scholarly journals Forensic efficiency estimate and phylogenetic analysis for Chinese Kyrgyz ethnic group revealed by a panel of 21 short tandem repeats

2018 ◽  
Vol 5 (6) ◽  
pp. 172089 ◽  
Author(s):  
Yuxin Guo ◽  
Chong Chen ◽  
Tong Xie ◽  
Wei Cui ◽  
Haotian Meng ◽  
...  
Keyword(s):  
Ethnic Group ◽  
Phylogenetic Trees ◽  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Forensic Genetics ◽  
Genetic Relationships ◽  
Individual Identification ◽  
Forensic Sciences ◽  
Dna Index ◽  
Short Tandem

Short tandem repeats (STRs) with a high level of polymorphisms and convenient detection method play an indispensable role in human population and forensic genetics. Recently, we detected the 21 autosomal non-combined DNA index system (non-CODIS) STR loci in a Kyrgyz ethnic group, calculated their forensic parameters and analysed its genetic relationships with reference populations from China. In total, 168 alleles were observed at 21 non-CODIS STRs with corresponding allelic frequencies from 0.0016 to 0.4788. No significant deviations at these STRs were observed from the Hardy–Weinberg equilibrium. The values of cumulative power of discrimination and probability of exclusion for all the 21 non-CODIS STRs were 0.99999999999999999998835 and 0.9999994002, respectively. Furthermore, the analyses of phylogenetic trees, genetic distances and interpopulation differentiations demonstrated that the Kyrgyz group had relatively close genetic relationships with the Uygur and Kazak groups. These 21 non-CODIS STRs were characterized by high genetic diversities in the Kyrgyz group and could be applied as a robust tool for individual identification and kinship testing in forensic sciences.

Characterization of human short tandem repeats (STRs) for individual identification using the Ion Torrent

2015 ◽  
Vol 9 (2) ◽  
pp. 164-172 ◽  
Author(s):  
Seri Lim ◽  
Jong Pil Youn ◽  
Sang Ok Moon ◽  
Youn Hyung Nam ◽  
Seung Bum Hong ◽  
...  
Keyword(s):  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Individual Identification ◽  
Ion Torrent ◽  
Short Tandem

scholarly journals Forensic Genetics and Genotyping

2019 ◽  
Vol 20 (2) ◽  
pp. 75-86
Author(s):  
Katarina Vitoševic ◽  
Danijela Todorovic ◽  
Zivana Slovic ◽  
Radica Zivkovic-Zaric ◽  
Milos Todorovic
Keyword(s):  
Genetic Markers ◽  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Forensic Genetics ◽  
Personal Identification ◽  
Hair Color ◽  
Forensic Dna ◽  
Kinship Analysis ◽  
Dna Profile ◽  
Short Tandem

Abstract Forensic genetics represents a combination of molecular and population genetics. Personal identification and kinship analysis (e.g. paternity testing) are the two main subjects of forensic DNA analysis. Biological specimens from which DNA is isolated are blood, semen, saliva, tissues, bones, teeth, hairs. Genotyping has become a basis in the characterization of forensic biological evidence. It is performed using a variety of genetic markers, which are divided into two large groups: bi-allelic (single-nucleotide polymorphisms, SNP) and multi-allelic polymorphisms (variable number of tandem repeats, VNTR and short tandem repeats, STR). This review describes the purpose of genetic markers in forensic investigation and their limitations. The STR loci are currently the most informative genetic markers for identity testing, but in cases without a suspect SNP can predict offender’s ancestry and phenotype traits such as skin, eyes and hair color. Nowadays, many countries worldwide have established forensic DNA databases based on autosomal short tandem repeats and other markers. In order for DNA profile database to be useful at a national or international level, it is essential to standardize genetic markers used in laboratories.

scholarly journals Comprehensive Insights Into Forensic Features and Genetic Background of Chinese Northwest Hui Group Using Six Distinct Categories of 231 Molecular Markers

2021 ◽  
Vol 12 ◽  
Author(s):  
Chong Chen ◽  
Xiaoye Jin ◽  
Xingru Zhang ◽  
Wenqing Zhang ◽  
Yuxin Guo ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Single Nucleotide Polymorphisms ◽  
Genetic Background ◽  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Genetic Relationships ◽  
Population Data ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Short Tandem

The Hui minority is predominantly composed of Chinese-speaking Islamic adherents distributed throughout China, of which the individuals are mainly concentrated in Northwest China. In the present study, we employed the length and sequence polymorphisms-based typing system of 231 molecular markers, i.e., amelogenin, 22 phenotypic-informative single nucleotide polymorphisms (PISNPs), 94 identity-informative single nucleotide polymorphisms (IISNPs), 24 Y-chromosomal short tandem repeats (Y-STRs), 56 ancestry-informative single nucleotide polymorphisms (AISNPs), 7 X-chromosomal short tandem repeats (X-STRs), and 27 autosomal short tandem repeats (A-STRs), into 90 unrelated male individuals from the Chinese Northwest Hui group to comprehensively explore its forensic characteristics and genetic background. Total of 451 length-based and 652 sequence-based distinct alleles were identified from 58 short tandem repeats (STRs) in 90 unrelated Northwest Hui individuals, denoting that the sequence-based genetic markers could pronouncedly provide more genetic information than length-based markers. The forensic characteristics and efficiencies of STRs and IISNPs were estimated, both of which externalized high polymorphisms in the Northwest Hui group and could be further utilized in forensic investigations. No significant departure from the Hardy–Weinberg equilibrium (HWE) expectation was observed after the Bonferroni correction. Additionally, four group sets of reference population data were exploited to dissect the genetic background of the Northwest Hui group separately from different perspectives, which contained 26 populations for 93 IISNPs, 58 populations for 17 Y-STRs, 26 populations for 55 AISNPs (raw data), and 109 populations for 55 AISNPs (allele frequencies). As a result, the analyses based on the Y-STRs indicated that the Northwest Hui group primarily exhibited intimate genetic relationships with reference Hui groups from Chinese different regions except for the Sichuan Hui group and secondarily displayed close genetic relationships with populations from Central and West Asia, as well as several Chinese groups. However, the AISNP analyses demonstrated that the Northwest Hui group shared more intimate relationships with current East Asian populations apart from reference Hui group, harboring the large proportion of ancestral component contributed by East Asia.

scholarly journals Animal Forensic Genetics

Genes ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 515
Author(s):  
Adrian Linacre
Keyword(s):  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Potential Role ◽  
Forensic Genetics ◽  
Human Species ◽  
Domestic Species ◽  
Traditional Medicines ◽  
Increased Sensitivity ◽  
Forensic Testing ◽  
Short Tandem

Animal forensic genetics, where the focus is on non-human species, is broadly divided in two: domestic species and wildlife. When traces of a domestic species are relevant to a forensic investigation the question of species identification is less important, as the material comes from either a dog or a cat for instance, but more relevant may be the identification of the actual pet. Identification of a specific animal draws on similar methods to those used in human identification by using microsatellite markers. The use of cat short tandem repeats to link a cat hair to a particular cat paved the way for similar identification of dogs. Wildlife forensic science is becoming accepted as a recognised discipline. There is growing acceptance that the illegal trade in wildlife is having devasting effects on the numbers of iconic species. Loci on the mitochondrial genome are used to identify the most likely species present. Sequencing the whole locus may not be needed if specific bases can be targeted. There can be benefits of increased sensitivity using mitochondrial loci for species testing, but occasionally there is an issue if hybrids are present. The use of massively parallel DNA sequencing has a role in the identification of the ingredients of traditional medicines where studies found protected species to be present, and a potential role in future species assignments. Non-human animal forensic testing can play a key role in investigations provided that it is performed to the same standards as all other DNA profiling processes.

scholarly journals Karakteristik Genetik Populasi Kuno Pulau Bali: Sanur dan Gilimanuk

2018 ◽  
Vol 17 (1) ◽  
pp. 39
Author(s):  
Rusyad Adi Suryanto ◽  
Toetik Koesbardiati ◽  
Delita Bayu Murti ◽  
Ahmad Yudianto ◽  
Anak Agung Putu Santiasa Putra
Keyword(s):  
Index System ◽  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Human Genetics ◽  
Human Migration ◽  
Tropical Disease ◽  
Biological Anthropology ◽  
Neolithic Period ◽  
Dna Index ◽  
Short Tandem

AbstractThe study of ancient human migration and peopling in Indonesia still raises debate until now, both from the perspective of biological anthropology, human genetics or archaeological. The debate was always open space again to do some research about that. We concentrated with samples of ancient Bali, the findings of human remains from Gilimanuk (Melaya, Jembrana) and Semawang (Sanur, Denpasar). Relatively, Bali is an island located in the centre of Indonesian Archipelago, which may represent a major pathway of human migration and distribution according to the outer arc islands. The research aimed to describe human genetic variation of the two archeological sites of ancient Bali. Based locus short tandem repeats (STR) combined DNA index system (CODIS), which CSF1PO, TH01 and TP0X, the research took a sample of six individual human ancient Bali, which includes each of the three individual from Semawang and Gilimanuk site. The process of genetic research has been done at the Institute of Tropical Disease Laboratory of Human Genetics, Airlangga University. Semawang and Gilimanuk derived from different populations based on the analysis of its CTT loci visualization. The results with reference to all possible aspects of archaeology and biological anthropology further enrich the wealth of knowledge about human migration events in Indonesia around the Neolithic period, the early times of increasingly massive mongoloid migrations to the Archipelago region. The results also further strengthen the results of previous genetic studies of Bali population. Balinese has undergone a genetic mixture of various immigrant populations since the Neolithic period.AbstrakPenelitian migrasi dan penghunian manusia kuno di Indonesia masih memunculkan perdebatan sampai kini, baik dari perspektif antropologi biologis, genetika manusia atau arkeologis. Perdebatan itu selalu membuka ruang lagi untuk melakukan penelitian perihal itu. Kali ini kami berkonsentrasi dengan sampel Bali Kuno, yakni temuan sisa-sisa manusia dari Gilimanuk (Melaya, Jembrana) dan Semawang (Sanur, Denpasar). Bali merupakan pulau yang relatif terletak di tengah gugusan kepulauan Indonesia, di mana dapat mewakili jalur besar migrasi dan persebaran manusia seturut rute pulau-pulau busur luarnya. Penelitian ini bertujuan untuk mendeskripsikan variasi genetik manusia kuno dari dua situs arkeologis Bali itu. Berdasarkan lokus short tandem repeats (STR) combined DNA index system (CODIS), yakni CSF1PO, TH01 dan TP0X, penelitian ini mengambil sampel enam individu manusia Bali Kuno, yang meliputi masing-masing tiga individu Semawang dan Gilimanuk. Proses penelitian genetik itu telah dikerjakan di Laboratory of Human Genetics, Institute of Tropical Disease, Universitas Airlangga. Sampel Semawang dan Gilimanuk berasal dari populasi yang berbeda berdasarkan analisis visualisasi lokus CTT-nya. Hasil penelitian ini dengan merujuk semua kemungkinan aspek arkeologis dan antropologi biologisnya makin memperkaya khazanah pengetahuan tentang peristiwa migrasi manusia di Indonesia sekitar masa Neolitik, yang menjadi masa awal makin masifnya migrasi Mongoloid ke kawasan Nusantara. Hasil penelitian ini juga makin menguatkan hasil-hasil penelitian genetika populasi Bali sebelumnya bahwa populasi Bali dari sejak Neolitik sampai sekitar masa yang lebih resen diturunkan oleh banyak leluhur atau banyak sumber gen. Penduduk Bali telah mengalami percampuran genetik dari berbagai populasi pendatang sejak Neolitik atau awal Tarikh Masehi.

D18S535, D1S1656 and D10S2325: three efficient short tandem repeats for forensic genetics

1999 ◽  
Vol 112 (6) ◽  
pp. 360-363 ◽  
Author(s):  
P. Wiegand ◽  
M. V. Lareu ◽  
M. Schürenkamp ◽  
M. Kleiber ◽  
B. Brinkmann
Keyword(s):  
Short Tandem Repeats ◽  
Tandem Repeats ◽  
Forensic Genetics ◽  
Short Tandem

scholarly journals The Polymorphism Analyses of Short Tandem Repeats as a Basis for Understanding the Genetic Characteristics of the Guanzhong Han Population

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Shuyan Mei ◽  
Yanfang Liu ◽  
Congying Zhao ◽  
Hui Xu ◽  
Shuanglin Li ◽  
...  
Keyword(s):  
Tandem Repeats ◽  
Principal Component ◽  
Individual Identification ◽  
Scaling Analysis ◽  
Dna Index ◽  
Genetic Characteristics ◽  
Han Population ◽  
Allelic Frequencies ◽  
Str Loci ◽  
Short Tandem

The short tandem repeat (STR) loci are polymorphic markers in the combined DNA index system (CODIS) and non-CODIS STR loci. Due to the highly polymorphic characteristic of STR loci, they are popular and widely used in forensic DNA typing laboratories. In this study, 22 STR loci (1 CODIS, 21 non-CODIS STR loci) and an Amelogenin locus were genotyped and analyzed in 590 unrelated individuals of the Guanzhong Han population. None of the 22 STR loci deviated from the Hardy–Weinberg equilibrium, and all the loci were in the linkage equilibrium state. We observed 247 alleles, and the corresponding allelic frequencies ranged from 0.0008 to 0.3695 in the Guanzhong Han population. The combined power of discrimination and the cumulative exclusion probability was 0.999 999 999 999 999 999 999 999 999 346 36 and 0.999 999 999 709 74, respectively. The results including Nei’s D A genetic distance, multidimensional scaling analysis, and principal component analysis showed that the Guanzhong Han population has closer genetic affinities with Northern Han, Chengdu Han, and Xinjiang Hui groups from China based on allelic frequencies of 15 overlapped STR loci from Guanzhong Han and 13 reference groups. The present results indicated that Microreader™ 23sp ID kit included highly polymorphic loci, and it could be well used for individual identification, paternity testing, and population genetics in the Guanzhong Han population.

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