scholarly journals High-NA open-top selective-plane illumination microscopy for biological imaging

2017 ◽  
Author(s):  
Ryan McGorty ◽  
Dan Xie ◽  
Bo Huang
Keyword(s):  
Adaptive Optics ◽  
Biological Sample ◽  
Spatial Configuration ◽  
Numerical Aperture ◽  
Biological Imaging ◽  
Imaging Systems ◽  
Optical Components ◽  
Volumetric Imaging ◽  
Selective Plane Illumination Microscopy ◽  
Living Organisms

Abstract:Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for volumetric imaging of living organisms over extended times. However, the spatial configuration of a SPIM system often limits its compatibility with many widely used biological sample holders such as multi-well chambers and plates. To solve this problem, we developed a high numerical aperture (NA) open-top configuration that places both the excitation and detection objectives on the opposite of the sample coverglass. We carried out a theoretical calculation to analyze the structure of the system-induced aberrations. We then experimentally compensated the system aberrations using adaptive optics combined with static optical components, demonstrating near-diffraction-limited performance in imaging fluorescently labeled cells.© 2017 Optical Society of AmericaOCIS codes: (080.080) Geometric Optics; (110.0110) Imaging systems; (110.0180) Microscopy.

scholarly journals High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy

2018 ◽  
Author(s):  
Bin Yang ◽  
Yina Wang ◽  
Siyu Feng ◽  
Veronica Pessino ◽  
Nico Stuurman ◽  
...  
Keyword(s):  
Single Molecule ◽  
Numerical Aperture ◽  
Super Resolution ◽  
Live Cells ◽  
Volumetric Imaging ◽  
High Numerical Aperture ◽  
Selective Plane Illumination Microscopy ◽  
Resolution Imaging ◽  
Living Organisms ◽  
Super Resolution Microscopy

Selective-plane illumination microscopy (SPIM) provides unparalleled advantages for long-term volumetric imaging of living organisms. In order to achieve high-resolution imaging in common biological sample holders, we designed a high numerical aperture (NA) epi-illumination SPIM (eSPIM) system, which utilizes a single objective and has an identical sample interface as an inverted fluorescence microscope with no additional reflection elements. This system has an effective detection NA of > 1.06. We demonstrated multicolor and fast volumetric imaging of live cells and single-molecule super-resolution microscopy using our system.

scholarly journals Liquid Crystal Devices for Beam Steering Applications

Micromachines ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 247
Author(s):  
Rowan Morris ◽  
Cliff Jones ◽  
Mamatha Nagaraj
Keyword(s):  
Liquid Crystal ◽  
Liquid Crystals ◽  
Adaptive Optics ◽  
Beam Steering ◽  
Optical Components ◽  
Advantages And Disadvantages ◽  
Liquid Crystal Devices

Liquid crystals are valuable materials for applications in beam steering devices. In this paper, an overview of the use of liquid crystals in the field of adaptive optics specifically for beam steering and lensing devices is presented. The paper introduces the properties of liquid crystals that have made them useful in this field followed by a more detailed discussion of specific liquid crystal devices that act as switchable optical components of refractive and diffractive types. The relative advantages and disadvantages of the different devices and techniques are summarised.

Hybrid calibration method of a wide-view-angle Mueller polarimeter for hyper-numerical-aperture imaging systems

2020 ◽  
Vol 18 (8) ◽  
pp. 081202
Author(s):  
Jianhui Li ◽  
Yanqiu Li ◽  
Ke Liu ◽  
Guodong Zhou ◽  
Lihui Liu ◽  
...  
Keyword(s):  
Numerical Aperture ◽  
Calibration Method ◽  
Imaging Systems

scholarly journals A miniaturized optical tomography platform for volumetric imaging of engineered living systems

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 550-561 ◽  
Author(s):  
Adem Polat ◽  
Shabir Hassan ◽  
Isa Yildirim ◽  
Luis Eduardo Oliver ◽  
Maryam Mostafaei ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Biological Sample ◽  
Optical Tomography ◽  
Three Dimensional ◽  
Living Systems ◽  
Volumetric Imaging ◽  
Non Invasive ◽  
3D Information

Volumetric optical microscopy approaches that enable acquisition of three-dimensional (3D) information from a biological sample are attractive for numerous non-invasive imaging applications.

scholarly journals Metamaterial Lensing Devices

Molecules ◽  
2019 ◽  
Vol 24 (13) ◽  
pp. 2460 ◽  
Author(s):  
Jiangtao Lv ◽  
Ming Zhou ◽  
Qiongchan Gu ◽  
Xiaoxiao Jiang ◽  
Yu Ying ◽  
...  
Keyword(s):  
Building Blocks ◽  
Biological Imaging ◽  
Transmission Mode ◽  
Two Dimensional ◽  
Optical Components ◽  
Practical Applications ◽  
Perfect Lens ◽  
Significant Potential ◽  
Integrated Optical

In recent years, the development of metamaterials and metasurfaces has drawn great attention, enabling many important practical applications. Focusing and lensing components are of extreme importance because of their significant potential practical applications in biological imaging, display, and nanolithography fabrication. Metafocusing devices using ultrathin structures (also known as metasurfaces) with superlensing performance are key building blocks for developing integrated optical components with ultrasmall dimensions. In this article, we review the metamaterial superlensing devices working in transmission mode from the perfect lens to two-dimensional metasurfaces and present their working principles. Then we summarize important practical applications of metasurfaces, such as plasmonic lithography, holography, and imaging. Different typical designs and their focusing performance are also discussed in detail.

scholarly journals In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei Chen ◽  
Ryan G. Natan ◽  
Yuhan Yang ◽  
Shih-Wei Chou ◽  
Qinrong Zhang ◽  
...  
Keyword(s):  
Adaptive Optics ◽  
Temporal Resolution ◽  
Simultaneous Recording ◽  
High Temporal Resolution ◽  
Spatiotemporal Resolution ◽  
Volumetric Imaging ◽  
High Spatiotemporal Resolution ◽  
Mouse Cortex ◽  
Two Photon Fluorescence

AbstractStudying neuronal activity at synapses requires high spatiotemporal resolution. For high spatial resolution in vivo imaging at depth, adaptive optics (AO) is required to correct sample-induced aberrations. To improve temporal resolution, Bessel focus has been combined with two-photon fluorescence microscopy (2PFM) for fast volumetric imaging at subcellular lateral resolution. To achieve both high-spatial and high-temporal resolution at depth, we develop an efficient AO method that corrects the distorted wavefront of Bessel focus at the objective focal plane and recovers diffraction-limited imaging performance. Applying AO Bessel focus scanning 2PFM to volumetric imaging of zebrafish larval and mouse brains down to 500 µm depth, we demonstrate substantial improvements in the sensitivity and resolution of structural and functional measurements of synapses in vivo. This enables volumetric measurements of synaptic calcium and glutamate activity at high accuracy, including the simultaneous recording of glutamate activity of apical and basal dendritic spines in the mouse cortex.

scholarly journals High-efficiency broadband achromatic metalens for near-IR biological imaging window

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yujie Wang ◽  
Qinmiao Chen ◽  
Wenhong Yang ◽  
Ziheng Ji ◽  
Limin Jin ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Large Scale ◽  
Near Infrared ◽  
High Efficiency ◽  
Numerical Aperture ◽  
Biological Imaging ◽  
Material Loss ◽  
Practical Applications ◽  
Aspect Ratios ◽  
Polarization Insensitive

AbstractOver the past years, broadband achromatic metalenses have been intensively studied due to their great potential for applications in consumer and industry products. Even though significant progress has been made, the efficiency of technologically relevant silicon metalenses is limited by the intrinsic material loss above the bandgap. In turn, the recently proposed achromatic metalens utilizing transparent, high-index materials such as titanium dioxide has been restricted by the small thickness and showed relatively low focusing efficiency at longer wavelengths. Consequently, metalens-based optical imaging in the biological transparency window has so far been severely limited. Herein, we experimentally demonstrate a polarization-insensitive, broadband titanium dioxide achromatic metalens for applications in the near-infrared biological imaging. A large-scale fabrication technology has been developed to produce titanium dioxide nanopillars with record-high aspect ratios featuring pillar heights of 1.5 µm and ~90° vertical sidewalls. The demonstrated metalens exhibits dramatically increased group delay range, and the spectral range of achromatism is substantially extended to the wavelength range of 650–1000 nm with an average efficiency of 77.1%–88.5% and a numerical aperture of 0.24–0.1. This research paves a solid step towards practical applications of flat photonics.

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