Epidermal stem cells arise from the hair follicle after wounding

2007 ◽  
Vol 21 (7) ◽  
pp. 1358-1366 ◽  
Author(s):  
Vered Levy ◽  
Catherine Lindon ◽  
Ying Zheng ◽  
Brian D. Harfe ◽  
Bruce A. Morgan
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Epidermal Stem Cells

scholarly journals Hair follicle epidermal stem cells define a niche for tactile sensation

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Chun-Chun Cheng ◽  
Ko Tsutsui ◽  
Toru Taguchi ◽  
Noriko Sanzen ◽  
Asako Nakagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Stem Cell Niche ◽  
Epidermal Stem Cell ◽  
Epidermal Stem Cells ◽  
Hair Cycle ◽  
Unit Formation ◽  
Common Principle ◽  
Regeneration Cycle ◽  
Cell Niche

The heterogeneity and compartmentalization of stem cells is a common principle in many epithelia, and is known to function in epithelial maintenance, but its other physiological roles remain elusive. Here we show transcriptional and anatomical contributions of compartmentalized epidermal stem cells in tactile sensory unit formation in the mouse hair follicle. Epidermal stem cells in the follicle upper-bulge, where mechanosensory lanceolate complexes innervate, express a unique set of extracellular matrix (ECM) and neurogenesis-related genes. These epidermal stem cells deposit an ECM protein called EGFL6 into the collar matrix, a novel ECM that tightly ensheathes lanceolate complexes. EGFL6 is required for the proper patterning, touch responses, and αv integrin-enrichment of lanceolate complexes. By maintaining a quiescent original epidermal stem cell niche, the old bulge, epidermal stem cells provide anatomically stable follicle–lanceolate complex interfaces, irrespective of the stage of follicle regeneration cycle. Thus, compartmentalized epidermal stem cells provide a niche linking the hair follicle and the nervous system throughout the hair cycle.

scholarly journals Pre-aggregation of scalp progenitor dermal and epidermal stem cells activates the WNT pathway and promotes hair follicle formation in in vitro and in vivo systems

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Yiqun Su ◽  
Jie Wen ◽  
Junrong Zhu ◽  
Zhiwei Xie ◽  
Chang Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Progenitor Cells ◽  
Wnt Pathway ◽  
Three Dimensional ◽  
Epidermal Cells ◽  
Type I ◽  
Epidermal Stem Cells

Abstract Background Billions of dollars are invested annually by pharmaceutical companies in search of new options for treating hair loss conditions; nevertheless, the challenge remains. One major limitation to hair follicle research is the lack of effective and efficient drug screening systems using human cells. Organoids, three-dimensional in vitro structures derived from stem cells, provide new opportunities for studying organ development, tissue regeneration, and disease pathogenesis. The present study focuses on the formation of human hair follicle organoids. Methods Scalp-derived dermal progenitor cells mixed with foreskin-derived epidermal stem cells at a 2:1 ratio aggregated in suspension to form hair follicle-like organoids, which were confirmed by immunostaining of hair follicle markers and by molecular dye labeling assays to analyze dermal and epidermal cell organization in those organoids. The hair-forming potential of organoids was examined using an in vivo transplantation assay. Results Pre-aggregation of dermal and epidermal cells enhanced hair follicle formation in vivo. In vitro pre-aggregation initiated the interactions of epidermal and dermal progenitor cells resulting in activation of the WNT pathway and the formation of pear-shape structures, named type I aggregates. Cell-tracing analysis showed that the dermal and epidermal cells self-assembled into distinct epidermal and dermal compartments. Histologically, the type I aggregates expressed early hair follicle markers, suggesting the hair peg-like phase of hair follicle morphogenesis. The addition of recombinant WNT3a protein to the medium enhanced the formation of these aggregates, and the Wnt effect could be blocked by the WNT inhibitor, IWP2. Conclusions In summary, our system supports the rapid formation of a large number of hair follicle organoids (type I aggregates). This system provides a platform for studying epithelial-mesenchymal interactions, for assessing inductive hair stem cells and for screening compounds that support hair follicle regeneration.

scholarly journals Hair Follicle Epidermal Stem Cells Define a Niche for Tactile Sensation

2018 ◽  
Author(s):  
Chun-Chun Cheng ◽  
Ko Tsutsui ◽  
Toru Taguchi ◽  
Noriko Sanzen ◽  
Asako Nakagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Nervous System ◽  
Hair Follicle ◽  
Tactile Sensation ◽  
Epidermal Stem Cells ◽  
Hair Cycle ◽  
Unit Formation ◽  
Common Principle ◽  
Regeneration Cycle

AbstractThe heterogeneity and compartmentalization of stem cells is a common principle in many epithelia, and is known to function in epithelial maintenance, but its other physiological roles remain elusive. Here we show transcriptional and anatomical contributions of compartmentalized epidermal stem cells (EpSCs) in tactile sensory unit formation in the hair follicle (HF). EpSCs in the follicle upper-bulge, where mechanosensory lanceolate complexes (LCs) innervate, express a unique set of extracellular matrix (ECM) and neurogenesis-related genes. These EpSCs deposit an ECM protein EGFL6 into the collar matrix, a novel ECM that tightly ensheathes LCs. EGFL6 is required for the proper patterning, touch responses, and αv integrin-enrichment of LCs. By maintaining a quiescent original EpSC niche, the old bulge, EpSCs provide anatomically stable HF-LC interfaces, irrespective of the stage of follicle regeneration cycle. Thus, compartmentalized EpSCs provide a niche linking the HF and the nervous system throughout the hair cycle.

scholarly journals 174 ISOLATION, CULTURE AND POTENTIAL USE OF THE PORCINE NEURAL AND EPIDERMAL STEM CELLS

2005 ◽  
Vol 17 (2) ◽  
pp. 238
Author(s):  
J. Motlik ◽  
P. Vodicka ◽  
J. Klima ◽  
K. Smetana ◽  
F. Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Therapeutic Potential ◽  
Hair Follicles ◽  
Mammalian Brain ◽  
Epidermal Stem Cells ◽  
Feeder Cells ◽  
Ki 67 ◽  
Potential Marker

The mammalian brain and epidermis contain stem cells, so-called neural stem cells (NSC) and epidermal stem cells (EpSC). To achieve the full therapeutic potential of stem cells, appropriate animal models have to be used to establish the sequence of pathological changes and to test potential therapies to block these changes. In the following studies miniature pigs were used as a biomedical model. We isolated multipotent cells from brains of porcine fetuses for future use in allotransplantation experiments in the inbred miniature pig strain. Brain tissue from 40- and 80-day-old porcine fetuses was mechanically dissociated, and cells were cultured in serum-free F12/DMEM medium with B27 and N2 supplements, EGF and bFGF. In 3–5 days some cells divided and formed floating spheres that were dissociated to single cell suspension and formed secondary spheres in culture. At all time points tested, the spheres represented mixtures of undifferentiated cells stained with nestin and Ki-67 antibodies and already differentiated neurons (Tu-20, MAP2) and glia (GFAP). After being plated on laminin/fibronectin coated coverslips and cultured in medium containing 2% FBS or 1 μM retinoic acid, the spheres adhered to the surface, and flattened, and cells started to migrate out. After immunofluorescence staining with antibodies to neuronal markers Tu-20 and MAP2, glial marker GFAP and oligodendrocyte marker CNPase showed that all the three cell types were present among differentiated cells. The EpSC are characterized by a slow and unlimited proliferation rate and, therefore, they retain labelled precursors of DNA more extensively than other keratinocytes. The main pool of EpSC is located in the bulge region of the hair follicle root sheath. A new procedure to isolate porcine hair follicles including their root sheaths was developed. The keratinocytes that migrated from hair follicles in the presence of feeder cells were poorly differentiated and specifically expressed galectin-1 or galectin-1-binding sites in their nuclei in co-localization with ΔNp63α. The exclusion of feeder cells from experimental system induced formation of spheroid bodies from these keratinocytes. Approximately one-third of these spheroids were able to adhere to a surface precolonized with feeder cells and to start forming normally growing colonies. Porcine hair follicles represent an excellent model for study of the functional phenotype of hair follicle-originated keratinocytes, and the endogenous lectin Gal-1 seems to be a potential marker of the porcine stem cell compartment of the hair follicle under in vitro conditions.

scholarly journals Author response: Hair follicle epidermal stem cells define a niche for tactile sensation

2018 ◽  
Author(s):  
Chun-Chun Cheng ◽  
Ko Tsutsui ◽  
Toru Taguchi ◽  
Noriko Sanzen ◽  
Asako Nakagawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hair Follicle ◽  
Author Response ◽  
Tactile Sensation ◽  
Epidermal Stem Cells
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