scholarly journals Maternal tamoxifen treatment expands the macrophage population of early mouse embryos

2018 ◽  
Author(s):  
Rocío Rojo ◽  
Kristin A. Sauter ◽  
Lucas Lefevre ◽  
David A. Hume ◽  
Clare Pridans
Keyword(s):  
Mononuclear Phagocyte ◽  
Mouse Embryos ◽  
Tamoxifen Treatment ◽  
Macrophage Population ◽  
Large Expansion ◽  
Pregnant Mice ◽  
Early Mouse ◽  
Leukocyte Populations ◽  
Whole Mounts ◽  
Macrophage Lineage

AbstractSeveral different transgenic tamoxifen-inducible cre reporter lines have been used to analyse the contribution of embryonic precursors to the development of the mononuclear phagocyte system in mice. Here we show that tamoxifen treatment of the mother at 8.5dpc with doses commonly-used in lineage trace studies produces a 4-5-fold expansion of the embryonic leukocyte populations by 10.5dpc, detected in whole mounts of embryos using aCsf1rreporter gene or separately by expression ofCsf1r, Itgam(CD11b),Adgre1(F4/80) orPtprc(CD45) mRNA. These findings indicate that tamoxifen cannot be considered a neutral agonist in macrophage lineage trace studies.Summary sentenceTreatment of pregnant mice with tamoxifen in early gestation produces a large expansion of the embryonic macrophage population.

Sodium Fluoride Affects DNA Methylation of Imprinted Genes in Mouse Early Embryos

2015 ◽  
Vol 147 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Lei Zhao ◽  
Sheng Zhang ◽  
Xinglan An ◽  
Wentao Tan ◽  
Bo Tang ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Embryonic Development ◽  
Mrna Level ◽  
Mouse Embryos ◽  
Imprinted Genes ◽  
Male Mice ◽  
Early Embryos ◽  
Pregnant Mice ◽  
H19 Gene ◽  
Early Mouse

Fluorine is reported to affect embryonic development, but the underlining mechanism is unclear. The modification of DNA methylation of the H19 and Peg3 genes is important in embryonic development. Therefore, the effect of fluorine on methylation of H19 and Peg3 during early mouse embryos was studied. It was shown that the H19 gene was significantly downmethylated in E2.5, E3.5, and E4.5 embryos from pregnant mice treated with 120 mg/l NaF in drinking water for 48 h. But methylation of both H19 and Peg3 genes was disrupted when the parent male mice were treated with NaF for 35 days. H19 DNA methylation decreased significantly, while Peg3 was almost completely methylated. However, when pregnant mice, mated with NaF-treated male mice, were again treated with NaF for 48 h, either H19 or Peg3 methylation in the embryos decreased significantly. In addition, the mRNA level of H19 considerably increased in E3.5 and E4.5 embryos from NaF-treated pregnant mice. Further, the expression of DNMT1 decreased significantly after NaF treatment. Conclusively, we demonstrated that fluorine may adversely affect early embryonic development by disrupting the methylation of H19 and Peg3 through downregulation of DNMT1.

scholarly journals TCL1 promotes blastomere proliferation through nuclear transfer, but not direct phosphorylation, of AKT/PKB in early mouse embryos

2007 ◽  
Vol 15 (2) ◽  
pp. 420-422 ◽  
Author(s):  
M T Fiorenza ◽  
S Torcia ◽  
S Canterini ◽  
A Bevilacqua ◽  
M G Narducci ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Mouse Embryos ◽  
Early Mouse

scholarly journals The Explanation for the Blockade of Glycolysis in Early Mouse Embryos

1974 ◽  
Vol 71 (4) ◽  
pp. 1056-1060 ◽  
Author(s):  
E. K. Barbehenn ◽  
R. G. Wales ◽  
O. H. Lowry
Keyword(s):  
Mouse Embryos ◽  
Early Mouse

Cell division and cell allocation in early mouse development

Development ◽  
1978 ◽  
Vol 48 (1) ◽  
pp. 37-51
Author(s):  
S. J. Kelly ◽  
J. G. Mulnard ◽  
C. F. Graham
Keyword(s):  
Cell Division ◽  
Tritiated Thymidine ◽  
Mouse Embryos ◽  
Mouse Development ◽  
Stages Of Development ◽  
Cell Stage ◽  
Cell Cycles ◽  
Short Cell ◽  
Early Mouse

Cell division was observed in intact and dissociated mouse embryos between the 2-cell stage and the blastocyst in embryos developing in culture. Division to the 4-cell stage was usually asynchronous. The first cell to divide to the 4-cell stage produced descendants which tended to divide ahead of those cells produced by its slow partner at all subsequent stages of development up to the blastocyte stage. The descendants of the first cell to divide to the 4-cell stage did not subsequently have short cell cycles. The first cell or last cell to divide from the 4-cell stage was labelled with tritiated thymidine. The embryo was reassembled, and it was found that the first pair of cells to reach the 8-cell stage contributed disproportionately more descendants to the ICM when compared with the last cell to divide to the 8-cell stage.

Lethality of radioisotopes in early mouse embryos

Development ◽  
1979 ◽  
Vol 52 (1) ◽  
pp. 203-208
Author(s):  
Hilary A. Macqueen
Keyword(s):  
Mouse Embryos ◽  
Highly Sensitive ◽  
Path Lengths ◽  
Early Mouse

The development of pre-implantation mouse embryos was found to be prevented by exposure of the embryos to [35S]methionine, but not to [3H]methionine. Such embryos have also been shown to be highly sensitive to [3H]thymidine. These observations are discussed with reference to the path lengths and energies of electrons emitted from the different radioisotopes.

Effects of tunicamycin upon glycoprotein synthesis and development of early mouse embryos

1980 ◽  
Vol 79 (1) ◽  
pp. 19-32 ◽  
Author(s):  
Sui Bi Atienza-Samols ◽  
Perla Razon Pine ◽  
Michael I. Sherman
Keyword(s):  
Mouse Embryos ◽  
Glycoprotein Synthesis ◽  
Early Mouse

Colchicine-induced Heteroploidy in Early Mouse Embryos

Nature ◽  
1954 ◽  
Vol 174 (4423) ◽  
pp. 276-277 ◽  
Author(s):  
R. G. EDWARDS
Keyword(s):  
Mouse Embryos ◽  
Early Mouse
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