scholarly journals Quantitative mass spectrometry of unconventional human biological matrices

2016 ◽  
Vol 374 (2079) ◽  
pp. 20150380 ◽  
Author(s):  
Ewelina P. Dutkiewicz ◽  
Pawel L. Urban
Keyword(s):  
Mass Spectrometry ◽  
Small Molecules ◽  
Chromatographic Separation ◽  
Certified Reference Materials ◽  
Mass Spectrometric ◽  
Quantitative Mass Spectrometry ◽  
Biological Matrices ◽  
Matrix Interferences ◽  
Non Invasive ◽  
Potential Biomarkers

The development of sensitive and versatile mass spectrometric methodology has fuelled interest in the analysis of metabolites and drugs in unconventional biological specimens. Here, we discuss the analysis of eight human matrices—hair, nail, breath, saliva, tears, meibum, nasal mucus and skin excretions (including sweat)—by mass spectrometry (MS). The use of such specimens brings a number of advantages, the most important being non-invasive sampling, the limited risk of adulteration and the ability to obtain information that complements blood and urine tests. The most often studied matrices are hair, breath and saliva. This review primarily focuses on endogenous (e.g. potential biomarkers, hormones) and exogenous (e.g. drugs, environmental contaminants) small molecules. The majority of analytical methods used chromatographic separation prior to MS; however, such a hyphenated methodology greatly limits analytical throughput. On the other hand, the mass spectrometric methods that exclude chromatographic separation are fast but suffer from matrix interferences. To enable development of quantitative assays for unconventional matrices, it is desirable to standardize the protocols for the analysis of each specimen and create appropriate certified reference materials. Overcoming these challenges will make analysis of unconventional human biological matrices more common in a clinical setting. This article is part of the themed issue ‘Quantitative mass spectrometry’.

scholarly journals Rapid Mass Spectrometric Diagnosis of Bladder Cancer via Urine Carbonyl Metabolic Fingerprints

2021 ◽  
Author(s):  
Zongxiu Nie ◽  
Yuze Li ◽  
Lixia Jiang ◽  
Zhenpeng Wang ◽  
Xiaohua Cao ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Point Of Care ◽  
Low Cost ◽  
Mass Spectrometric ◽  
Detection Sensitivity ◽  
Ionization Mass ◽  
Non Invasive ◽  
Diagnosis Method ◽  
Rapid Mass ◽  
Potential Biomarkers

Abstract The diagnosis of bladder cancer (BC) is currently based on cystoscopy, which is invasive and expensive. Here, we described a non-invasive, low-cost BC diagnosis method based on a desorption, separation, and ionization mass spectrometry platform (DSI-MS) that adopts N, N- Dimethylethylenediamine (DMED) as a differential labeling reagent. The DSI-MS platform avoids the interferences from intra- and/or inter-samples, while the DMED increases detection sensitivity and distinguishes carboxyl, aldehyde, and ketone groups from untreated samples. Carbonyl metabolic fingerprints of urine from 28 BC patients and 38 controls were portrayed and significant differences of some potential biomarkers were observed. The mechanisms of the changes have been discussed. Logistic regression (LR) was applied to discriminate BC from controls and an accuracy of 87% was achieved. We believe this patient-friendly method provides a hopeful approach for BC rapid point-of-care diagnostic.

scholarly journals Mass Spectrometric Analysis of L-carnitine and its Esters: Potential Biomarkers of Disturbances in Carnitine Homeostasis

2020 ◽  
Vol 20 (5) ◽  
pp. 336-354 ◽  
Author(s):  
Judit Bene ◽  
Andras Szabo ◽  
Katalin Komlósi ◽  
Bela Melegh
Keyword(s):  
Mass Spectrometry ◽  
Animal Studies ◽  
Screening Program ◽  
Basic Research ◽  
Autism Spectrum ◽  
Mass Spectrometric ◽  
Spectrometric Analysis ◽  
Total Carnitine ◽  
Potential Biomarkers

Purpose: After a golden age of classic carnitine research three decades ago, the spread of mass spectrometry opened new perspectives and a much better understanding of the carnitine system is available nowadays. In the classic period, several human and animal studies were focused on various distinct physiological functions of this molecule and these revealed different aspects of carnitine homeostasis in normal and pathological conditions. Initially, the laboratory analyses were based on the classic or radioenzymatic assays, enabling only the determination of free and total carnitine levels and calculation of total carnitine esters’ amount without any information on the composition of the acyl groups. The introduction of mass spectrometry allowed the measurement of free carnitine along with the specific and sensitive determination of different carnitine esters. Beyond basic research, mass spectrometry study of carnitine esters was introduced into the newborn screening program because of being capable to detect more than 30 metabolic disorders simultaneously. Furthermore, mass spectrometry measurements were performed to investigate different disease states affecting carnitine homeostasis, such as diabetes, chronic renal failure, celiac disease, cardiovascular diseases, autism spectrum disorder or inflammatory bowel diseases. Results: This article will review the recent advances in the field of carnitine research with respect to mass spectrometric analyses of acyl-carnitines in normal and various pathological states. Conclusion: The growing number of publications using mass spectrometry as a tool to investigate normal physiological conditions or reveal potential biomarkers of primary and secondary carnitine deficiencies shows that this tool brought a new perspective to carnitine research.

scholarly journals Examination of salivary proteins as biomarkers of pathological conditions. Literature review

2015 ◽  
Vol 26 (1) ◽  
pp. 71-75
Keyword(s):  
Mass Spectrometry ◽  
Human Saliva ◽  
Comprehensive Analysis ◽  
Salivary Proteins ◽  
Protein Markers ◽  
Non Invasive ◽  
Pathological Conditions ◽  
Invasive Diagnostics ◽  
Different Origin ◽  
Potential Biomarkers

Saliva is universally available biofluid, easy to collect. Comprehensive analysis and identification of the proteomic content of human saliva may contribute to the understanding of oral pathophysiology and provide a foundation for the recognition of potential biomarkers of human disease. These features make it an ideal biological material for the early detection of many diseases of different origin, and enable non-invasive diagnostics. The presence of protein markers in saliva was found with usage of capillary electrophoresis and mass spectrometry.

scholarly journals Good quantification practices of flavours and fragrances by mass spectrometry

2016 ◽  
Vol 374 (2079) ◽  
pp. 20150365 ◽  
Author(s):  
Frédéric Begnaud ◽  
Alain Chaintreau
Keyword(s):  
Mass Spectrometry ◽  
Method Validation ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Complex Media ◽  
Quantitative Mass Spectrometry ◽  
Chromatographic Techniques ◽  
The Past ◽  
Real Matrices

Over the past 15 years, chromatographic techniques with mass spectrometric detection have been increasingly used to monitor the rapidly expanded list of regulated flavour and fragrance ingredients. This trend entails a need for good quantification practices suitable for complex media, especially for multi-analytes. In this article, we present experimental precautions needed to perform the analyses and ways to process the data according to the most recent approaches. This notably includes the identification of analytes during their quantification and method validation, when applied to real matrices, based on accuracy profiles. A brief survey of application studies based on such practices is given. This article is part of the themed issue ‘Quantitative mass spectrometry’.

Label-assisted laser desorption/ionization mass spectrometry (LA-LDI-MS): use of pyrene aldehyde for detection of biogenic amines, amino acids and peptides

RSC Advances ◽  
2015 ◽  
Vol 5 (129) ◽  
pp. 106912-106917 ◽  
Author(s):  
Arundhoti Mandal ◽  
Amit K. Das ◽  
Amit Basak
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Small Molecules ◽  
Biogenic Amines ◽  
Laser Desorption ◽  
Mass Spectrometric ◽  
Ionization Mass ◽  
Laser Desorption Ionization ◽  
Mass Spectrometric Measurement ◽  
Desorption Ionization

Label-assisted laser desorption/ionization (LA-LDI) technique has recently been applied to the detection of biologically important small molecules through a time of flight (TOF) mass spectrometric measurement.

scholarly journals Early neurotransmission impairment in non-invasive Alzheimer Disease detection

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Carmen Peña-Bautista ◽  
Isabel Torres-Cuevas ◽  
Miguel Baquero ◽  
Inés Ferrer ◽  
Lorena García ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Alzheimer Disease ◽  
Healthy Subjects ◽  
Tandem Mass ◽  
Cognitive Tests ◽  
Non Invasive ◽  
Amino Acids And Derivatives ◽  
Diagnosis Model ◽  
Potential Biomarkers

Abstract Alzheimer Disease (AD) is a pathology suffered by millions of people worldwide and it has a great social and economic impact. Previous studies reported a relationship between alterations in different amino acids and derivatives involved in neurotransmission systems and cognitive impairment. Therefore, in this study the neurotransmission impairment associated to early AD has been evaluated. For this purpose, different amino acids and derivatives were determined in saliva samples from AD patients and healthy subjects, by means of an analytical method based on chromatography coupled to tandem mass spectrometry. Results showed statistically significant differences in salivary levels for the compounds myo-inositol, creatine and acetylcholine; and other compounds (myo-inositol, glutamine, creatine, acetylcholine) showed significant correlations with some cognitive tests scores. Therefore, these compounds were included in a multivariate analysis and the corresponding diagnosis model showed promising indices (AUC 0.806, sensitivity 61%, specificity 92%). In conclusion, some amino acids and derivatives involved in neurotransmission impairment could be potential biomarkers in early and non-invasive AD detection.

scholarly journals Analysis of ion currents in mass spectrometric profiles using glioblastoma tissue

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 37
Author(s):  
Anatoly A. Sorokin ◽  
Evgeny S. Zhvansky ◽  
Denis S. Zavorotnyuk ◽  
Vsevolod A. Shurkhay ◽  
Denis S. Bormotov ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Biological Samples ◽  
Chromatographic Separation ◽  
Ambient Ionization ◽  
Mass Spectrometric ◽  
Ion Current ◽  
Molecular Profile ◽  
Ionization Mass ◽  
Sample Type ◽  
Ion Currents

Background: The development of direct ambient ionization methods makes way for fast mass-spectrometry profiling of biological samples, which has great potential in medicine. Those methods, unlike traditional mass spectrometric analysis with chromatographic separation, are not able to take into account inter-ion interaction, ion suppression, and matrix effect due to the absence of chromatographic separation of the mixture components. So dynamics of ion current during direct ambient ionization mass-spectra is governed by the component micro-extraction and electrospray ionization influenced by the geometry of the sample, its position, and internal heterogeneity. Despite the progress in mass-spectrometry of biological samples, not much is known about the influence of sample type and structure on its molecular profile peculiarities. Methods: In this work, we propose to use analysis of the correlation between individual ion currents for a better understanding of ion current variability sources and grouping ions of high biological importance. Several fragments of glioblastoma tissue from a single patient are used for these purposes. Results: Ion currents have different dynamics considering different ions in different fragments. The correlation of two selected ion currents could be positive or negative for single fragment measurement. Correlations have persistent or alternating signs in different fragments for two selected ions. The spread of correlations of each pair of ion currents is calculated for evaluation of the signs’ stability. Conclusions: We were able to group ions according to the primary reason for their variabilities such as micro-extraction, mass-spectrometry measurement, or specimens' properties. Such grouping would allow the development of more reliable and reproducible methods of mass-spectrometry data analysis and improve the accuracy of results of its application in medicine.

Sign in / Sign up

Export Citation Format

Share Document