scholarly journals Whole genome sequencing,de novoassembly and phenotypic profiling for the new budding yeast speciesSaccharomyces jurei

2018 ◽  
Author(s):  
Samina Naseeb ◽  
Haya Alsammar ◽  
Tim Burgis ◽  
Ian Donaldson ◽  
Norman Knyazev ◽  
...  

AbstractSaccharomyces sensu strictocomplex consist of yeast species, which are not only important in the fermentation industry but are also model systems for genomic and ecological analysis. Here, we present the complete genome assemblies ofSaccharomyces jurei,a newly discoveredSaccharomyces sensu strictospecies from high altitude oaks. Phylogenetic and phenotypic analysis revealed thatS. jureiis a sister-species toS. mikatae,thanS. cerevisiae,andS. paradoxus.The karyotype ofS. jureipresents two reciprocal chromosomal translocations between chromosome VI/VII and I/XIII when compared toS. cerevisiaegenome. Interestingly, while the rearrangement I/XIII is unique toS. jurei,the other is in common withS. mikataestrain IFO1815, suggesting shared evolutionary history of this species after the split betweenS. cerevisiaeandS. mikatae.The number of Ty elements differed in the new species, with a higher number of Ty elements present inS. jureithan inS. cerevisiae.Phenotypically, theS. jureistrain NCYC 3962 has relatively higher fitness than the other strain NCYC 3947Tunder most of the environmental stress conditions tested and showed remarkably increased fitness in higher concentration of acetic acid compared to the othersensu strictospecies. Both strains were found to be better adapted to lower temperatures compared toS. cerevisiae.

1998 ◽  
Vol 44 (11) ◽  
pp. 1045-1050 ◽  
Author(s):  
Gennadi I Naumov ◽  
Elena S Naumova ◽  
Paul D Sniegowski

Genetic hybridization and karyotypic analyses revealed the biological species Saccharomyces paradoxus and Saccharomyces cerevisiae in exudates from North American oaks for the first time. In addition, two strains collected from elm flux and from Drosophila by Phaff in 1961 and 1952 were reidentified as S. paradoxus. Each strain studied showed a unique profile of chromosomal hybridization with a probe for the retrotransposable element Ty1. The wild distribution of natural Saccharomyces sensu stricto yeasts is discussed.Key words: genetical taxonomy, Saccharomyces paradoxus, oak exudates, Ty elements, electrophoretic karyotyping.


1991 ◽  
Vol 37 (3) ◽  
pp. 74-79
Author(s):  
Vaughan A. MARTINI ◽  
A. MARTINI

2018 ◽  
Author(s):  
Casey M. Bergman

AbstractBackgroundRecent evidence suggests that horizontal transfer plays a significant role in the evolution of of transposable elements (TEs) in eukaryotes. Many cases of horizontal TE transfer (HTT) been reported in animals and plants, however surprisingly few examples of HTT have been reported in fungi.FindingsHere I report evidence for a novel HTT event in fungi involving Tsu4 in Saccharomyces paradoxus based on (i) high similarity between Tsu4 elements in S. paradoxus and S. uvarum, (ii) a patchy distribution of Tsu4 in S. paradoxus and general absence from its sister species S. cerevisiae, and (iii) discordance between the phylogenetic history of Tsu4 sequences and species in the Saccharomyces sensu stricto group. Available data suggests the HTT event likely occurred somewhere in the Nearctic, Neotropic or Indo-Australian part of the S. paradoxus species range, and that a lineage related to S. uvarum or S. eubayanus was the donor species. The HTT event has led to massive proliferation of Tsu4 in the South American lineage of S. paradoxus, which exhibits partial reproductive isolation with other strains of this species because of multiple reciprocal translocations. Full-length Tsu4 elements are associated with both breakpoints of one of these reciprocal translocations.ConclusionsThis work shows that comprehensive analysis of TE sequences in essentially-complete genome assemblies derived from long-read sequencing provides new opportunities to detect HTT events in fungi and other organisms. This work also provides support for the hypothesis that HTT and subsequent TE proliferation can induce genome rearrangements that contribute to post-zygotic isolation in yeast.


In 1924 one of us (C. D.), with the late Professor A. E. Boycott, F. R. S., collected random samples from populations of the polymorphic land snail Cepaea nemoralis L. on sand-dunes at Bundoran, County Donegal, Eire. In 1961, the other two revisited the area and sampled, as nearly as possible, the same localities. A total of 23857 live snails were collected and scored for various inherited shell characters. The data are considered from three points of view. First we have examined the distribution of morphs within individual samples, hoping to detect associations indicating linkage or selection. Secondly, we have studied the differences between contemporary samples and attempted to relate them to environmental factors. Thirdly, we have compared the two series (1924 and 1961) in order to detect any evolutionary changes that might have occurred. (1) Associations within samples In both series there are consistent linkage disequilibria involving the loci for shell-colour, for banded v. unbanded shell, for hyalozonate v. fully pigmented bands, and for white v. black lip. It is likely that these disequilibria are maintained by selection. There is also a suggestion of disequilibrium between the shell-colour locus and the loci determining the extent of band-fusions. We find no significant differences between adults and young in the proportions of any of the morphs. (2) Variation between samples Most of the characters show morph-ratio dines on an east/west axis. This pattern may be related to the fact that the western part of the area is predominantly mobile or semi-mobile dune, whereas the eastern part is generally more stable. The overall proportions of yellow and 'effectively unbanded’ shells very roughly correspond to those found by Cain & Sheppard (1954) hedgerows and rough herbage. They occupy the range of frequencies expected according to the hypothesis of visual selection by predators. Nevertheless, their precise distributions within the area are not evidently related to local changes of background, nor are the distribution of unbandeds ( sensu stricto ), hyalozonates, whites, white lips or fusions. There may possibly be come correspondence in the case of browns, 00300 and 00345, and it is conceivable that the local distribution of pinks is the result of visual selection by rabbits. A final interpretation must await further detailed studies of other sand-dune populations. (3) Comparison of the two series A comparison of the series collected in 1924 with that collected in 1961 provides no evidence of consistent evolutionary change. There may have been small local changes, which in our data would be indistinguishable from differences due to errors in relocating the sampling areas; but in general the populations seem to have remained stable. There have certainly been no changes comparable to those observed at Berrow, Somerset (Clarke & Murray 1962 a ). This apparent constancy may be related to the fact that the habitat at Bundoran has not greatly altered since 1924. At Berrow, on the other hand, the dunes have gradually become more stable, and larger parts of them have become overgrown with Hippophae . In each case the evolutionary situation seems to reflect the history of the habitat. It will clearly be of great interest to follow future developments at both localities.


Author(s):  
Colby Dickinson

In his somewhat controversial book Remnants of Auschwitz, Agamben makes brief reference to Theodor Adorno’s apparently contradictory remarks on perceptions of death post-Auschwitz, positions that Adorno had taken concerning Nazi genocidal actions that had seemed also to reflect something horribly errant in the history of thought itself. There was within such murderous acts, he had claimed, a particular degradation of death itself, a perpetration of our humanity bound in some way to affect our perception of reason itself. The contradictions regarding Auschwitz that Agamben senses to be latent within Adorno’s remarks involve the intuition ‘on the one hand, of having realized the unconditional triumph of death against life; on the other, of having degraded and debased death. Neither of these charges – perhaps like every charge, which is always a genuinely legal gesture – succeed in exhausting Auschwitz’s offense, in defining its case in point’ (RA 81). And this is the stance that Agamben wishes to hammer home quite emphatically vis-à-vis Adorno’s limitations, ones that, I would only add, seem to linger within Agamben’s own formulations in ways that he has still not come to reckon with entirely: ‘This oscillation’, he affirms, ‘betrays reason’s incapacity to identify the specific crime of Auschwitz with certainty’ (RA 81).


2017 ◽  
Vol 39 (2) ◽  
pp. 265-276 ◽  
Author(s):  
Kas Saghafi

In several late texts, Derrida meditated on Paul Celan's poem ‘Grosse, Glühende Wölbung’, in which the departure of the world is announced. Delving into the ‘origin’ and ‘history’ of the ‘conception’ of the world, this paper suggests that, for Derrida, the end of the world is determined by and from death—the death of the other. The death of the other marks, each and every time, the absolute end of the world.


2019 ◽  
Vol 188 (1) ◽  
pp. 95-146
Author(s):  
Martin Bohatý ◽  
Dalibor Velebil

Adalbert Wraný (*1836, †1902) was a doctor of medicine, with his primary specialization in pediatric pathology, and was also one of the founders of microscopic and chemical diagnostics. He was interested in natural sciences, chemistry, botany, paleontology and above all mineralogy. He wrote two books, one on the development of mineralogical research in Bohemia (1896), and the other on the history of industrial chemistry in Bohemia (1902). Wraný also assembled several natural science collections. During his lifetime, he gave to the National Museum large collections of rocks, a collection of cut precious stones and his library. He donated a collection of fossils to the Geological Institute of the Czech University (now Charles University). He was an inspector of the mineralogical collection of the National Museum. After his death, he bequeathed to the National Museum his collection of minerals and the rest of the gemstone collection. He donated paintings to the Prague City Museum, and other property to the Klar Institute of the Blind in Prague. The National Museum’s collection currently contains 4 325 samples of minerals, as well as 21 meteorites and several hundred cut precious stones from Wraný’s collection.


2019 ◽  
Vol 1 (1) ◽  
pp. 5-14
Author(s):  
Carlos Alvaréz Teijeiro

Emmanuel Lévinas, the philosopher of ethics par excellence in the twentieth century, and by own merit one of the most important ethical philosophers in the history of western philosophy, is also the philosopher of the Other. Thereby, it can be said that no thought has deepened like his in the ups and downs of the ethical relationship between subject and otherness. The general objective of this work is to expose in a simple and understandable way some ideas that tend to be quite dark in the philosophical work of the author, since his profuse religious production will not be analyzed here. It is expected to show that his ideas about the being and the Other are relevant to better understand interpersonal relationships in times of 4.0 (re)evolution. As specific objectives, this work aims to expose in chronological order the main works of the thinker, with special emphasis on his ethical implications: Of the evasion (1935), The time and the Other (1947), From the existence to the existent (1947), Totality and infinity: An essay on exteriority (1961) and, last, Otherwise than being, or beyond essence (1974). In the judgment of Lévinas, history of western philosophy starting with Greece, has shown an unusual concern for the Being, this is, it has basically been an ontology and, accordingly, it has relegated ethics to a second or third plane. On the other hand and in a clear going against the tide movement, our author supports that ethics should be considered the first philosophy and more, even previous to the proper philosophize. This novel approach implies, as it is supposed, that the essential question of the philosophy slows down its origin around the Being in order to inquire about the Other: it is a philosophy in first person. Such a radical change of perspective generates an underlying change in how we conceive interpersonal relationships, the complex framework of meanings around the relationship Me and You, which also philosopher Martin Buber had already spoken of. As Lévinas postulates that ethics is the first philosophy, this involves that the Other claims all our attention, intellectual and emotional, to the point of considering that the relationship with the Other is one of the measures of our identity. Thus, “natural” attitude –husserlian word not used by Lévinas- would be to be in permanent disposition regarding to the meeting with the Other, to be in permanent opening state to let ourselves be questioned by him. Ontology, as the author says, being worried about the Being, has been likewise concerned about the Existence, when the matter is to concern about the particular Existent that every otherness supposes for us. In conclusion it can be affirmed that levinasian ethics of the meeting with the Other, particular Face, irreducible to the assumption, can contribute with an innovative looking to (re)evolving the interpersonal relationships in a 4.0 context.


Author(s):  
Juliana Martins de Mesquita Matos ◽  
Rosana De Carvalho Cristo Martins ◽  
Valéria Regina Bellotto ◽  
Lilian Gomes da Silva Rocha ◽  
Eloiza Aparecida Barbosa ◽  
...  

Dalbergia miscolobium or Jacarandá do Cerrado is a species of legume in the Fabaceae family. It occurs in the sensu stricto Cerrado and in the dystrophic cerradão. It shows potential for landscaping and for recovering damaged areas. It is an endangered species and therefore is protected by the law that prevents cut in areas of the Federal District (Decree No. 14.783/93). The purpose of this study was to determine the best procedure to prepare seeds of Dalbergia miscolobium to assess viability in the tetrazolium test. We carried out the following treatments: i) hydration on filter paper at 25 ° C, ii) hydration on filter paper at 25 ° C followed by a cut in the tegument and iii) hydration on filter paper at 25 ° C followed bya complete removal of the tegument. The results were analyzed using analysis of variance and the Tukey range test. The analyzes showed that the best procedure to prepare seeds of Dalbergia miscolobium is the treatment in which there is a hydration followed by the complete removal of the integument. Where 78% of the seeds showed uniform staining, indicating that the seeds analyzed are of good quality. The other treatments, hydration and hydration followed by cutting, showed respectively 35% and 41% of viable seeds. RESUMO A Dalbergia miscolobium ou Jacarandá do Cerrado é uma espécie de leguminosa da família Fabaceae. Ocorre no sentido stricto Cerrado e no cerradão distrófico. Possui potencial para paisagismo e para recuperar áreas degradadas. É uma espécie ameaçada de extinção e, portanto, está protegida pela lei que previne o corte em áreas do Distrito Federal (Decreto 14.783 / 93). O objetivo deste estudo foi determinar o melhor procedimento de prepararação das sementes de Dalbergia miscolobium para serem submetidas à análise de viabilidade pelo teste de tetrazólio. Foram realizados os seguintes tratamentos: i) hidratação em papel de filtro a 25 ° C, ii) hidratação em papel de filtro a 25 ° C seguida de um corte no tegumento e iii) hidratação em papel de filtro a 25 ° C seguido de remoção completa do tegumento. Os resultados foram analisados utilizando-se a análise de variância e o teste de médias de Tukey. As análises mostraram que o melhor procedimento para preparar sementes de Dalbergia miscolobium é o tratamento em que há uma hidratação seguida pela remoção completa do tegumento, onde 78% das sementes apresentaram coloração uniforme, indicando que as sementes analisadas são de boa qualidade. Os demais tratamentos, hidratação e hidratação seguida de corte, mostraram respectivamente 35% e 41% de sementes viáveis.


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