scholarly journals Anaerobic methanotrophic communities thrive in deep submarine permafrost

2017 ◽  
Author(s):  
Matthias Winkel ◽  
Julia Mitzscherling ◽  
Pier P. Overduin ◽  
Fabian Horn ◽  
Maria Winterfeld ◽  
...  
Keyword(s):  
Low Temperatures ◽  
Marker Genes ◽  
Anaerobic Oxidation Of Methane ◽  
Oxidation Of Methane ◽  
Iron Manganese ◽  
Archaeal Communities ◽  
Permafrost Sediments ◽  
Methane Concentrations ◽  
Methanotrophic Communities

AbstractThawing submarine permafrost is a source of methane to the subsurface biosphere. Methane oxidation in submarine permafrost sediments has been proposed, but the responsible microorganisms remain uncharacterized. We analyzed archaeal communities and identified distinct anaerobic methanotrophic (ANME-2a/b, ANME-2d) assemblages in frozen and completely thawed submarine permafrost sediments. Besides archaea potentially involved in AOM we found a large diversity of archaea mainly belonging to Bathyarchaeota, Thaumarchaeota, and Euryarchaeota. Methane concentrations and δ13C-methane signatures distinguish horizons of potential anaerobic oxidation of methane (AOM) coupled either to sulfate reduction in a sulfate-methane transition zone (SMTZ) or to the reduction of other electron acceptors, such as iron, manganese or nitrate. Analysis of functional marker genes (mcrA) and fluorescence in situ hybridization (FISH) corroborate AOM communities in submarine permafrost sediments potentially active at low temperatures. Extrapolating potential AOM rates, when scaled to the total area of expected submarine permafrost thaw, reveals that methane could be consumed at rates between 8 and 120 Tg C per year, which is comparable to other AOM habitats such as seeps, continental SMTZ and wetlands. We thus propose that AOM is active where submarine permafrost thaws and needs to be accounted for in global methane budgets.

scholarly journals Simultaneous Nitrite-Dependent Anaerobic Methane and Ammonium Oxidation Processes

2011 ◽  
Vol 77 (19) ◽  
pp. 6802-6807 ◽  
Author(s):  
Francisca A. Luesken ◽  
Jaime Sánchez ◽  
Theo A. van Alen ◽  
Janeth Sanabria ◽  
Huub J. M. Op den Camp ◽  
...  
Keyword(s):  
Enrichment Culture ◽  
Anammox Bacteria ◽  
Anaerobic Oxidation Of Methane ◽  
Ammonium Oxidation ◽  
Content Type ◽  
Oxidation Of Methane ◽  
Activity Measurements ◽  
Near Future ◽  
Damo Bacteria

ABSTRACTNitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that are catalyzed by n-damo bacteria, including “CandidatusMethylomirabilis oxyfera,” and anammox bacteria, respectively. The feasibility of coculturing anammox and n-damo bacteria is important for implementation in wastewater treatment systems that contain substantial amounts of both methane and ammonium. Here we tested this possible coexistence experimentally. To obtain such a coculture, ammonium was fed to a stable enrichment culture of n-damo bacteria that still contained some residual anammox bacteria. The ammonium supplied to the reactor was consumed rapidly and could be gradually increased from 1 to 20 mM/day. The enriched coculture was monitored by fluorescencein situhybridization and 16S rRNA andpmoAgene clone libraries and activity measurements. After 161 days, a coculture with about equal amounts of n-damo and anammox bacteria was established that converted nitrite at a rate of 0.1 kg-N/m3/day (17.2 mmol day−1). This indicated that the application of such a coculture for nitrogen removal may be feasible in the near future.

scholarly journals Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

2010 ◽  
Vol 7 (5) ◽  
pp. 7945-7983 ◽  
Author(s):  
K. A. Smemo ◽  
J. B. Yavitt
Keyword(s):  
Large Body ◽  
Conclusive Evidence ◽  
Future Research ◽  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
Ch4 Oxidation ◽  
Oxidation Of Methane ◽  
Ch4 Production

Abstract. Despite a large body of literature on microbial anaerobic oxidation of methane (AOM) in marine sediments and saline waters and its importance to the global methane (CH4) cycle, until recently little work has addressed the potential occurrence and importance of AOM in non-marine systems. This is particularly true for peatlands, which represent both a massive sink for atmospheric CO2 and a significant source of atmospheric CH4. Our knowledge of this process in peatlands is inherently limited by the methods used to study CH4 dynamics in soil and sediment and the assumption that there are no anaerobic sinks for CH4 in these systems. Studies suggest that AOM is CH4-limited and difficult to detect in potential CH4 production assays against a background of CH4 production. In situ rates also might be elusive due to background rates of aerobic CH4 oxidation and the difficulty in separating net and gross process rates. Conclusive evidence for the electron acceptor in this process has not been presented. Nitrate and sulfate are both plausible and favorable electron acceptors, as seen in other systems, but there exist theoretical issues related to the availability of these ions in peatlands and only circumstantial evidence suggests that these pathways are important. Iron cycling is important in many wetland systems, but recent evidence does not support the notion of CH4 oxidation via dissimilatory Fe(III) reduction or a CH4 oxidizing archaea in consortium with an Fe(III) reducer. Calculations based on published rates demonstrate that AOM might be a significant and underappreciated constraint on the global CH4 cycle, although much about the process in unknown, in vitro rates may not relate well to in situ rates, and projections based on those rates are fraught with uncertainty. We suggest electron transfer mechanisms, C flow and pathways, and quantifying in situ peatland AOM rates as the highest priority topics for future research.

scholarly journals Anaerobic Oxidization of Methane in a Minerotrophic Peatland: Enrichment of Nitrite-Dependent Methane-Oxidizing Bacteria

2012 ◽  
Vol 78 (24) ◽  
pp. 8657-8665 ◽  
Author(s):  
Baoli Zhu ◽  
Gijs van Dijk ◽  
Christian Fritz ◽  
Alfons J. P. Smolders ◽  
Arjan Pol ◽  
...  
Keyword(s):  
16S Rrna ◽  
Transition Zone ◽  
Anaerobic Oxidation Of Methane ◽  
Potential Contribution ◽  
Content Type ◽  
Cell Numbers ◽  
Oxidation Of Methane ◽  
Methane Oxidizing Bacteria ◽  
Minerotrophic Peatland

ABSTRACTThe importance of anaerobic oxidation of methane (AOM) as a methane sink in freshwater systems is largely unexplored, particularly in peat ecosystems. Nitrite-dependent anaerobic methane oxidation (n-damo) was recently discovered and reported to be catalyzed by the bacterium “CandidatusMethylomirabilis oxyfera,” which is affiliated with the NC10 phylum. So far, several “Ca. Methylomirabilis oxyfera” enrichment cultures have been obtained using a limited number of freshwater sediments or wastewater treatment sludge as the inoculum. In this study, using stable isotope measurements and porewater profiles, we investigated the potential of n-damo in a minerotrophic peatland in the south of the Netherlands that is infiltrated by nitrate-rich ground water. Methane and nitrate profiles suggested that all methane produced was oxidized before reaching the oxic layer, and NC10 bacteria could be active in the transition zone where countergradients of methane and nitrate occur. Quantitative PCR showed high NC10 bacterial cell numbers at this methane-nitrate transition zone. This soil section was used to enrich the prevalent NC10 bacteria in a continuous culture supplied with methane and nitrite at anin situpH of 6.2. An enrichment of nitrite-reducing methanotrophic NC10 bacteria was successfully obtained. Phylogenetic analysis of retrieved 16S rRNA andpmoAgenes showed that the enriched bacteria were very similar to the ones foundin situand constituted a new branch of NC10 bacteria with an identity of less than 96 and 90% to the 16S rRNA andpmoAgenes of “Ca. Methylomirabilis oxyfera,” respectively. The results of this study expand our knowledge of the diversity and distribution of NC10 bacteria in the environment and highlight their potential contribution to nitrogen and methane cycles.

scholarly journals Electron Acceptor Availability Shapes Anaerobically Methane Oxidizing Archaea (ANME) Communities in South Georgia Sediments

2021 ◽  
Vol 12 ◽  
Author(s):  
Annika Schnakenberg ◽  
David A. Aromokeye ◽  
Ajinkya Kulkarni ◽  
Lisa Maier ◽  
Lea C. Wunder ◽  
...  
Keyword(s):  
Community Composition ◽  
Electron Acceptor ◽  
Electron Acceptors ◽  
Anaerobic Oxidation Of Methane ◽  
Atmospheric Methane ◽  
Environmental Filters ◽  
South Georgia ◽  
Oxidation Of Methane ◽  
High Concentrations

Anaerobic methane oxidizing archaea (ANME) mediate anaerobic oxidation of methane (AOM) in marine sediments and are therefore important for controlling atmospheric methane concentrations in the water column and ultimately the atmosphere. Numerous previous studies have revealed that AOM is coupled to the reduction of different electron acceptors such as sulfate, nitrate/nitrite or Fe(III)/Mn(IV). However, the influence of electron acceptor availability on the in situ ANME community composition in sediments remains largely unknown. Here, we investigated the electron acceptor availability and compared the microbial in situ communities of three methane-rich locations offshore the sub-Antarctic island South Georgia, by Illumina sequencing and qPCR of mcrA genes. The methanic zone (MZ) sediments of Royal Trough and Church Trough comprised high sulfide concentrations of up to 4 and 19 mM, respectively. In contrast, those of the Cumberland Bay fjord accounted for relatively high concentrations of dissolved iron (up to 186 μM). Whereas the ANME community in the sulfidic sites Church Trough and Royal Trough mainly comprised members of the ANME-1 clade, the order-level clade “ANME-1-related” (Lever and Teske, 2015) was most abundant in the iron-rich site in Cumberland Bay fjord, indicating that the availability of electron acceptors has a strong selective effect on the ANME community. This study shows that potential electron acceptors for methane oxidation may serve as environmental filters to select for the ANME community composition and adds to a better understanding of the global importance of AOM.

scholarly journals Visualizing in situ translational activity for identifying and sorting slow-growing archaeal−bacterial consortia

2016 ◽  
Vol 113 (28) ◽  
pp. E4069-E4078 ◽  
Author(s):  
Roland Hatzenpichler ◽  
Stephanie A. Connon ◽  
Danielle Goudeau ◽  
Rex R. Malmstrom ◽  
Tanja Woyke ◽  
...  
Keyword(s):  
16S Rrna Gene Sequencing ◽  
Microbial Consortia ◽  
Rrna Gene ◽  
Anaerobic Oxidation Of Methane ◽  
Bacterial Consortia ◽  
Symbiotic Relationships ◽  
Oxidation Of Methane ◽  
Translational Activity ◽  
Rrna Gene Sequencing

To understand the biogeochemical roles of microorganisms in the environment, it is important to determine when and under which conditions they are metabolically active. Bioorthogonal noncanonical amino acid tagging (BONCAT) can reveal active cells by tracking the incorporation of synthetic amino acids into newly synthesized proteins. The phylogenetic identity of translationally active cells can be determined by combining BONCAT with rRNA-targeted fluorescence in situ hybridization (BONCAT-FISH). In theory, BONCAT-labeled cells could be isolated with fluorescence-activated cell sorting (BONCAT-FACS) for subsequent genetic analyses. Here, in the first application, to our knowledge, of BONCAT-FISH and BONCAT-FACS within an environmental context, we probe the translational activity of microbial consortia catalyzing the anaerobic oxidation of methane (AOM), a dominant sink of methane in the ocean. These consortia, which typically are composed of anaerobic methane-oxidizing archaea (ANME) and sulfate-reducing bacteria, have been difficult to study due to their slow in situ growth rates, and fundamental questions remain about their ecology and diversity of interactions occurring between ANME and associated partners. Our activity-correlated analyses of >16,400 microbial aggregates provide the first evidence, to our knowledge, that AOM consortia affiliated with all five major ANME clades are concurrently active under controlled conditions. Surprisingly, sorting of individual BONCAT-labeled consortia followed by whole-genome amplification and 16S rRNA gene sequencing revealed previously unrecognized interactions of ANME with members of the poorly understood phylum Verrucomicrobia. This finding, together with our observation that ANME-associated Verrucomicrobia are found in a variety of geographically distinct methane seep environments, suggests a broader range of symbiotic relationships within AOM consortia than previously thought.

In Situ X-ray Photoelectron Spectroscopy Detects Multiple Active Sites Involved in the Selective Anaerobic Oxidation of Methane in Copper-Exchanged Zeolites

ACS Catalysis ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 6728-6737 ◽  
Author(s):  
Luca Artiglia ◽  
Vitaly L. Sushkevich ◽  
Dennis Palagin ◽  
Amy J. Knorpp ◽  
Kanak Roy ◽  
...  
Keyword(s):  
Photoelectron Spectroscopy ◽  
Active Sites ◽  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
X Ray ◽  
Oxidation Of Methane

scholarly journals Biogeochemical evidence of anaerobic methane oxidation on active submarine mud volcanoes on the continental slope of the Canadian Beaufort Sea

2018 ◽  
Vol 15 (24) ◽  
pp. 7419-7433 ◽  
Author(s):  
Dong-Hun Lee ◽  
Jung-Hyun Kim ◽  
Yung Mi Lee ◽  
Alina Stadnitskaia ◽  
Young Keun Jin ◽  
...  
Keyword(s):  
Continental Slope ◽  
Distribution Patterns ◽  
Beaufort Sea ◽  
Anaerobic Oxidation Of Methane ◽  
Mud Volcanoes ◽  
Operational Taxonomic Units ◽  
Carbon Isotopic ◽  
Oxidation Of Methane ◽  
Lipid Biomarker ◽  
Archaeal Communities

Abstract. In this study, we report lipid biomarker patterns and phylogenetic identities of key microbial communities mediating anaerobic oxidation of methane (AOM) in active mud volcanoes (MVs) on the continental slope of the Canadian Beaufort Sea. The carbon isotopic compositions (δ13C) of sn-2- and sn-3-hydroxyarchaeol showed the highly 13C-depleted values (−114 ‰ to −82 ‰) associated with a steep depletion in sulfate concentrations within 0.7 m of sediment depths. This suggested the presence of methanotrophic archaea involved in sulfate-dependent AOM, albeit in a small amount. The ratio of sn-2-hydroxyarchaeol to archaeol (> 1) and operational taxonomic units (OTUs) indicated that the anaerobic methanotrophic archaea (ANME) clades ANME-2c and ANME-3 were involved in AOM. Higher δ13C values of archaeol and biphytanes (BPs; -55.2±10.0 ‰ and -39.3±13.0 ‰, respectively) suggested that archaeal communities were also assimilating AOM-derived inorganic carbon. Furthermore, the distinct distribution patterns of methanotrophs in the three MVs appears to be associated with varying intensities of ascending gas fluids. Consequently, our results suggest that the niche diversification of active mud volcanoes has shaped distinct archaeal communities that play important roles in AOM in the Beaufort Sea.

scholarly journals Laboratory Experimental Study on the Formation of Authigenic Carbonates Induced by Microbes in Marine Sediments

2021 ◽  
Vol 9 (5) ◽  
pp. 479
Author(s):  
Zilin Wei ◽  
Tianfu Xu ◽  
Songhua Shang ◽  
Hailong Tian ◽  
Yuqing Cao ◽  
...  
Keyword(s):  
Marine Sediments ◽  
Water Environment ◽  
Anaerobic Oxidation Of Methane ◽  
Carbonate Mineral ◽  
Authigenic Carbonates ◽  
Preferential Formation ◽  
Oxidation Of Methane ◽  
Long Time ◽  
Three Stages

Authigenic carbonates are widely distributed in marine sediments, microbes, and anaerobic oxidation of methane (AOM) play a key role in their formation. The authigenic carbonates in marine sediments have been affected by weathering and diagenesis for a long time, it is difficult to understand their formation process by analyzing the samples collected in situ. A pore water environment with 10 °C, 6 MPa in the marine sediments was built in a bioreactor to study the stages and characteristics of authigenic carbonates formation induced by microbes. In experiments, FeCO3 is formed preferentially, and then FeCO3-MgCO3 complete isomorphous series and a small part of CaCO3 isomorphous mixture are formed. According to this, it is proposed that the formation of authigenic carbonates performed by AOM and related microbes needs to undergo three stages: the rise of alkalinity, the preferential formation of a carbonate mineral, and the formation of carbonate isomorphous series. This work provides experimental experience and reference basis for further understanding the formation mechanism of authigenic carbonates in marine sediments.

scholarly journals Biogeochemical and microbiological evidence for methane-related archaeal communities at active submarine mud volcanoes on the Canadian Beaufort Sea slope

2018 ◽  
Author(s):  
Dong-Hun Lee ◽  
Jung-Hyun Kim ◽  
Yung Mi Lee ◽  
Alina Stadnitskaia ◽  
Young Keun Jin ◽  
...  
Keyword(s):  
16S Rrna ◽  
Surface Sediments ◽  
Beaufort Sea ◽  
Lipid Biomarkers ◽  
Anaerobic Oxidation Of Methane ◽  
Mud Volcanoes ◽  
Δ13c Values ◽  
Oxidation Of Methane ◽  
Lipid Biomarker ◽  
Archaeal Communities

Abstract. In this study, we report lipid biomarker patterns and phylogenetic identities of key microbes mediating anaerobic oxidation of methane (AOM) communities in active mud volcanos (MVs) on the continental slope of the Canadian Beaufort Sea. The enriched δ13C values of total organic carbon (TOC) as well as lipid biomarkers such as archaeol and biphytanes (BPs) relative to δ13CCH4 values suggested that the contribution of AOM-related biomass to sedimentary TOC was in general negligible in the Beaufort Sea MVs investigated. However, the δ13C values of sn-2- and sn-3-hydroxyarchaeol were more negative than CH4, indicating the presence of AOM communities, albeit in a small amount. The ratio of sn-2-hydroxyarchaeol to archaeol and the 16S rRNA results indeed indicated that archaea of the ANME-2c and ANME-3 clades were involved in AOM. Further studies are needed to investigate the diversity and distribution of AOM communities and to characterize their habitats in the uppermost surface sediments of Beaufort Sea MV systems.

scholarly journals Anaerobic oxidation of methane: an underappreciated aspect of methane cycling in peatland ecosystems?

2011 ◽  
Vol 8 (3) ◽  
pp. 779-793 ◽  
Author(s):  
K. A. Smemo ◽  
J. B. Yavitt
Keyword(s):  
Large Body ◽  
Conclusive Evidence ◽  
Future Research ◽  
Anaerobic Oxidation Of Methane ◽  
Anaerobic Oxidation ◽  
Ch4 Oxidation ◽  
Oxidation Of Methane ◽  
Ch4 Production

Abstract. Despite a large body of literature on microbial anaerobic oxidation of methane (AOM) in marine sediments and saline waters and its importance to the global methane (CH4) cycle, until recently little work has addressed the potential occurrence and importance of AOM in non-marine systems. This is particularly true for peatlands, which represent both a massive sink for atmospheric CO2 and a significant source of atmospheric CH4. Our knowledge of this process in peatlands is inherently limited by the methods used to study CH4 dynamics in soil and sediment and the assumption that there are no anaerobic sinks for CH4 in these systems. Studies suggest that AOM is CH4-limited and difficult to detect in potential CH4 production assays against a background of CH4 production. In situ rates also might be elusive due to background rates of aerobic CH4 oxidation and the difficulty in separating net and gross process rates. Conclusive evidence for the electron acceptor in this process has not been presented. Nitrate and sulfate are both plausible and favorable electron acceptors, as seen in other systems, but there exist theoretical issues related to the availability of these ions in peatlands and only circumstantial evidence suggests that these pathways are important. Iron cycling is important in many wetland systems, but recent evidence does not support the notion of CH4 oxidation via dissimilatory Fe(III) reduction or a CH4 oxidizing archaea in consortium with an Fe(III) reducer. Calculations based on published rates demonstrate that AOM might be a significant and underappreciated constraint on the global CH4 cycle, although much about the process is unknown, in vitro rates may not relate well to in situ rates, and projections based on those rates are fraught with uncertainty. We suggest electron transfer mechanisms, C flow and pathways, and quantifying in situ peatland AOM rates as the highest priority topics for future research.

Sign in / Sign up

Export Citation Format

Share Document