scholarly journals Immunofluorescence-guided atomic force microscopy to measure the micromechanical properties of the pericellular matrix of porcine articular cartilage

2012 ◽  
Vol 9 (76) ◽  
pp. 2997-3007 ◽  
Author(s):  
Rebecca E. Wilusz ◽  
Louis E. DeFrate ◽  
Farshid Guilak
Keyword(s):  
Atomic Force Microscopy ◽  
Articular Cartilage ◽  
Elastic Moduli ◽  
Articular Surface ◽  
Biomechanical Properties ◽  
Pericellular Matrix ◽  
Type Vi Collagen ◽  
Force Microscopy ◽  
Micromechanical Properties ◽  
Atomic Force

The pericellular matrix (PCM) is a narrow region that is rich in type VI collagen that surrounds each chondrocyte within the extracellular matrix (ECM) of articular cartilage. Previous studies have demonstrated that the chondrocyte micromechanical environment depends on the relative properties of the chondrocyte, its PCM and the ECM. The objective of this study was to measure the influence of type VI collagen on site-specific micromechanical properties of cartilage in situ by combining atomic force microscopy stiffness mapping with immunofluorescence imaging of PCM and ECM regions in cryo-sectioned tissue samples. This method was used to test the hypotheses that PCM biomechanical properties correlate with the presence of type VI collagen and are uniform with depth from the articular surface. Control experiments verified that immunolabelling did not affect the properties of the ECM or PCM. PCM biomechanical properties correlated with the presence of type VI collagen, and matrix regions lacking type VI collagen immediately adjacent to the PCM exhibited higher elastic moduli than regions positive for type VI collagen. PCM elastic moduli were similar in all three zones. Our findings provide further support for type VI collagen in defining the chondrocyte PCM and contributing to its biological and biomechanical properties.

The Inhomogeneous Mechanical Properties of the Pericellular Matrix of Articular Cartilage Measured In Situ by Atomic Force Microscopy

2009 ◽  
Author(s):  
Rebecca E. Wilusz ◽  
Eric M. Darling ◽  
Michael P. Bolognesi ◽  
Stefan Zauscher ◽  
Farshid Guilak
Keyword(s):  
Mechanical Properties ◽  
Atomic Force Microscopy ◽  
Articular Cartilage ◽  
Biomechanical Properties ◽  
Human Articular Cartilage ◽  
Pericellular Matrix ◽  
Force Microscopy ◽  
Narrow Region ◽  
Atomic Force

Articular cartilage is the connective tissue that lines the articulating surfaces of diarthrodial joints, providing a low-friction, load-bearing surface during joint motion. Articular cartilage comprises of a single cell type, the chondrocyte, embedded within an extensive extracellular matrix (ECM). Each chondrocyte is surrounded by a narrow region called the pericellular matrix (PCM) that is distinct from the ECM in both its biochemical composition [1] and biomechanical properties [2]. While multiple techniques have been used to measure the mechanical properties of the PCM, including micropipette aspiration of isolated chondrons [2], these studies required mechanical or enzymatic extraction of the chondrocyte and surrounding PCM (i.e., the “chondron” [1]) from the cartilage, and the influence of this isolation process on PCM properties is unknown. Atomic force microscopy (AFM) provides a high resolution form of nano- and microindentation approaches that can be used to measure local mechanical properties in situ [3,4]. The objective of this study was to use AFM to quantify the biomechanical properties of the ECM and PCM of human articular cartilage in situ.

scholarly journals Spatial Mapping of the Biomechanical Properties of the Pericellular Matrix of Articular Cartilage Measured In Situ via Atomic Force Microscopy

2010 ◽  
Vol 98 (12) ◽  
pp. 2848-2856 ◽  
Author(s):  
Eric M. Darling ◽  
Rebecca E. Wilusz ◽  
Michael P. Bolognesi ◽  
Stefan Zauscher ◽  
Farshid Guilak
Keyword(s):  
Atomic Force Microscopy ◽  
Articular Cartilage ◽  
Biomechanical Properties ◽  
Spatial Mapping ◽  
Pericellular Matrix ◽  
Force Microscopy ◽  
Atomic Force

scholarly journals The measurement of biomechanical properties of porcine articular cartilage using atomic force microscopy

2009 ◽  
Vol 72 (4/5) ◽  
pp. 251-259 ◽  
Author(s):  
Raphael Imer ◽  
Terunobu Akiyama ◽  
Nico F. de Rooij ◽  
Martin Stolz ◽  
Ueli Aebi ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Articular Cartilage ◽  
Biomechanical Properties ◽  
Force Microscopy ◽  
Atomic Force

Local micromechanical properties of decellularized lung scaffolds measured with atomic force microscopy

2013 ◽  
Vol 9 (6) ◽  
pp. 6852-6859 ◽  
Author(s):  
T. Luque ◽  
E. Melo ◽  
E. Garreta ◽  
J. Cortiella ◽  
J. Nichols ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Micromechanical Properties ◽  
Atomic Force

Probing Micromechanical Properties of the Extracellular Matrix of Soft Tissues by Atomic Force Microscopy

2016 ◽  
Vol 232 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Ignasi Jorba ◽  
Juan J. Uriarte ◽  
Noelia Campillo ◽  
Ramon Farré ◽  
Daniel Navajas
Keyword(s):  
Extracellular Matrix ◽  
Atomic Force Microscopy ◽  
Soft Tissues ◽  
Force Microscopy ◽  
Micromechanical Properties ◽  
Atomic Force

Nanomechanical Probing of Layered Nanoscale Polymer Films With Atomic Force Microscopy

2004 ◽  
Vol 19 (3) ◽  
pp. 716-728 ◽  
Author(s):  
A. Kovalev ◽  
H. Shulha ◽  
M. Lemieux ◽  
N. Myshkin ◽  
V.V. Tsukruk
Keyword(s):  
Atomic Force Microscopy ◽  
Polymer Films ◽  
Elastic Moduli ◽  
Solid Substrate ◽  
Elastic Solids ◽  
Polymer Layers ◽  
Transition Zones ◽  
Force Microscopy ◽  
Microstructural Parameters ◽  
Atomic Force

The approach developed for the microindentation of layered elastic solids was adapted to analyze atomic force microscopy probing of ultrathin (1–100 nm-thick) polymer films on a solid substrate. The model for analyzing microindentation of layered solids was extended to construct two- and tri-step graded functions with the transition zones accounting for a variable gradient between layers. This “graded” approach offered a transparent consideration of the gradient of the mechanical properties between layers. Several examples of recent applications of this model to nanoscale polymer layers were presented. We considered polymer layers with elastic moduli ranging from 0.05 to 3000 MPa with different architecture in a dry state and in a solvated state. The most sophisticated case of a tri-layered polymer film with thickness of 20–50 nm was also successfully treated within this approach. In all cases, a complex shape of corresponding loading curves and elastic modulus depth profiles obtained from experimental data were fitted by the graded functions with nanomechanical parameters (elastic moduli and transition zone widths) close to independently determined microstructural parameters (thickness and composition of layers) of the layered materials.

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