scholarly journals Optimization of Alginante-poly-L-lysine Microencapsules Strength by Box-Behnken Model for CHO cells Culture in Stirred Tank Bioreactor

2017 ◽  
Author(s):  
Yu Wang ◽  
Na Li ◽  
Dongsheng Sun ◽  
Shengnan He ◽  
Lisha Mou
Keyword(s):  
Cell Culture ◽  
Cho Cells ◽  
Protein Production ◽  
Maximum Strength ◽  
Stirred Tank ◽  
Process Conditions ◽  
Membrane Thickness ◽  
Reaction Conditions ◽  
Stirred Tank Bioreactor

Summary statementBox-Behnken model is an efficient method to optimized process conditions of microencapsules strength for long-term cell culture, promotes CHO cells viability and protein production in a stirred tank bioreactor.ABSTRACTCell microencapsulation technology has been proved to be a valuable technology in the fields of large-scale cell culture. It is important to accurately construct the microcapsule membranes with desired properties including a certain thickness suitable for cell growth, and maximum strength for the stability of microencapsules. As single factor experiments are time-consuming to obtain the desired membrane preparation conditions, Box-Behnken model was used to investigate the interactions among reaction conditions, predict the optimized reaction conditions for given purpose, that was membrane with maximum strength and desired thickness for microencapsulated cell culture. Significant values of R2 in this study indicated the model theoretical values are very close to the measured values. Based on the desired membrane thickness, the process of maximum strength was optimized, and the prediction agreement of measured value and model theoretical value was 91.11%. The optimized microencapsules with maximum strength and 15 μm membrane thickness promote CHO cells viability and protein production in stirred tank bioreactor. The result shows that Box-Behnken model is an efficient method to optimized process conditions of microencapsules strength for long-term cell culture.

scholarly journals Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Azmir Arifin ◽  
Maizirwan Mel ◽  
Mohamed Ismail Abdul Karim ◽  
Aini Ideris
Keyword(s):  
Cell Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
High Speed ◽  
Virus Titer ◽  
Vero Cells ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Maximum Cell

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.

The influence of dissolved CO₂ concentration and gas bubbles on CHO cells growth and metabolism

2021 ◽  
pp. 22-26
Author(s):  
Vera Vyacheslavovna Eliseeva ◽  
Aleksandr Alekseevich Smirnov ◽  
Denis Alekseevich Mazalyov ◽  
Viktor Andreevich Kuvshinov
Keyword(s):  
Carbon Dioxide ◽  
Cell Culture ◽  
Recombinant Proteins ◽  
Cho Cells ◽  
Protein Production ◽  
Chinese Hamster ◽  
Culture Conditions ◽  
Co Concentration ◽  
Cell Culture Conditions ◽  
Cells Cultured

Chinese hamster ovary (CHO) cells cultured in bioreactors to produce various recombinant proteins for therapeutic applications. The concentration of carbon dioxide dissolved in the medium is one of the key components affecting the growth and productivity of CHO cells. Researching the effect of different concentrations рCO₂ on cells is necessary for optimization of cell culture conditions and increasing protein production. The review deals with research in this field.

Large-scale propagation of a replication-defective adenovirus vector in stirred-tank bioreactor PER.C6? cell culture under sparging conditions

2003 ◽  
Vol 83 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Liangzhi Xie ◽  
Christian Metallo ◽  
James Warren ◽  
Warren Pilbrough ◽  
Joseph Peltier ◽  
...  
Keyword(s):  
Cell Culture ◽  
Large Scale ◽  
Adenovirus Vector ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Replication Defective Adenovirus

CHARACTERISTICS OF POST-FLIGHT MUSCLE STRENGTH AND VELOCITY DYNAMICS IN COSMONAUTS AS A FUNCTION OF WEIGHT LOADING DURING LONG-TERM SPACE MISSIONS

2020 ◽  
Vol 54 (5) ◽  
pp. 23-28
Author(s):  
E.V. Fomina ◽  
◽  
T.B. Kukoba ◽  
Keyword(s):  
Muscle Strength ◽  
Body Mass ◽  
Flight Muscle ◽  
Space Mission ◽  
The Body ◽  
Maximum Strength ◽  
Training Device ◽  
Leg Muscle ◽  
Weight Loading

Testing of 25 cosmonauts showed that the amount of resistance training weight loading in long-term space mission influences dynamics of the leg-muscle strength and velocity recovery. On Earth, the loads equal from 70 to 130 % of the body mass is sufficient for keeping up endurance and maximum strength moments of shin and thigh muscles. In the group of cosmonauts who had not used the strength training device or chosen loads less than 30 % of the body mass the leg-muscle maximum strength and thigh endurance were decreased substantially on day 4 of return and all the more by day 15 back on Earth.

Amperometric assay of vitamin C using an ascorbate oxidase enzyme electrode

1979 ◽  
Vol 44 (11) ◽  
pp. 3395-3404 ◽  
Author(s):  
Pavel Posádka ◽  
Lumír Macholán
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Oxygen Electrode ◽  
Ascorbate Oxidase ◽  
Current Response ◽  
Reaction Conditions ◽  
Long Term Storage ◽  
Oxidase Enzyme ◽  
Optimal Reaction

An oxygen electrode of the Clark type, coated by a thin, active layer of chemically insolubilized ascorbate oxidase from squash peelings specifically detects by measuring oxygen uptake 10 to 400 μg of ascorbic acid in 3 ml of phosphate buffer. The record of current response to substrate addition lasts 1-2 min. The ascorbic acid values determined in various samples of fruit juices are in good agreement with the data obtained by titration and polarography. The suitable composition of the membrane and its lifetime and stability during long-term storage are described; optimal reaction conditions of vitamin C determination and the possibilities of interference of other compounds are also examined. Of the 35 phenols, aromatic amines and acids tested chlorogenic acid only can cause a positive error provided that the enzyme membrane has been prepared from ascorbate oxidase of high purity.

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