First Report of Charcoal Rot Caused by Macrophomina phaseolina on Brassica carinata in South Dakota

2015 ◽  
Vol 16 (3) ◽  
pp. 134-135
Author(s):  
C. Tande ◽  
A. Gebreil ◽  
F. Mathew ◽  
K. Grady ◽  
E. Byamukama
Keyword(s):  
South Dakota ◽  
Yield Loss ◽  
Macrophomina Phaseolina ◽  
Brassica Carinata ◽  
Charcoal Rot ◽  
First Report ◽  
Drought Conditions

This report indicates that Brassica carinata is a host for Macrophomina phaseolina and if susceptible crops (such as soybean and corn) are planted following B. carinata, presence of inoculum combined with drought conditions can favor charcoal rot development and cause yield loss. Accepted for publication 31 August 2015. Published 10 September 2015

scholarly journals First Report of Charcoal Rot in Tomato Caused by Macrophomina phaseolina (Tassi) Goid. From Pakistan

Plant Disease ◽  
2018 ◽  
Vol 102 (7) ◽  
pp. 1459 ◽  
Author(s):  
S. Hyder ◽  
A. S. Gondal ◽  
R. Ahmed ◽  
S. T. Sahi ◽  
A. Rehman ◽  
...  
Keyword(s):  
Macrophomina Phaseolina ◽  
Charcoal Rot ◽  
First Report

scholarly journals First report of charcoal rot caused by Macrophomina phaseolina on kiwifruit in Turkey

2019 ◽  
Vol 102 (2) ◽  
pp. 535-535
Author(s):  
Muharrem Türkkan ◽  
Halil İbrahim Benli ◽  
Özkan Yılmaz ◽  
Göksel Özer ◽  
Mehmet Yaman ◽  
...  
Keyword(s):  
Macrophomina Phaseolina ◽  
Charcoal Rot ◽  
First Report

scholarly journals First Report of Charcoal Rot of Sunflower in Minnesota, USA

2010 ◽  
Vol 11 (1) ◽  
pp. 48
Author(s):  
T. Gulya ◽  
A. Mengistu ◽  
K. Kinzer ◽  
N. Balbyshev ◽  
S. Markell
Keyword(s):  
United States ◽  
South Dakota ◽  
Great Plains ◽  
Northern Great Plains ◽  
Charcoal Rot ◽  
Midwestern United States ◽  
First Report ◽  
High Incidence ◽  
Sunflower Crop ◽  
North And South

Charcoal rot was first observed on sunflower in North and South Dakota in 1998, and was widespread on soybeans recently in Iowa, suggesting that Macrophomina may becoming more common in cooler growing areas of Midwestern United States. With the multitude of Macrophomina hosts in the northern Great Plains and the high incidence of microsclerotia we detected in soil, high disease potential may exist, suggesting that in drier, hotter years the sunflower crop may be affected by this disease. Accepted for publication 17 May 2010. Published 7 July 2010.

scholarly journals First Report of Charcoal Rot Caused by Macrophomina phaseolina on Hemp (Cannabis sativa) Varieties Cultivated in Southern Spain

Plant Disease ◽  
2018 ◽  
Vol 102 (8) ◽  
pp. 1665 ◽  
Author(s):  
S. Casano ◽  
A. Hernández Cotan ◽  
M. Marín Delgado ◽  
I. F. García-Tejero ◽  
O. Gómez Saavedra ◽  
...  
Keyword(s):  
Cannabis Sativa ◽  
Macrophomina Phaseolina ◽  
Southern Spain ◽  
Charcoal Rot ◽  
First Report

scholarly journals First Report of Charcoal Rot, Caused by Macrophomina phaseolina, on Blueberry in Southwestern Spain

Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2677-2677 ◽  
Author(s):  
B. de los Santos ◽  
A. Aguado ◽  
C. Borrero ◽  
J. Viejobueno ◽  
M. Avilés
Keyword(s):  
Macrophomina Phaseolina ◽  
Charcoal Rot ◽  
First Report

scholarly journals First Report of Charcoal Rot Caused by Macrophomina phaseolina in Basella alba in India

Plant Disease ◽  
2018 ◽  
Vol 102 (8) ◽  
pp. 1669 ◽  
Author(s):  
B. R. Meena ◽  
K. Nagendran ◽  
A. N. Tripathi ◽  
S. Kumari ◽  
V. Sagar ◽  
...  
Keyword(s):  
Macrophomina Phaseolina ◽  
Charcoal Rot ◽  
First Report ◽  
Basella Alba

scholarly journals First Report of Alternaria Black Spot caused by Alternaria alternata on Brassica carinata in South Dakota

Plant Disease ◽  
2017 ◽  
Vol 101 (11) ◽  
pp. 1951-1951 ◽  
Author(s):  
M. Dunbar ◽  
A. Adhikari ◽  
B. Kontz ◽  
A. Varenhorst ◽  
T. Nleya ◽  
...  
Keyword(s):  
South Dakota ◽  
Alternaria Alternata ◽  
Black Spot ◽  
Brassica Carinata ◽  
First Report

scholarly journals First Report of Charcoal Rot Caused by Macrophomina phaseolina on Sunflower in Illinois

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1318-1318 ◽  
Author(s):  
J. D. Weems ◽  
S. A. Ebelhar ◽  
V. Chapara ◽  
D. K. Pedersen ◽  
G. R. Zhang ◽  
...  
Keyword(s):  
Vascular Tissue ◽  
Pcr Amplification ◽  
Macrophomina Phaseolina ◽  
Lesion Length ◽  
Stem Tissue ◽  
Becton Dickinson ◽  
Charcoal Rot ◽  
University Of Illinois ◽  
First Report ◽  
Fayette County

In September 2009, sunflower (Helianthus annuus L.) plants (cv. Mycogen 8C451) from a University of Illinois field research trial in Fayette County, Illinois exhibited silvery gray girdling lesions on the lower stems and premature death. When lower stems and roots were split open, the pith tissue was compressed into layers. Black microsclerotia (90 to 180 μm) were present on the outside of the lower stem tissue and in the stem vascular tissue. Five pieces (approximately 1 cm long) of symptomatic stem tissue from five different affected plants (25 pieces total) were soaked in a 0.5% solution of NaOCl for 30 s, rinsed with sterile distilled water, and placed on potato dextrose agar (PDA; Becton, Dickinson, and Company, Franklin Lakes, NJ). Gray hyphae grew from all of the stem pieces, which subsequently turned black and formed black microsclerotia (75 to 175 μm). On the basis of plant symptoms and size and color of the microsclerotia, the disease was diagnosed as charcoal rot caused by Macrophomina phaseolina (Tassi) Goid (2). To confirm that the isolated fungus was M. phaseolina, DNA was extracted from the pure culture, and PCR amplification of a subunit rDNA and internal transcribed spacer (ITS) region with primers EF3RCNL and ITS4 was performed (3). The Keck Biotechnology Center at the University of Illinois, Urbana sequenced the PCR product. The resulting nucleotide sequence shared the highest homology (99%) with sequences of M. phaseolina when compared with the subunit rDNA and ITS sequences in the nucleotide database ( http://www.ncbi.nlm.nih.gov ). A greenhouse experiment was conducted to confirm pathogenicity; the greenhouse temperature was approximately 27°C and sunflower plants (cv. Cargill 270) were grown in pots and watered daily to maintain adequate soil moisture for growth. Sterile toothpicks were infested with M. phaseolina and placed through the stems (10 cm above the soil surface) of five 40-day-old sunflower plants that were approximately at growth stage R4 (1,4). Five sterile, noninfested toothpicks were similarly placed through sunflower plants to act as controls. Parafilm was used to hold the toothpick in the stem and seal the stem injury. Thirty-five days after inoculation, the mean lesion length on stems inoculated with M. phaseolina was 595 mm and no lesions developed on the control plants. M. phaseolina-inoculated plants also began to wilt and die. Cultures identical to the original M. phaseolina isolate were reisolated from stem lesions of the M. phaseolina-inoculated plants. This is the first report of charcoal rot on sunflower in Illinois to our knowledge. Sunflower is currently not a major crop grown in Illinois, but on-going research is focused on evaluating sunflower as a potential late-planted crop to follow winter wheat. If sunflower production increases in Illinois, growers may need to take precautions to manage charcoal rot. References: (1) L. K. Edmunds. Phytopathology 54:514, 1964. (2) T. Gulya et al. Page 263 in: Sunflower Technology and Production. American Society of Agronomy, Madison, WI, 1997. (3) N. S. Lord et al. FEMS Microbiol. Ecol. 42:327, 2002. (4) A. A. Schneiter and J. F. Miller. Crop Sci. 21:901, 1981.

scholarly journals First Report of Macrophomina phaseolina Causing a Crown Rot of Strawberry in Florida

Plant Disease ◽  
2005 ◽  
Vol 89 (4) ◽  
pp. 434-434 ◽  
Author(s):  
J. Mertely ◽  
T. Seijo ◽  
N. Peres
Keyword(s):  
Methyl Bromide ◽  
Block Design ◽  
Vascular Ring ◽  
Macrophomina Phaseolina ◽  
Crown Rot ◽  
American Phytopathological Society ◽  
Charcoal Rot ◽  
Isolation Medium ◽  
Potato Dextrose Broth ◽  
First Report

Strawberry (Fragaria × ananassa Duchesne) is produced as an annual winter crop in raised, plastic-mulched beds on 2,800 ha in west central Florida. In December 2001, a grower submitted collapsed and dying strawberry plants from a commercial field to the University of Florida in Dover. The cut crowns of affected plants revealed dark brown necrotic areas on the margins and along the woody vascular ring. Macrophomina phaseolina was isolated from pieces of infected tissue cut aseptically from the crowns and placed on a medium containing 12 g of Difco potato dextrose broth, 17 g of Bacto agar, 250 mg of ampicillin, and 100 mg of streptomycin sulfate per liter of water. The fungus produced numerous, dark, oblong sclerotia in the isolation medium after 4 to 5 days incubation at 24°C under constant fluorescent lighting. In 10-day-old cultures, sclerotia ranged in size from 55 to 190 μm long by 50 to 135 μm wide (average 105 × 74 μm). Ostiolate pycnidia bearing relatively large, broadly ellipsoidal, hyaline conidia occasionally developed on the host tissue after 8 to 10 days of incubation (2). During the 2003-2004 season, M. phaseolina was isolated from dying strawberry plants taken from the original field and two additional farms. Affected plants were often found along field margins or other areas inadequately fumigated with methyl bromide. Two single-spore isolates from different fields were tested for pathogenicity on nursery runner plants (cv. Strawberry Festival) grown for 4 weeks in the greenhouse on artificial potting soil. The fungal isolates were grown on corn meal agar at 24°C for 4 days and allowed to colonize sterile wooden toothpicks placed on the medium for an additional 5 days. Prior to use, the toothpicks were sterilized by autoclaving twice in deionized water and a third time in V8 juice. Six plants were inoculated with each isolate by inserting a colonized toothpick into each crown. Sterile, V8-infused toothpicks were inserted into the crowns of corresponding control plants. The plants were incubated in a greenhouse in a randomized complete block design with two replicates of three plants each. After 3 days, 33 to 100% of the inoculated plants developed wilting in one or more leaves. All inoculated plants collapsed or died within 2 weeks of inoculation, while the control plants remained healthy during the observation period. The pathogen was readily reisolated from inoculated plants. Charcoal rot disease caused by M. phaseolina has been reported on strawberry in France, India, and Illinois (2,3). To our knowledge, this is the first report from Florida. M. phaseolina may be an emerging threat as the Florida strawberry industry transitions from methyl bromide to other fumigants in 2005. References: (1) J. Maas. Macrophomina leaf blight and dry crown rot. Page 26 in: Compendium of Strawberry Diseases. 2nd ed. J. L. Maas, ed. The American Phytopathological Society, St. Paul, MN, 1998. (2) G. S. Smith and T. D. Wyllie. Charcoal rot. Pages 29–31 in: Compendium of Soybean Diseases. G. L. Hartman et al., eds. 4th ed. The American Phytopathological Society, St. Paul, MN. 1999. (3) B. Tweedy et al. Plant Dis. Rep. 42:107, 1958.

Sign in / Sign up

Export Citation Format

Share Document