Phenotypic and genetic characterization of enteropathogenic Escherichia coli (EPEC) and enteroaggregative E. coli (EAEC) from diarrhoeal and non-diarrhoeal children in Libya

2006 ◽  
Vol 29 (2-3) ◽  
pp. 100-113 ◽  
Author(s):  
M.A. Dow ◽  
I. Tóth ◽  
A. Malik ◽  
M. Herpay ◽  
N. Nógrády ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Characterization ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Phenotypic And Genetic Characterization

scholarly journals Genetic Characterization of IncI2 Plasmids CarryingblaCTX-M-55Spreading in both Pets and Food Animals in China

2013 ◽  
Vol 57 (6) ◽  
pp. 2824-2827 ◽  
Author(s):  
Luchao Lv ◽  
Sally R. Partridge ◽  
Liangying He ◽  
Zhenling Zeng ◽  
Dandan He ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Genetic Characterization ◽  
Content Type ◽  
E Coli ◽  
Food Animals ◽  
Plasmid Backbone

ABSTRACTpHN1122-1 carryingblaCTX-M-55, from anEscherichia coliisolate from a dog, was completely sequenced. pHN1122-1 has an IncI2 replicon and typical IncI2-associated genetic modules, includingmok/hok-finO-yafA/B,nikABC, and two transfer regions,traandpil, as well as a shufflon.blaCTX-M-55is found within a 3.084-kb ISEcp1transposition unit that includes a fragment of IncA/C plasmid backbone. pHN1122-1 and closely related plasmids were identified in otherE. coliisolates from animals in China.

scholarly journals Genetic Characterization of Escherichia coli O104 Isolates from Different Sources in the United States

2011 ◽  
Vol 78 (5) ◽  
pp. 1615-1618 ◽  
Author(s):  
Lydia V. Rump ◽  
Sonya Bodeis-Jones ◽  
Jason Abbott ◽  
Shaohua Zhao ◽  
Julie Kase ◽  
...  
Keyword(s):  
United States ◽  
Escherichia Coli ◽  
Virulence Genes ◽  
Genetic Characterization ◽  
The United States ◽  
Content Type ◽  
E Coli ◽  
Virulence Markers ◽  
Different Sources

ABSTRACTEscherichia coliO104 isolates collected from different sources in the United States were examined for virulence genes typical of enterohemorrhagicE. coliand those identified in the O104:H4 isolate associated with the 2011 German outbreak. The unexpected presence of virulence markers in these isolates highlights the importance of screening unusual and potentially pathogenic Shiga toxin-producingE. coliserotypes.

scholarly journals Detection and Characterization of Shiga ToxigenicEscherichia coli by Using Multiplex PCR Assays forstx 1, stx 2,eaeA, Enterohemorrhagic E. coli hlyA,rfb O111, andrfb O157

1998 ◽  
Vol 36 (2) ◽  
pp. 598-602 ◽  
Author(s):  
Adrienne W. Paton ◽  
James C. Paton
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Genetic Characterization ◽  
Gastrointestinal Disease ◽  
Diverse Group ◽  
E Coli ◽  
Pcr Products ◽  
Uremic Syndrome ◽  
Pcr Assays

Shiga toxigenic Escherichia coli (STEC) comprises a diverse group of organisms capable of causing severe gastrointestinal disease in humans. Within the STEC family, certain strains appear to be of greater virulence for humans, for example, those belonging to serogroups O111 and O157 and those with particular combinations of other putative virulence traits. We have developed two multiplex PCR assays for the detection and genetic characterization of STEC in cultures of feces or foodstuffs. Assay 1 utilizes four PCR primer pairs and detects the presence of stx 1,stx 2 (including variants ofstx 2), eaeA, and enterohemorrhagicE. coli hlyA, generating amplification products of 180, 255, 384, and 534 bp, respectively. Assay 2 uses two primer pairs specific for portions of the rfb (O-antigen-encoding) regions of E. coli serotypes O157 and O111, generating PCR products of 259 and 406 bp, respectively. The two assays were validated by testing 52 previously characterized STEC strains and observing 100% agreement with previous results. Moreover, assay 2 did not give a false-positive O157 reaction with enteropathogenic E. colistrains belonging to clonally related serogroup O55. Assays 1 and 2 detected STEC of the appropriate genotype in primary fecal cultures from five patients with hemolytic-uremic syndrome and three with bloody diarrhea. Thirty-one other primary fecal cultures from patients without evidence of STEC infection were negative.

scholarly journals Molecular and phenotypic typing of enteropathogenic Escherichia coli isolated in childhood acute diarrhea in Abuja, Nigeria

2017 ◽  
Vol 11 (07) ◽  
pp. 527-535 ◽  
Author(s):  
Casmir Ifeanyichukwu Cajetan Ifeanyi ◽  
Nkiruka Florence Ikeneche ◽  
Bassey Enya Bassey ◽  
Stefano Morabito ◽  
Caterina Graziani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acute Diarrhea ◽  
Antimicrobial Agents ◽  
Virulence Gene ◽  
Enteropathogenic Escherichia Coli ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Typical Epec ◽  
Marked Resistance

Introduction: Enteropathogenic Escherichia coli (EPEC) causes infectious diarrhea among children in developing countries. However, in Nigeria, due to limited laboratory resources, the genetic diversity of its virulence factors, which include intimin subtypes, remains undefined. Methodology: EPEC isolates from diarrheic children 60 months of age and younger in Abuja, Nigeria, were analyzed. Polymerase chain reaction (PCR) for EPEC virulence gene, Hep-2 cell adherence, and serotyping were performed. EPEC strains were further subtyped by PCR for the identification of intimin subtype genes α (alpha), β (beta), γ1 (gamma-1), and έ (epsilon). Antibiotic resistance and extended-spectrum beta-lactamase (ESBL) production was determined by Clinical and Laboratory Standards Institute guidelines. Results: Overall, 18 (4.5%) out of 400 children with acute diarrhea had EPEC infection.  Typical EPEC (tEPEC) strains were detected in 14 (3.5%), whereas 4 (1.1%) were atypical EPEC (aEPEC). A total of 15 (83.3%) of the EPEC isolated belonged to β intimin subtype gene, while the remaining 3 EPEC isolates possessed the intimin έ subtype. No α and γ intimin subtypes were detected. Traditional EPEC serotypes O114:H14 were detected only in tEPEC strains. Marked resistance to β-lactam agents were observed but no ESBL-producing tEPEC or aEPEC was detected. Conclusions: This is the first report of intimin subtype genes in Abuja, Nigeria. EPEC isolates of diverse serotypes resistant to β-lactam antimicrobial agents were observed. These data will be useful in facilitating the characterization of intimin variants of EPEC and some Shiga toxin-producing E. coli (STEC) in humans and other animal species.

scholarly journals Genetic Characterization of Plasmid-mediated qepA Gene Among ESBL-producing Escherichia Coli Isolates in Mexico

2021 ◽  
Author(s):  
Humberto Barrios-Camacho ◽  
Josefina Duran-Bedolla ◽  
Jesus Silva-Sanchez ◽  
Luis Lozano-Aguirre ◽  
Fernando Reyna-Flores ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Genetic Characterization ◽  
Insertion Sequences ◽  
Plasmid Sequence ◽  
E Coli ◽  
Multiple Copies ◽  
Chromosomal Mutations ◽  
Sequence Types

Abstract A molecular characterization of a plasmid-born qepA gene in (ESBL)-producing E. coli clinical isolates were performed. An 2.63% (11/418) were qepA positive isolates, of which a 90.0% carried CTX-M-15 (9/11) and SHV-12 (1/11). All isolates showed chromosomal mutations in the gyrA and parC genes. The clonal groups A, B and C were identified and belonged to, respectively, phylogroups A, B1 and D, as well as the sequence types 205, 405 and 617. Several plasmid profiles were determined with incompatibility groups FIA, FIB and FII. The genetic environment of the qepA in plasmid pEC8020 was different from those reported previously. The plasmid sequence included genes conferring resistance to β-lactams (blaCTX-M-15), macrolides (mphA), fluoroquinolones (qepA1), trimethoprim (dfrB4) and sulphonamides (sul1). Likewise, the IncF-pEC8020 plasmid carried several insertion sequences including ISCR3, IS6100 and multiple copies of IS26. This work contributes to the epidemiology and genetics of plasmid-born qepA genes of ESBL-producing E. coli.

scholarly journals Generation of Escherichia coli O9a Serotype, a Subtype of E. coli O9, by Transfer of the wb* Gene Cluster of Klebsiella O3 into E. colivia Recombination

1998 ◽  
Vol 180 (10) ◽  
pp. 2775-2778 ◽  
Author(s):  
Tsuyoshi Sugiyama ◽  
Nobuo Kido ◽  
Yutaka Kato ◽  
Naoki Koide ◽  
Tomoaki Yoshida ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Restriction Fragment Length Polymorphism ◽  
Length Polymorphism ◽  
Fragment Length Polymorphism ◽  
Genetic Characterization ◽  
Polymorphism Analysis ◽  
E Coli ◽  
Specific Polysaccharide ◽  
Restriction Fragment Length

ABSTRACT Genetic characterization of the wb* gene in a series ofEscherichia coli and Klebsiella strains possessing the mannose homopolymer as the O-specific polysaccharide was carried out. The partial nucleotide sequences and PCR-restriction fragment length polymorphism analysis suggested that E. coli serotype O9a, a subtype of E. coli O9, might have been generated by the insertion of theKlebsiella O3 wb* gene into a certainE. coli strain.

LYSOGENY IN ENTEROPATHOGENIC ESCHERICHIA COLI: II. CHARACTERIZATION OF E. COLI BACTERIOPHAGE D-1

1961 ◽  
Vol 7 (6) ◽  
pp. 907-913
Author(s):  
John P. Glynn ◽  
W. Robert Bailey
Keyword(s):  
Escherichia Coli ◽  
Host Cell ◽  
Burst Size ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Uv Induction ◽  
Normal Propagation

One of 20 strains of Escherichia coli O111:B4 was found to be lysogenic for a phage which showed activity on several O26:B6 strains. High titers of this phage were obtainable only by UV induction. Normal propagation procedures failed to provide a desirable yield of phage. The calculated burst size of this phage was approximately 36 particles per cell. Other characteristics of the phage, designated D-1 by the authors, are given. Its potentiality as a possible transducing agent was also investigated. Some evidence is offered for the suspected site of attachment on the host cell.

scholarly journals Characterization of typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the classical O55 serogroup by RAPD analysis

1999 ◽  
Vol 30 (4) ◽  
pp. 365-368 ◽  
Author(s):  
Dennys M. Girão ◽  
Sílvia Y. Bando ◽  
Valéria Brígido de C. Girão ◽  
Carlos A. Moreira-Filho ◽  
Sérgio Eduardo L. Fracalanzza ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Epidemiological Studies ◽  
Enteropathogenic Escherichia Coli ◽  
E Coli ◽  
Typical Epec ◽  
Rapd Method ◽  
Atypical Strains

The genetic diversity of 41 typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the serogroup O55 was analyzed by using the random amplified polymorphic DNA (RAPD) method. All typical EPEC O55 strains were grouped in two clusters (A and C) and belonged to the serotype O55:H6, while cluster B included all atypical strains, which were of the serotype O55:H7. The three groups also included non-motile strains. RAPD may be a useful method for epidemiological studies on E. coli O55 infection.

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