scholarly journals The P25 protein of Potato virus X suppresses accumulation of 24nt siRNAs in the N. benthamiana transgenic line 16c

2015 ◽  
Author(s):  
Shahinez Garcia ◽  
Christophe Himber ◽  
Olivier Voinnet
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Rna Silencing ◽  
Transgenic Line ◽  
Potato Virus X ◽  
Positive Correlation ◽  
Viral Suppressors ◽  
Background Pixel ◽  
Systemic Onset ◽  
Local Accumulation

By transiently expressing viral suppressors of RNA silencing (VSRs) in combination with GFP silencing-inducing constructs in leaves of Nicotiana benthamiana line 16c, Hamilton et al. (2002) could establish a positive correlation between the production of 24nt GFP small interfering (si)RNAs in infiltrated leaves and the systemic onset of GFP silencing in remote tissues. In the context of GFP silencing inducers based on replicating Potato virus X (PVX), the P25 protein of PVX was found to specifically inhibit the local accumulation of 24nt GFP siRNAs. In the original paper, there were background pixel pattern duplications in the figure reporting the P25 experiments. We have now repeated these experiments with the original clones and the results presented here confirm those reported in the original paper.

scholarly journals Phenotypes and Functional Effects Caused by Various Viral RNA Silencing Suppressors in Transgenic Nicotiana benthamiana and N. tabacum

2008 ◽  
Vol 21 (2) ◽  
pp. 178-187 ◽  
Author(s):  
Shahid Aslam Siddiqui ◽  
Cecilia Sarmiento ◽  
Erkki Truve ◽  
Harry Lehto ◽  
Kirsi Lehto
Keyword(s):  
Mosaic Virus ◽  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Rna Silencing ◽  
Fluorescent Protein ◽  
Potato Virus X ◽  
Mottle Virus ◽  
African Cassava Mosaic Virus ◽  
Rice Yellow Mottle Virus ◽  
Green Fluorescent

RNA silencing suppressor genes derived from six virus genera were transformed into Nicotiana benthamiana and N. tabacum plants. These suppressors were P1 of Rice yellow mottle virus (RYMV), P1 of Cocksfoot mottle virus, P19 of Tomato bushy stunt virus, P25 of Potato virus X, HcPro of Potato virus Y (strain N), 2b of Cucumber mosaic virus (strain Kin), and AC2 of African cassava mosaic virus (ACMV). HcPro caused the most severe phenotypes in both Nicotiana spp. AC2 also produced severe effects in N. tabacum but a much milder phenotype in N. benthamiana, although both HcPro and AC2 affected the leaf tissues of the two Nicotiana spp. in similar ways, causing hyperplasia and hypoplasia, respectively. P1-RYMV caused high lethality in the N. benthamiana plants but only mild effects in the N. tabacum plants. Phenotypic alterations produced by the other transgenes were minor in both species. Interestingly, the suppressors had very different effects on crucifer-infecting Tobamovirus (crTMV) infections. AC2 enhanced both spread and brightness of the crTMV-green fluorescent protein (GFP) lesions, whereas 2b and both P1 suppressors enhanced spread but not brightness of these lesions. P19 promoted spread of the infection into new foci within the infiltrated leaf, whereas HcPro and P25 suppressed the spread of crTMV-GFP lesions.

scholarly journals Inhibition of RNA silencing by the coat protein of Pelargonium flower break virus: distinctions from closely related suppressors

2009 ◽  
Vol 90 (2) ◽  
pp. 519-525 ◽  
Author(s):  
Sandra Martínez-Turiño ◽  
Carmen Hernández
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Rna Silencing ◽  
Potato Virus X ◽  
Plant Viruses ◽  
Small Interfering Rnas ◽  
Silencing Suppression ◽  
Viral Suppressors

Viral-derived double-stranded RNAs (dsRNAs) activate RNA silencing, generating small interfering RNAs (siRNAs) which are incorporated into an RNA-induced silencing complex (RISC) that promotes homology-dependent degradation of cognate RNAs. To counteract this, plant viruses express RNA silencing suppressors. Here, we show that the coat protein (CP) of Pelargonium flower break virus (PFBV), a member of the genus Carmovirus, is able to efficiently inhibit RNA silencing. Interestingly, PFBV CP blocked both sense RNA- and dsRNA-triggered RNA silencing and did not preclude generation of siRNAs, which is in contrast with the abilities that have been reported for other carmoviral CPs. We have also found that PFBV CP can bind siRNAs and that this ability correlates with silencing suppression activity and enhancement of potato virus X pathogenicity. Collectively, the results indicate that PFBV CP inhibits RNA silencing by sequestering siRNAs and preventing their incorporation into a RISC, thus behaving similarly to unrelated viral suppressors but dissimilarly to orthologous ones.

scholarly journals Rice Stripe Mosaic Virus–Encoded P4 Is a Weak Suppressor of Viral RNA Silencing and Is Required for Disease Symptom Development

2020 ◽  
Vol 33 (3) ◽  
pp. 412-422
Author(s):  
Chao Zhang ◽  
Dong Chen ◽  
Guoyi Yang ◽  
Xiyuan Yu ◽  
Jianguo Wu
Keyword(s):  
Mosaic Virus ◽  
Rna Silencing ◽  
Matrix Protein ◽  
Expression System ◽  
Potato Virus X ◽  
Bimolecular Fluorescence Complementation ◽  
Developmental Defects ◽  
The Matrix ◽  
Silencing Pathways ◽  
Viral Suppressors

Viral suppressors of RNA silencing (VSRs) are a cluster of viral proteins that have evolved to counteract eukaryotic antiviral RNA silencing pathways, thereby contributing to viral pathogenicity. In this study, we revealed that the matrix protein P4 encoded by rice stripe mosaic virus (RSMV), which is an emerging cytoplasmic rhabdovirus, is a weak RNA silencing suppressor. By conducting yeast two-hybrid, bimolecular fluorescence complementation, and subcellular colocalization assays, we proved that P4 interacts with the rice endogenous suppressor of gene silencing 3 (OsSGS3). We also determined that P4 overexpression has no effect on OsSGS3 transcription. However, P4 can promote the degradation of OsSGS3 via ubiquitination and autophagy. Additionally, a potato virus X–based expression system was used to confirm that P4 enhances the development of mosaic symptoms on Nicotiana benthamiana leaves by promoting hydrogen peroxide accumulation but not cell death. To verify whether P4 is a pathogenicity factor in host plants, we generated transgenic P4-overexpressing rice plants that exhibited disease-related developmental defects including decreased plant height and excessive tillering. Our data suggest that RSMV-encoded P4 serves as a weak VSR that inhibits antiviral RNA silencing by targeting OsSGS3.

scholarly journals Interplay between potato virus X and RNA granules in Nicotiana benthamiana

2020 ◽  
Vol 276 ◽  
pp. 197823
Author(s):  
Gabriel Robles-Luna ◽  
Nicolás Furman ◽  
María Florencia Barbarich ◽  
Nicolás Carlotto ◽  
Alejandra Attorresi ◽  
...  
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Rna Granules

scholarly journals Soluble, Template-Dependent Extracts from Nicotiana benthamiana Plants Infected with Potato Virus X Transcribe both Plus- and Minus-Strand RNA Templates

Virology ◽  
2000 ◽  
Vol 275 (2) ◽  
pp. 444-451 ◽  
Author(s):  
Carol A. Plante ◽  
Kook-Hyung Kim ◽  
Neeta Pillai-Nair ◽  
Toba A.M. Osman ◽  
Kenneth W. Buck ◽  
...  
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Minus Strand

Efficient transient expression of human GM-CSF protein in Nicotiana benthamiana using potato virus X vector

2006 ◽  
Vol 72 (4) ◽  
pp. 756-762 ◽  
Author(s):  
Fengyong Zhou ◽  
Ming-Li Wang ◽  
Henrik H. Albert ◽  
Paul H. Moore ◽  
Yun J. Zhu
Keyword(s):  
Transient Expression ◽  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Gm Csf

scholarly journals A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ran Li ◽  
Chuan-Sia Tee ◽  
Yu-Lin Jiang ◽  
Xi-Yuan Jiang ◽  
Prasanna Nori Venkatesh ◽  
...  
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Basal Defense
Sign in / Sign up

Export Citation Format

Share Document