scholarly journals Cold shock as a screen for genes involved in cold acclimatization in Neurospora crassa

2018 ◽  
Author(s):  
Michael K. Watters ◽  
Victor Manzanilla ◽  
Holly Howell ◽  
Alexander Mehreteab ◽  
Erik Rose ◽  
...  
Keyword(s):  
Cold Shock ◽  
Genetic Characterization ◽  
Morphological Changes ◽  
Morphological Response ◽  
Significant Percentage ◽  
E Coli ◽  
Cold Environments ◽  
Cold Shock Response ◽  
Shock Response

ABSTRACTWhen subjected to rapid drops of temperature (cold shock), Neurospora responds with a dramatic, but temporary shift in its branching pattern. While the cold shock response has been described morphologically, it has yet to be examined genetically. This project aims to begin the genetic characterization of the cold shock response and the associated acclimatization to cold environments. We report here the results of a screen of mutants from the Neurospora knockout library for alterations in their morphological response to cold shock and thus, their ability to acclimatize to the cold. Three groups of knockouts were selected to be subject to this screen: genes previously suspected to be involved in hyphal development as well as knockouts resulting in morphological changes; transcription factors; and genes homologous to E. coli genes known to alter their expression in response to cold shock. Several strains were identified with altered responses. The genes impacted in these mutants are listed and discussed. A significant percentage (81%) of the knockouts of genes homologous to those previously identified in E. coli showed altered cold shock responses in Neurospora – suggesting that the response in these two organisms is largely shared in common.

CspB and CspC are induced upon cold shock in Bacillus cereus strain D2

2021 ◽  
Author(s):  
Haoyang Li ◽  
Rui Yang ◽  
Linlin Hao ◽  
Chunli Wang ◽  
Mingtang Li
Keyword(s):  
Bacillus Cereus ◽  
Contaminated Soils ◽  
Cold Shock ◽  
Amino Acid Sequences ◽  
Cold Shock Protein ◽  
E Coli ◽  
Subsequent Transformation ◽  
Cold Shock Response ◽  
Shock Response ◽  
Psychrotrophic Strain

Bacillus cereus D2, a psychrotrophic strain, plays an essential role in the restoration of heavy metal-contaminated soils, especially at low temperatures. However, the cold shock response mechanisms of this strain are unclear. In this study, the cold shock response of B. cereus D2 was characterized; as per the Arrhenius curve, 10 °C was chosen as the cold shock temperature. Six cold shock-like proteins were found and temporarily named cold shock protein (Csp)1-6; the respective genes were cloned and identified. Quantitative real-time PCR results showed that csp1, csp2, csp3, and csp6 were overexpressed under cold shock conditions. Interestingly, after cloning the respective encoding genes into pET-28a (+) vector and their subsequent transformation into E. coli BL21 (DE3), the strains expressing Csp2 and Csp6 grew faster at 10 °C, showing a large number of bacteria. These results suggest that Csp2 and Csp6 are the major cold shock proteins in B. cereus D2. Of note, the comparison of amino acid sequences and structures showed that Csp2 and Csp6 belong to the CspB and CspC families, respectively. Additionally, we show that the number of hydrophobic residues is not a determining feature of major Csps, while, on the other hand, the formation of an α-helix in the context of a leucine residue is the most dominant difference between major, and other Bacillus and E. coli Csps.

The Cold Shock Response of Lactobacillus casei: Relation between HPr Phosphorylation and Resistance to Freeze/Thaw Cycles

2007 ◽  
Vol 13 (1-3) ◽  
pp. 65-75 ◽  
Author(s):  
Sophie Beaufils ◽  
Nicolas Sauvageot ◽  
Alain Mazé ◽  
Jean-Marie Laplace ◽  
Yanick Auffray ◽  
...  
Keyword(s):  
Lactobacillus Casei ◽  
Cold Shock ◽  
Freeze Thaw ◽  
Cold Shock Response ◽  
Shock Response

scholarly journals The CLO3403/CLO3404 Two-Component System of Clostridium botulinum E1 Beluga Is Important for Cold Shock Response and Growth at Low Temperatures

2013 ◽  
Vol 80 (1) ◽  
pp. 399-407 ◽  
Author(s):  
Gerald Mascher ◽  
Yağmur Derman ◽  
David G. Kirk ◽  
Eveliina Palonen ◽  
Miia Lindström ◽  
...  
Keyword(s):  
Clostridium Botulinum ◽  
Cold Shock ◽  
Response Regulator ◽  
Growth Efficiency ◽  
Content Type ◽  
Cold Shock Response ◽  
Two Component ◽  
Shock Response ◽  
Negative Effect ◽  
Group Ii

ABSTRACTIn order to survive a temperature downshift, bacteria have to sense the changing environment and adjust their metabolism and structure. Two-component signal transduction systems (TCSs) play a central role in sensing and responding to many different environmental stimuli. Although the nonproteolytic (group II)Clostridium botulinumrepresents a major hazard in chilled foods, the cold adaption mechanisms of group IIC. botulinumorganisms are not known. Here, we show that the CLO3403/CLO3404 TCS ofC. botulinumE1 Beluga is involved in the cold shock response and growth at 12°C. Cold shock induced the expression of the genes encoding the histidine kinase (clo3403) and the response regulator (clo3404) by more than 100-fold after 5 h relative to their expression in a nonshocked culture at the corresponding time point. The involvement of CLO3403/CLO3404 in growth at low temperature was demonstrated by impaired growth of the insertionalclo3403andclo3404knockout mutants at 12°C compared to the growth of the wild-type culture. Additionally, the inactivation ofclo3403had a negative effect on motility. The growth efficiency at 12°C of the TCS mutants and the motility of the kinase mutants were restored by introducing a plasmid harboring the operon of the CLO3403/CLO3404 TCS. The results suggest that the CLO3403/CLO3404 TCS is important for the cold tolerance ofC. botulinumE1 Beluga.

The cold shock response inLactococcus lactis subsp.lactis

1994 ◽  
Vol 29 (4) ◽  
pp. 213-216 ◽  
Author(s):  
Jean-Michel Panoff ◽  
Stéphane Legrand ◽  
Bouachanh Thammavongs ◽  
Philippe Boutibonnes
Keyword(s):  
Cold Shock ◽  
Cold Shock Response ◽  
Shock Response

scholarly journals Genomewide Transcriptional Analysis of the Cold Shock Response in Bacillus subtilis

2002 ◽  
Vol 184 (22) ◽  
pp. 6395-6402 ◽  
Author(s):  
Carsten L. Beckering ◽  
Leif Steil ◽  
Michael H. W. Weber ◽  
Uwe Völker ◽  
Mohamed A. Marahiel
Keyword(s):  
Bacillus Subtilis ◽  
Cold Shock ◽  
Research Field ◽  
Transcriptional Analysis ◽  
Two Component System ◽  
Two Dimensional Gel Electrophoresis ◽  
Component System ◽  
Cold Shock Response ◽  
Two Component ◽  
Shock Response

ABSTRACT Previous studies with two-dimensional gel electrophoresis techniques revealed that the cold shock response in Bacillus subtilis is characterized by rapid induction and accumulation of two classes of specific proteins, which have been termed cold-induced proteins (CIPs) and cold acclimatization proteins (CAPs), respectively. Only recently, the B. subtilis two-component system encoded by the desKR operon has been demonstrated to be essential for the cold-induced expression of the lipid-modifying desaturase Des, which is required for efficient cold adaptation of the membrane in the absence of isoleucine. At present, one of the most intriguing questions in this research field is whether DesKR plays a global role in cold signal perception and transduction in B. subtilis. In this report, we present the first genomewide transcriptional analysis of a cold-exposed bacterium and demonstrate that the B. subtilis two-component system DesKR exclusively controls the desaturase gene des and is not the cold-triggered regulatory system of global relevance. In addition to this, we identified a set of genes that might participate as novel players in the cold shock adaptation of B. subtilis. Two cold-induced genes, the elongation factor homolog ylaG and the σL-dependent transcriptional activator homolog yplP, have been examined by construction and analysis of deletion mutants.

Cold-shock response and cold-shock proteins

1999 ◽  
Vol 2 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Sangita Phadtare ◽  
Janivette Alsina ◽  
Masayori Inouye
Keyword(s):  
Cold Shock ◽  
Cold Shock Proteins ◽  
Cold Shock Response ◽  
Shock Response ◽  
Shock Proteins
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