Probing Micromechanical Properties of the Extracellular Matrix of Soft Tissues by Atomic Force Microscopy

2016 ◽  
Vol 232 (1) ◽  
pp. 19-26 ◽  
Author(s):  
Ignasi Jorba ◽  
Juan J. Uriarte ◽  
Noelia Campillo ◽  
Ramon Farré ◽  
Daniel Navajas
Keyword(s):  
Extracellular Matrix ◽  
Atomic Force Microscopy ◽  
Soft Tissues ◽  
Force Microscopy ◽  
Micromechanical Properties ◽  
Atomic Force

Local micromechanical properties of decellularized lung scaffolds measured with atomic force microscopy

2013 ◽  
Vol 9 (6) ◽  
pp. 6852-6859 ◽  
Author(s):  
T. Luque ◽  
E. Melo ◽  
E. Garreta ◽  
J. Cortiella ◽  
J. Nichols ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Micromechanical Properties ◽  
Atomic Force

Effect of ageing on micromechanical properties of bitumen by means of atomic force microscopy

2017 ◽  
Vol 18 (sup2) ◽  
pp. 203-215 ◽  
Author(s):  
José P Aguiar-Moya ◽  
Jorge Salazar-Delgado ◽  
Adriana García ◽  
Alejandra Baldi-Sevilla ◽  
Vivian Bonilla-Mora ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Force Microscopy ◽  
Micromechanical Properties ◽  
Atomic Force

scholarly journals Nanoscale characterization of effect of l-arginine on Streptococcus mutans biofilm adhesion by atomic force microscopy

Microbiology ◽  
2014 ◽  
Vol 160 (7) ◽  
pp. 1466-1473 ◽  
Author(s):  
Shivani Sharma ◽  
Stacey Lavender ◽  
JungReem Woo ◽  
Lihong Guo ◽  
Wenyuan Shi ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Atomic Force Microscopy ◽  
Streptococcus Mutans ◽  
Oral Microbiome ◽  
Aetiological Factor ◽  
Tooth Surface ◽  
Extracellular Polysaccharides ◽  
Adhesion Properties ◽  
Force Microscopy ◽  
Atomic Force

A major aetiological factor of dental caries is the pathology of the dental plaque biofilms. The amino acid l-arginine (Arg) is found naturally in saliva as a free molecule or as a part of salivary peptides and proteins. Plaque bacteria metabolize Arg to produce alkali and neutralize glycolytic acids, promoting a less cariogenous oral microbiome. Here, we explored an alternative and complementary mechanism of action of Arg using atomic force microscopy. The nanomechanical properties of Streptococcus mutans biofilm extracellular matrix were characterized under physiological buffer conditions. We report the effect of Arg on the adhesive behaviour and structural properties of extracellular polysaccharides in S. mutans biofilms. High-resolution imaging of biofilm surfaces can reveal additional structural information on bacterial cells embedded within the surrounding extracellular matrix. A dense extracellular matrix was observed in biofilms without Arg compared to those grown in the presence of Arg. S. mutans biofilms grown in the presence of Arg could influence the production and/or composition of extracellular membrane glucans and thereby affect their adhesion properties. Our results suggest that the presence of Arg in the oral cavity could influence the adhesion properties of S. mutans to the tooth surface.

scholarly journals Deconvolution of dissipative pathways for the interpretation of tapping-mode atomic force microscopy from phase-contrast

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Arindam Phani ◽  
Ho Sang Jung ◽  
Seonghwan Kim
Keyword(s):  
Atomic Force Microscopy ◽  
Phase Contrast ◽  
Soft Tissues ◽  
Loss Mechanism ◽  
Tapping Mode ◽  
Force Microscopy ◽  
Atomic Force ◽  
Single Cancer ◽  
Nanoscale Features ◽  
Mode Atomic Force Microscopy

AbstractPhase-contrast in tapping-mode atomic force microscopy (TM-AFM) results from dynamic tip-surface interaction losses which allow soft and hard nanoscale features to be distinguished. So far, phase-contrast in TM-AFM has been interpreted using homogeneous Boltzmann-like loss distributions that ignore fluctuations. Here, we revisit the origin of phase-contrast in TM-AFM by considering the role of fluctuation-driven transitions and heterogeneous loss. At ultra-light tapping amplitudes <3 nm, a unique amplitude dependent two-stage distribution response is revealed, alluding to metastable viscous relaxations that originate from tapping-induced surface perturbations. The elastic and viscous coefficients are also quantitatively estimated from the resulting strain rate at the fixed tapping frequency. The transitional heterogeneous losses emerge as the dominant loss mechanism outweighing homogeneous losses at smaller amplitudes for a soft-material. Analogous fluctuation mediated phase-contrast is also apparent in contact resonance enhanced AFM-IR (infrared), showing promise in decoupling competing thermal loss mechanisms via radiative and non-radiative pathways. Understanding the loss pathways can provide insights on the bio-physical origins of heterogeneities in soft-bio-matter e.g., single cancer cell, tumors, and soft-tissues.

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