scholarly journals Microbe Profile: Komagataella phaffii: a methanol devouring biotech yeast formerly known as Pichia pastoris

Microbiology ◽  
2020 ◽  
Vol 166 (7) ◽  
pp. 614-616 ◽  
Author(s):  
Lina Heistinger ◽  
Brigitte Gasser ◽  
Diethard Mattanovich
Keyword(s):  
Energy Source ◽  
Pichia Pastoris ◽  
Secondary Metabolism ◽  
Mating Type ◽  
Heterologous Proteins ◽  
Methylotrophic Yeasts ◽  
Komagataella Phaffii ◽  
Primary And Secondary Metabolism ◽  
Diploid Cells

Methylotrophic yeasts of the genus Komagataella are abundantly found in tree exudates. Their ability to utilize methanol as carbon and energy source relies on an assimilation pathway localized in largely expanded peroxisomes, and a cytosolic methanol dissimilation pathway. Other substrates like glucose or glycerol are readily utilized as well. Komagataella yeasts usually grow as haploid cells and are secondary homothallic as they can switch mating type. Upon mating diploid cells sporulate readily, forming asci with four haploid spores. Their ability to secrete high amounts of heterologous proteins made them interesting for biotechnology, which expands today also to other products of primary and secondary metabolism.

Carbon source requirements for mating and mating‐type switching in the methylotrophic yeasts Ogataea (Hansenula) polymorpha and Komagataella phaffii (Pichia pastoris)

Yeast ◽  
2020 ◽  
Vol 37 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Dahao Feng ◽  
Anton Stoyanov ◽  
Juliana C. Olliff ◽  
Kenneth H. Wolfe ◽  
Kantcho Lahtchev ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Carbon Source ◽  
Mating Type ◽  
Hansenula Polymorpha ◽  
Methylotrophic Yeasts ◽  
Komagataella Phaffii ◽  
Mating Type Switching

scholarly journals Yeast Methylotrophy: Metabolism, Gene Regulation and Peroxisome Homeostasis

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Hiroya Yurimoto ◽  
Masahide Oku ◽  
Yasuyoshi Sakai
Keyword(s):  
Energy Source ◽  
Molecular Basis ◽  
Yeast Species ◽  
Model Organisms ◽  
Heterologous Proteins ◽  
Peroxisome Biogenesis ◽  
Methylotrophic Yeasts ◽  
Methanol Metabolism ◽  
Membrane Bound ◽  
Metabolism Gene

Eukaryotic methylotrophs, which are able to obtain all the carbon and energy needed for growth from methanol, are restricted to a limited number of yeast species. When these yeasts are grown on methanol as the sole carbon and energy source, the enzymes involved in methanol metabolism are strongly induced, and the membrane-bound organelles, peroxisomes, which contain key enzymes of methanol metabolism, proliferate massively. These features have made methylotrophic yeasts attractive hosts for the production of heterologous proteins and useful model organisms for the study of peroxisome biogenesis and degradation. In this paper, we describe recent insights into the molecular basis of yeast methylotrophy.

scholarly journals Mating Type in Chlamydomonas Is Specified by mid, the Minus-Dominance Gene

Genetics ◽  
1997 ◽  
Vol 146 (3) ◽  
pp. 859-869 ◽  
Author(s):  
Patrick J Ferris ◽  
Ursula W Goodenough
Keyword(s):  
Transcription Factor ◽  
Mating Type ◽  
Codon Bias ◽  
Leucine Zipper ◽  
Laboratory Strain ◽  
Leucine Zipper Motif ◽  
Type Locus ◽  
Diploid Cells ◽  
Necessary And Sufficient

Diploid cells of Chlamydomonas reinhardtii that are heterozygous at the mating-type locus (mt  +/mt  –) differentiate as minus gametes, a phenomenon known as minus dominance. We report the cloning and characterization of a gene that is necessary and sufficient to exert this minus dominance over the plus differentiation program. The gene, called mid, is located in the rearranged (R) domain of the mt  – locus, and has duplicated and transposed to an autosome in a laboratory strain. The imp11 mt  – mutant, which differentiates as a fusion-incompetent plus gamete, carries a point mutation in mid. Like the fus1 gene in the mt  + locus, mid displays low codon bias compared with other nuclear genes. The mid sequence carries a putative leucine zipper motif, suggesting that it functions as a transcription factor to switch on the minus program and switch off the plus program of gametic differentiation. This is the first sex-determination gene to be characterized in a green organism.

scholarly journals Metabolite profiling during graft union formation reveals the reprogramming of primary metabolism and the induction of stilbene synthesis at the graft interface in grapevine

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Duyên Prodhomme ◽  
Josep Valls Fonayet ◽  
Cyril Hévin ◽  
Céline Franc ◽  
Ghislaine Hilbert ◽  
...  
Keyword(s):  
Amino Acids ◽  
Secondary Metabolism ◽  
Fruits And Vegetables ◽  
Cell Formation ◽  
Union Formation ◽  
Primary Metabolism ◽  
Graft Union ◽  
Primary And Secondary Metabolites ◽  
Primary And Secondary Metabolism ◽  
Graft Interface

Abstract Background Grafting with rootstocks is essential for the culture of many perennial fruit crops and is increasing being used in the production of annual fruits and vegetables. Our previous work based on microarrays showed that transcripts encoding enzymes of both primary and secondary metabolism were differentially expressed during graft union formation in both homo-grafts (a genotype grafted with itself) and hetero-grafts (two different genotypes grafted together). The aim of this study was to profile primary and secondary metabolites, and quantify the activity of phenylalanine ammonia lyase (PAL) and neutral invertase (NI) in the scion and rootstock tissues and the graft interface of homo and hetero-grafts of grapevine 1 month after grafting. Table-top grafting was done on over-wintering stems (canes) of grapevine and the graft interface tissues (containing some woody stem tissues and callus) were compared to the surrounding rootstock and scion tissues. The objective was to identify compounds involved in graft union formation and hetero-grafting responses. Results A total of 54 compounds from primary and secondary metabolism (19 amino acids, five primary and 30 secondary compounds metabolites) and the activity of two enzymes were measured. The graft interface was associated with an increase in the accumulation of the branched-chain amino acids, basic amino acids, certain stilbene compounds and higher PAL and NI activity in comparison to the surrounding woody stem tissues. Some amino acids and stilbenes were identified as being accumulated differently between the graft interfaces of the scion/rootstock combinations in a manner which was unrelated to their concentrations in the surrounding woody stem tissues. Conclusions This study revealed the modification of primary metabolism to support callus cell formation and the stimulation of stilbene synthesis at the graft interface, and how these processes are modified by hetero-grafting. Knowledge of the metabolites and/or enzymes required for successful graft union formation offer us the potential to identify markers that could be used by nurseries and researchers for selection and breeding purposes.

scholarly journals Control of sulfur partitioning between primary and secondary metabolism in Arabidopsis

2012 ◽  
Vol 3 ◽  
Author(s):  
Stanislav Kopriva ◽  
Sarah G. Mugford ◽  
Patrycja Baraniecka ◽  
Bok-Rye Lee ◽  
Colette A. Matthewman ◽  
...  
Keyword(s):  
Secondary Metabolism ◽  
Primary And Secondary Metabolism

Production of foreign proteins in the yeast Pichia pastoris

1995 ◽  
Vol 73 (S1) ◽  
pp. 891-897 ◽  
Author(s):  
James M. Cregg ◽  
David R. Higgins
Keyword(s):  
Gene Expression ◽  
Pichia Pastoris ◽  
Heterologous Gene Expression ◽  
Expression System ◽  
Methylotrophic Yeast ◽  
Culture Broth ◽  
Heterologous Gene ◽  
Foreign Gene ◽  
Heterologous Proteins ◽  
Yeast Pichia Pastoris

The methanol-utilizing yeast Pichia pastoris has been developed as a host system for the production of heterologous proteins of commercial interest. An industrial yeast selected for efficient growth on methanol for biomass generation, P. pastoris is readily grown on defined medium in continuous culture at high volume and density. A unique feature of the expression system is the promoter employed to drive heterologous gene expression, which is derived from the methanol-regulated alcohol oxidase I gene (AOX1) of P. pastoris, one of the most efficient and tightly regulated promoters known. The strength of the AOX1 promoter results in high expression levels in strains harboring only a single integrated copy of a foreign-gene expression cassette. Levels may often be further enhanced through the integration of multiple cassette copies into the P. pastoris genome and strategies to construct and select multicopy cassette strains have been devised. The system is particularly attractive for the secretion of foreign-gene products. Because P. pastoris endogenous protein secretion levels are low, foreign secreted proteins often appear to be virtually the only proteins in the culture broth, a major advantage in processing and purification. Key words: heterologous gene expression, methylotrophic yeast, Pichia pastoris, secretion, glycosylation.

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