scholarly journals Blocking CTGF/CCN2 reduces established skeletal muscle fibrosis in a rat model of overuse injury

2020 ◽  
Vol 34 (5) ◽  
pp. 6554-6569 ◽  
Author(s):  
Mary F. Barbe ◽  
Brendan A. Hilliard ◽  
Mamta Amin ◽  
Michele Y. Harris ◽  
Lucas J. Hobson ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rat Model ◽  
Overuse Injury ◽  
Muscle Fibrosis ◽  
Skeletal Muscle Fibrosis

scholarly journals Skeletal muscle fibrosis and stiffness increase after rotator cuff tendon injury and neuromuscular compromise in a rat model

2014 ◽  
Vol 32 (9) ◽  
pp. 1111-1116 ◽  
Author(s):  
Eugene J. Sato ◽  
Megan L. Killian ◽  
Anthony J. Choi ◽  
Evie Lin ◽  
Mary C. Esparza ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Rotator Cuff ◽  
Rat Model ◽  
Tendon Injury ◽  
Rotator Cuff Tendon ◽  
Muscle Fibrosis ◽  
Skeletal Muscle Fibrosis

Skeletal muscle fibrosis: the effect of stromal-derived factor-1α-loaded collagen scaffolds

2010 ◽  
Vol 5 (5) ◽  
pp. 737-747 ◽  
Author(s):  
Sander Grefte ◽  
Anne Marie Kuijpers-Jagtman ◽  
Ruurd Torensma ◽  
Johannes W Von den Hoff
Keyword(s):  
Skeletal Muscle ◽  
Collagen Scaffolds ◽  
Muscle Fibrosis ◽  
Skeletal Muscle Fibrosis

Effects of Geniposide from Gardenia Fruit Pomace on Skeletal-Muscle Fibrosis

2018 ◽  
Vol 66 (23) ◽  
pp. 5802-5811 ◽  
Author(s):  
Haiou Pan ◽  
Yan Li ◽  
Haifeng Qian ◽  
Xiguang Qi ◽  
Gangcheng Wu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibrosis ◽  
Fruit Pomace ◽  
Skeletal Muscle Fibrosis

Denervation-induced skeletal muscle fibrosis is mediated by CTGF/CCN2 independently of TGF-β

2019 ◽  
Vol 82 ◽  
pp. 20-37 ◽  
Author(s):  
Daniela L. Rebolledo ◽  
David González ◽  
Jennifer Faundez-Contreras ◽  
Osvaldo Contreras ◽  
Carlos P. Vio ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibrosis ◽  
Skeletal Muscle Fibrosis

scholarly journals Influence of Platelet Rich Plasma on the Skeletal Muscle Fibrosis after Limb Lengthening in Mice

2020 ◽  
Vol 5 (4) ◽  
pp. 2473011420S0046
Author(s):  
Ichiro Tonogai ◽  
Ichiro Tonogai
Keyword(s):  
Skeletal Muscle ◽  
Gastrocnemius Muscle ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Limb Lengthening ◽  
Major Constituent ◽  
Tibial Osteotomy ◽  
Muscle Fibrosis ◽  
Skeletal Muscle Fibrosis ◽  
Phosphate Buffered Saline

Category: Basic Sciences/Biologics Introduction/Purpose: Skeletal muscle fibrosis induced by the increase of collagen occurs after limb lengthening which is also called distraction osteogenesis. Although there are studies about influence of platelet rich plasma (PRP) on tissues healing process, its effectiveness is still controversial. The aim of this study was to examine whether PRP decreased the skeletal muscle fibrosis induced by limb lengthening. Methods: Tibial osteotomy was done to 8-week-old wild type mice. Tibia was lengthened at a rate of 0.42 mm/day during 2 weeks, launching 1 week after tibial osteotomy. Just after lengthening completed (3 weeks after tibial osteotomy), PRP was injected into the gastrocnemius muscle (PRP group). As a sham group, phosphate buffered saline (PBS) was injected into the gastrocnemius muscle (non-PRP group). The gastrocnemius (GC) muscles were taken and analyzed at 4, 6, 8 and 10 weeks after tibial osteotomy. Results: The fibrotic area of the GC muscles in the both groups increased at 4 weeks after tibial osteotomy in histological analysis (Figure). Then, it gradually decreased at 6, 8, and 10 weeks after tibial osteotomy. There were no significant differences between the both groups at 6, 8, and 10 weeks after tibial osteotomy. Hydroxyproline, which was a major constituent of collagen, increased in the non-PRP and PRP groups by limb lengthening as well. However, significant changes were not found between the both groups at all any points. Conclusion: At first, we anticipated that PRP should reduce the skeletal muscle fibrosis after limb lengthening significantly. But our results implied that PRP did not decrease the skeletal muscle fibrosis induced by limb lengthening.

scholarly journals Anti-Fibrotic Effect of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells on Skeletal Muscle Cells, Mediated by Secretion of MMP-1

2020 ◽  
Vol 21 (17) ◽  
pp. 6269
Author(s):  
Alee Choi ◽  
Sang Eon Park ◽  
Jang Bin Jeong ◽  
Suk-joo Choi ◽  
Soo-young Oh ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Wharton’S Jelly ◽  
Mdx Mice ◽  
Therapeutic Effects ◽  
Paracrine Factors ◽  
Wharton's Jelly ◽  
Muscle Fibrosis ◽  
Skeletal Muscle Fibrosis

Extracellular matrix (ECM) components play an important role in maintaining skeletal muscle function, but excessive accumulation of ECM components interferes with skeletal muscle regeneration after injury, eventually inducing fibrosis. Increased oxidative stress level caused by dystrophin deficiency is a key factor in fibrosis in Duchenne muscular dystrophy (DMD) patients. Mesenchymal stem cells (MSCs) are considered a promising therapeutic agent for various diseases involving fibrosis. In particular, the paracrine factors secreted by MSCs play an important role in the therapeutic effects of MSCs. In this study, we investigated the effects of MSCs on skeletal muscle fibrosis. In 2–5-month-old mdx mice intravenously injected with 1 × 105 Wharton’s jelly (WJ)-derived MSCs (WJ-MSCs), fibrosis intensity and accumulation of calcium/necrotic fibers were significantly decreased. To elucidate the mechanism of this effect, we verified the effect of WJ-MSCs in a hydrogen peroxide-induced fibrosis myotubes model. In addition, we demonstrated that matrix metalloproteinase-1 (MMP-1), a paracrine factor, is critical for this anti-fibrotic effect of WJ-MSCs. These findings demonstrate that WJ-MSCs exert anti-fibrotic effects against skeletal muscle fibrosis, primarily via MMP-1, indicating a novel target for the treatment of muscle diseases, such as DMD.

Sign in / Sign up

Export Citation Format

Share Document