scholarly journals Application of HPLC Combined with Laser Induced Fluorescence for Protein Profile Analysis of Tissue Homogenates in Cervical Cancer

2012 ◽  
Vol 2012 ◽  
pp. 1-7
Author(s):  
Sujatha Bhat ◽  
Ajeetkumar Patil ◽  
Lavanya Rai ◽  
V. B. Kartha ◽  
Santhosh Chidangil
Keyword(s):  
Cervical Cancer ◽  
Diagnostic Accuracy ◽  
High Performance ◽  
Profile Analysis ◽  
Protein Profile ◽  
Laser Induced Fluorescence ◽  
Cancer Tissue ◽  
Protein Profiles ◽  
Tissue Homogenates ◽  
Youden’S Index

A highly objective method, High Performance Liquid Chromatography with Laser Induced Fluorescence (HPLC-LIF) technique was used to study the protein profiles of normal and cervical cancer tissue homogenates. A total of 44 samples including normal cervical biopsy samples from the hysterectomy patients and the patients suffering from different stages of the cervical cancer were recorded by HPLC-LIF and analysed by Principle Component Analysis (PCA) to get statistical information on different tissue components. Discrimination of different stages of the samples was carried out by considering three parameters—scores of factor, spectral residual, and Mahalanobis Distance. Diagnostic accuracy of the method was evaluated using Receiver Operating Characteristic (ROC) analysis, and Youden's index(J)plots. The PCA results showed high sensitivity and specificity (∼100) for cervical cancer diagnosis. ROC and Youden's index curves for both normal and malignant standard sets show good diagnostic accuracy with high AUC values. The statistical analysis has shown that the differences in protein profiles can be used to diagnose biochemical changes in the tissue, and thus can be readily applied for the detection of cervical cancer, even in situations where a histopathology examination is not easy because of nonavailability of experienced pathologists.

Objective Diagnosis of Cervical Cancer by Tissue Protein Profile Analysis

2011 ◽  
Author(s):  
Ajeetkumar Patil ◽  
Sujatha Bhat ◽  
Lavanya Rai ◽  
V. B. Kartha ◽  
Santhosh Chidangil ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Profile Analysis ◽  
Protein Profile ◽  
Tissue Protein

Protein profile analysis of cellular samples from the cervix for the objective diagnosis of cervical cancer using HPLC-LIF

2010 ◽  
Vol 878 (31) ◽  
pp. 3225-3230 ◽  
Author(s):  
Sujatha Bhat ◽  
Ajeetkumar Patil ◽  
Lavanya Rai ◽  
V.B. Kartha ◽  
C. Santhosh
Keyword(s):  
Cervical Cancer ◽  
Profile Analysis ◽  
Protein Profile

scholarly journals Effect Acupuncture for Low Back Pain; Biochemical and Protein Profile Analysis

2020 ◽  
Vol 16 (2) ◽  
pp. 207-215
Author(s):  
Suwaji Handaru Wardoyo ◽  
Solichan Badri
Keyword(s):  
Low Back Pain ◽  
Blood Serum ◽  
Total Protein ◽  
Profile Analysis ◽  
Protein Profile ◽  
Protein Band ◽  
Low Back ◽  
Protein Profiles ◽  
Sds Page ◽  
Blood Serum Protein

Low back pain (LBP) is one of the common symptoms experienced by some of the world community. Acupuncture as a method of healing a disease offers a solution for LBP symptom. This study aimed to determine the effect of acupuncture with local and Yamamoto New Scalp Acupuncture (YNSA) points with biochemical analysis related to total protein, albumin, and globulin, and analysis of blood serum protein profiles using the SDS-PAGE method. This research was conducted using sterile acupuncture needles that inserted in patients with several local points, namely BL22 (Sanjiaoshu), BL23 (Shenshu), BL24 (Qihaishu), BL25C (Dachangshu), and BL26C (Guanyuanshu) and combined with the YNSA points through somatotope D points and extra lumbar points H and I. Blood serum was collected for total protein, albumin, and globulin analysis. That blood serum was also used for protein profile analysis using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) method. The results showed that six times of acupuncture treatments gave positive correlation in total protein, albumin, and globulin. Analysis of blood serum protein profiles using SDS-PAGE showed a protein band of about 12 KDa and it might be a interleukin-13 protein. The results were expected can be useful as scientific information especially related to acupuncture for LBP treatment and this of about 12 KDa protein band can be used as biomarker candidates for the symptoms.

scholarly journals Integrative Systems Biology Approaches to Identify Potential Biomarkers and Pathways of Cervical Cancer

2021 ◽  
Vol 11 (5) ◽  
pp. 363
Author(s):  
Arafat Rahman Oany ◽  
Mamun Mia ◽  
Tahmina Pervin ◽  
Salem Ali Alyami ◽  
Mohammad Ali Moni
Keyword(s):  
Cervical Cancer ◽  
Systems Biology ◽  
Differentially Expressed Genes ◽  
Inflammatory Responses ◽  
Profile Analysis ◽  
Target Identification ◽  
Regulatory Genes ◽  
Differentially Expressed ◽  
Hub Genes ◽  
Potential Biomarkers

Nowadays, cervical cancer (CC) is treated as the leading cancer among women throughout the world. Despite effective vaccination and improved surgery and treatment, CC retains its fatality rate of about half of the infected population globally. The major screening biomarkers and therapeutic target identification have now become a global concern. In the present study, we have employed systems biology approaches to retrieve the potential biomarkers and pathways from transcriptomic profiling. Initially, we have identified 76 of each up-regulated and down-regulated gene from a total of 4643 differentially expressed genes. The up-regulatory genes mainly concentrate on immune-inflammatory responses, and the down-regulatory genes are on receptor binding and gamma-glutamyltransferase. The involved pathways associated with these genes were also assessed through pathway enrichment, and we mainly focused on different cancer pathways, immunoresponse, and cell cycle pathways. After the subsequent enrichment of these genes, we have identified 12 hub genes, which play a crucial role in CC and are verified by expression profile analysis. From our study, we have found that genes LILRB2 and CYBB play crucial roles in CC, as reported here for the first time. Furthermore, the survivability of the hub genes was also assessed, and among them, finally, CXCR4 has been identified as one of the most potential differentially expressed genes that might play a vital role in the survival of CC patients. Thus, CXCR4 could be used as a prognostic and/or diagnostic biomarker and a drug target for CC.

scholarly journals Root iTRAQ protein profile analysis of two Citrus species differing in aluminum-tolerance in response to long-term aluminum-toxicity

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Huan-Xin Jiang ◽  
Lin-Tong Yang ◽  
Yi-Ping Qi ◽  
Yi-Bin Lu ◽  
Zeng-Rong Huang ◽  
...  
Keyword(s):  
Aluminum Toxicity ◽  
Profile Analysis ◽  
Protein Profile ◽  
Aluminum Tolerance ◽  
Citrus Species
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