scholarly journals TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation

2020 ◽  
Author(s):  
Xiaochen Fan ◽  
V. Pragathi Masamsetti ◽  
Jane Q. J. Sun ◽  
Kasper Engholm-Keller ◽  
Pierre Osteil ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Neural Crest Cell ◽  
Genetic Circuit ◽  
Cellular Functions ◽  
Chromatin Regulators ◽  
Regulatory Module ◽  
Network Propagation ◽  
Neural Crest Differentiation ◽  
Crest Cell

AbstractProtein interaction is critical molecular regulatory activity underlining cellular functions and precise cell fate choices. Using TWIST1 BioID-proximity-labelling and network propagation analyses, we discovered and characterized a TWIST-chromatin regulatory module (TWIST1-CRM) in the neural crest cell (NCC). Combinatorial perturbation of core members of TWIST1-CRM: TWIST1, CHD7, CHD8, and WHSC1 in cell models and mouse embryos revealed that loss of the function of the regulatory module resulted in abnormal specification of NCCs and compromised craniofacial tissue patterning. Our results showed that in the course of cranial neural crest differentiation, phasic activity of TWIST1 and the interacting chromatin regulators promote the choice of NCC fate while suppressing neural stem cell fates, and subsequently enhance ectomesenchyme potential and cell motility. We have revealed the connections between TWIST1 and potential neurocristopathy factors which are functionally interdependent in NCC specification. Moreover, the NCC module participate in the genetic circuit delineating dorsal-ventral patterning of neural progenitors in the neuroepithelium.

scholarly journals TWIST1 and chromatin regulatory proteins interact to guide neural crest cell differentiation

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Xiaochen Fan ◽  
V Pragathi Masamsetti ◽  
Jane QJ Sun ◽  
Kasper Engholm-Keller ◽  
Pierre Osteil ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Neural Crest Cell ◽  
Regulatory Proteins ◽  
Cellular Functions ◽  
Regulatory Module ◽  
Network Propagation ◽  
High Level ◽  
Craniofacial Tissue ◽  
Crest Cell

Protein interaction is critical molecular regulatory activity underlining cellular functions and precise cell fate choices. Using TWIST1 BioID-proximity-labelling and network propagation analyses, we discovered and characterized a TWIST-chromatin regulatory module (TWIST1-CRM) in the neural crest cells (NCC). Combinatorial perturbation of core members of TWIST1-CRM: TWIST1, CHD7, CHD8, and WHSC1 in cell models and mouse embryos revealed that loss of the function of the regulatory module resulted in abnormal differentiation of NCCs and compromised craniofacial tissue patterning. Following NCC delamination, low level of TWIST1-CRM activity is instrumental to stabilize the early NCC signatures and migratory potential by repressing the neural stem cell programs. High level of TWIST1 module activity at later phases commits the cells to the ectomesenchyme. Our study further revealed the functional interdependency of TWIST1 and potential neurocristopathy factors in NCC development.

A role for rhoB in the delamination of neural crest cells from the dorsal neural tube

Development ◽  
1998 ◽  
Vol 125 (24) ◽  
pp. 5055-5067 ◽  
Author(s):  
J.P. Liu ◽  
T.M. Jessell
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Neural Tube ◽  
Neural Crest Cell ◽  
Neural Crest Cells ◽  
Bmp Signaling ◽  
Dorsal Neural Tube ◽  
Rho Family ◽  
Neural Crest Differentiation ◽  
Crest Cell

The differentiation of neural crest cells from progenitors located in the dorsal neural tube appears to involve three sequential steps: the specification of premigratory neural crest cell fate, the delamination of these cells from the neural epithelium and the migration of neural crest cells in the periphery. BMP signaling has been implicated in the specification of neural crest cell fate but the mechanisms that control the emergence of neural crest cells from the neural tube remain poorly understood. To identify molecules that might function at early steps of neural crest differentiation, we performed a PCR-based screen for genes induced by BMPs in chick neural plate cells. We describe the cloning and characterization of one gene obtained from this screen, rhoB, a member of the rho family GTP-binding proteins. rhoB is expressed in the dorsal neural tube and its expression persists transiently in migrating neural crest cells. BMPs induce the neural expression of rhoB but not the more widely expressed rho family member, rhoA. Inhibition of rho activity by C3 exotoxin prevents the delamination of neural crest cells from neural tube explants but has little effect on the initial specification of premigratory neural crest cell fate or on the later migration of neural crest cells. These results suggest that rhoB has a role in the delamination of neural crest cells from the dorsal neural tube.

scholarly journals The Hippo pathway member YAP enhances human neural crest cell fate and migration

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Christopher J. Hindley ◽  
Alexandra Larisa Condurat ◽  
Vishal Menon ◽  
Ria Thomas ◽  
Luis M. Azmitia ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Hippo Pathway ◽  
Neural Crest Cell ◽  
And Migration ◽  
The Hippo Pathway ◽  
Crest Cell

scholarly journals Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate

Cell Reports ◽  
2019 ◽  
Vol 29 (3) ◽  
pp. 603-616.e5
Author(s):  
Hiroyuki N. Arai ◽  
Fuminori Sato ◽  
Takuya Yamamoto ◽  
Knut Woltjen ◽  
Hiroshi Kiyonari ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Neural Crest Cell ◽  
Bmp Signaling ◽  
Cardiac Neural Crest ◽  
Crest Cell

Regulation of cranial neural crest cell fate by Irf6 and Twist1 interaction

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Walid D. Fakhouri ◽  
Jessica Wildgrube Bertol ◽  
Victoria K. Xie ◽  
Shelby Johnston ◽  
Kelsea Hubka ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Neural Crest Cell ◽  
Cranial Neural Crest ◽  
Cranial Neural Crest Cell ◽  
Crest Cell

scholarly journals Blastula stage specification of avian neural crest

2019 ◽  
Author(s):  
Maneeshi S. Prasad ◽  
Eileen Uribe-Querol ◽  
Jonathan Marquez ◽  
Stephanie Vadasz ◽  
Nathan Yardley ◽  
...  
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Neural Crest Cell ◽  
Regional Identity ◽  
Stem Cell Population ◽  
Chick Embryos ◽  
Intermediate Region ◽  
Fate Map ◽  
Blastula Stage ◽  
Crest Cell

AbstractCell fate specification defines the earliest steps towards a distinct cell lineage. Neural crest, a multipotent stem cell population, is thought to be specified from the ectoderm, but its varied contributions defy canons of segregation potential and challenges its embryonic origin. Aiming to resolve this conflict, we have assayed the earliest specification of neural crest using blastula stage chick embryos. Specification assays on isolated chick epiblast explants identify an intermediate region specified towards the neural crest cell fate. Furthermore, low density culture suggests that the specification of intermediate cells towards the neural crest lineage is independent of contact mediated induction. Finally, we have validated the regional identity of the intermediate region towards the neural crest cell fate using fate map studies in blastula stage chick embryos. Our results suggest a model of neural crest specification at blastula stage, with restricted ectoderm and mesoderm capacities.

scholarly journals Neural Crest Cell Fate

2007 ◽  
Vol 1 (4) ◽  
pp. 199-201 ◽  
Author(s):  
Frances Lefcort ◽  
Lynn George
Keyword(s):  
Neural Crest ◽  
Cell Fate ◽  
Neural Crest Cell ◽  
Crest Cell

Composite ganglioneuroma-paraganglioma of the filum terminale

2010 ◽  
Vol 12 (6) ◽  
pp. 709-713 ◽  
Author(s):  
Ganesh M. Shankar ◽  
Li Chen ◽  
Albert H. Kim ◽  
Gina L. Ross ◽  
Rebecca D. Folkerth ◽  
...  
Keyword(s):  
Neural Crest ◽  
Progenitor Cells ◽  
Cell Fate ◽  
Neural Crest Cell ◽  
Immunohistochemical Analysis ◽  
Low Back ◽  
Filum Terminale ◽  
Divergent Differentiation ◽  
History Of ◽  
Crest Cell

Extraadrenal paragangliomas are most commonly found in the carotid body and are also found with lower frequency in the CNS. These lesions are derived from the sympathoadrenal lineage of neural crest cells. Here, the authors report a rare case of a composite paraganglioma with ganglioneuromatous components found at the filum terminale in a patient who presented with a brief history of low-back pain and paresthesias in the inguinal region. Immunohistochemical analysis of the resected lesion revealed admixed elements of neuroendocrine and neuroblastoma lineages, indicating the presence of divergent differentiation of sympathoadrenal progenitor cells. This case represents a unique opportunity to understand the cell fate of sympathoadrenal progenitor cells. Here, the authors propose that paragangliomas at the filum terminale can revert to a neural crest cell precursor fate, giving rise to divergent neoplastic populations.

Sign in / Sign up

Export Citation Format

Share Document