scholarly journals New generation of magnetic and luminescent nanoparticles for in vivo real-time imaging

2013 ◽  
Vol 3 (3) ◽  
pp. 20120103 ◽  
Author(s):  
Lise-Marie Lacroix ◽  
Fabien Delpech ◽  
Céline Nayral ◽  
Sébastien Lachaize ◽  
Bruno Chaudret
Keyword(s):  
Contrast Agents ◽  
Real Time ◽  
Metallic Nanoparticles ◽  
Surface Engineering ◽  
Semiconductor Nanocrystals ◽  
Fluorescent Imaging ◽  
Real Time Imaging ◽  
Specific Targeting ◽  
New Generation

A new generation of optimized contrast agents is emerging, based on metallic nanoparticles (NPs) and semiconductor nanocrystals for, respectively, magnetic resonance imaging (MRI) and near-infrared (NIR) fluorescent imaging techniques. Compared with established contrast agents, such as iron oxide NPs or organic dyes, these NPs benefit from several advantages: their magnetic and optical properties can be tuned through size, shape and composition engineering, their efficiency can exceed by several orders of magnitude that of contrast agents clinically used, their surface can be modified to incorporate specific targeting agents and antifolding polymers to increase blood circulation time and tumour recognition, and they can possibly be integrated in complex architecture to yield multi-modal imaging agents. In this review, we will report the materials of choice based on the understanding of the basic physics of NIR and MRI techniques and their corresponding syntheses as NPs. Surface engineering, water transfer and specific targeting will be highlighted prior to their first use for in vivo real-time imaging. Highly efficient NPs that are safer and target specific are likely to enter clinical application in a near future.

scholarly journals Performance of a novel protease-activated fluorescent imaging system for intraoperative detection of residual breast cancer during breast conserving surgery

2021 ◽  
Vol 187 (1) ◽  
pp. 145-153
Author(s):  
Conor R. Lanahan ◽  
Bridget N. Kelly ◽  
Michele A. Gadd ◽  
Michelle C. Specht ◽  
Carson L. Brown ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Imaging System ◽  
Ex Vivo ◽  
Menopausal Status ◽  
Fluorescent Imaging ◽  
Cancer Subtypes ◽  
Surgical Workflow ◽  
Tumor Types

Abstract Purpose Safe breast cancer lumpectomies require microscopically clear margins. Real-time margin assessment options are limited, and 20–40% of lumpectomies have positive margins requiring re-excision. The LUM Imaging System previously showed excellent sensitivity and specificity for tumor detection during lumpectomy surgery. We explored its impact on surgical workflow and performance across patient and tumor types. Methods We performed IRB-approved, prospective, non-randomized studies in breast cancer lumpectomy procedures. The LUM Imaging System uses LUM015, a protease-activated fluorescent imaging agent that identifies residual tumor in the surgical cavity walls. Fluorescent cavity images were collected in real-time and analyzed using system software. Results Cavity and specimen images were obtained in 55 patients injected with LUM015 at 0.5 or 1.0 mg/kg and in 5 patients who did not receive LUM015. All tumor types were distinguished from normal tissue, with mean tumor:normal (T:N) signal ratios of 3.81–5.69. T:N ratios were 4.45 in non-dense and 4.00 in dense breasts (p = 0.59) and 3.52 in premenopausal and 4.59 in postmenopausal women (p = 0.19). Histopathology and tumor receptor testing were not affected by LUM015. Falsely positive readings were more likely when tumor was present < 2 mm from the adjacent specimen margin. LUM015 signal was stable in vivo at least 6.5 h post injection, and ex vivo at least 4 h post excision. Conclusions Intraoperative use of the LUM Imaging System detected all breast cancer subtypes with robust performance independent of menopausal status and breast density. There was no significant impact on histopathology or receptor evaluation.

scholarly journals Quantitative real-time imaging of molecular dynamics during cancer cell invasion and metastasis in vivo

2009 ◽  
Vol 3 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Paul Timpson ◽  
Alan Serrels ◽  
Marta Canel ◽  
Margaret C. Frame ◽  
Valerie G. Brunton ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Real Time ◽  
Cancer Cell ◽  
Cell Invasion ◽  
Cancer Cell Invasion ◽  
Invasion And Metastasis ◽  
Real Time Imaging

A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo

2013 ◽  
Vol 4 (3) ◽  
pp. 1079 ◽  
Author(s):  
Kehua Xu ◽  
Mingming Qiang ◽  
Wen Gao ◽  
Ruixian Su ◽  
Na Li ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Redox Status ◽  
Real Time Imaging

Real time imaging reveals a peroxisomal reticulum in living cells

2000 ◽  
Vol 113 (20) ◽  
pp. 3663-3671 ◽  
Author(s):  
M. Schrader ◽  
S.J. King ◽  
T.A. Stroh ◽  
T.A. Schroer
Keyword(s):  
Real Time ◽  
Cytoplasmic Dynein ◽  
Living Cells ◽  
Real Time Imaging ◽  
Peroxisomal Targeting ◽  
Microtubule Motor ◽  
Targeting Signal ◽  
Peroxisomal Targeting Signal

We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.

476 ICG FLUORESCENCE IN IDENTIFICATION OF THORACIC DUCT

2021 ◽  
Vol 34 (Supplement_1) ◽  
Author(s):  
Subramanyeshwar Rao Thammineedi
Keyword(s):  
Small Bowel ◽  
Real Time ◽  
Thoracic Duct ◽  
Fluorescent Imaging ◽  
Small Bowel Mesentery ◽  
Video Presentation ◽  
Real Time Imaging ◽  
Intraoperative Fluorescence

Abstract   Not applicable because it is a Video presentation. Methods Not applicable because it is a Video presentation. Results Not applicable because it is a Video presentation. Conclusion Not applicable because it is a Video presentation. Video Video shows visualisation of thoracic duct during esophagectomy by real-time fluorescent imaging. Intraoperative fluorescence lymphangiography was performed by injecting 2.5 mg of ICG into small bowel mesentery at Laparoscopy.At Thoracoscopy, Thoracic duct was visualised after 65 minutes. ICG fluorescence lymphangiography provides a feasible, reliable and real-time imaging of thoracic duct during esophagectomy, thereby potentially reducing thoracic duct injuries. https://drive.google.com/open?id=1ROGQ1K-yWkO-B3oSk8dGbsRu4iCKGfJq.

Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo

2006 ◽  
Author(s):  
Takashi Sakurai ◽  
Seiji Yamamoto ◽  
Atsuo Miyakawa ◽  
Yoshihiko Wakazono ◽  
Takato O. Yoshida ◽  
...  
Keyword(s):  
Real Time ◽  
Confocal Microscope ◽  
Real Time Imaging

scholarly journals High-Speed, High-Performance Real-Time Imaging of Physiological Sarcomere Dynamics in the Beating Mouse Heart in Vivo

2016 ◽  
Vol 110 (3) ◽  
pp. 463a
Author(s):  
Fuyu Kobirumaki-Shimozawa ◽  
Kotaro Oyama ◽  
Togo Shimozawa ◽  
Takashi Ohki ◽  
Takako Terui ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
High Performance ◽  
Mouse Heart ◽  
Real Time Imaging ◽  
Sarcomere Dynamics

scholarly journals S36 Check novel in vivo real time imaging of the bronchial mucosa using an endo-cytoscopy

Thorax ◽  
2010 ◽  
Vol 65 (Suppl 4) ◽  
pp. A18-A19
Author(s):  
K. Shibuya ◽  
N. Okada ◽  
H. Kohno ◽  
N. Iwai ◽  
M. Noro ◽  
...  
Keyword(s):  
Real Time ◽  
Bronchial Mucosa ◽  
Real Time Imaging

Reflectance and fluorescent contrast agents for real-time in vivo confocal imaging

2000 ◽  
Author(s):  
Milind Rajadhyaksha ◽  
Mark Henrichs ◽  
K. P. Ananth ◽  
Hung-Ta Chang ◽  
Salvador González
Keyword(s):  
Contrast Agents ◽  
Real Time ◽  
Confocal Imaging

scholarly journals In vivo real-time imaging of TGF-?-induced transcriptional activation of the RANK ligand gene promoter in intraosseous prostate cancer

The Prostate ◽  
2004 ◽  
Vol 59 (4) ◽  
pp. 360-369 ◽  
Author(s):  
Jian Zhang ◽  
Yi Lu ◽  
Jinlu Dai ◽  
Zhi Yao ◽  
Riko Kitazawa ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Transcriptional Activation ◽  
Gene Promoter ◽  
Rank Ligand ◽  
Real Time Imaging
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