scholarly journals A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Ran Li ◽  
Chuan-Sia Tee ◽  
Yu-Lin Jiang ◽  
Xi-Yuan Jiang ◽  
Prasanna Nori Venkatesh ◽  
...  
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Basal Defense

scholarly journals Interplay between potato virus X and RNA granules in Nicotiana benthamiana

2020 ◽  
Vol 276 ◽  
pp. 197823
Author(s):  
Gabriel Robles-Luna ◽  
Nicolás Furman ◽  
María Florencia Barbarich ◽  
Nicolás Carlotto ◽  
Alejandra Attorresi ◽  
...  
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Rna Granules

scholarly journals Phenotypes and Functional Effects Caused by Various Viral RNA Silencing Suppressors in Transgenic Nicotiana benthamiana and N. tabacum

2008 ◽  
Vol 21 (2) ◽  
pp. 178-187 ◽  
Author(s):  
Shahid Aslam Siddiqui ◽  
Cecilia Sarmiento ◽  
Erkki Truve ◽  
Harry Lehto ◽  
Kirsi Lehto
Keyword(s):  
Mosaic Virus ◽  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Rna Silencing ◽  
Fluorescent Protein ◽  
Potato Virus X ◽  
Mottle Virus ◽  
African Cassava Mosaic Virus ◽  
Rice Yellow Mottle Virus ◽  
Green Fluorescent

RNA silencing suppressor genes derived from six virus genera were transformed into Nicotiana benthamiana and N. tabacum plants. These suppressors were P1 of Rice yellow mottle virus (RYMV), P1 of Cocksfoot mottle virus, P19 of Tomato bushy stunt virus, P25 of Potato virus X, HcPro of Potato virus Y (strain N), 2b of Cucumber mosaic virus (strain Kin), and AC2 of African cassava mosaic virus (ACMV). HcPro caused the most severe phenotypes in both Nicotiana spp. AC2 also produced severe effects in N. tabacum but a much milder phenotype in N. benthamiana, although both HcPro and AC2 affected the leaf tissues of the two Nicotiana spp. in similar ways, causing hyperplasia and hypoplasia, respectively. P1-RYMV caused high lethality in the N. benthamiana plants but only mild effects in the N. tabacum plants. Phenotypic alterations produced by the other transgenes were minor in both species. Interestingly, the suppressors had very different effects on crucifer-infecting Tobamovirus (crTMV) infections. AC2 enhanced both spread and brightness of the crTMV-green fluorescent protein (GFP) lesions, whereas 2b and both P1 suppressors enhanced spread but not brightness of these lesions. P19 promoted spread of the infection into new foci within the infiltrated leaf, whereas HcPro and P25 suppressed the spread of crTMV-GFP lesions.

scholarly journals Soluble, Template-Dependent Extracts from Nicotiana benthamiana Plants Infected with Potato Virus X Transcribe both Plus- and Minus-Strand RNA Templates

Virology ◽  
2000 ◽  
Vol 275 (2) ◽  
pp. 444-451 ◽  
Author(s):  
Carol A. Plante ◽  
Kook-Hyung Kim ◽  
Neeta Pillai-Nair ◽  
Toba A.M. Osman ◽  
Kenneth W. Buck ◽  
...  
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Minus Strand

Efficient transient expression of human GM-CSF protein in Nicotiana benthamiana using potato virus X vector

2006 ◽  
Vol 72 (4) ◽  
pp. 756-762 ◽  
Author(s):  
Fengyong Zhou ◽  
Ming-Li Wang ◽  
Henrik H. Albert ◽  
Paul H. Moore ◽  
Yun J. Zhu
Keyword(s):  
Transient Expression ◽  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Gm Csf

scholarly journals Production of Antimicrobial Defensin in Nicotiana benthamiana with a Potato Virus X Vector

2001 ◽  
Vol 14 (2) ◽  
pp. 111-115 ◽  
Author(s):  
Hiromasa Saitoh ◽  
Akinori Kiba ◽  
Masahiro Nishihara ◽  
Saburo Yamamura ◽  
Kazumi Suzuki ◽  
...  
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Magnaporthe Grisea ◽  
Expression System ◽  
Potato Virus X ◽  
Phytopathogenic Fungi ◽  
Gene Encoding ◽  
Phytopathogenic Bacterium ◽  
Pseudomonas Cichorii ◽  
Wasabia Japonica

A recombinant plasmid, pTXS.TH, was constructed to express the gene-encoding wasabi (Wasabia japonica) defensin with the potato virus X (PVX) vector. pTXS.TH allows the expression of defensin in the host Nicotiana benthamiana, and the defensin protein WT1 can be purified from virus-infected leaves by heat treatment and affinity chromatography. WT1 exhibits strong antifungal activity toward the phytopathogenic fungi Magnaporthe grisea(50% inhibitory concentration [IC50] = 5 μg/ml) and Botrytis cinerea (IC50 = 20 μg/ml) but is weakly active against the phytopathogenic bacterium Pseudomonas cichorii. This virus-mediated expression system is a rapid and efficient method to produce and characterize antimicrobial proteins in plants. It is particularly useful for the study of proteins that are difficult to produce with other expression systems.

scholarly journals SGT1 Is Induced by the Potato virus X TGBp3 and Enhances Virus Accumulation in Nicotiana benthamiana

2012 ◽  
Vol 5 (5) ◽  
pp. 1151-1153 ◽  
Author(s):  
Chang-Ming Ye ◽  
Vicky Kelly ◽  
Mark Payton ◽  
Martin B. Dickman ◽  
Jeanmarie Verchot
Keyword(s):  
Potato Virus ◽  
Nicotiana Benthamiana ◽  
Potato Virus X ◽  
Virus Accumulation
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