scholarly journals The sensitivity of human mesenchymal stem cells to vibration and cold storage conditions representative of cold transportation

2012 ◽  
Vol 9 (75) ◽  
pp. 2503-2515 ◽  
Author(s):  
N. I. Nikolaev ◽  
Y. Liu ◽  
H. Hussein ◽  
D. J. Williams
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Cold Storage ◽  
Human Mesenchymal Stem Cells ◽  
Peak Acceleration ◽  
Trypan Blue Exclusion Method ◽  
Peak Displacement ◽  
Membrane Rupture

In the current study, the mechanical and hypothermic damage induced by vibration and cold storage on human mesenchymal stem cells (hMSCs) stored at 2–8°C was quantified by measuring the total cell number and cell viability after exposure to vibration at 50 Hz (peak acceleration 140 m s −2 and peak displacement 1.4 mm), 25 Hz (peak acceleration 140 m s −2 , peak displacement 5.7 mm), 10 Hz (peak acceleration 20 m s −2 , peak displacement 5.1 mm) and cold storage for several durations. To quantify the viability of the cells, in addition to the trypan blue exclusion method, the combination of annexin V-FITC and propidium iodide was applied to understand the mode of cell death. Cell granularity and a panel of cell surface markers for stemness, including CD29, CD44, CD105 and CD166, were also evaluated for each condition. It was found that hMSCs were sensitive to vibration at 25 Hz, with moderate effects at 50 Hz and no effects at 10 Hz. Vibration at 25 Hz also increased CD29 and CD44 expression. The study further showed that cold storage alone caused a decrease in cell viability, especially after 48 h, and also increased CD29 and CD44 and attenuated CD105 expressions. Cell death would most likely be the consequence of membrane rupture, owing to necrosis induced by cold storage. The sensitivity of cells to different vibrations within the mechanical system is due to a combined effect of displacement and acceleration, and hMSCs with a longer cold storage duration were more susceptible to vibration damage, indicating a coupling between the effects of vibration and cold storage.

Human mesenchymal stem cells protect neutrophils from serum-deprived cell death

2011 ◽  
Vol 35 (12) ◽  
pp. 1247-1251 ◽  
Author(s):  
Maryam Maqbool ◽  
Sharmili Vidyadaran ◽  
Elizabeth George ◽  
Rajesh Ramasamy
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

scholarly journals Encapsulin Based Self-Assembling Iron-Containing Protein Nanoparticles for Stem Cells MRI Visualization

2021 ◽  
Vol 22 (22) ◽  
pp. 12275
Author(s):  
Anna N. Gabashvili ◽  
Stepan S. Vodopyanov ◽  
Nelly S. Chmelyuk ◽  
Viktoria A. Sarkisova ◽  
Konstantin A. Fedotov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Human Mesenchymal Stem Cells ◽  
Noninvasive Technique ◽  
Self Assembling ◽  
Cargo Protein ◽  
Car T ◽  
New Type ◽  
Magnetic Resonance Imaging Mri

Over the past decade, cell therapy has found many applications in the treatment of different diseases. Some of the cells already used in clinical practice include stem cells and CAR-T cells. Compared with traditional drugs, living cells are much more complicated systems that must be strictly controlled to avoid undesirable migration, differentiation, or proliferation. One of the approaches used to prevent such side effects involves monitoring cell distribution in the human body by any noninvasive technique, such as magnetic resonance imaging (MRI). Long-term tracking of stem cells with artificial magnetic labels, such as magnetic nanoparticles, is quite problematic because such labels can affect the metabolic process and cell viability. Additionally, the concentration of exogenous labels will decrease during cell division, leading to a corresponding decrease in signal intensity. In the current work, we present a new type of genetically encoded label based on encapsulin from Myxococcus xanthus bacteria, stably expressed in human mesenchymal stem cells (MSCs) and coexpressed with ferroxidase as a cargo protein for nanoparticles’ synthesis inside encapsulin shells. mZip14 protein was expressed for the enhancement of iron transport into the cell. Together, these three proteins led to the synthesis of iron-containing nanoparticles in mesenchymal stem cells—without affecting cell viability—and increased contrast properties of MSCs in MRI.

scholarly journals An in vitro Evaluation of the Cytotoxicity of Varying Concentrations of Sodium Hypochlorite on Human Mesenchymal Stem Cells

2014 ◽  
Vol 15 (4) ◽  
pp. 473-481 ◽  
Author(s):  
Zeeshan H Ahmad ◽  
Sarah M Alkahtany ◽  
Sukumaran Anil
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Viability ◽  
Sodium Hypochlorite ◽  
Human Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Marrow Mesenchymal Stem Cells

ABSTRACT Aim To evaluate and compare the cytotoxicity of various concentrations of sodium hypochlorite on immortalized human bone marrow mesenchymal stem cells (MSCs). Materials and methods The 5.25 percent sodium hypochlorite (NaOCl) at concentrations of 0.5, 0.1, 0.025, 0.0125, and 0.005 mg/ml were used to assess the cytotoxic effect on MSCs. Immortalized human bone marrow mesenchymal stem cells (hTERT-MSCs) were exposed to NaOCl at 5 different concentrations. Cell viability was assessed by 3-(4, 5-dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide (MTT) and alamarBlue assays. The cell morphology changes were assessed with scanning electron microscopy (SEM) after exposure to 2, 4, and 24 hour incubation. The ethidium bromide/acridine orange (EB/ AO) fluorescent stain was applied to the cells in the 8-chamber slides after they were incubated with the testing agents for 2 and 4 hours to detect live and dead cells. The observations were quantitatively and qualitatively analyzed. Results The cell viability study using MTT assay and AB assay showed significant reduction with varying concentration at 2 and 4 hours incubation period. The cell viability decreased with the higher percentage of NaOCl. The exposure time also revealed an inverse relation to the cell viability. The SEM analysis showed reduction in the number of cells and morphological alterations with 0.5 mg/ml at 2 and 4 hours compared to 0.025 mg/ml NaOCl. Destruction of the cells with structural alterations and lysis was evident under fluorescence microscope when the cells were exposed to 0.5 mg/ml NaOCl. Conclusion Within the limitations of this in vitro study it can be concluded that NaOCl is toxic to the human bone marrow MSCs. The cell lysis was evident with higher concentration of sodium hypochlorite. From the observations, it can be concluded that a lower concentration of NaOCl may be used as endodontic irrigant due to its cytotoxic properties. Further studies are man datory to evolve a consensus on the optimal concentration of sodium hypochlorite to be used as endodontic irrigant. How to cite this article Alkahtani A, Alkahtany SM, Anil S. An in vitro Evaluation of the Cytotoxicity of Varying Concentrations of Sodium Hypochlorite on Human Mesenchymal Stem Cells. J Contemp Dent Pract 2014;15(4):473-481.

Al2O3Nanoparticles Induce Mitochondria-Mediated Cell Death and Upregulate the Expression of Signaling Genes in Human Mesenchymal Stem Cells

2012 ◽  
Vol 26 (11) ◽  
pp. 469-476 ◽  
Author(s):  
Ali A. Alshatwi ◽  
Periasamy Vaiyapuri Subbarayan ◽  
E. Ramesh ◽  
Amal A. Al-Hazzani ◽  
Mohammed A. Alsaif ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

scholarly journals Statin-induced calcification in human mesenchymal stem cells is cell death related

2009 ◽  
Vol 13 (11-12) ◽  
pp. 4465-4473 ◽  
Author(s):  
Laszlo Kupcsik ◽  
Thomas Meurya ◽  
Matthias Flury ◽  
Martin Stoddart ◽  
Mauro Alini
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells

scholarly journals Programmed cell death 4 inhibits proliferation and differentiation and induces apoptosis of human mesenchymal stem cells through suppressing the Wnt/β-catenin pathway

RSC Advances ◽  
2017 ◽  
Vol 7 (43) ◽  
pp. 26566-26573 ◽  
Author(s):  
Yang Liu ◽  
Dongyue Su ◽  
Tao Song
Keyword(s):  
Stem Cells ◽  
Cell Death ◽  
Mesenchymal Stem Cells ◽  
Programmed Cell Death ◽  
Human Mesenchymal Stem Cells ◽  
Proliferation And Differentiation ◽  
Programmed Cell Death 4

PDCD4 was closely related to the proliferation and the apoptosis of OP-hMSCs in osteoporosis.

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