scholarly journals Multifunctional scanning ion conductance microscopy

2017 ◽  
Vol 473 (2200) ◽  
pp. 20160889 ◽  
Author(s):  
Ashley Page ◽  
David Perry ◽  
Patrick R. Unwin
Keyword(s):  
Surface Charge ◽  
Single Cells ◽  
Three Dimensional ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
New Methods ◽  
Single Probe ◽  
Advanced Control ◽  
Opening Up ◽  
Electrochemical Microscopy

Scanning ion conductance microscopy (SICM) is a nanopipette-based technique that has traditionally been used to image topography or to deliver species to an interface, particularly in a biological setting. This article highlights the recent blossoming of SICM into a technique with a much greater diversity of applications and capability that can be used either standalone, with advanced control (potential–time) functions, or in tandem with other methods. SICM can be used to elucidate functional information about interfaces, such as surface charge density or electrochemical activity (ion fluxes). Using a multi-barrel probe format, SICM-related techniques can be employed to deposit nanoscale three-dimensional structures and further functionality is realized when SICM is combined with scanning electrochemical microscopy (SECM), with simultaneous measurements from a single probe opening up considerable prospects for multifunctional imaging. SICM studies are greatly enhanced by finite-element method modelling for quantitative treatment of issues such as resolution, surface charge and (tip) geometry effects. SICM is particularly applicable to the study of living systems, notably single cells, although applications extend to materials characterization and to new methods of printing and nanofabrication. A more thorough understanding of the electrochemical principles and properties of SICM provides a foundation for significant applications of SICM in electrochemistry and interfacial science.

scholarly journals Mapping Surface Charge of Individual Microdomains with Scanning Ion Conductance Microscopy

2018 ◽  
Vol 5 (20) ◽  
pp. 2986-2990 ◽  
Author(s):  
Cheng Zhu ◽  
Lushan Zhou ◽  
Myunghoon Choi ◽  
Lane A. Baker
Keyword(s):  
Surface Charge ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

Gauging surface charge distribution of live cell membrane by ionic current change using scanning ion conductance microscopy

Nanoscale ◽  
2021 ◽  
Author(s):  
Feng Chen ◽  
Jin He ◽  
Prakash Manandhar ◽  
Yizi Yang ◽  
Peidang Liu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Surface Charge ◽  
Charge Distribution ◽  
Ionic Current ◽  
Live Cells ◽  
Ion Conductance ◽  
Physiological Conditions ◽  
Scanning Ion Conductance Microscopy ◽  
Surface Charge Distribution ◽  
Current Change

The distribution of surface charge and potential of cell membrane plays an indispensable role in cellular activities. However, probing surface charge of live cells in physiological conditions, until recently, remains...

scholarly journals Fast Nanoscale Surface Charge Mapping with Pulsed-Potential Scanning Ion Conductance Microscopy

2016 ◽  
Vol 88 (22) ◽  
pp. 10854-10859 ◽  
Author(s):  
Ashley Page ◽  
David Perry ◽  
Philip Young ◽  
Daniel Mitchell ◽  
Bruno G. Frenguelli ◽  
...  
Keyword(s):  
Surface Charge ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy

scholarly journals Scanning ion conductance microscopy reveals differences in the ionic environments of gram positive and negative bacteria

2020 ◽  
Author(s):  
Kelsey Cremin ◽  
Bryn Jones ◽  
James Teahan ◽  
Gabriel N. Meloni ◽  
David Perry ◽  
...  
Keyword(s):  
Cell Wall ◽  
Surface Charge ◽  
Wall Structure ◽  
Current Response ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Fem Model ◽  
Ion Conductance ◽  
E Coli ◽  
Scanning Ion Conductance Microscopy

AbstractThis paper reports on the use of scanning ion conductance microscopy (SICM) to locally map the ionic properties and charge environment of two live bacterial strains: the gramnegative Escherichia coli and the gram-positive Bacillus subtilis. SICM results find heterogeneities across the bacterial surface, and significant differences among the grampositive and -negative bacteria. The bioelectrical environment of the B. subtilis was found to be considerably more negatively charged compared to E. coli. SICM measurements, fitted to a simplified finite element method (FEM) model, revealed surface charge values of −80 to −140 mC m−2 for the gram-negative E. coli. The gram-positive B. subtilis show a much higher conductivity around the cell wall, and surface charge values between −350 and −450 mC m−2 were found using the same simplified model. SICM was also able to detect regions of high negative charge near B. subtilis, not detected in the topographical SICM response and attributed to extracellular polymeric substance. To further explore how the B. subtilis cell wall structure can influence the SICM current response, a more comprehensive FEM model, accounting for the physical properties of the gram-positive cell wall, was developed. The new model provides a more realistic description of the cell wall and allowed investigation of the relation between its key properties and SICM currents, building foundations to further investigate and improve understanding of the gram-positive cellular microenvironment.Abstract Figure

scholarly journals Time-Resolved Scanning Ion Conductance Microscopy for Three-Dimensional Tracking of Nanoscale Cell Surface Dynamics

ACS Nano ◽  
2021 ◽  
Author(s):  
Samuel M. Leitao ◽  
Barney Drake ◽  
Katarina Pinjusic ◽  
Xavier Pierrat ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
Cell Surface ◽  
Three Dimensional ◽  
Surface Dynamics ◽  
Ion Conductance ◽  
Time Resolved ◽  
Scanning Ion Conductance Microscopy

scholarly journals A cost-efficient approach for simultaneous scanning electrochemical microscopy and scanning ion conductance microscopy

2020 ◽  
Vol 151 (8) ◽  
pp. 1249-1255
Author(s):  
Stefan Wert ◽  
Simona Baluchová ◽  
Karolina Schwarzová-Pecková ◽  
Silvia Sedláková ◽  
Andrew Taylor ◽  
...  
Keyword(s):  
Scanning Electrochemical Microscopy ◽  
Ion Conductance ◽  
Efficient Approach ◽  
Scanning Ion Conductance Microscopy ◽  
Cost Efficient ◽  
Electrochemical Microscopy

scholarly journals Time-resolved scanning ion conductance microscopy for three-dimensional tracking of nanoscale cell surface dynamics

2021 ◽  
Author(s):  
Samuel M. Leitao ◽  
Barney Drake ◽  
Katarina Pinjusic ◽  
Xavier Pierrat ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
High Speed ◽  
Three Dimensional ◽  
Cellular Structures ◽  
Surface Dynamics ◽  
Dynamic Changes ◽  
Ion Conductance ◽  
Time Resolved ◽  
Scanning Ion Conductance Microscopy ◽  
3D Information

Understanding cellular function requires high-resolution information about cellular structures as well as their evolution over time. The major challenge is to obtain three-dimensional (3D) information at nanometer resolution without affecting the viability of the cells and avoiding interference with the process. Here, we develop a scanning ion conductance microscope (SICM) for high-speed and long term imaging that can resolve spatiotemporally diverse processes on the cell membrane. We tracked dynamic changes in live cell morphology with nanometer details and temporal ranges of sub-second to days, imagining diverse processes ranging from endocytosis, micropinocytosis, and mitosis, to bacterial infection and cell differentiation in cancer cells. This technique enables a detailed look at membrane events and may offer insights into cell-cell interactions for infection, immunology, and cancer research.

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