scholarly journals A miniaturized optical tomography platform for volumetric imaging of engineered living systems

Lab on a Chip ◽  
2019 ◽  
Vol 19 (4) ◽  
pp. 550-561 ◽  
Author(s):  
Adem Polat ◽  
Shabir Hassan ◽  
Isa Yildirim ◽  
Luis Eduardo Oliver ◽  
Maryam Mostafaei ◽  
...  
Keyword(s):  
Optical Microscopy ◽  
Biological Sample ◽  
Optical Tomography ◽  
Three Dimensional ◽  
Living Systems ◽  
Volumetric Imaging ◽  
Non Invasive ◽  
3D Information

Volumetric optical microscopy approaches that enable acquisition of three-dimensional (3D) information from a biological sample are attractive for numerous non-invasive imaging applications.

scholarly journals Volumetric Lissajous Confocal Microscopy

2019 ◽  
Author(s):  
Takahiro Deguchi ◽  
Paolo Bianchini ◽  
Gemma Palazzolo ◽  
Michele Oneto ◽  
Alberto Diaspro ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Sampling Rate ◽  
Three Dimensional ◽  
Scanning Speed ◽  
Spatiotemporal Resolution ◽  
Volumetric Imaging ◽  
Excitation Beam ◽  
Spatial Changes ◽  
Processing Step ◽  
3D Information

AbstractDynamic biological systems present challenges to existing three-dimensional (3D) optical microscopes because of their continuous temporal and spatial changes. Most techniques are based on rigid architectures, as in confocal microscopy, where a laser beam is sequentially scanned at a predefined spatial sampling rate and pixel dwell time. Here, we developed volumetric Lissajous confocal microscopy to achieve unsurpassed 3D scanning speed with a tunable sampling rate. The system combines an acoustic liquid lens for continuous axial focus translation with a resonant scanning mirror. Accordingly, the excitation beam follows a dynamic Lissajous trajectory enabling sub-millisecond acquisitions of image series containing 3D information at a sub-Nyquist sampling rate. By temporal accumulation and/or advanced interpolation algorithms, volumetric imaging rate is selectable using a post-processing step at the desired spatiotemporal resolution for events of interest. We demonstrate multicolor and calcium imaging over volumes of tens of cubic microns with acquisition speeds up to 5 kHz.

Three-dimensional localisation of DNA in the nucleolus of Spirogyra by correlated optical tomography and serial ultra-thin sectioning

1990 ◽  
Vol 95 (3) ◽  
pp. 343-352
Author(s):  
E.G. Jordan ◽  
D.J. Rawlins
Keyword(s):  
Optical Microscopy ◽  
Optical Tomography ◽  
Three Dimensional ◽  
Dense Fibrillar Component ◽  
Fibrillar Centre ◽  
Thin Sectioning ◽  
Distribution Matching ◽  
Fibrillar Component ◽  
Dapi Fluorescence

Spirogyra nucleoli were shown by three-dimensional optical microscopy of DAPI fluorescence to contain DNA with a pattern and distribution matching those of the fibrillar centres. This was confirmed using different species with nucleoli showing different sizes of fibrillar centre. Much lower levels of fluorescence were seen corresponding to the dense fibrillar component. Nearly all the DAPI fluorescence arises from the fibrillar centres or from regions very close to their surface, indicating that this is the site of nucleolar transcription.

scholarly journals ANALYSIS OF REPEATABILITY ON VIDEOGRAMMETRY FOR INFANTS’ CRANIAL DEFORMATION

2017 ◽  
Author(s):  
Inés Barbero Garcia ◽  
José Luis Lerma ◽  
Ángel Marqués Mateu ◽  
Pablo Miranda
Keyword(s):  
Low Cost ◽  
Measurement Techniques ◽  
Three Dimensional ◽  
Visual Assessment ◽  
Radiological Imaging ◽  
Non Invasive ◽  
Maximum Value ◽  
Magnetic Resonance Imaging Mri ◽  
3D Information ◽  
The Ideal

Cranial deformation affects a large number of infants. The methodologies commonly employed to measure the deformation include, among others, calliper measurements and visual assessment for mild cases and radiological imaging for severe cases, where surgical intervention is considered. Visual assessment and calliper measurements usually lack the required level of accuracy to evaluate the deformation. Radiological imaging, including Computed Tomography (CT) and Magnetic Resonance Imaging (MRI), are costly and highly invasive. The use of smartphones to record videos that can be used for three-dimensional (3D) modelling of the head has emerged as a low-cost, non-invasive methodology to extract 3D information of the patient. To be able to analyse the deformation, a novel technique is employed: the obtained model is compared with an ideal head. In this study we have tested the repeatability of the process. For this purpose, several models of two patients have been obtained and the differences between them are evaluated. The results show that the differences in the ellipsoid semiaxis for the same patient are usually below 4 mm, although they increase up to 6.4 mm in some cases. The variability in the distances to the ideal head, which are the values used to evaluate deformity, reaches a maximum value of 2.7 mm. The errors obtained are comparable to those of classical measurement techniques and show the potential of the methodology in development.http://dx.doi.org/10.4995/CIGeo2017.2017.6604

X-ray microtomography

1988 ◽  
Vol 46 ◽  
pp. 998-999
Author(s):  
H.W. Deckman ◽  
B.F. Flannery ◽  
J.H. Dunsmuir ◽  
K.D' Amico
Keyword(s):  
Computed Tomography ◽  
Internal Structure ◽  
Imaging Technique ◽  
Three Dimensional ◽  
Tissue Structure ◽  
Individual Element ◽  
X Ray ◽  
Non Invasive ◽  
Panoramic Imaging ◽  
Three Dimensional Images

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.

Three-dimensional speckle-tracking echocardiography – a further step in the non-invasive three-dimensional cardiac imaging

2012 ◽  
Vol 153 (40) ◽  
pp. 1570-1577 ◽  
Author(s):  
Attila Nemes ◽  
Anita Kalapos ◽  
Péter Domsik ◽  
Tamás Forster
Keyword(s):  
Cardiac Imaging ◽  
Speckle Tracking ◽  
Speckle Tracking Echocardiography ◽  
Three Dimensional ◽  
Myocardial Mechanics ◽  
Non Invasive ◽  
Imaging Methodology ◽  
Invasive Evaluation

Three-dimensional speckle-tracking echocardiography is a new cardiac imaging methodology, which allows three-dimensional non-invasive evaluation of the myocardial mechanics. The aim of this review is to present this new tool emphasizing its diagnostic potentials and demonstrating its limitations, as well. Orv. Hetil., 2012, 153, 1570–1577.

Electrical Capacitance Volume Tomography (ECVT) for industrial and medical applications-An Overview

2015 ◽  
Vol 11 (1) ◽  
pp. 2897-2908
Author(s):  
Mohammed S.Aljohani
Keyword(s):  
Imaging Technique ◽  
Three Dimensional ◽  
Biological Processes ◽  
Electrical Capacitance ◽  
Image Reconstruction Techniques ◽  
Phase Dynamics ◽  
Non Invasive ◽  
Electrical Capacitance Volume Tomography ◽  
And Performance ◽  
Optimization And Performance

Tomography is a non-invasive, non-intrusive imaging technique allowing the visualization of phase dynamics in industrial and biological processes. This article reviews progress in Electrical Capacitance Volume Tomography (ECVT). ECVT is a direct 3D visualizing technique, unlike three-dimensional imaging, which is based on stacking 2D images to obtain an interpolated 3D image. ECVT has recently matured for real time, non-invasive 3-D monitoring of processes involving materials with strong contrast in dielectric permittivity. In this article, ECVT sensor design, optimization and performance of various sensors seen in literature are summarized. Qualitative Analysis of ECVT image reconstruction techniques has also been presented.

Analyzing the Impact of X-Ray Tomography on the Reliability of Integrated Circuits

2015 ◽  
Author(s):  
Halit Dogan ◽  
Md Mahbub Alam ◽  
Navid Asadizanjani ◽  
Sina Shahbazmohamadi ◽  
Domenic Forte ◽  
...  
Keyword(s):  
Integrated Circuits ◽  
Integrated Circuit ◽  
3D Imaging ◽  
Three Dimensional ◽  
Serial Sectioning ◽  
Promising Technique ◽  
Flash Memories ◽  
X Ray ◽  
3D Information ◽  
The Impact

Abstract X-ray tomography is a promising technique that can provide micron level, internal structure, and three dimensional (3D) information of an integrated circuit (IC) component without the need for serial sectioning or decapsulation. This is especially useful for counterfeit IC detection as demonstrated by recent work. Although the components remain physically intact during tomography, the effect of radiation on the electrical functionality is not yet fully investigated. In this paper we analyze the impact of X-ray tomography on the reliability of ICs with different fabrication technologies. We perform a 3D imaging using an advanced X-ray machine on Intel flash memories, Macronix flash memories, Xilinx Spartan 3 and Spartan 6 FPGAs. Electrical functionalities are then tested in a systematic procedure after each round of tomography to estimate the impact of X-ray on Flash erase time, read margin, and program operation, and the frequencies of ring oscillators in the FPGAs. A major finding is that erase times for flash memories of older technology are significantly degraded when exposed to tomography, eventually resulting in failure. However, the flash and Xilinx FPGAs of newer technologies seem less sensitive to tomography, as only minor degradations are observed. Further, we did not identify permanent failures for any chips in the time needed to perform tomography for counterfeit detection (approximately 2 hours).

scholarly journals Non-invasive three-dimensional thickness analysis of oral epithelium based on optical coherence tomography—development and diagnostic performance

Heliyon ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. e06645
Author(s):  
Charlotte Theresa Trebing ◽  
Sinan Sen ◽  
Stefan Rues ◽  
Christopher Herpel ◽  
Maria Schöllhorn ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Diagnostic Performance ◽  
Three Dimensional ◽  
Oral Epithelium ◽  
Optical Coherence ◽  
Non Invasive

scholarly journals Elastic transformation of histological slices allows precise co-registration with microCT data sets for a refined virtual histology approach

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jonas Albers ◽  
Angelika Svetlove ◽  
Justus Alves ◽  
Alexander Kraupner ◽  
Francesca di Lillo ◽  
...  
Keyword(s):  
Three Dimensional ◽  
High Specificity ◽  
Histopathological Analysis ◽  
Specific Information ◽  
Data Sets ◽  
Virtual Histology ◽  
Novel Approach ◽  
Cell Tissue ◽  
Immuno Histochemistry ◽  
3D Information

AbstractAlthough X-ray based 3D virtual histology is an emerging tool for the analysis of biological tissue, it falls short in terms of specificity when compared to conventional histology. Thus, the aim was to establish a novel approach that combines 3D information provided by microCT with high specificity that only (immuno-)histochemistry can offer. For this purpose, we developed a software frontend, which utilises an elastic transformation technique to accurately co-register various histological and immunohistochemical stainings with free propagation phase contrast synchrotron radiation microCT. We demonstrate that the precision of the overlay of both imaging modalities is significantly improved by performing our elastic registration workflow, as evidenced by calculation of the displacement index. To illustrate the need for an elastic co-registration approach we examined specimens from a mouse model of breast cancer with injected metal-based nanoparticles. Using the elastic transformation pipeline, we were able to co-localise the nanoparticles to specifically stained cells or tissue structures into their three-dimensional anatomical context. Additionally, we performed a semi-automated tissue structure and cell classification. This workflow provides new insights on histopathological analysis by combining CT specific three-dimensional information with cell/tissue specific information provided by classical histology.

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