Using statistical properties of GB-speckles coding the nucleotide sequences of the 'housekeeping' genes of Listeria monocytogenes for characterization of differences between ST7 and ST106 of the clonal complex СС7

2020 ◽  
Author(s):  
Onega V. Ulianova ◽  
Sergey S. Zaytsev ◽  
Alexander S. Ulyanov ◽  
Yury V. Saltykov ◽  
Sergey S. Ulyanov ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Clonal Complex ◽  
Housekeeping Genes ◽  
Nucleotide Sequences ◽  
Statistical Properties

scholarly journals Diversity unearthed by the estimated molecular phylogeny and ecologically quantitative characteristics of uncultured Ehrlichia bacteria in Haemaphysalis ticks, Japan

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Hongru Su ◽  
Eri Onoda ◽  
Hitoshi Tai ◽  
Hiromi Fujita ◽  
Shigetoshi Sakabe ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
16S Rrna ◽  
Core Genome ◽  
Phylogenetic Analyses ◽  
Taxonomic Status ◽  
Housekeeping Genes ◽  
Pcr Screening ◽  
Taxonomic Profiling ◽  
Quantitative Characteristics

AbstractEhrlichia species are obligatory intracellular bacteria transmitted by arthropods, and some of these species cause febrile diseases in humans and livestock. Genome sequencing has only been performed with cultured Ehrlichia species, and the taxonomic status of such ehrlichiae has been estimated by core genome-based phylogenetic analysis. However, many uncultured ehrlichiae exist in nature throughout the world, including Japan. This study aimed to conduct a molecular-based taxonomic and ecological characterization of uncultured Ehrlichia species or genotypes from ticks in Japan. We first surveyed 616 Haemaphysalis ticks by p28-PCR screening and analyzed five additional housekeeping genes (16S rRNA, groEL, gltA, ftsZ, and rpoB) from 11 p28-PCR-positive ticks. Phylogenetic analyses of the respective genes showed similar trees but with some differences. Furthermore, we found that V1 in the V1–V9 regions of Ehrlichia 16S rRNA exhibited the greatest variability. From an ecological viewpoint, the amounts of ehrlichiae in a single tick were found to equal approx. 6.3E+3 to 2.0E+6. Subsequently, core-partial-RGGFR-based phylogenetic analysis based on the concatenated sequences of the five housekeeping loci revealed six Ehrlichia genotypes, which included potentially new Ehrlichia species. Thus, our approach contributes to the taxonomic profiling and ecological quantitative analysis of uncultured or unidentified Ehrlichia species or genotypes worldwide.

scholarly journals Physiological and Transcriptional Characterization of Persistent and Nonpersistent Listeria monocytogenes Isolates

2011 ◽  
Vol 77 (18) ◽  
pp. 6559-6569 ◽  
Author(s):  
Edward M. Fox ◽  
Nola Leonard ◽  
Kieran Jordan
Keyword(s):  
Listeria Monocytogenes ◽  
Antimicrobial Agents ◽  
Cetylpyridinium Chloride ◽  
Phenotype Microarray ◽  
Content Type ◽  
Benzethonium Chloride ◽  
Transcriptomic Sequencing ◽  
Ammonium Compounds ◽  
Compare Gene Expression

ABSTRACTThis study aimed to characterize physiological differences between persistent and presumed nonpersistentListeria monocytogenesstrains isolated at processing facilities and to investigate the molecular basis for this by transcriptomic sequencing. Full metabolic profiles of two strains, one persistent and one nonpersistent, were initially screened using Biolog's Phenotype MicroArray (PM) technology. Based on these results, in which major differences from selected antimicrobial agents were detected, another persistent strain and two nonpersistent strains were characterized using two antimicrobial PMs. Resistance to quaternary ammonium compounds (QACs) was shown to be higher among persistent strains. Growth of persistent and nonpersistent strains in various concentrations of the QACs benzethonium chloride (BZT) and cetylpyridinium chloride (CPC) was determined. Transcriptomic sequencing of a persistent and a presumed nonpersistent strain was performed to compare gene expression among these strains in the presence and absence of BZT. Two strains, designated “frequent persisters” because they were the most frequently isolated at the processing facility, showed overall higher resistance to QACs. Transcriptome analysis showed that BZT induced a complex peptidoglycan (PG) biosynthesis response, which may play a key role in BZT resistance. Comparison of persistent and nonpersistent strains indicated that transcription of many genes was upregulated among persistent strains. This included three gene operons:pdu,cob-cbi, andeut. These genes may play a role in the persistence ofL. monocytogenesoutside the human host.

scholarly journals Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

2015 ◽  
Vol 45 (12) ◽  
pp. 2197-2200 ◽  
Author(s):  
Thor Vinícius Martins Fajardo ◽  
Monique Bezerra Nascimento ◽  
Marcelo Eiras ◽  
Osmar Nickel ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Amino Acid ◽  
Molecular Characterization ◽  
Molecular Analysis ◽  
Nucleotide Sequences ◽  
Amino Acid Sequences ◽  
Causal Agent ◽  
Ringspot Virus ◽  
Prunus Necrotic Ringspot Virus ◽  
First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

scholarly journals Molecular characterization of Listeria monocytogenes isolates from a small-scale meat processor in Montenegro, 2011–2014

2019 ◽  
Vol 79 ◽  
pp. 116-122 ◽  
Author(s):  
Ivana Zuber ◽  
Brankica Lakicevic ◽  
Ariane Pietzka ◽  
Dubravka Milanov ◽  
Vesna Djordjevic ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Molecular Characterization ◽  
Small Scale

scholarly journals Prevalence of Listeria spp. and characterization of Listeria monocytogenes isolated from food products in Tetouan, Morocco

Food Control ◽  
2018 ◽  
Vol 84 ◽  
pp. 436-441 ◽  
Author(s):  
Nadia Amajoud ◽  
Alexandre Leclercq ◽  
Jose M. Soriano ◽  
Hélène Bracq-Dieye ◽  
Mohammed El Maadoudi ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Products

scholarly journals Use of multi-locus sequencing typing as identification method for the food-borne pathogen Listeria monocytogenes: a review

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Sonia Lamon ◽  
Domenico Meloni ◽  
Simonetta Gianna Consolati ◽  
Anna Mureddu ◽  
Rina Mazzette
Keyword(s):  
Listeria Monocytogenes ◽  
Detection Methods ◽  
Causative Organism ◽  
Intracellular Pathogen ◽  
Bacterial Strains ◽  
New Approach ◽  
Food Borne ◽  
Global Epidemiology ◽  
Selection Of

<em>Listeria monocytogenes</em> is an ubiquitous, intracellular pathogen which has been implicated within the past decade as the causative organism in several outbreaks of foodborne diseases. In this review, a new approach to molecular typing primarily designed for global epidemiology has been described: multi-<em>locus</em> sequencing typing (MLST). This approach is novel, in that it uses data that allow the unambiguous characterization of bacterial strains via the Internet. Our aim is to present the currently available selection of references on <em>L. monocytogenes</em> MLST detection methods and to discuss its use as <em>gold</em> <em>standard</em> to <em>L. monocytogenes</em> subtyping method.

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