Diffuse optical imaging

2009 ◽  
Vol 367 (1900) ◽  
pp. 3055-3072 ◽  
Author(s):  
Adam Gibson ◽  
Hamid Dehghani
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
State Of The Art ◽  
Imaging Technique ◽  
Multimodality Imaging ◽  
Natural Extension ◽  
Psychological Research ◽  
Functional Brain Imaging ◽  
Diffuse Optical Imaging ◽  
Image Reconstruction Techniques

Diffuse optical imaging is a medical imaging technique that is beginning to move from the laboratory to the hospital. It is a natural extension of near-infrared spectroscopy (NIRS), which is now used in certain niche applications clinically and particularly for physiological and psychological research. Optical imaging uses sophisticated image reconstruction techniques to generate images from multiple NIRS measurements. The two main clinical applications—functional brain imaging and imaging for breast cancer—are reviewed in some detail, followed by a discussion of other issues such as imaging small animals and multimodality imaging. We aim to review the state of the art of optical imaging.

In-vivo near-infrared optical imaging of growing osteosarcoma cell lesions xenografted in mice: dual-channel quantitative evaluation of volume and mineralization

2011 ◽  
Vol 52 (9) ◽  
pp. 978-988 ◽  
Author(s):  
Hitoshi Nakayama ◽  
Tomoyuki Kawase ◽  
Kazuhiro Okuda ◽  
Larry F Wolff ◽  
Hiromasa Yoshie
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Imaging Technique ◽  
Ex Vivo ◽  
Acquisition Time ◽  
Osteosarcoma Cell ◽  
Dual Channel ◽  
Nir Imaging ◽  
Ex Vivo Imaging

Background In a previous study using a rodent osteosarcoma-grafted rat model, in which cell-dependent mineralization was previously demonstrated to proportionally increase with growth, we performed a quantitative analysis of mineral deposit formation using 99mTc-HMDP and found some weaknesses, such as longer acquisition time and narrower dynamic ranges (i.e. images easily saturated). The recently developed near-infrared (NIR) optical imaging technique is expected to non-invasively evaluate changes in living small animals in a quantitative manner. Purpose To test the feasibility of NIR imaging with a dual-channel system as a better alternative for bone scintigraphy by quantitatively evaluating mineralization along with the growth of osteosarcoma lesions in a mouse-xenograft model. Material and Methods The gross volume and mineralization of osteosarcoma lesions were evaluated in living mice simultaneously with dual-channels by NIR dye-labeled probes, 2-deoxyglucose (DG) and pamidronate (OS), respectively. To verify these quantitative data, retrieved osteosarcoma lesions were then subjected to ex-vivo imaging, weighing under wet conditions, microfocus-computed tomography (μCT) analysis, and histopathological examination. Results Because of less scattering and no anatomical overlapping, as generally shown, specific fluorescence signals targeted to the osteosarcoma lesions could be determined clearly by ex-vivo imaging. These data were well positively correlated with the in-vivo imaging data ( r > 0.8, P < 0.02). Other good to excellent correlations ( r > 0.8, P < 0.02) were observed between DG accumulation and tumor gross volume and between OS accumulation and mineralization volume. Conclusion This in-vivo NIR imaging technique using DG and OS is sensitive to the level to simultaneously detect and quantitatively evaluate the growth and mineralization occuring in this type of osteosarcoma lesions of living mice without either invasion or sacrifice. By possible mutual complementation, this dual imaging system might be useful for accurate diagnosis even in the presence of overlapping tissues.

scholarly journals In Vivo Resolution of Multiexponential Decays of Multiple Near-Infrared Molecular Probes by Fluorescence Lifetime-Gated Whole-Body Time-Resolved Diffuse Optical Imaging

2007 ◽  
Vol 6 (4) ◽  
pp. 7290.2007.00020 ◽  
Author(s):  
Walter Akers ◽  
Frederic Lesage ◽  
Dewey Holten ◽  
Samuel Achilefu
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Near Infrared ◽  
Molecular Probes ◽  
Whole Body ◽  
Diffuse Optical Imaging ◽  
Fluorescence Lifetimes ◽  
Time Resolved

The biodistribution of two near-infrared fluorescent agents was assessed in vivo by time-resolved diffuse optical imaging. Bacteriochlorophyll a (BC) and cypate-glysine-arginine-aspartic acid-serine-proline-lysine-OH (Cyp-GRD) were administered separately or combined to mice with subcutaneous xenografts of human breast adenocarcinoma and slow-release estradiol pellets for improved tumor growth. The same excitation (780 nm) and emission (830 nm) wavelengths were used to image the distinct fluorescence lifetime distribution of the fluorescent molecular probes in the mouse cancer model. Fluorescence intensity and lifetime maps were reconstructed after raster-scanning whole-body regions of interest by time-correlated single-photon counting. Each captured temporal point-spread function (TPSF) was deconvolved using both a single and a multiexponental decay model to best determine the measured fluorescence lifetimes. The relative signal from each fluorophore was estimated for any region of interest included in the scanned area. Deconvolution of the individual TPSFs from whole-body fluorescence intensity scans provided corresponding lifetime images for comparing individual component biodistribution. In vivo fluorescence lifetimes were determined to be 0.8 ns (Cyp-GRD) and 2 ns (BC). This study demonstrates that the relative biodistribution of individual fluorophores with similar spectral characteristics can be compartmentalized by using the time-domain fluorescence lifetime gating method.

Multimodality imaging in valvular heart disease: how to use state-of-the-art technology in daily practice

2020 ◽  
Author(s):  
Anna Reid ◽  
Philipp Blanke ◽  
Jeroen J Bax ◽  
Jonathon Leipsic
Keyword(s):  
Heart Disease ◽  
Valvular Heart Disease ◽  
State Of The Art ◽  
Imaging Technique ◽  
Multimodality Imaging ◽  
Management Strategies ◽  
Daily Practice ◽  
Current Evidence ◽  
Technological Advancement ◽  
Accurate Quantification

Abstract Our understanding of the complexities of valvular heart disease (VHD) has evolved in recent years, primarily because of the increased use of multimodality imaging (MMI). Whilst echocardiography remains the primary imaging technique, the contemporary evaluation of patients with VHD requires comprehensive analysis of the mechanism of valvular dysfunction, accurate quantification of severity, and active exclusion extravalvular consequences. Furthermore, advances in surgical and percutaneous therapies have driven the need for meticulous multimodality imaging to aid in patient and procedural selection. Fundamental decision-making regarding whom, when, and how to treat patients with VHD has become more complex. There has been rapid technological advancement in MMI; many techniques are now available in routine clinical practice, and their integration into has the potential to truly individualize management strategies. This review provides an overview of the current evidence for the use of MMI in VHD, and how various techniques within each modality can be used practically to answer clinical conundrums.

scholarly journals Determinants of skeletal muscle oxygen consumption assessed by near-infrared diffuse correlation spectroscopy during incremental handgrip exercise

2019 ◽  
Vol 127 (3) ◽  
pp. 698-706 ◽  
Author(s):  
Ryan Rosenberry ◽  
Wesley J. Tucker ◽  
Mark J. Haykowsky ◽  
Darian Trojacek ◽  
Houda H. Chamseddine ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Blood Flow ◽  
Optical Imaging ◽  
Brachial Artery ◽  
Near Infrared ◽  
Imaging Technique ◽  
Correlation Spectroscopy ◽  
Handgrip Exercise ◽  
Diffuse Correlation Spectroscopy ◽  
Optical Imaging Technique

Near-infrared diffuse correlation spectroscopy (DCS) is a rapidly evolving optical imaging technique for the assessment of skeletal muscle O2 utilization (mVO2). We compared DCS-derived determinants of mVO2 with conventional measures [blood flow by brachial artery Doppler ultrasound and venous O2 saturation ([Formula: see text])] in eight volunteers at rest and during incremental handgrip exercise. Brachial artery blood flow and DCS-derived blood flow index (BFI) were linearly related (R2 = 0.57) and increased with each workload, whereas [Formula: see text] decreased from 65.3 ± 2.5% (rest) to 39.9 ± 3.0% (light exercise; P < 0.01) with no change thereafter. In contrast, DCS-derived tissue O2 saturation decreased progressively with each incremental stage ( P < 0.01), driven almost entirely by an initial steep rise in deoxyhemoglobin/myoglobin, followed by a linear increase thereafter. Whereas seemingly disparate at first glance, we believe these two approaches provide similar information. Indeed, by plotting the mean convective O2 delivery and diffusive O2 conductance, we show that the initial increase in mVO2 during the transition from rest to exercise was achieved by a greater increase in diffusive O2 conductance versus convective O2 delivery (10-fold vs. 4-fold increase, respectively), explaining the initial decline in [Formula: see text]. In contrast, the increase in mVO2 from light to heavy exercise was achieved by equal increases (1.8-fold) in convective O2 delivery and diffusive O2 conductance, explaining the plateau in [Formula: see text]. That DCS-derived BFI and deoxyhemoglobin/myoglobin (surrogate measure of O2 extraction) share the same general biphasic pattern suggests that both DCS and conventional approaches provide complementary information regarding the determinants of mVO2. NEW & NOTEWORTHY Near-infrared diffuse correlation spectroscopy (DCS) is an emerging optical imaging technique for quantifying skeletal muscle O2 delivery and utilization at the microvascular level. Here, we show that DCS provides complementary insight into the determinants of muscle O2 consumption across a wide range of exercise intensities, further establishing the utility of DCS.

scholarly journals Diffuse Optical Imaging: Safe and Functional Medical Imaging Technique

2018 ◽  
Vol 1 (2) ◽  
pp. 1-4
Author(s):  
Omnia Hamdy ◽  
Mahmoud F. Hassan ◽  
Nahed H. Solouma ◽  
Nahed H. Solouma
Keyword(s):  
Medical Imaging ◽  
Optical Imaging ◽  
Imaging Technique ◽  
Medical Applications ◽  
Imaging Method ◽  
Diffuse Optical Imaging ◽  
Optical Imaging Technique

Optical imaging method provides safe and encouraging tool in many medical applications. In this editorial, principle operation, instrumentation, medical applications and advantages of diffuse optical imaging technique are presented and discussed.

Sign in / Sign up

Export Citation Format

Share Document