scholarly journals The Effects of Subchronic Exposure to Terbuthylazine on Early Developmental Stages of Common Carp

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Stanislava Štěpánová ◽  
Lucie Plhalová ◽  
Petra Doleželová ◽  
Miroslav Prokeš ◽  
Petr Maršálek ◽  
...  

The aim of this study was to assess the impact of terbuthylazine in surface waters on fish under experimental conditions. Subchronic toxic effects on embryos and larvae of common carp (Cyprinus carpio) were investigated during a 30-day toxicity test. The exposure to terbuthylazin showed no effect on mortality, but significant differences (P<0.0001) were revealed on weight and growth parameters at concentrations of 520 and 820 μg/L. The inhibition of specific growth rate at concentrations of 520 and 820 μg/L was 14% compared to the control group. No significant negative effects on total body length and body weight were observed at lower concentrations (0.9 and 160 μg/L). The concentrations 520 and 820 μg/L were associated with a delay in development compared to other experimental groups and controls. On the basis of weight and growth rate evaluation and determination of developmental stages, the No Observed Effect Concentration (NOEC) of terbuthylazine was estimated at 160 μg/L and the Lowest Observed Effect Concentration (LOEC) was 520 μg/L. According to these results, the reported environmental concentration of terbuthylazine in Czech rivers does not impact growth, development, morphology, or histology of carp embryos and larvae.

2013 ◽  
Vol 82 (3) ◽  
pp. 343-347 ◽  
Author(s):  
Eva Prášková ◽  
Stanislava Štěpánová ◽  
Lucie Chromcová ◽  
Lucie Plhalová ◽  
Eva Voslářová ◽  
...  

Ketoprofen residues have been found in surface water where they present a potential risk to nontarget aquatic species. The aim of this study was to assess the impact of ketoprofen in surface waters on fish under experimental conditions. Subchronic toxic effects on 300 embryos and larvae of common carp (Cyprinus carpio) were investigated during a 30 day toxicity test with concentrations of ketoprofen: 0.003, 2.1, 6.3 and 21 mg/l. The exposure to ketoprofen showed no effect on mortality, but we observed significant delay (P < 0.05) in hatching in day 3 in fish exposed to all concentrations of ketoprofen. Significant delays (P < 0.05) in development were revealed at 2.1, 6.3 and 21 mg/l ketoprofen. On the basis of weight and growth rate evaluation and the determination of developmental stages, the lowest observed effect concentration was 0.003 mg/l. According to these results, the reported environmental concentration of ketoprofen in Czech rivers could have a negative effect on the growth and development of carp embryos and larvae. These tests have not yet been performed. Further research and search for a mechanism to reduce the incidence of ketoprofen in the waters by better wastewater treatment is required.


2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Josef Velisek ◽  
Alzbeta Stara ◽  
Dalibor Koutnik ◽  
Jana Machova

The aim of the study was to investigate effects of the triazine’s herbicide terbuthylazine-2-hydroxy on early life stage of common carp (Cyprinus carpioL.) through antioxidant indices, mortality, growth, development, and histopathology. Based on accumulated mortality in the experimental groups, lethal concentrations of terbuthylazine-2-hydroxy were estimated at 35-day LC50 = 10.9 mg/L terbuthylazine-2-hydroxy. By day 15, fish were exposed to 3.5 mg/L and by day 26, fish were exposed to 0.0029 mg/L; real environmental concentration in Czech rivers, 0.07 mg/L, 1.4 mg/L, and 3.5 mg/L terbuthylazine-2-hydroxy, showed significantly lower mass and total length compared with controls. Based on inhibition of growth in the experimental groups, lowest observed effect concentration (LOEC) = 0.002 mg/L terbuthylazine-2-hydroxy and no observed effect concentration (NOEC) = 0.0001 mg/L terbuthylazine-2-hydroxy. No significant negative effects on hatching or embryo viability were demonstrated at the concentrations tested, but significant differences in early ontogeny among groups were noted. Fish from the two highest tested concentrations showed a dose-related delay in development compared with the controls. Total superoxide dismutase (SOD) activity was significant lower in all groups testedly for terbuthylazine-2-hydroxy compared with the control group. At concentrations of 1.4 and 3.5 mg/L damage to caudal kidney tubules when compared to control fish was found.


2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Lucie Plhalova ◽  
Stanislava Stepanova ◽  
Eva Praskova ◽  
Lucie Chromcova ◽  
Lenka Zelnickova ◽  
...  

The aim of this study was to assess the impact of metribuzin in surface waters on fish under experimental conditions. The effects of subchronic exposure to metribuzin on fish growth and the development of histopathological changes in selected organs (gill, kidney, liver) and on activity of some biochemical markers (CYP450, EROD) inDanio reriowere investigated during a 28-day toxicity test. Juvenile growth tests were performed onD. rerioaccording to OECD method number 215. Fish at an initial age of 30 days were exposed to a range of metribuzin concentrations (1.5, 5, 16, 33, and 53 mg L−1). Exposure to metribuzin at 53 mg L−1was associated with increased mortality. Negative effects with regard to total body weight, length, and the inhibition of specific growth rate were induced at concentrations of 33 and 53 mg L−1. Histopathological examination revealed pathological lesions in the liver in pesticide-exposed fish only at the highest concentration of 53 mg L−1of metribuzin. Based on the results of growth rate, biochemical markers (CYP450, EROD), and histopathological examination, the lowest observed effect concentration (LOEC) value was 33 mg L−1and the no observed effect concentration (NOEC) value was 16 mg L−1.


2009 ◽  
Vol 21 (1) ◽  
pp. 185 ◽  
Author(s):  
D. E. Kelley ◽  
J. R. Gibbons ◽  
S. E. Pratt ◽  
R. L. Smith ◽  
C. J. Mortensen

The effects of exercise on follicular development in mares have not been well established; however, a combination of exercise and heat stress can negatively affect embryo recovery rate in mares. The purpose of this research was to examine the effects of exercise, independent of heat stress, on follicular development in mares. One thoroughbred and 12 quarter horse mares, aged 3 to 15 years, were used in this study. Mares were assigned to the control group or exercised at 0700 h, 6 days a week, as follows: warmed up 5 min at a walk for 3 m s–1 and then exercised 30 min, 9 m s–1 for 10 min, 13 m s–1 for 5 min, 9 m s–1 for 5 min, 13 m s–1 for 5 min, and 9 m s–1 for 5 min. Exercised mares were given a 1-month conditioning period. Rectal temperatures were recorded immediately before and after exercise. The mean rectal temperature of mares increased by 0.85°C during exercise, whereas control mares under the same environmental conditions did not experience temperature increases. Individual mares underwent a daily transrectal ultrasound examination with a 7.5-MHz linear rectal probe to monitor follicular development. Estrous cycles were normalized to 17 days for linear follicular growth analysis. Exercise had a significant impact on follicular growth at deviation, with a greater diameter of the largest (P < 0.05) and second largest (P < 0.01) follicles in exercised v. control mares (Table 1). Additionally, growth rate of the second largest follicle was greater in the exercised group (P < 0.01). Deviation was observed in 13 of 14 cycles in control mares, whereas in exercised mares, it was clear in only 11 of 16 cycles (χ2, P = 0.086). Time of deviation was defined as the day when a difference in the growth rate between the dominant and largest subordinate follicle of a wave was first observed. The largest subordinate follicle either declined in growth rate or regressed. At Day –5 (relative to ovulation), exercised mares had significantly greater follicle diameters in both the largest and second largest follicle (P < 0.05). Exercised mares had double ovulations in 4 of 16 cycles when compared with control mares, which had double ovulations in 1 of 14 cycles. No differences were found between groups in the length of the estrous cycle or ovulatory follicular diameter. Exercised mares exhibited larger diameter follicles and an increased growth rate of the largest subordinate follicle, suggesting that exercise delayed deviation. Further research is needed to assess the impact of exercise, independent of heat stress, on fertility. Table 1.Follicle dynamics in exercised v. non-exercised mares


2012 ◽  
Vol 16 (3) ◽  
pp. 383-391 ◽  
Author(s):  
Samantha Goodman ◽  
David Hammond ◽  
Rhona Hanning ◽  
Judy Sheeshka

AbstractObjectiveCanadians consume approximately twice the daily Adequate Intake of sodium. The present study examined the efficacy of four types of front-of-package (FOP) sodium labels at influencing consumers’ selection of products low v. high in sodium.DesignParticipants were randomly assigned to one of five experimental conditions: (i) control condition with no FOP label; (ii) basic numeric FOP label; (iii) numeric FOP label with ‘high’ and ‘low’ sodium content descriptors; (iv) detailed Traffic Light (TL) label with colour coding, content descriptors and numeric information; and (v) simple TL label with no numeric information. Participants were shown pairs of grocery products that varied in sodium content and told they could choose a free sample. Selection of the low-sodium v. the high-sodium product was the primary behavioural outcome, in addition to ratings of effectiveness, understanding, liking and believability.SettingWaterloo, Ontario, Canada.SubjectsAdults (n 430) aged ≥18 years, recruited from community settings.ResultsParticipants in the three FOP conditions with ‘high/low’ sodium content descriptors were significantly more likely to choose the lower-sodium product compared with the control group. The detailed TL label was ranked most effective at helping participants select low-sodium products, and was rated significantly higher than other formats in liking, understanding and believability. Product selection did not differ significantly across sociodemographic groups.ConclusionsFOP labels that include content descriptors may be more effective in helping consumers to select lower-sodium products. TL labels, which incorporate content descriptors and colour coding, should be considered for future FOP labelling initiatives.


2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Fawzy I. Magouz ◽  
Mahmoud F.I. Salem ◽  
Aliaa E.I. Emara ◽  
Mohamed M. Hassan ◽  
Mahmoud A.O. Dawood

Abstract The feasible strategies to keep up the growth and wellbeing of aquatic organisms by using beneficial dietary additives is highly recommended. Among these additives, β-glucan (BG) and mannanoligosaccharide (MOS) were examined on common carp performances in the current experiment. For 80 days, carps fed four diets with 0%, 1%, 2%, and 3% BG/MOS (1:1) and distributed in four groups (triplicates). Higher final weight and weight gain (P < 0.05) were detected in carps fed 1%, 2%, and 3% of BG/MOS than the control group. The feed conversion ratio showed lower values (P < 0.05) in carps-fed BG/MOS mixture at 2%, and 3% than 0% and 1% levels. The activities of amylase, lipase, and protease were higher in fish fed BG/MOS mixture than the control group. Interestingly, fish fed BG/MOS mixture had markedly increased villi length and width with branched villi integrity. Further, the count of goblet cells was increased (P < 0.05) in groups fed BG/MOS mixture than carps fed the basal diet. The hemoglobin and hematocrit levels and the counts of red and white blood cells were higher in carps fed BG/MOS mixture than the control with the highest counts in 3% level. Additionally, the blood total protein, globulin, and phagocytic activity were higher (P < 0.05) in groups received BG/MOS mixture at 3% than the control. The lysozyme had a higher value in 1% group than the control (P < 0.05). Therefore, using the mixture of BG/MOS at 2-3% is suggested for improving the growth rate, intestinal health, blood health, and immunity of common carp.


2018 ◽  
Vol 18 (3) ◽  
pp. 665-696 ◽  
Author(s):  
Rafael Matielo ◽  
Roberta Pires de Oliveira ◽  
Luciane Baretta

ABSTRACT This study investigates the impact of intralingual and interlingual subtitles on Brazilian English as a Foreign Language (EFL) learners as a result of their processing of a North-American sitcom. More specifically, it examines whether subtitling interacts with one’s individual differences, working memory (WM) as the case in point. Thirty-six intermediate-level EFL learners were evenly divided into two experimental groups (intralingual subtitles and interlingual subtitles) and one control group (no subtitles). Participants’ performance was measured based on an L2 video comprehension test and an L2 vocabulary test. Participants’ performance was correlated with their scores on two WM tests. The results obtained revealed that both participants’ L2 video comprehension, as well as their L2 vocabulary test performance, did not significantly interact with their WM capacity under any of the experimental conditions. These results are discussed in light of the possible processing mechanisms employed by the participants that may account for the lack of statistically significant correlations found.


Author(s):  
I. A. Pushkaryev ◽  
T. V. Kureninova ◽  
T. L. Silivirova ◽  
N. V. Shanshin

It is known that one of the most important reasons for the birth of weak calves is the impact of adverse factors on the physiological status of the cow, which in turn affects the intrauterine and postnatal development of the fetus and newborn. The results of studying the introduction of different doses of tissue biostimulator to cows during the dry period and its effect on the development of the conformation of calves received from them have been presented in the article. The experiment was carried out in 2019 on cows of Priobsky type of Black-and-White breed and calves obtained from them. In order to conduct the experiment four groups of dry cows-analogs have been formed with 10 heads in each for 55–60 days before the expected calving at the age of III lactation and older. When selecting animals the live weight and milk productivity of cows for the previous lactation have been taken into account. The highest growth rate has been observed in calves from cows the 2nd experimental group, which exceeded the control by 24,4 % (P ≤ 0,001). The best indicators of conformation development have been also observed in calves obtained from cows of the 2nd experimental group, which were injected subcutaneously with 22,5 ml of tissue preparation. So, oblique body length have been greater by 12,6 % (P ≤ 0,001), chest girth by 17,3 % (P ≤ 0,001), width of hook bones by 21,0 % (P ≤ 0,01), the width of the pin bones by 15,0 % (P ≤ 0,05). The lengthiness index was by 11,5 % higher (P ≤ 0,001), the blockiness index was by 4,1 % higher (P ≤ 0,05), and the boniness index was by 10,6 % lower (P ≤ 0,05) than in the control group herdmates.


2005 ◽  
Vol 108-109 ◽  
pp. 445-450 ◽  
Author(s):  
Y. Bogumilowicz ◽  
J.M. Hartmann ◽  
F. Laugier ◽  
G. Rolland ◽  
Thierry Billon

We have focused in this paper on the impact of the growth rate and of the grading rate on the structural properties of Si0.8Ge0.2 virtual substrates grown at 900°C in a commercial reduced pressure chemical vapour deposition reactor. Adopting a grading rate of 4% Ge / $m together with a growth rate around 140 nm min.-1 yields very high quality Si0.8Ge0.2 virtual substrates. Their macroscopic degree of strain relaxation is indeed very close to 100%, their surface root mean square roughness is around 2.3 nm and most importantly their field threading dislocation density is of the order of 6x104 cm-2 only, with almost no pile-ups.


2018 ◽  
Vol 42 (3) ◽  
pp. 297-306 ◽  
Author(s):  
Andrzej Gugołek ◽  
Jerzy Juśkiewicz ◽  
Dorota Kowalska ◽  
Cezary Zwoliński ◽  
Przemysław Sobiech ◽  
...  

ABSTRACT In recent years, a trend has emerged to eliminate soybean meal (SBM) from rabbit diets. It was hypothesized that a dietary mixture composed of rapeseed meal (RSM), white lupine seeds (WLS) and pea seeds (PS) could be a substitute for SBM in diets for growing rabbits without compromising their growth and physiological parameters. To verify this hypothesis, selected parameters describing the growth rate of rabbits, their blood and slaughter parameters and caecum function were analyzed. The experiment was performed on ninety HYPLUS rabbits. Control group (C) animals were fed a diet containing 15% SBM. The diet administered to the first experimental group (E1) contained 7.5% SBM, 5.0% RSM, 4.0% WLS and 3.0% PS. In the diet fed to the second experimental group (E2), SBM was completely replaced with RSM, WLS and PS. The substitution did not exert a significant negative effect on the growth rate of rabbits, selected morphological and biochemical blood parameters or carcass characteristics. The comparable growth parameters of control group rabbits and rabbits fed diets containing SBM substitutes could be partially attributed to beneficial changes in the enzymatic activity of caecal microbiota, which have a positive influence on fermentation processes in the lower gastrointestinal tract. The results of this study indicate that SBM can be completely replaced with a mixture of RSM, WLS and PS in growing rabbit diets.


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