scholarly journals Noviherbaspirillum aerium sp. nov., isolated from air

2020 ◽  
Vol 70 (12) ◽  
pp. 6390-6395 ◽  
Author(s):  
Han Xue ◽  
Chun-gen Piao ◽  
Ying-hua Lin ◽  
Dan-ran Bian ◽  
Yong Li

A Gram-stain-negative, strictly aerobic, non-spore-forming, rod-shaped, motile with polar flagella and pale-orange bacterium, designated strain 122213-3T, was isolated from air, collected at the foot of the Xiangshan Mountain, located in Beijing, PR China. Optimal growth occurred at 28 °C, at pH 7 and in the presence of 0–1 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that 122213-3T clustered with species of the genus Noviherbaspirillum and formed a distinct sublineage, showing highest similarities to Noviherbaspirillum malthae CC-AFH3T (96.88 %), Noviherbaspirillum massiliense JC206T (95.78 %) and Noviherbaspirillum aurantiacum SUEMI08T (95.78 %). The predominant cellular fatty acids were summed feature 3 (C16 : 1  ω6c and/or C16 : 1  ω7c), summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c) and C16 : 0. The predominant quinone was ubiquinone 8 (Q-8). The polar lipids comprised phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid and two unidentified polar lipids. The polyamine pattern showed the presence of putrescine as the major polyamine, with minor amounts of 2-hydroxyputrescine. The DNA G+C content was 60.1 mol%. The phylogenetic analysis and physiological and biochemical data showed that strain 122213-3T should be classified as representing a novel species in the genus Noviherbaspirillum , for which the name Noviherbaspirillum aerium sp. nov. is proposed. The type strain of N. aerium is 122213-3T (=CFCC 14286T=LMG 30131T).

2020 ◽  
Vol 70 (11) ◽  
pp. 5943-5949 ◽  
Author(s):  
Yun-zhen Yang ◽  
Ji-feng Chen ◽  
Wan-ru Huang ◽  
Ran-ran Zhang ◽  
Shuangjiang Liu ◽  
...  

A novel Gram-stain-negative, strictly aerobic, rod-shaped, brick red-pigmented bacterium, designated R-22-1 c-1T, was isolated from water from Baiyang Lake, Hebei Province, PR China. The strain was able to grow at 20–30 °C (optimum, 30 °C) and pH 6–7 (optimum, pH 6) in Reasoner’s 2A medium. 16S rRNA gene sequence and phylogenetic analyses of R-22-1 c-1T revealed closest relationships to Rufibacter immobilis MCC P1T (97.8 %), Rufibacter sediminis H-1T (97.9 %) and Rufibacter glacialis MDT1-10-3T (97.0 %), with other species of the genus Rufibacter showing less than 97.0 % sequence similarity. The predominant polar lipids were phosphatidylethanolamine, two unidentified aminophospholipids and three unidentified lipids. The major cellular fatty acids were iso-C15 : 0, C15 : 1  ω6c, C17 : 1  ω6c, anteiso-C15 : 0, summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1  ω7c and/or C16 : 1  ω6c) and summed feature 4 (iso-C17 : 1I and/or anteiso-C17 : 1B). The respiratory quinone was MK-7. The draft genome of R-22-1 c-1T was 5.6 Mbp in size, with a G+C content of 50.2 mol%. The average nucleotide identity and digital DNA–DNA hybridization relatedness values between strain R-22-1 c-1T and related type strains were R. immobilis MCC P1T (77.2 and 21.8 %), R. sediminis H-1T (81.6 and 21.4 %) and R. tibetensis 1351T (78.5 and 22.9 %). Based on these phylogenetic, chemotaxonomic and genotypic results, strain R-22-1 c-1T represents a novel species in the genus Rufibacter , for which the name Rufibacter latericius sp. nov. is proposed. The type strain is R-22-1 c-1T (=CGMCC 1.13570T=KCTC 62781T).


2012 ◽  
Vol 62 (Pt_8) ◽  
pp. 1850-1856 ◽  
Author(s):  
Herbert Seiler ◽  
Anne Bleicher ◽  
Hans-Jürgen Busse ◽  
Josef Hüfner ◽  
Siegfried Scherer

A novel halophilic, Gram-reaction-negative, strictly aerobic, non-motile, rod-shaped and oxidase- and catalase-positive bacterial strain, designated WCC 4520T, was isolated from a semi-hard, Raclette-type cheese. The colonies were yellow–orange; flexirubin-type pigments were not found. The strain hydrolysed gelatin, hippurate, tyrosine and Tweens 20 and 80. Optimal growth was observed with 6–8 % (w/v) NaCl, at pH 7–8 and at 27–30 °C. The genomic DNA G+C content was 33.6 mol%. In phylogenetic analysis based on 16S rRNA gene sequences, strain WCC 4520T appeared to be a member of the family Flavobacteriaceae and the closest phylogenetic neighbours were identified as Psychroflexus gondwanensis DSM 5423T (94.0 % 16S rRNA gene sequence similarity) and Psychroflexus salinarum CCUG 56752T (94.0 %). The predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C15 : 1 G and iso-C17 : 0 3-OH. The only detected quinone was MK-6 and the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and an unidentified polar lipid. Minor polar lipids and traces of polyamines were also detected. On the basis of the data presented, strain WCC 4520T represents a novel species of the genus Psychroflexus , for which the name Psychroflexus halocasei sp. nov. is proposed. The type strain is WCC 4520T ( = LMG 25857T = CCUG 59705T).


2014 ◽  
Vol 64 (Pt_11) ◽  
pp. 3897-3903 ◽  
Author(s):  
Asif Hameed ◽  
Mariyam Shahina ◽  
Shih-Yao Lin ◽  
Wei-An Lai ◽  
You-Cheng Liu ◽  
...  

A Gram-stain-negative, strictly aerobic, rod- or oval-shaped, motile, non-spore-forming bacterium, designated strain CC-AMHB-3T, was isolated from coastal surface seawater off Hualien, Taiwan. The novel strain showed high pairwise 16S rRNA gene sequence similarities to Ruegeria mobilis NBRC 101030T (96.5 %), Ruegeria scottomollicae LMG 24367T (96.4 %), Phaeobacter aquaemixtae SSK6-1T (96.2 %), Phaeobacter daeponensis TF-218T (96.2 %), Cribrihabitans marinus CZ-AM5T (96.1 %) and other species of the family Rhodobacteraceae (≤95.9 %). However, strain CC-AMHB-3T formed a distinct phyletic lineage associated with C. marinus CZ-AM5T during phylogenetic analyses. The polar lipid profile of strain CC-AMHB-3T included major amounts of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylcholine; moderate amounts of phosphatidylethanolamine, an unidentified aminolipid, an unidentified phospholipid and an unidentified lipid; and trace amounts of an unidentified lipid and an unidentified phospholipid, which was qualitatively almost in line with that of C. marinus CZ-AM5T but remarkably distinct as compared with the type species of the genera Ruegeria ( Ruegeria atlantica JCM 21234T) and Phaeobacter ( Phaeobacter gallaeciensis JCM 21319T). In line with the fatty acid profile of C. marinus CZ-AM5T, the major (>5 % of total) fatty acids of strain CC-AMHB-3T were C18 : 1ω7c and/or C18 : 1ω6c (summed feature 8), 11-methyl C18 : 1ω7c and C16 : 0. The DNA G+C content was 66.7 mol%. Ubiquinone-10 (Q-10) was the sole respiratory quinone. Thus, based on the results of the polyphasic study presented here, strain CC-AMHB-3T is considered to represent a novel species of the genus Cribrihabitans , for which the name Cribrihabitans neustonicus sp. nov. is proposed; the type strain is CC-AMHB-3T ( = JCM 19537T = BCRC 80695T). In addition, an emended description of the genus Cribrihabitans is also proposed.


2013 ◽  
Vol 63 (Pt_4) ◽  
pp. 1235-1240 ◽  
Author(s):  
Tong Yu ◽  
Qi Yin ◽  
Xiangyu Song ◽  
Rui Zhao ◽  
Xiaochong Shi ◽  
...  

A novel Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic strain with gliding motility, designated SW024T, was isolated from surface seawater of the South Pacific Gyre (26° 29′ S 137° 56′ W) during the Integrated Ocean Drilling Program Expedition 329. Phylogenetic analyses based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina muelleri KMM 6020T, Aquimarina macrocephali JAMB N27T, Aquimarina addita JC2680T, Aquimarina mytili PSC33T, Aquimarina intermedia KMM 6258T, Aquimarina latercula ATCC 23177T, Aquimarina spongiae A6T, Aquimarina agarilytica ZC1T and Aquimarina brevivitae SMK-19T (96.1, 95.5, 95.0, 94.4, 94.3, 94.0, 93.4, 93.3 and 93.2 % similarities, respectively), demonstrating that the novel strain belonged to the genus Aquimarina . The DNA G+C content of strain SW024T was 30.8 mol%. The major respiratory quinone of strain SW024T was MK-6. The dominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, C16 : 0 10-methyl and/or iso-C17 : 1ω9c, and iso-C15 : 0 3-OH. The polar lipids of strain SW024T were phosphatidylethanolamine, three unknown aminolipids and five unknown polar lipids. On the basis of combined phenotypic and phylogenetic analyses, strain SW024T represents a novel species of the genus Aquimarina , for which the name Aquimarina longa sp. nov. is proposed. The type strain is SW024T ( = CGMCC 1.11007T = JCM 17859T). An emended description of A. muelleri is also proposed.


2020 ◽  
Vol 70 (5) ◽  
pp. 3186-3193 ◽  
Author(s):  
Gui Zhang ◽  
Xin-He Lai ◽  
Jing Yang ◽  
Dong Jin ◽  
Ji Pu ◽  
...  

Two Gram-stain-negative, strictly aerobic, bright-yellow-pigmented and rod-shaped bacteria (strains 100069 and 100111T) with a single polar flagellum were isolated from the rectal contents of plateau pika (Ochotona curzoniae). Based on the results of nearly full-length 16S rRNA gene sequence and phylogenetic analyses, strains 100069 and 100111T belong to the genus Luteimonas , and are closest to Luteimonas rhizosphaerae 4-12T (98.02 % similarity), Luteimonas aestuarii B9T (97.8 %) and Luteimonas terrae THG-MD21T (97.74 %). The DNA G+C contents of these two isolates were 68.30 mol% and 68.29 mol%, respectively. The highest average nucleotide identity (ANI) value between strain 100111T and its closely related species was 83.34 %, well below the threshold of 95–96 %. The major cellular fatty acids were iso-C11 : 0, iso-C15 : 0 and iso-C17 : 1  ω9. Polar lipid content was dominated by diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. Ubiquinone-8 (Q-8) was the predominant respiratory quinone. These two isolates grew optimally at 35–37 °C, pH 7.0–8.0 and with 1.0 % (w/v) NaCl. The results of ANI analysis and other characteristics obtained from our polyphasic study showed that strains 100069 and 100111T represent a novel species in genus Luteimonas , for which the name Luteimonas chenhongjianii sp. nov. (type strain 100111T=DSM 104077T=CGMCC 1.16429T) is proposed.


2020 ◽  
Vol 70 (8) ◽  
pp. 4760-4766 ◽  
Author(s):  
Inseong Cha ◽  
Heeyoung Kang ◽  
Haneul Kim ◽  
Seokhyeon Bae ◽  
Kiseong Joh

Strain HMF4947T, isolated from the bark of a ginkgo tree, was a pale-pink coloured, Gram-stain-negative, non-motile, strictly aerobic and rod-shaped bacterium. The isolate grew optimally on Reasoner's 2A agar at 30 °C, pH 7.0 and with 0 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain HMF4947T belonged to the genus Hymenobacter and was most closely related to Hymenobacter metalli A2-91T (96.9 % sequence similarity) and Hymenobacter pomorum 9-2-1-1T (96.5 %). The average nucleotide identity and estimated DNA–DNA hybridization values between strain HMF4947T and Hymenobacter arizonensis DSM 17870T were 74.3 and 20.5 %, respectively. The major fatty acids were summed feature 3 (C16 : 1  ω7c and/or C16 : 1  ω6c), iso-C15 : 0 and C16 : 1  ω5c. The predominant isoprenoid quinone was menaquinone-7. The polar lipids comprised phosphatidylethanolamine, one unidentified aminoglycolipid, three unidentified aminophospholipids, one unidentified phospholipid, three unidentified aminolipids, two unidentified glycolipids and three unidentified polar lipids. The genomic DNA G+C content was 59.3 mol%. Thus, based on phylogenetic, phenotypic and chemotaxonomic data, strain HMF4947T represents a novel species of the genus Hymenobacter , for which the name Hymenobacter ginkgonis sp. nov. is proposed. The type strain of the species is strain HMF4947T (=KCTC 72780T=NBRC 114271T).


2014 ◽  
Vol 64 (Pt_1) ◽  
pp. 78-82 ◽  
Author(s):  
Taeyang Kwon ◽  
Kiwoon Baek ◽  
Kiyoung Lee ◽  
Ilnam Kang ◽  
Jang-Cheon Cho

A Gram-staining-negative, chemoheterotrophic, yellow-pigmented, gliding, catalase- and oxidase-positive, flexirubin-negative, strictly aerobic bacterium, designated strain IMCC9485T, was isolated from a seawater sample collected from the Arctic Ocean. Optimal growth of strain IMCC9485T was observed at 25 °C, pH 7–8 and in the presence of 1.5–2.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain IMCC9485T belonged to the genus Formosa and was closely related to Formosa algae KCTC 12364T (98.2 %) and Formosa agariphila KCTC 12365T (98.0 %). DNA–DNA relatedness between the new isolate and F. algae and F. agariphila was far lower than 70 %, which indicated that strain IMCC9485T is a novel genomic species of the genus Formosa . The major fatty acids (>10 %) were iso-C15 : 1G (13.7 %), C16 : 1ω7c and/or C16 : 1ω6c (13.4 %) and iso-C15 : 0 (12.3 %). The G+C content of the genomic DNA was 37.6 mol%. Strain IMCC9485T contained menaquinone-6 (MK-6) as the respiratory quinone and phosphatidylethanolamine, unknown aminophospholipids and unknown polar lipids as polar lipid constituents. On the basis of phylogenetic analyses and differential phenotypic characteristics, it is suggested that strain IMCC9485T ( = KACC 17484T = KCCM 42937T = NBRC 106080T) be assigned to the genus Formosa as the type strain of a novel species, for which the name Formosa arctica sp. nov. is proposed.


2014 ◽  
Vol 64 (Pt_6) ◽  
pp. 1991-1997 ◽  
Author(s):  
Zenghu Zhang ◽  
Tong Yu ◽  
Tingting Xu ◽  
Xiao-Hua Zhang

A Gram-stain-negative, rod-shaped, non-flagellated, strictly aerobic bacterium with gliding motility, designated strain SW150T, was isolated from surface seawater of the South Pacific Gyre (39° 19′ S 139° 48′ W) during the Integrated Ocean Drilling Program Expedition 329. Optimal growth occurred in the presence of 2–4 % (w/v) NaCl, at pH 7–8 and at 28–30 °C. The dominant fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH, iso-C15 : 1 G, C16 : 1ω6c and/or C16 : 1ω7c and 10-methyl C16 : 0 and/or iso-C17 : 1ω9c. The polar lipids of strain SW150T comprised phosphatidylethanolamine, three unknown polar lipids and one unknown aminolipid. The major respiratory quinone was MK-6. The DNA G+C content of strain SW150T was 33.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Aquimarina macrocephali JAMB N27T and Aquimarina muelleri KMM 6020T with 97.8 and 96.8 % sequence similarities, respectively. The estimated DNA–DNA hybridization values were 21.00±2.33 % between strain SW150T and A. macrocephali JAMB N27T and 20.60±2.32 % between strain SW150T and Aquimarina megaterium XH134T. On the basis of polyphasic analyses, strain SW150T represents a novel species of the genus Aquimarina , for which the name Aquimarina pacifica sp. nov. is proposed. The type strain is SW150T ( = JCM 18214T = CGMCC 1.12180T).


2020 ◽  
Vol 70 (8) ◽  
pp. 4610-4615 ◽  
Author(s):  
Xiao-Yu Zhang ◽  
Rui Zhang ◽  
Jia-Cheng Wang ◽  
Ting Zhang ◽  
Zong-Jun Du

A novel Gram-stain-negative, strictly aerobic, gliding and rod-shaped bacterial strain, designated strain C33T, was isolated from Yuncheng Salt Lake, Shanxi, PR China. Strain C33T grows optimally at 37 °C, pH 7.5 and 5.0 % (w/v) NaCl. Cells of strain C33T are 0.3–0.5 µm wide and 1.0–2.0 µm long, catalase-positive and oxidase-positive. The major cellular fatty acids are iso-C15 : 0 and iso-C16 : 0. The sole respiratory quinone is Q-8. The major polar lipids include phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, one unidentified aminophospholipid, one unidentified glycolipid and four unidentified lipids. The results of phylogenetic analysis based on 16S rRNA gene sequences indicate that strain C33T has the highest similarities to Wenzhouxiangella marina KCTC 42284T (97.4 %), Wenzhouxiangella sediminis XDB06T (96.5 %) and ‘Wenzhouxiangella salilacus’ MCCC 1K03442T (95.2 %). The percentage of conserved proteins and average amino acid identity values between strain C33T and its close related species are higher than the threshold for dividing genera, the average nucleotide identity and digital DNA–DNA hybridization values are well below the threshold limits for species delineation. The genomic DNA G+C content is 63.7 mol%. Based on the results of phenotypic, chemotaxonomic and phylogenetic analyses, strain C33T is considered to represent a novel species of the genus Wenzhouxiangella , for which the name Wenzhouxiangella limi sp. nov. is proposed. The type strain is C33T (=MCCC 1H00413T=KCTC 72874T).


2020 ◽  
Vol 70 (4) ◽  
pp. 2690-2695 ◽  
Author(s):  
Delong Kong ◽  
Qi Zhang ◽  
Xu Jiang ◽  
Qingyun Ma ◽  
Xiaoyan Han ◽  
...  

A Gram-stain-positive, motile, rod-shaped bacterium, designated strain LAM7113T, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, PR China. Strain LAM7113T grew optimally at pH 8.0, 30 °C and in the presence of 1.0 % NaCl (w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LAM7113T was closely related to members of the genus Paenibacillus , with the highest similarity to Paenibacillus baekrokdamisoli Back-11T (96.2 %). The genomic DNA G+C content was 43.4 mol%. The values of average nucleotide identity and DNA–DNA hybridization were 66.1 and 27.0 %, respectively, by comparing the draft genome sequences of strain LAM7113T and P. baekrokdamisoli Back-11T. Anteiso-C15 : 0 and iso-C15 : 0 were identified as the major cellular fatty acids. Menaquinone-7 was detected as the predominant respiratory quinone. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, three unidentified aminophospholipids, three unidentified glycolipids, one unidentified phospholipid and two unknown polar lipids. Based on its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM7113T is proposed to represent a novel species of the genus Paenibacillus with the name Paenibacillus solisilvae sp. nov. The type strain is LAM7113T (=CGMCC 1.16619T=JCM 32513T).


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