scholarly journals Identification of a novel putative interaction partner of the nucleoporin ALADIN

2016 ◽  
Author(s):  
Ramona Jühlen ◽  
Dana Landgraf ◽  
Angela Huebner ◽  
Katrin Koehler
Keyword(s):  
Cell Cycle ◽  
Redox Homeostasis ◽  
Proteome Analysis ◽  
Spindle Assembly ◽  
Microsomal Protein ◽  
Interaction Partner ◽  
Meiotic Spindle ◽  
Future Research ◽  
Tumour Cell Line ◽  
Triple A Syndrome

AbstractIt has been shown that the nucleoporin ALADIN employs a significant role in the redox homeostasis of the cell but the function in steroidogenesis contributing to adrenal atrophy in triple A syndrome remains largely unknown. In an attempt to identify new interaction partners of ALADIN, co-immunoprecipitation followed by proteome analysis was conducted in different expression models using the human adrenocortical tumour cell line NCI-H295R. Our results suggest an interaction of ALADIN with the microsomal protein PGRMC2. PGRMC2 is shown to be activity regulator of CYP P450 enzymes and therefore, to be a possible target for adrenal dysregulation in triple A syndrome. We show that there is a sexual dimorphism regarding the expression of Pgrmc2 in adrenals and gonads of WT and Aaas KO mice. Female Aaas KO mice are sterile due to delayed oocyte maturation and meiotic spindle assembly. A participation in meiotic spindle assembly confirms the recently investigated involvement of ALADIN in mitosis and emphasises an interaction with PGRMC2 which is a regulator of cell cycle. By identification of a novel interaction partner of ALADIN we provide novel aspects for future research of the function of ALADIN during cell cycle and for new insights into the pathogenesis of triple A syndrome.

Bora regulates meiotic spindle assembly and cell cycle during mouse oocyte meiosis

2013 ◽  
Vol 80 (6) ◽  
pp. 474-487 ◽  
Author(s):  
Rui Zhai ◽  
Yi-Feng Yuan ◽  
Yi Zhao ◽  
Xiao-Ming Liu ◽  
Yan-Hong Zhen ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Spindle Assembly ◽  
Mouse Oocyte ◽  
Meiotic Spindle ◽  
Oocyte Meiosis

scholarly journals Inherited Defects of the ASC-1 Complex in Congenital Neuromuscular Diseases

2021 ◽  
Vol 22 (11) ◽  
pp. 6039
Author(s):  
Justine Meunier ◽  
Rocio-Nur Villar-Quiles ◽  
Isabelle Duband-Goulet ◽  
Ana Ferreiro
Keyword(s):  
Cell Cycle ◽  
Striated Muscle ◽  
Bone Fractures ◽  
Muscular Atrophy ◽  
Neuromuscular Diseases ◽  
Muscle Disease ◽  
Future Research ◽  
Muscle Involvement ◽  
Functional Studies ◽  
Monogenic Disorders

Defects in transcriptional and cell cycle regulation have emerged as novel pathophysiological mechanisms in congenital neuromuscular disease with the recent identification of mutations in the TRIP4 and ASCC1 genes, encoding, respectively, ASC-1 and ASCC1, two subunits of the ASC-1 (Activating Signal Cointegrator-1) complex. This complex is a poorly known transcriptional coregulator involved in transcriptional, post-transcriptional or translational activities. Inherited defects in components of the ASC-1 complex have been associated with several autosomal recessive phenotypes, including severe and mild forms of striated muscle disease (congenital myopathy with or without myocardial involvement), but also cases diagnosed of motor neuron disease (spinal muscular atrophy). Additionally, antenatal bone fractures were present in the reported patients with ASCC1 mutations. Functional studies revealed that the ASC-1 subunit is a novel regulator of cell cycle, proliferation and growth in muscle and non-muscular cells. In this review, we summarize and discuss the available data on the clinical and histopathological phenotypes associated with inherited defects of the ASC-1 complex proteins, the known genotype–phenotype correlations, the ASC-1 pathophysiological role, the puzzling question of motoneuron versus primary muscle involvement and potential future research avenues, illustrating the study of rare monogenic disorders as an interesting model paradigm to understand major physiological processes.

The tumor suppressor LZTS2 functions through the cellular samurai Katanin

2009 ◽  
Vol 4 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Yoshiro Maru
Keyword(s):  
Cell Cycle ◽  
Tumor Suppressor ◽  
Leucine Zipper ◽  
Biological Effects ◽  
Regulatory Subunit ◽  
Future Research ◽  
Central Spindle ◽  
Microtubule Severing ◽  
Putative Tumor Suppressor ◽  
Potential Area

AbstractThe leucine zipper putative tumor suppressor (LZTS) 2 is frequently and specifically found in LOH (loss of heterozygosity) analysis in cancer. Different from other LZTS family members, it regulates the microtubule-severing protein Katanin by binding the p80 regulatory subunit of Katanin and inhibiting its interaction with microtubules. At specific phases of the cell cycle, LZTS2 suppresses cell migration and establishes proper central spindle assembly for cytokinesis. Importantly, those biological effects are mediated by the inhibitory activity of LZTS2 on Katanin. LZTS2 binding to Katanin also plays a role in Katanin transport to the midbody to control proper abscission. Therapeutic applications of the interaction between LZTS2 and Katanin in tumor cells are a potential area for future research.

scholarly journals Effects of Independent and Combined Water-Deficit and High-Nitrogen Treatments on Flag Leaf Proteomes during Wheat Grain Development

2020 ◽  
Vol 21 (6) ◽  
pp. 2098 ◽  
Author(s):  
Dong Zhu ◽  
Gengrui Zhu ◽  
Zhen Zhang ◽  
Zhimin Wang ◽  
Xing Yan ◽  
...  
Keyword(s):  
Carbohydrate Metabolism ◽  
Water Deficit ◽  
Flag Leaf ◽  
Redox Homeostasis ◽  
Combined Treatment ◽  
Proteome Analysis ◽  
N Fertilization ◽  
Grain Development ◽  
High Nitrogen ◽  
Nitrogen Treatments

We present the first comprehensive proteome analysis of wheat flag leaves under water-deficit, high-nitrogen (N) fertilization, and combined treatments during grain development in the field. Physiological and agronomic trait analyses showed that leaf relative water content, total chlorophyll content, photosynthetic efficiency, and grain weight and yield were significantly reduced under water-deficit conditions, but dramatically enhanced under high-N fertilization and moderately promoted under the combined treatment. Two-dimensional electrophoresis detected 72 differentially accumulated protein (DAP) spots representing 65 unique proteins, primarily involved in photosynthesis, signal transduction, carbohydrate metabolism, redox homeostasis, stress defense, and energy metabolism. DAPs associated with photosynthesis and protein folding showed significant downregulation and upregulation in response to water-deficit and high-N treatments, respectively. The combined treatment caused a moderate upregulation of DAPs related to photosynthesis and energy and carbohydrate metabolism, suggesting that high-N fertilization can alleviate losses in yield caused by water-deficit conditions by enhancing leaf photosynthesis and grain storage compound synthesis.

Cell cycle regulation of central spindle assembly

Nature ◽  
2004 ◽  
Vol 430 (7002) ◽  
pp. 908-913 ◽  
Author(s):  
Masanori Mishima ◽  
Visnja Pavicic ◽  
Ulrike Grüneberg ◽  
Erich A. Nigg ◽  
Michael Glotzer
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Regulation ◽  
Spindle Assembly ◽  
Central Spindle ◽  
Cycle Regulation
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