Protein profile study of Pap smear and tissue of cervix by high performance liquid chromatography: laser induced fluorescence

2007 ◽  
Author(s):  
Sujatha ◽  
Lavanya Rai ◽  
Pratap Kumar ◽  
B. R. Krishnanand ◽  
K. K. Mahato ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Pap Smear ◽  
Protein Profile ◽  
Laser Induced Fluorescence ◽  
Profile Study

scholarly journals Highly Sensitive High Performance Liquid Chromatography-Laser Induced Fluorescence for Proteomics Applications

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Ajeetkumar Patil ◽  
K. S. Choudhari ◽  
Vijendra Prabhu ◽  
V. K. Unnikrishnan ◽  
Sujatha Bhat ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Serum Proteins ◽  
Sensitivity Study ◽  
Laser Induced Fluorescence ◽  
Laser Induced Fluorescence Detection ◽  
Highly Sensitive ◽  
Sds Page ◽  
And Performance

This paper describes the sensitivity study and performance evaluation of high-performance liquid chromatography-laser-induced fluorescence detection (HPLC-LIF) system assembled in our laboratory for proteomics applications. The limits of Detection (LOD) of several serum proteins have been estimated with this instrument and are found to be much lower compared to other commonly used proteomics techniques like SELDI, MALDI, 2-D-SDS-PAGE, and so forth. Techniques for improving the LOD still further with similar setup are briefly discussed. Using the system, protein profiles of serum in normal, malignant, and premalignant conditions were recorded for different malignancy situations.

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