scholarly journals In vivo real-time imaging of TGF-?-induced transcriptional activation of the RANK ligand gene promoter in intraosseous prostate cancer

The Prostate ◽  
2004 ◽  
Vol 59 (4) ◽  
pp. 360-369 ◽  
Author(s):  
Jian Zhang ◽  
Yi Lu ◽  
Jinlu Dai ◽  
Zhi Yao ◽  
Riko Kitazawa ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Transcriptional Activation ◽  
Gene Promoter ◽  
Rank Ligand ◽  
Real Time Imaging

scholarly journals Quantitative real-time imaging of molecular dynamics during cancer cell invasion and metastasis in vivo

2009 ◽  
Vol 3 (4) ◽  
pp. 351-354 ◽  
Author(s):  
Paul Timpson ◽  
Alan Serrels ◽  
Marta Canel ◽  
Margaret C. Frame ◽  
Valerie G. Brunton ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Real Time ◽  
Cancer Cell ◽  
Cell Invasion ◽  
Cancer Cell Invasion ◽  
Invasion And Metastasis ◽  
Real Time Imaging

A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo

2013 ◽  
Vol 4 (3) ◽  
pp. 1079 ◽  
Author(s):  
Kehua Xu ◽  
Mingming Qiang ◽  
Wen Gao ◽  
Ruixian Su ◽  
Na Li ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescent Probe ◽  
Near Infrared ◽  
Redox Status ◽  
Real Time Imaging

scholarly journals The circRAB3IP Mediated by eIF4A3 and LEF1 Contributes to Enzalutamide Resistance in Prostate Cancer by Targeting miR-133a-3p/miR-133b/SGK1 Pathway

2021 ◽  
Vol 11 ◽  
Author(s):  
Dong Chen ◽  
Yaqin Wang ◽  
Feiya Yang ◽  
Adili Keranmu ◽  
Qingxin Zhao ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Expression Pattern ◽  
Real Time Pcr ◽  
Bioinformatic Analysis ◽  
Biological Functions ◽  
Quantitative Real Time Pcr ◽  
Regulatory Effects

An increasing number of studies have shown that circRNAs are closely related to the carcinogenesis and development of prostate cancer (PCa). However, little is known about the effect of the biological functions of circRNAs on the enzalutamide resistance of PCa. Through bioinformatic analysis and experiments, we investigated the expression pattern of circRNAs in enzalutamide-resistant PCa cells. Quantitative real-time PCR was used to detect the expression of circRAB3IP, and plasmids that knock down or overexpress circRAB3IP were used to evaluate its effect on the enzalutamide sensitivity of PCa cells. Mechanistically, we explored the potential regulatory effects of eIF4A3 and LEF1 on the biogenesis of circRAB3IP. Our in vivo and in vitro data indicated that increased expression of circRAB3IP was found in enzalutamide-resistant PCa, and knockdown of circRAB3IP significantly enhanced enzalutamide sensitivity in PCa cells. However, upregulation of circRAB3IP resulted in the opposite effects. Further mechanistic research demonstrated that circRAB3IP could regulate the expression of serum and glucocorticoid-regulated kinase 1 (SGK1) by serving as a sponge that directly targets miR-133a-3p/miR-133b. Then, we showed that circRAB3IP partially exerted its biological functions via SGK1 signaling. Furthermore, we discovered that eIF4A3 and LEF1 might increase circRAB3IP expression in PCa.

scholarly journals Synthesis of Fluorescent Polythiophene Dots

2018 ◽  
Vol 2018 ◽  
pp. 1-7
Author(s):  
Zhen Liu ◽  
Alaa Nahhas ◽  
Li Liu ◽  
Earl Ada ◽  
Xinyu Zhang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Hydrazine Hydrate ◽  
Cancer Cells ◽  
Real Time ◽  
Fluorescence Imaging ◽  
Chemical Oxidation ◽  
Prostate Cancer Cells ◽  
One Step ◽  
Live Mouse

Ring-functionalized semiconducting polythiophene dots (Pdots) were synthesized rapidly and in one step by the hydrazine hydrate reduction of doped parent polythiophene, obtained by conventional chemical oxidation of thiophene monomer by FeCl3 in anhydrous acetonitrile. Dispersions of these Pdots display robust (pseudo) solvatochromism and solvatofluorism. Polythiophene Pdots exhibit significant cytotoxicity towards prostate cancer cells (expected) although when injected subcutaneously in vivo in live mouse, no toxicity is observed for 24 days when monitored in real time using fluorescence imaging.

Real time imaging reveals a peroxisomal reticulum in living cells

2000 ◽  
Vol 113 (20) ◽  
pp. 3663-3671 ◽  
Author(s):  
M. Schrader ◽  
S.J. King ◽  
T.A. Stroh ◽  
T.A. Schroer
Keyword(s):  
Real Time ◽  
Cytoplasmic Dynein ◽  
Living Cells ◽  
Real Time Imaging ◽  
Peroxisomal Targeting ◽  
Microtubule Motor ◽  
Targeting Signal ◽  
Peroxisomal Targeting Signal

We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.

Fiber-coupled confocal microscope (FCM) for real time imaging of cellular signals in vivo

2006 ◽  
Author(s):  
Takashi Sakurai ◽  
Seiji Yamamoto ◽  
Atsuo Miyakawa ◽  
Yoshihiko Wakazono ◽  
Takato O. Yoshida ◽  
...  
Keyword(s):  
Real Time ◽  
Confocal Microscope ◽  
Real Time Imaging

scholarly journals High-Speed, High-Performance Real-Time Imaging of Physiological Sarcomere Dynamics in the Beating Mouse Heart in Vivo

2016 ◽  
Vol 110 (3) ◽  
pp. 463a
Author(s):  
Fuyu Kobirumaki-Shimozawa ◽  
Kotaro Oyama ◽  
Togo Shimozawa ◽  
Takashi Ohki ◽  
Takako Terui ◽  
...  
Keyword(s):  
Real Time ◽  
High Speed ◽  
High Performance ◽  
Mouse Heart ◽  
Real Time Imaging ◽  
Sarcomere Dynamics

scholarly journals S36 Check novel in vivo real time imaging of the bronchial mucosa using an endo-cytoscopy

Thorax ◽  
2010 ◽  
Vol 65 (Suppl 4) ◽  
pp. A18-A19
Author(s):  
K. Shibuya ◽  
N. Okada ◽  
H. Kohno ◽  
N. Iwai ◽  
M. Noro ◽  
...  
Keyword(s):  
Real Time ◽  
Bronchial Mucosa ◽  
Real Time Imaging

Development of a suction device for stabilizing in vivo real-time imaging of murine tissues

2011 ◽  
Vol 112 (5) ◽  
pp. 508-510 ◽  
Author(s):  
Kazunori Shimizu ◽  
Yuriko Higuchi ◽  
Yoshimasa Kozu ◽  
Mitsuru Hashida ◽  
Satoshi Konishi
Keyword(s):  
Real Time ◽  
Suction Device ◽  
Real Time Imaging
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