a Hypothetical Protein on Sulfate Reduction in Desulfovibrio vulgaris Hildenborough

2010 ◽  
Vol 76 (16) ◽  
pp. 5500-5509 ◽  
Author(s):  
Grant M. Zane ◽  
Huei-che Bill Yen ◽  
Judy D. Wall
Keyword(s):  
Sulfate Reduction ◽  
Electron Acceptor ◽  
Transmembrane Protein ◽  
Hypothetical Protein ◽  
Desulfovibrio Vulgaris ◽  
Gene Encoding ◽  
Terminal Electron Acceptor ◽  
Sulfate Reducing ◽  
Reducing Bacteria

ABSTRACTThe pathway of electrons required for the reduction of sulfate in sulfate-reducing bacteria (SRB) is not yet fully characterized. In order to determine the role of a transmembrane protein complex suggested to be involved in this process, a deletion inDesulfovibrio vulgarisHildenborough was created by marker exchange mutagenesis that eliminated four genes putatively encoding the QmoABC complex and a hypothetical protein (DVU0851). The Qmo (quinone-interactingmembrane-boundoxidoreductase) complex is proposed to be responsible for transporting electrons to the dissimilatory adenosine-5′-phosphosulfate reductase in SRB. In support of the predicted role of this complex, the deletion mutant was unable to grow using sulfate as its sole electron acceptor with a range of electron donors. To explore a possible role for the hypothetical protein in sulfate reduction, a second mutant was constructed that had lost only the gene that codes for the DVU0851 protein. The second constructed mutant grew with sulfate as the sole electron acceptor; however, there was a lag that was not present with the wild-type or complemented strain. Neither deletion strain was significantly impaired for growth with sulfite or thiosulfate as the terminal electron acceptor. Complementation of the Δ(qmoABC-DVU0851) mutant with all four genes or only theqmoABCgenes restored its ability to grow by sulfate respiration. These results confirmed the prediction that the Qmo complex is in the electron pathway for sulfate reduction and revealed that no other transmembrane complex could compensate when Qmo was lacking.

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