Rapid optimization of process conditions for cultivation of transgenic Laminaria japonica gametophyte cells in a stirred-tank bioreactor

2006 ◽  
Vol 122 (1-2) ◽  
pp. 11-14 ◽  
Author(s):  
Jiangtao Gao ◽  
Song Qin ◽  
Yicheng Zhang
Keyword(s):  
Laminaria Japonica ◽  
Stirred Tank ◽  
Process Conditions ◽  
Stirred Tank Bioreactor

scholarly journals Optimization of Alginante-poly-L-lysine Microencapsules Strength by Box-Behnken Model for CHO cells Culture in Stirred Tank Bioreactor

2017 ◽  
Author(s):  
Yu Wang ◽  
Na Li ◽  
Dongsheng Sun ◽  
Shengnan He ◽  
Lisha Mou
Keyword(s):  
Cell Culture ◽  
Cho Cells ◽  
Protein Production ◽  
Maximum Strength ◽  
Stirred Tank ◽  
Process Conditions ◽  
Membrane Thickness ◽  
Reaction Conditions ◽  
Stirred Tank Bioreactor

Summary statementBox-Behnken model is an efficient method to optimized process conditions of microencapsules strength for long-term cell culture, promotes CHO cells viability and protein production in a stirred tank bioreactor.ABSTRACTCell microencapsulation technology has been proved to be a valuable technology in the fields of large-scale cell culture. It is important to accurately construct the microcapsule membranes with desired properties including a certain thickness suitable for cell growth, and maximum strength for the stability of microencapsules. As single factor experiments are time-consuming to obtain the desired membrane preparation conditions, Box-Behnken model was used to investigate the interactions among reaction conditions, predict the optimized reaction conditions for given purpose, that was membrane with maximum strength and desired thickness for microencapsulated cell culture. Significant values of R2 in this study indicated the model theoretical values are very close to the measured values. Based on the desired membrane thickness, the process of maximum strength was optimized, and the prediction agreement of measured value and model theoretical value was 91.11%. The optimized microencapsules with maximum strength and 15 μm membrane thickness promote CHO cells viability and protein production in stirred tank bioreactor. The result shows that Box-Behnken model is an efficient method to optimized process conditions of microencapsules strength for long-term cell culture.

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

Utilisation of winery wastewater for xanthan production in stirred tank bioreactor: Bioprocess modelling and optimisation

2019 ◽  
Vol 117 ◽  
pp. 113-125 ◽  
Author(s):  
Zorana Rončević ◽  
Jovana Grahovac ◽  
Siniša Dodić ◽  
Damjan Vučurović ◽  
Jelena Dodić
Keyword(s):  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Winery Wastewater ◽  
Xanthan Production

scholarly journals Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Mohd Azmir Arifin ◽  
Maizirwan Mel ◽  
Mohamed Ismail Abdul Karim ◽  
Aini Ideris
Keyword(s):  
Cell Culture ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
High Speed ◽  
Virus Titer ◽  
Vero Cells ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Maximum Cell

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.

Monoterpenoid Oxindole Alkaloid Production by Uncaria tomentosa (Willd) D.C. Cell Suspension Cultures in a Stirred Tank Bioreactor

2008 ◽  
Vol 21 (3) ◽  
pp. 786-792 ◽  
Author(s):  
Gabriela Trejo-Tapia ◽  
Carlos M. Cerda-García-Rojas ◽  
Mario Rodríguez-Monroy ◽  
Ana C. Ramos-Valdivia
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Stirred Tank ◽  
Alkaloid Production ◽  
Stirred Tank Bioreactor ◽  
Uncaria Tomentosa

Biosurfactant production by Aureobasidium pullulans in stirred tank bioreactor: New approach to understand the influence of important variables in the process

2017 ◽  
Vol 243 ◽  
pp. 264-272 ◽  
Author(s):  
Larissa Pereira Brumano ◽  
Felipe Antonio Fernandes Antunes ◽  
Sara Galeno Souto ◽  
Júlio Cesar dos Santos ◽  
Joachim Venus ◽  
...  
Keyword(s):  
Aureobasidium Pullulans ◽  
Biosurfactant Production ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
New Approach
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