scholarly journals Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis

2018 ◽  
Author(s):  
Lucia Durrieu ◽  
Daniel Kirrmaier ◽  
Tatjana Schneidt ◽  
Ilia Kats ◽  
Sarada Raghavan ◽  
...  
Keyword(s):  
Developmental Biology ◽  
Quantitative Analysis ◽  
Formation Mechanism ◽  
Novel Approach ◽  
Gradient Formation ◽  
Lifetime Analysis ◽  
Abundance And Distribution ◽  
Insight Into ◽  
Anterior Posterior ◽  
Rule Out

AbstractEmbryogenesis relies on instructions provided by spatially organized signaling molecules known as morphogens. Understanding the principles behind morphogen distribution and how cells interpret locally this information remains a major challenge in developmental biology. Here we introduce morphogen-age measurements as a novel approach to retrieve key parameters in morphogen dynamics. Using a tandem fluorescent timer (tFT) as a protein-age sensor we find a gradient of increasing age of Bicoid (Bcd) along the anterior-posterior (AP) axis in the early Drosophila embryo. Quantitative analysis retrieves parameter that are most consistent with the synthesis-diffusion-degradation (SDD) model underlying Bcd-gradient formation, and rule out some other hypotheses for gradient formation. Moreover, we show that the timer can detect transitions in the dynamics associated with syncytial cellularization. Our results provide new insight into Bcd gradient formation, and demonstrate how morphogen age-information can complement knowledge about movement, abundance and distribution, which should be widely applicable for other systems.

scholarly journals Bicoid gradient formation mechanism and dynamics revealed by protein lifetime analysis

2018 ◽  
Vol 14 (9) ◽  
Author(s):  
Lucia Durrieu ◽  
Daniel Kirrmaier ◽  
Tatjana Schneidt ◽  
Ilia Kats ◽  
Sarada Raghavan ◽  
...  
Keyword(s):  
Formation Mechanism ◽  
Gradient Formation ◽  
Lifetime Analysis

Quantitative Analysis Of X-Ray Images From Geological Materials

1990 ◽  
Vol 48 (2) ◽  
pp. 244-245
Author(s):  
J. M. Paque ◽  
R. Browning ◽  
P. L. King ◽  
P. Pianetta
Keyword(s):  
Quantitative Analysis ◽  
Bulk Composition ◽  
Principal Component ◽  
Analytical Description ◽  
Bulk Sample ◽  
Geological Samples ◽  
X Ray ◽  
Software And Hardware ◽  
And Storage ◽  
Insight Into

Geological samples typically contain many minerals (phases) with multiple element compositions. A complete analytical description should give the number of phases present, the volume occupied by each phase in the bulk sample, the average and range of composition of each phase, and the bulk composition of the sample. A practical approach to providing such a complete description is from quantitative analysis of multi-elemental x-ray images.With the advances in recent years in the speed and storage capabilities of laboratory computers, large quantities of data can be efficiently manipulated. Commercial software and hardware presently available allow simultaneous collection of multiple x-ray images from a sample (up to 16 for the Kevex Delta system). Thus, high resolution x-ray images of the majority of the detectable elements in a sample can be collected. The use of statistical techniques, including principal component analysis (PCA), can provide insight into mineral phase composition and the distribution of minerals within a sample.

New insight into the formation mechanism of 2-furfurylthiol in the glucose-cysteine reaction with ribose

2021 ◽  
Vol 143 ◽  
pp. 110295
Author(s):  
Huan Liu ◽  
Zhenyu Wang ◽  
Teng Hui ◽  
Fei Fang ◽  
Dequan Zhang
Keyword(s):  
Formation Mechanism ◽  
Insight Into

New insight into the molecular switch mechanism of human Rho family proteins: shifting a paradigm

2013 ◽  
Vol 394 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Mamta Jaiswal ◽  
Eyad Kalawy Fansa ◽  
Radovan Dvorsky ◽  
Mohammad Reza Ahmadian
Keyword(s):  
Quantitative Analysis ◽  
Bound State ◽  
Molecular Switch ◽  
Nucleotide Exchange ◽  
Gtp Binding ◽  
Switch Mechanism ◽  
Rho Family ◽  
Rho Family Proteins ◽  
Insight Into ◽  
Made In

Abstract Major advances have been made in understanding the structure, function and regulation of the small GTP-binding proteins of the Rho family and their involvement in multiple cellular process and disorders. However, intrinsic nucleotide exchange and hydrolysis reactions, which are known to be fundamental to Rho family proteins, have been partially investigated in the case of RhoA, Rac1 and Cdc42, but for others not at all. Here we present a comprehensive and quantitative analysis of the molecular switch functions of 15 members of the Rho family that enabled us to propose an active GTP-bound state for the rather uncharacterized isoforms RhoD and Rif under equilibrium and quiescent conditions.

Anodic stripping voltammetry of anti-Vi antibody functionalized CdTe quantum dots for the specific monitoring of Salmonella enterica serovar Typhi

RSC Advances ◽  
2015 ◽  
Vol 5 (107) ◽  
pp. 88234-88240 ◽  
Author(s):  
Satish K. Pandey ◽  
Praveen Rishi ◽  
C. Raman Suri ◽  
Aaydha C. Vinayaka
Keyword(s):  
Quantum Dots ◽  
Capsular Polysaccharide ◽  
Anodic Stripping Voltammetry ◽  
Stripping Voltammetry ◽  
Cdte Quantum Dots ◽  
Salmonella Enterica Serovar Typhi ◽  
Novel Approach ◽  
Anodic Stripping ◽  
Vi Capsular Polysaccharide ◽  
Insight Into

CdTe QD based stripping voltammetry for Vi capsular polysaccharide detection. The technique has provided an insight into the competence of CdTe QD and GNP immuno-conjugates. This is a novel approach to characterize the efficiency of immuno-conjugates of QDs and GNPs.

scholarly journals Dreibens Modulo 7: A New Formula for Primality Testing

Elements ◽  
2016 ◽  
Vol 12 (1) ◽  
Author(s):  
Arthur Diep-Nguyen
Keyword(s):  
Number Theory ◽  
Linear Algebra ◽  
Abstract Algebra ◽  
Additional Insight ◽  
Primality Testing ◽  
New Formula ◽  
Insight Into ◽  
Rule Out

In this paper, we discuss strings of 3’s and 7’s, hereby dubbed “dreibens.” As a first step towards determining whether the set of prime dreibens is infinite, we examine the properties of dreibens when divided by 7. by determining the divisibility of a dreiben by 7, we can rule out some composite dreibens in the search for prime dreibens. We are concerned with the number of dreibens of length n that leave a remainder i when divided by 7. By using number theory, linear algebra, and abstract algebra, we arrive at a formula that tells us how many dreibens of length n are divisible by 7. We also find a way to determine the number of dreibens of length n that leave a remainder i when divided by 7. Further investigation from a combinatorial perspective provides additional insight into the properties of dreibens when divided by 7. Overall, this paper helps characterize dreibens, opens up more paths of inquiry into the nature of dreibens, and rules out some composite dreibens from a prime dreiben search.

scholarly journals The use of a fixed high sensitivity to evaluate five D-dimer assays' ability to rule out deep venous thrombosis: a novel approach

2005 ◽  
Vol 131 (3) ◽  
pp. 341-347 ◽  
Author(s):  
Scott M. Stevens ◽  
C. Gregory Elliott ◽  
Scott C. Woller ◽  
Liang Li ◽  
Sterling T. Bennett ◽  
...  
Keyword(s):  
Venous Thrombosis ◽  
Deep Venous Thrombosis ◽  
High Sensitivity ◽  
Novel Approach ◽  
D Dimer ◽  
Rule Out

Fruit developmental biology and endosperm rumination in Elaeocarpus ruminatus (Elaeocarpaceae), and its taxonomic significance

2018 ◽  
Author(s):  
Janet N. Gagul ◽  
David Y. P. Tng ◽  
Darren M. Crayn
Keyword(s):  
Developmental Biology ◽  
Adaptive Significance ◽  
Evolutionary Significance ◽  
Phylogenetic Distribution ◽  
Taxonomic Significance ◽  
The Family ◽  
Developmental Phases ◽  
Trees And Shrubs ◽  
Insight Into ◽  
Gaining Insight

The genus Elaeocarpus is the largest genus in the family Elaeocarpaceae, comprising more than 350 species of trees and shrubs with a mainly Indo-Pacific distribution. Approximately 28 species in the genus, including nine species from Australia, are known to possess ruminate endosperm. To provide a basis for understanding fruit development and endosperm rumination in the genus and, therefore, its taxonomic and evolutionary significance, we studied the fruit anatomy of Elaeocarpus ruminatus F.Muell. at different developmental phases (petal-fall to maturity). We found lignin in pericarp and ovary wall tissues in the earliest stages of development. In contrast, endosperm rumination occurs only after fruits have fully expanded, and becomes more pronounced as fruits ripen. Its phylogenetic distribution suggests that ruminate endosperm is a derived, albeit homoplasious character in Elaeocarpus. Comparative studies on related species will be instructive in determining the utility of ruminate endosperm for informing infra-generic taxonomy of the genus, and gaining insight into its adaptive significance.

Mathematical Resolution of MBTI Creativity Data Into Personality Type Components

1993 ◽  
Author(s):  
Douglass J. Wilde
Keyword(s):  
Quantitative Analysis ◽  
Design Education ◽  
Personality Type ◽  
Personality Types ◽  
Dimensional Vector ◽  
Coordinate Systems ◽  
Psychological Issues ◽  
Novel Approach ◽  
Usual Manner ◽  
American Society

Abstract A novel approach to the quantitative analysis of psychological issues in design is formulated. It treats any quadruple of Myers-Briggs Personality Index (MBTI) scores as a four-dimensional vector decomposable into components in the usual manner of vector analysis. To make the MBTI scores more understandable to the engineer untrained in psychology, new coordinate systems are proposed in which the component axes point toward human personality types such as “Strategist” rather than the usual idealized psychological concepts such as “Introvert”. Also presented as a semantic aid to understanding is a tentative matrix of oversimplified word labels serving as a bridge between terse psychological designators such as “INTJ” and their corresponding detailed personality type descriptions. The methods are used to analyze results from two American Society for Engineering Education (ASEE) faculty workshops on creativity in design education.

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