scholarly journals Investigating low-velocity fluid flow in tumours using convection-MRI

2017 ◽  
Author(s):  
Simon Walker-Samuel ◽  
Thomas A. Roberts ◽  
Rajiv Ramasawmy ◽  
Jake Burrell ◽  
S. Peter Johnson ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Interstitial Fluid ◽  
Fluid Pressure ◽  
Interstitial Fluid Pressure ◽  
Tumor Xenograft ◽  
Low Velocity ◽  
Targeting Therapy ◽  
Vascular Flow ◽  
Velocity Flow ◽  
Magnetic Resonance Imaging Mri

AbstractSeveral distinct fluid flow phenemena occur in solid tumours, including intravascular blood flow and interstitial convection. To probe low-velocity flow in tumors resulting from raised interstitial fluid pressure, we have developed a novel magnetic resonance imaging (MRI) technique named convection-MRI. It uses a phase-contrast acquisition with a dual-inversion vascular nulling preparation to separate intra- and extra-vascular flow. Here, we report the results of experiments in flow phantoms, numerical simulations and tumor xenograft models to investigate the technical feasibility of convection-MRI. We report a good correlation between estimates of effective fluid pressure from convection-MRI with gold-standard, invasive measurements of interstitial fluid pressure in mouse models of human colorectal carcinoma and show that convection-MRI can provide insights into the growth and response to vascular-targeting therapy in colorectal cancers.

Interstitial fluid pressure gradient measured by micropuncture in excised dog lung

1984 ◽  
Vol 56 (2) ◽  
pp. 271-277 ◽  
Author(s):  
J. Bhattacharya ◽  
M. A. Gropper ◽  
N. C. Staub
Keyword(s):  
Fluid Flow ◽  
Pressure Gradient ◽  
Pressure Difference ◽  
Airway Pressure ◽  
Interstitial Fluid ◽  
Fluid Pressure ◽  
Interstitial Fluid Pressure ◽  
Pleural Pressure ◽  
Alveolar Wall ◽  
Fluid Filtration

We have directly measured lung interstitial fluid pressure at sites of fluid filtration by micropuncturing excised left lower lobes of dog lung. We blood-perfused each lobe after cannulating its artery, vein, and bronchus to produce a desired amount of edema. Then, to stop further edema, we air-embolized the lobe. Holding the lobe at a constant airway pressure of 5 cmH2O, we measured interstitial fluid pressure using beveled glass micropipettes and the servo-null method. In 31 lobes, divided into 6 groups according to severity of edema, we micropunctured the subpleural interstitium in alveolar wall junctions, in adventitia around 50-micron venules, and in the hilum. In all groups an interstitial fluid pressure gradient existed from the junctions to the hilum. Junctional, adventitial, and hilar pressures, which were (relative to pleural pressure) 1.3 +/- 0.2, 0.3 +/- 0.5, and -1.8 +/- 0.2 cmH2O, respectively, in nonedematous lobes, rose with edema to plateau at 4.1 +/- 0.4, 2.0 +/- 0.2, and 0.4 +/- 0.3 cmH2O, respectively. We also measured junctional and adventitial pressures near the base and apex in each of 10 lobes. The pressures were identical, indicating no vertical interstitial fluid pressure gradient in uniformly expanded nonedematous lobes which lack a vertical pleural pressure gradient. In edematous lobes basal pressure exceeded apical but the pressure difference was entirely attributable to greater basal edema. We conclude that the presence of an alveolohilar gradient of lung interstitial fluid pressure, without a base-apex gradient, represents the mechanism for driving fluid flow from alveoli toward the hilum.

Control of interstitial fluid pressure: Role of [beta ]-integrins

2001 ◽  
Vol 21 (3) ◽  
pp. 222-230 ◽  
Author(s):  
Rolf K. Reed ◽  
Ansgar Berg ◽  
Eli-Anne B. Gjerde ◽  
Kristofer Rubin
Keyword(s):  
Interstitial Fluid ◽  
Fluid Pressure ◽  
Interstitial Fluid Pressure

Dynamics of Interstitial Fluid Pressure in Extracellular Matrix Hydrogels in Microfluidic Devices

2015 ◽  
Vol 137 (9) ◽  
Author(s):  
Joe Tien ◽  
Le Li ◽  
Ozgur Ozsun ◽  
Kamil L. Ekinci
Keyword(s):  
Extracellular Matrix ◽  
Pressure Distribution ◽  
Interstitial Fluid ◽  
Scaling Laws ◽  
Fluid Pressure ◽  
Interstitial Fluid Pressure ◽  
External Loading ◽  
Interstitial Pressure ◽  
Time Resolved ◽  
Long Time

In order to understand how interstitial fluid pressure and flow affect cell behavior, many studies use microfluidic approaches to apply externally controlled pressures to the boundary of a cell-containing gel. It is generally assumed that the resulting interstitial pressure distribution quickly reaches a steady-state, but this assumption has not been rigorously tested. Here, we demonstrate experimentally and computationally that the interstitial fluid pressure within an extracellular matrix gel in a microfluidic device can, in some cases, react with a long time delay to external loading. Remarkably, the source of this delay is the slight (∼100 nm in the cases examined here) distension of the walls of the device under pressure. Finite-element models show that the dynamics of interstitial pressure can be described as an instantaneous jump, followed by axial and transverse diffusion, until the steady pressure distribution is reached. The dynamics follow scaling laws that enable estimation of a gel's poroelastic constants from time-resolved measurements of interstitial fluid pressure.

Simultaneous Measurement of Interstitial Fluid Pressure and Load in Rat Skin After Strain Application In Vitro

2003 ◽  
Vol 31 (10) ◽  
pp. 1246-1254 ◽  
Author(s):  
David M. Wright ◽  
Helge Wiig ◽  
C. Peter Winlove ◽  
Joel L. Bert ◽  
Rolf K. Reed
Keyword(s):  
Simultaneous Measurement ◽  
Interstitial Fluid ◽  
Fluid Pressure ◽  
Interstitial Fluid Pressure ◽  
Rat Skin

scholarly journals Combined Anti-Angiogenic Therapy Targeting PDGF and VEGF Receptors Lowers the Interstitial Fluid Pressure in a Murine Experimental Carcinoma

PLoS ONE ◽  
2009 ◽  
Vol 4 (12) ◽  
pp. e8149 ◽  
Author(s):  
Agnieszka Kłosowska-Wardęga ◽  
Yoko Hasumi ◽  
Mikhail Burmakin ◽  
Aive Åhgren ◽  
Linda Stuhr ◽  
...  
Keyword(s):  
Interstitial Fluid ◽  
Fluid Pressure ◽  
Interstitial Fluid Pressure ◽  
Vegf Receptors ◽  
Angiogenic Therapy ◽  
Experimental Carcinoma
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