scholarly journals Studying the association complex formation of atomoxetine and fluvoxamine with eosin Y and its application in their fluorimetric determination

2018 ◽  
Vol 5 (3) ◽  
pp. 170943 ◽  
Author(s):  
Sayed M. Derayea ◽  
Mahmoud A. Omar ◽  
Ahmed A. Abu-hassan
Keyword(s):  
Complex Formation ◽  
Pharmaceutical Formulations ◽  
Eosin Y ◽  
Fluorimetric Determination ◽  
Pharmaceutical Dosage Forms ◽  
Average Percentage ◽  
Binary Complexes ◽  
Spectrofluorimetric Method ◽  
Optimum Reaction

A simple, sensitive and non-extractive spectrofluorimetric method has been developed and validated for the determination of two psychoanaleptic drugs, atomoxetine and fluvoxamine, in pure forms and pharmaceutical dosage forms. The proposed method is based on the formation of binary complexes between eosin Y and the studied drugs in the presence of a Teorell–Stenhagen buffer. The quenching of the native fluorescence of eosin Y due to complex formation with the studied drugs was measured spectrofluorimetrically at 545 nm after excitation at 302 nm. At the optimum reaction conditions, the fluorescence quenching values (Δ F ) and concentrations were rectilinear over the concentration ranges of 0.2–2.2 and 0.3–2.2 µg ml −1 for atomoxetine and fluvoxamine, respectively. The developed method was successfully applied for the determination of the studied drugs in their pharmaceutical formulations with average percentage recoveries of 100.13 ± 0.66 and 99.69 ± 0.44 for atomoxetine and fluvoxamine, respectively ( n  = 5), without interference from common excipients.

scholarly journals Development and validation of a new spectrofluorimetric method for the determination of some beta-blockers through fluorescence quenching of eosin Y. Application to content uniformity test

2016 ◽  
Vol 14 (1) ◽  
pp. 258-266 ◽  
Author(s):  
Sayed M Derayea ◽  
Mahmoud A Omar ◽  
Mohamed Aboel-Kasem Abdel-Lateef ◽  
Ahmed I. Hassan
Keyword(s):  
Fluorescence Quenching ◽  
Beta Blockers ◽  
Dosage Forms ◽  
Ion Pair ◽  
Content Uniformity ◽  
Eosin Y ◽  
Pharmaceutical Dosage Forms ◽  
Quenching Effect ◽  
Spectrofluorimetric Method

AbstractA simple, rapid, sensitive and economic spectrofluorimetric method has been developed and validated for determination of some β-adrenergic blocking agents namely; betaxolol hydrochloride (BTX), carvedilol (CAR), labetalol hydrochloride (LBT), nebivolol hydrochloride (NEB) and propranolol hydrochloride (PRO). The method is based on the quenching effect of the cited drugs on the fluorescence intensity of eosin Y at pH 3.4 (acetate buffer). The fluorescence quenching is due to the formation of an ion-pair complex and was measured without extraction at 545 nm (λex. 301.5 nm). The factors affecting the formation of the ion-pair complex were carefully studied and optimized. Under the optimal conditions, the linear ranges for the relationship between the fluorescence quenching value and the concentration of the investigated drugs were 100-2500, 150-2500 and 50-2250 ng mL-1 for (BTX, CAR), (LBT, NEB) and (PRO) respectively. The method was validated according to ICH guidelines and was applied for determination of the cited drugs in pharmaceutical dosage forms with excellent recoveries. In addition, content uniformity testing of some commercial dosage forms was checked by the proposed method.

Application of a quinone-based fluorophore for spectrofluorimetric determination of albendazole in pure form and pharmaceutical formulations

2016 ◽  
Vol 8 (25) ◽  
pp. 5136-5141 ◽  
Author(s):  
Khalid A. M. Attia ◽  
Ahmad A. Mohamad ◽  
Mohamed S. Emara
Keyword(s):  
Charge Transfer ◽  
Complex Formation ◽  
Pharmaceutical Formulations ◽  
Pure Form ◽  
Spectrofluorimetric Method ◽  
Spectrofluorimetric Determination ◽  
Ct Complex

A spectrofluorimetric method was developed for the determination of albendazole (ALB) through charge transfer (CT) complex formation with 7,7,8,8-tetracyanoquinodimethane (TCNQ).

scholarly journals SPECTROFLUORIMETRIC DETERMINATION OF EOSIN CHEMILUMINESCENCE SYSTEM FOR THE ANALYSIS OF DABIGATRAN IN PHARMACEUTICAL FORMULATIONS

2019 ◽  
pp. 104-108
Author(s):  
DHANAPAL Y ◽  
SRUDHIVINOD V ◽  
MOHAMAD WASEEM A
Keyword(s):  
Method Development ◽  
Pharmaceutical Formulations ◽  
Calibration Graph ◽  
Eosin Y ◽  
Linear Calibration ◽  
Reaction Conditions ◽  
Quenching Effect ◽  
Spectrofluorimetric Determination ◽  
Optimum Reaction

Objectives: The objectives of this study were to develop a rapid, simple, and economical spectrofluorometric method for quantification of dabigatran from marketed formulation and its principle involved based on the protonated process of a binary mixture complex formation with eosin Y. Methods: A simple and precise spectrofluorometric technique was applied for the method development. It depends on measuring the quenching effect of the drug on the native fluorescence of eosin at excitation under the optimum reaction conditions. Results: The reaction linear calibration graph constructed between the fluorescence quenching valves flouresence intensity (ΔF) and the concentration ranges of 5–50 μg/ml. Spectrofluorometric analytical performance was validated by accuracy, precision, and specificity, and the results were satisfactory. Conclusion: This method was to develop a fast, simple, and economically applied successfully for the assay, and qualification of dabigatran tablet contains drug, also with different coformulated pharmaceutical formulations.

scholarly journals Rapid, Simple, and Sensitive Spectrofluorimetric Method for the Estimation of Ganciclovir in Bulk and Pharmaceutical Formulations

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Garima Balwani ◽  
Emil Joseph ◽  
Satish Reddi ◽  
Vibhu Nagpal ◽  
Ranendra N. Saha
Keyword(s):  
Standard Deviation ◽  
Limit Of Detection ◽  
Pharmaceutical Formulations ◽  
Calibration Graph ◽  
Limit Of Quantification ◽  
Average Percentage ◽  
Ich Guidelines ◽  
Spectrofluorimetric Method ◽  
Relative Standard

A new, simple, rapid, sensitive, accurate, and affordable spectrofluorimetric method was developed and validated for the estimation of ganciclovir in bulk as well as in marketed formulations. The method was based on measuring the native fluorescence of ganciclovir in 0.2 M hydrochloric acid buffer of pH 1.2 at 374 nm after excitation at 257 nm. The calibration graph was found to be rectilinear in the concentration range of 0.25–2.00 μg mL−1. The limit of quantification and limit of detection were found to be 0.029 μg mL−1and 0.010μg mL−1, respectively. The method was fully validated for various parameters according to ICH guidelines. The results demonstrated that the procedure is accurate, precise, and reproducible (relative standard deviation <2%) and can be successfully applied for the determination of ganciclovir in its commercial capsules with average percentage recovery of 101.31 ± 0.90.

scholarly journals Development of new spectrophotometric method for estimation of tenofovir disoproxil fumarate using MBTH reagent

2015 ◽  
Vol 4 (4) ◽  
pp. 378-381 ◽  
Author(s):  
Mannem Sri Varsha ◽  
N. Raghavendra Babu ◽  
Yenumula Padmavathi ◽  
P. Ravi Kumar
Keyword(s):  
Tenofovir Disoproxil Fumarate ◽  
Visible Region ◽  
Pharmaceutical Formulations ◽  
Pharmaceutical Journal ◽  
Specific Procedure ◽  
Analytical Validation ◽  
Pharmaceutical Dosage Forms ◽  
Secondary Amine Group ◽  
Validation Procedure

A new simple, sensitive and specific procedure has been developed for determination of tenofovir disoproxil fumarate in bulk and pharmaceutical dosage forms using MBTH reagent. The purpose of this analytical validation procedure is to validate it by laboratory experiments to prove that the method meets the minimum standards for laboratory use. 3-methyl-2-bezothiazoline hydrazone reacts with the secondary amine group of tenofovir in the presence of oxidizing agent, ferric chloride. The resulting apple green coloured chromogen when measured spectrophotometrically in visible region (i.e., 400-800nm) shows a maximum absorbance at 626.5nm. This method can be successfully applied for the determination of drug content in pharmaceutical formulations. The results of analysis have been validated statistically.DOI: http://dx.doi.org/10.3329/icpj.v4i4.22620 International Current Pharmaceutical Journal, March 2015, 4(4): 378-381 

scholarly journals New Diazo Coupling Reactions for Visible Spectrophotometric Determination of Alfuzosin in Pharmaceutical Preparations

2007 ◽  
Vol 4 (4) ◽  
pp. 496-501 ◽  
Author(s):  
M. Vamsi Krishna ◽  
D. Gowri Sankar
Keyword(s):  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Basic Medium ◽  
Coupling Reactions ◽  
Acetyl Acetone ◽  
Diazo Coupling ◽  
Optimum Reaction ◽  
Diazonium Ion

Simple, rapid and sensitive spectrophotometric procedures were developed for the analysis of Alfuzosin hydrochloride (AFZ) in pure form as well as in pharmaceutical formulations. The methods are based on the reaction of AFZ with nitrite in acid medium to form diazonium ion, which is coupled with ethoxyethylenemaleic ester (Method A) or ethylcyanoacetate (Method B) or acetyl acetone (method C) in basic medium to form azo dyes, showing absorption maxima at 440, 465 and 490 nm respectively. Beer’s law is obeyed in the concentration of 4-20 μg/mL of AFZ for methods A, B and 3-15 μg/mL of AFZ for method C. The molar absorptivity and sandell’s sensitivity of AFZ- ethoxyethylenemaleic ester, AFZ- ethylcyanoacetate and AFZ-acetyl acetone are1.90 × 104, 0.022; 1.93 × 104, 0.021 and 2.67 × 104L mole-1cm-1, 0.015 μg cm-2respectively. The optimum reaction conditions and other analytical parameters were evaluated. The methods were successfully applied to the determination of AFZ in pharmaceutical formulations.

scholarly journals Indirect spectrophotometric determination of gentamicin and vancomycin antibiotics based on their oxidation by potassium permanganate

2006 ◽  
Vol 4 (4) ◽  
pp. 708-722 ◽  
Author(s):  
Akram El-Didamony ◽  
Alaa Amin ◽  
Ahmed Ghoneim ◽  
Ayman Telebany
Keyword(s):  
Acid Medium ◽  
Potassium Permanganate ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Reaction Conditions ◽  
Subsequent Determination ◽  
Optimum Reaction ◽  
Acid Orange Ii ◽  
Sulphuric Acid Medium

AbstractFour simple, accurate, sensitive and economical procedures (A–D) for the estimation of gentamicin sulphate and vancomycin hydrochloride, both in pure form and in pharmaceutical formulations have been developed. The methods are based on the oxidation of the studied drugs by a known excess of potassium permanganate in sulphuric acid medium and subsequent determination of unreacted oxidant by reacting it with amaranth dye (method A), acid orange II (method B), indigocarmine (method C) and methylene blue (method D), in the same acid medium at a suitable λmax=521, 485, 610 and 664 nm, respectively. The reacted oxidant corresponds to the drug content. Regression analysis of Beer-Lambert plots showed good correlations in the concentration ranges 4–8, 3–8, 4–9 and 5–9 µg ml−1 with gentamicin and 4–8, 1.5–4, 1.5–4 and 3.5–5.5 µg ml−1 with vancomycin for methods A, B, C, and D, respectively. The molar absorptivity, sandell sensitivity, detection and quantification limits were calculated. The stoichiometric ratios for the cited drugs were studied. The optimum reaction conditions and other analytical parameters were evaluated. The influence of the substance commonly employed as excipients with these drugs were studied. The proposed methods were applied to the determination of these drugs in pharmaceutical formulations. The results have demonstrated that the methods are equally accurate and reproducible as the official methods.

scholarly journals Indirect spectrophotometric Indirect spectrophotometric determination of chlordiazepoxide in pharmaceutical formulations via oxidative coupling reaction with phenothiazine

2018 ◽  
pp. 7-14
Author(s):  
Hind Hadi ◽  
Mariam Jamal
Keyword(s):  
Oxidative Coupling ◽  
Coupling Reaction ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Calibration Plot ◽  
Pharmaceutical Dosage Forms ◽  
Oxidative Coupling Reaction ◽  
Formed Product

Abstract A sensitive, precise and reliable indirect spectrophotometric method for the determination of chlordiazepoxide (CDE) in pure and pharmaceutical dosage forms is described. The method is based on oxidative coupling reaction between amino group resulting from acidic decomposition of CDE with phenothiazine in the presence of sodium periodate to produce an intense green soluble dye that is stable and shows a maximum absorption at 602 nm. The calibration plot indicates that Beer’s law is obeyed over the concentration range of 0.1?50 µg/mL, with a molar absorptivity of 1×104 L/mol cm and correlation coefficient of 0.9994.All the conditions that affecting on the stability and sensitivity of the formed product were studied and optimized and the suggested method was effectively applied for the determination of CDE in commercial dosage forms.

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