Gauging surface charge distribution of live cell membrane by ionic current change using scanning ion conductance microscopy

Nanoscale ◽  
2021 ◽  
Author(s):  
Feng Chen ◽  
Jin He ◽  
Prakash Manandhar ◽  
Yizi Yang ◽  
Peidang Liu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Surface Charge ◽  
Charge Distribution ◽  
Ionic Current ◽  
Live Cells ◽  
Ion Conductance ◽  
Physiological Conditions ◽  
Scanning Ion Conductance Microscopy ◽  
Surface Charge Distribution ◽  
Current Change

The distribution of surface charge and potential of cell membrane plays an indispensable role in cellular activities. However, probing surface charge of live cells in physiological conditions, until recently, remains...

scholarly journals Direct measurement of surface charge distribution in phase separating supported lipid bilayers

Nanoscale ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 4538-4544 ◽  
Author(s):  
Thomas Fuhs ◽  
Lasse Hyldgaard Klausen ◽  
Steffan Møller Sønderskov ◽  
Xiaojun Han ◽  
Mingdong Dong
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Lipid Bilayers ◽  
Surface Charge Density ◽  
Supported Lipid Bilayers ◽  
Local Surface ◽  
Surface Charge Distribution

The local surface charge density of the cell membrane influences regulation and localization of membrane proteins.

scholarly journals Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Surface Charge Density ◽  
Single Cells ◽  
Fluorescent Light ◽  
Fluorescent Nanoparticles ◽  
Cell Surface Charge ◽  
Surface Charge Distribution

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

scholarly journals Nanoscale Cell Membrane Fluctuations Measured by Scanning Ion Conductance Microscopy

2013 ◽  
Vol 104 (2) ◽  
pp. 317a
Author(s):  
Yusuke Mizutani ◽  
Zen Ishikura ◽  
Myung-Hoon Choi ◽  
Sang-Joon Cho ◽  
Takaharu Okajima
Keyword(s):  
Cell Membrane ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Membrane Fluctuations

Scanning Ion Conductance Microscopy for High-Resolution Topography of Soft Samples Including Live Cells

2011 ◽  
Vol 17 (S2) ◽  
pp. 236-237
Author(s):  
G De Filippi ◽  
C Moore
Keyword(s):  
High Resolution ◽  
Live Cells ◽  
Ion Conductance ◽  
Scanning Ion Conductance Microscopy ◽  
Soft Samples

Extended abstract of a paper presented at Microscopy and Microanalysis 2011 in Nashville, Tennessee, USA, August 7–August 11, 2011.

EQCM as a unique tool for determination of ionic fluxes in microporous carbons as a function of surface charge distribution

2010 ◽  
Vol 12 (12) ◽  
pp. 1718-1721 ◽  
Author(s):  
Sergey Sigalov ◽  
Mikhael D. Levi ◽  
Gregory Salitra ◽  
Doron Aurbach ◽  
Joachim Maier
Keyword(s):  
Surface Charge ◽  
Charge Distribution ◽  
Microporous Carbons ◽  
Ionic Fluxes ◽  
Surface Charge Distribution
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