scholarly journals Evidence of hypervirulence in Listeria monocytogenes clonal complex 14

2019 ◽  
Vol 68 (11) ◽  
pp. 1677-1685
Author(s):  
Maria X. Cardenas-Alvarez ◽  
Megan K. Townsend Ramsett ◽  
Sahar Malekmohammadi ◽  
Teresa M. Bergholz
Keyword(s):  
Listeria Monocytogenes ◽  
Clonal Complex

scholarly journals Retrospective Study of Listeria Monocytogenes Isolated in the Territory of Inner Eurasia from 1947 to 1999

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 184 ◽  
Author(s):  
Psareva ◽  
Egorova ◽  
Liskova ◽  
Razheva ◽  
Gladkova ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Listeria Monocytogenes ◽  
20Th Century ◽  
Multilocus Sequence Typing ◽  
Clonal Complex ◽  
Small Ruminants ◽  
Virulence Gene ◽  
Penicillin G ◽  
Allelic Variants ◽  
Mlst Scheme

Listeriosis is one of the most significant humans and animals foodborne infectious diseases. Here, we characterized 48 Listeria monocytogenes strains isolated in the territory of inner Eurasia during the second half of the 20th century. A total of 23 strains (52.3%) were susceptible to the nine antibiotics tested, 30.43%, 15.22%, and 8.7% were resistant penicillin G, ampicillin, and enrofloxacin, respectively. We applied the multilocus sequence typing (MLST) scheme to determine the phylogenetic positions of the strains. All but one strain belonged to the II phylogenetic lineage, and the majority of the strains belonged to one of the previously described clonal complexes (СCs). More than 60% of the strains belonged to the clonal complex CC7 that prevailed among all sources, including cattle (58%), small ruminants (64%), rodents (71%), and humans (50%). Further, CC7, CC101, and CC124 were found among human isolates. The MLST scheme was supplemented with virulence gene analysis. In total, eight inlA, six inlB, and six inlC allelic variants were found, and all but one strain carried one of the two inlE alleles. Most strains (62.5%) belonged to the same multivirulence locus sequence typing (MvLST) type, which includes CC7, inlA allele 4, inlB allele 14, inlC allele 6, and inlE allele 8.

scholarly journals Draft Genome Sequences of Two Historical Listeria monocytogenes Strains from Human Listeriosis Cases in 1933

2016 ◽  
Vol 4 (6) ◽  
Author(s):  
Vikrant Dutta ◽  
Sangmi Lee ◽  
Todd J. Ward ◽  
Nathane Orwig ◽  
Eric Altermann ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
North America ◽  
Clonal Complex ◽  
Draft Genome ◽  
Genome Sequences

We report here the draft genome sequences of two Listeria monocytogenes strains from some of the earliest reported cases of human listeriosis in North America. The strains were isolated in 1933 from patients in Massachusetts and Connecticut, USA, and belong to the widely disseminated hypervirulent clonal complex 1 (CC1) and CC2.

scholarly journals Complete Genome Sequence of a Shiga Toxin-Producing Escherichia coli O26:H11 Strain (Sequence Type 21) and Two Draft Genome Sequences of Listeria monocytogenes Strains (Clonal Complex 1 [CC1] and CC59) Isolated from Fresh Produce in Germany

2020 ◽  
Vol 9 (49) ◽  
Author(s):  
Gregor Fiedler ◽  
Jan Kabisch ◽  
Erik Brinks ◽  
Sabrina Sprotte ◽  
Christina Boehnlein ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Shiga Toxin ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Clonal Complex ◽  
Draft Genome ◽  
Sequence Type ◽  
Genome Sequences ◽  
Content Type

ABSTRACT The complete genome sequence of a Shiga toxin-producing Escherichia coli (STEC) O26:H11 strain, MBT-5 (sequence type 21 [ST21], stx1a, stx2a, eae, ehxA), and two draft genome sequences of Listeria monocytogenes strains MBT-6 and MBT-7 belonging to the virulent sequence types 1 (ST1, clonal complex 1 [CC1]) and 59 (ST59, CC59), respectively, were determined. The strains were isolated in 2015 from ready-to-eat mixed greens in Germany.

scholarly journals Characterization and Genetic Diversity of Listeria monocytogenes Isolated from Cattle Abortions in Latvia, 2013–2018

2021 ◽  
Vol 8 (9) ◽  
pp. 195
Author(s):  
Žanete Šteingolde ◽  
Irēna Meistere ◽  
Jeļena Avsejenko ◽  
Juris Ķibilds ◽  
Ieva Bergšpica ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Listeria Monocytogenes ◽  
Virulence Factors ◽  
Causative Agent ◽  
Animal Species ◽  
Clonal Complex ◽  
The Third ◽  
Wide Range ◽  
Sequence Types

Listeria monocytogenes can cause disease in humans and in a wide range of animal species, especially in farm ruminants. The aim of the study was to determine the prevalence and genetic diversity of L. monocytogenes related to 1185 cattle abortion cases in Latvia during 2013–2018. The prevalence of L. monocytogenes among cattle abortions was 16.1% (191/1185). The seasonality of L. monocytogenes abortions was observed with significantly higher occurrence (p < 0.01) in spring (March–May). In 61.0% of the cases, the affected cattle were under four years of age. L. monocytogenes abortions were observed during the third (64.6%) and second (33.3%) trimesters of gestation. Overall, 27 different sequence types (ST) were detected, and four of them, ST29 (clonal complex, CC29), ST37 (CC37), ST451 (CC11) and ST7 (CC7), covered more than half of the L. monocytogenes isolates. Key virulence factors like the prfA-dependent virulence cluster and inlA, inlB were observed in all the analyzed isolates, but lntA, inlF, inlJ, vip were associated with individual sequence types. Our results confirmed that L. monocytogenes is the most important causative agent of cattle abortions in Latvia and more than 20 different STs were observed in L. monocytogenes abortions in cattle.

scholarly journals Emergence and global spread of Listeria monocytogenes main clinical clonal complex

2021 ◽  
Vol 7 (49) ◽  
Author(s):  
Alexandra Moura ◽  
Noémie Lefrancq ◽  
Thierry Wirth ◽  
Alexandre Leclercq ◽  
Vítor Borges ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Clonal Complex ◽  
Global Spread

scholarly journals Emergence and global spread of Listeria monocytogenes main clinical clonal complex

2020 ◽  
Author(s):  
Alexandra Moura ◽  
Noémie Lefrancq ◽  
Alexandre Leclercq ◽  
Thierry Wirth ◽  
Vítor Borges ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Disease Surveillance ◽  
Dairy Products ◽  
Evolutionary History ◽  
Industrial Revolution ◽  
Clonal Complex ◽  
Local Level ◽  
Foodborne Pathogen ◽  
Global Spread ◽  
Insight Into

AbstractRetracing microbial emergence and spread is essential to understanding the evolution and dynamics of pathogens. The bacterial foodborne pathogen Listeria monocytogenes clonal complex 1 (Lm-CC1) is the most prevalent clonal group associated with listeriosis, and is strongly associated with cattle and dairy products. Here we analysed 2,021 Lm-CC1 isolates collected from 40 countries, since the first Lm isolation to the present day, to define its evolutionary history and population dynamics. Our results suggest that Lm-CC1 spread worldwide from North America following the Industrial Revolution through two waves of expansion, coinciding with the transatlantic livestock trade in the second half of the 19th century and the rapid growth of cattle farming in the 20th century. Lm-CC1 then firmly established at a local level, with limited inter-country spread. This study provides an unprecedented insight into Lm-CC1 phylogeography and dynamics and can contribute to effective disease surveillance to reduce the burden of listeriosis.

scholarly journals Ruminant Rhombencephalitis-Associated Listeria monocytogenes Alleles Linked to a Multilocus Variable-Number Tandem-Repeat Analysis Complex

2011 ◽  
Vol 77 (23) ◽  
pp. 8325-8335 ◽  
Author(s):  
Lina Balandyté ◽  
Isabelle Brodard ◽  
Joachim Frey ◽  
Anna Oevermann ◽  
Carlos Abril
Keyword(s):  
Listeria Monocytogenes ◽  
Tandem Repeat ◽  
Genetic Relatedness ◽  
Clonal Complex ◽  
Variable Number Tandem Repeat ◽  
Virulence Gene ◽  
Variable Number ◽  
Content Type ◽  
Repeat Analysis ◽  
Cns Infections

ABSTRACTListeria monocytogenesis among the most important food-borne pathogens and is well adapted to persist in the environment. To gain insight into the genetic relatedness and potential virulence ofL. monocytogenesstrains causing central nervous system (CNS) infections, we used multilocus variable-number tandem-repeat analysis (MLVA) to subtype 183L. monocytogenesisolates, most from ruminant rhombencephalitis and some from human patients, food, and the environment. Allelic-profile-based comparisons groupedL. monocytogenesstrains mainly into three clonal complexes and linked single-locus variants (SLVs). Clonal complex A essentially consisted of isolates from human and ruminant brain samples. All but one rhombencephalitis isolate from cattle were located in clonal complex A. In contrast, food and environmental isolates mainly clustered into clonal complex C, and none was classified as clonal complex A. Isolates of the two main clonal complexes (A and C) obtained by MLVA were analyzed by PCR for the presence of 11 virulence-associated genes (prfA,actA,inlA,inlB,inlC,inlD,inlE,inlF,inlG,inlJ, andinlC2H). Virulence gene analysis revealed significant differences in theactA,inlF,inlG, andinlJallelic profiles between clinical isolates (complex A) and nonclinical isolates (complex C). The association of particular alleles ofactA,inlF, and newly described alleles ofinlJwith isolates from CNS infections (particularly rhombencephalitis) suggests that these virulence genes participate in neurovirulence ofL. monocytogenes. The overall absence ofinlGin clinical complex A and its presence in complex C isolates suggests that the InlG protein is more relevant for the survival ofL. monocytogenesin the environment.

scholarly journals Retrospective Use of Whole-Genome Sequencing Expands the Multicountry Outbreak Cluster of Listeria monocytogenes ST1247

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Mihkel Mäesaar ◽  
Rafael Mamede ◽  
Terje Elias ◽  
Mati Roasto
Keyword(s):  
Listeria Monocytogenes ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Core Genome ◽  
Clonal Complex ◽  
Antibiotic Resistance Genes ◽  
Whole Genome ◽  
Outbreak Strain ◽  
Determining Factors ◽  
Fish Product

Listeria monocytogenes sequence type 1247 clonal complex 8 caused a prolonged multicountry outbreak in five EU countries: Denmark, Estonia, Finland, France, and Sweden. A total of 22 disease cases were identified with onset of symptoms between July 2014 and February 2019. Five patients died due to, or with, the disease. The retrospective analysis of L. monocytogenes isolate VLTRLM2013 revealed the presence of an outbreak-related strain (cgMLST type L2-SL8-ST1247-CT4158) in ready-to-eat fish product more than a year prior to the first outbreak-related cases. Reference outbreak strain and VLTRLM2013 strain were compared using core genome and whole-genome multilocus sequence typing analyses. Genomic level differences of the persistent L. monocytogenes strains associated with a prolonged multicountry foodborne listeriosis outbreak are described. It was concluded that the persistent nature of the multicountry outbreak-related L. monocytogenes strain VLTRLM2013 together with stress island, virulence, and antibiotic resistance genes could potentially be the determining factors for the extensive and prolonged outbreak affecting five European Union countries. Our results support the systematic application of whole-genome sequencing in food and public health surveillance and further encourages its wide adoption.

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