Acquired Tolerance to Blasticidin-S in Pyricularia oryzae

1977 ◽  
Vol 77 (3) ◽  
pp. 421 ◽  
Author(s):  
Byung Kook Hwang
Keyword(s):  
Pyricularia Oryzae ◽  
Blasticidin S

Молекулярное маркирование генов устойчивости к пирикуляриозу в сортах риса дальневосточной селекции

2020 ◽  
pp. 30-33
Author(s):  
Марина Владиславовна Илюшко ◽  
Марина Викторовна Ромашова ◽  
Светлана Сергеевна Гученко
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Pyricularia Oryzae

С помощью молекулярно-генетических маркеров изучены 13 сортов риса (Oryza sativa L.), зарегистрированных в Государственном реестре селекционных достижений по дальневосточной зоне, и 1 перспективный сорт на наличие 6 генов устойчивости к пирикуляриозу риса — Pi-2, Pi-9, Pi-b, Pi-z(t), Pi-1 и Pi-ta2, наиболее актуальных для региона. В 4 сортах не обнаружено генов резистентности к пирикуляриозу. В 8 сортах идентифицировано по 1 гену устойчивости — Pi-2 и Pi-ta2. В 1 сорте (Садко) выявлено 2 изученных гена — Pi-z(t) и Pi-ta2. Изученные 8 сортов риса используют для технологии пирамидирования генов в селекции на устойчивость риса к пирикуляриозу для дальневосточной зоны рисосеяния. Выявленные гены резистентности риса к Pyricularia oryzae Cav. могут служить объективным идентификатором при паспортизации изученных сортов. Обсуждаются причины потери иммунности сортов и необходимости молекулярно-генетического сопровождения процесса семеноводства риса.

ASPECTOS BIOMÉTRICOS, GRAU DE UMIDADE EM SEMENTES DE SUCUPIRA E DETERMINAÇÃO DO EFEITO FUNGITÓXICO DO SEU ÓLEO ESSENCIAL

2019 ◽  
pp. 17-24
Author(s):  
Warlyton Silva Martins ◽  
Cid Tacaoca Muraishi
Keyword(s):  
Pyricularia Oryzae ◽  
Didymella Bryoniae ◽  
Fusarium Subglutinans

A busca por métodos naturais que viabilizam a eficiência da atividade biológica sobre vários microrganismos vem sendo destaque no cenário atual. A exploração da atividade biológica de compostos secundários presentes no extrato bruto ou óleos essenciais de plantas pode constituir, ao lado da indução da resistência, em uma forma efetiva de controle de doenças em plantas cultivadas. Diante disso, o presente estudo teve por objetivo avaliar a eficiência do óleo essencial de sucupira em suas propriedades de rendimento, bem como sua atividade biológica antifúngica sobre determinados patógenos que afetam culturas promissoras no Estado do Tocantins. Instalou-se o experimento no Campus de Ciências Agrárias da Faculdade Católica do Tocantins no laboratório de Bromatologia e Fitopatologia, para extração do óleo essência, avaliação do grau de umidade e biometria de sementes, obtenção dos fitopatógenos e avaliação dos efeitos fungitóxicos do óleo essencial. O delineamento experimental utilizado foi inteiramente casualizado com quatro tratamentos e cinco repetições. Os tratamentos, adicionados ao meio de cultura, após a autoclavagem e o resfriamento, foram as seguintes: sucupira (BDA + 25% de extrato de sucupira + 0,016% de polissorbato), sucupira (BDA + 50% de extrato de sucupira + 0,016% de polissorbato), sucupira (BDA + 75% de extrato de sucupira + 0,016% de polissorbato), sucupira (BDA + 100% de extrato de sucupira + 0,016% de polissorbato) e testemunha (BDA). Os resultados foram submetidos ao teste Tukey (5%) de probabilidade. O óleo essencial de sucupira inibiu o desenvolvimento micelial de Fusarium subglutinans, Sclerotinia sclerotiurum, Didymella bryoniae, Pyricularia oryzae.

Antifungal and Antimitotic Substances discovered by the Bioassay using Conidia of Pyricularia Oryzae

2004 ◽  
Vol 1 (3) ◽  
pp. 257-271 ◽  
Author(s):  
M. Namikoshi ◽  
H. Kobayashi ◽  
H. Liu ◽  
X. Yao ◽  
H. Zhang
Keyword(s):  
Pyricularia Oryzae

scholarly journals Copy number-dependent DNA methylation of the Pyricularia oryzae MAGGY retrotransposon is triggered by DNA damage

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Ba Van Vu ◽  
Quyet Nguyen ◽  
Yuki Kondo-Takeoka ◽  
Toshiki Murata ◽  
Naoki Kadotani ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Copy Number ◽  
Rna Silencing ◽  
Molecular Mechanisms ◽  
De Novo ◽  
Pyricularia Oryzae ◽  
Transcriptional Gene Silencing ◽  
Physical Interaction ◽  
Uncharacterized Protein ◽  
Form Complex

AbstractTransposable elements are common targets for transcriptional and post-transcriptional gene silencing in eukaryotic genomes. However, the molecular mechanisms responsible for sensing such repeated sequences in the genome remain largely unknown. Here, we show that machinery of homologous recombination (HR) and RNA silencing play cooperative roles in copy number-dependent de novo DNA methylation of the retrotransposon MAGGY in the fungusPyricularia oryzae. Genetic and physical interaction studies revealed thatRecAdomain-containing proteins, includingP. oryzaehomologs ofRad51, Rad55, andRad57, together with an uncharacterized protein, Ddnm1, form complex(es) and mediate either the overall level or the copy number-dependence of de novo MAGGY DNA methylation, likely in conjunction with DNA repair. Interestingly,P. oryzaemutants of specific RNA silencing components (MoDCL1andMoAGO2)were impaired in copy number-dependence of MAGGY methylation. Co-immunoprecipitation of MoAGO2 and HR components suggested a physical interaction between the HR and RNA silencing machinery in the process.

scholarly journals Bacillus Cyclic Lipopeptides Iturin and Fengycin Control Rice Blast Caused by Pyricularia oryzae in Potting and Acid Sulfate Soils by Direct Antagonism and Induced Systemic Resistance

2021 ◽  
Vol 9 (7) ◽  
pp. 1441
Author(s):  
Van Bach Lam ◽  
Thibault Meyer ◽  
Anthony Arguelles Arias ◽  
Marc Ongena ◽  
Feyisara Eyiwumi Oni ◽  
...  
Keyword(s):  
Rice Blast ◽  
Induced Systemic Resistance ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Systemic Resistance ◽  
Acid Sulfate Soils ◽  
Acid Sulfate ◽  
Wide Range ◽  
Bacillus Spp ◽  
Sulfate Soils

Rice monoculture in acid sulfate soils (ASSs) is affected by a wide range of abiotic and biotic constraints, including rice blast caused by Pyricularia oryzae. To progress towards a more sustainable agriculture, our research aimed to screen the biocontrol potential of indigenous Bacillus spp. against blast disease by triggering induced systemic resistance (ISR) via root application and direct antagonism. Strains belonging to the B. altitudinis and B. velezensis group could protect rice against blast disease by ISR. UPLC–MS and marker gene replacement methods were used to detect cyclic lipopeptide (CLiP) production and construct CLiPs deficient mutants of B. velezensis, respectively. Here we show that the CLiPs fengycin and iturin are both needed to elicit ISR against rice blast in potting soil and ASS conditions. The CLiPs surfactin, iturin and fengycin completely suppressed P. oryzae spore germination resulting in disease severity reduction when co-applied on rice leaves. In vitro microscopic assays revealed that iturin and fengycin inhibited the mycelial growth of the fungus P. oryzae, while surfactin had no effect. The capacity of indigenous Bacillus spp. to reduce rice blast by direct and indirect antagonism in ASS conditions provides an opportunity to explore their usage for rice blast control in the field.

Sign in / Sign up

Export Citation Format

Share Document