Cotton Leaf Crumple Virus Transmitted from Naturally Infected Bean from Mexico

Plant Disease ◽  
1986 ◽  
Vol 70 (10) ◽  
pp. 981b ◽  
Author(s):  
J. K. Brown
Keyword(s):  
Cotton Leaf ◽  
Cotton Leaf Crumple Virus

Cotton Leaf Crumple Virus Disease in Okra-leaf and Normal-leaf Cottons

1985 ◽  
Vol 78 (6) ◽  
pp. 1500-1502 ◽  
Author(s):  
G. D. Butler ◽  
F. D. Wilson ◽  
T. J. Henneberry
Keyword(s):  
Virus Disease ◽  
Cotton Leaf ◽  
Normal Leaf ◽  
Cotton Leaf Crumple Virus

scholarly journals Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system

Plant Methods ◽  
2012 ◽  
Vol 8 (1) ◽  
pp. 27 ◽  
Author(s):  
John Tuttle ◽  
Candace H Haigler ◽  
Dominique Robertson
Keyword(s):  
Gene Silencing ◽  
Low Cost ◽  
Cotton Leaf ◽  
Virus Induced Gene Silencing ◽  
Cotton Leaf Crumple Virus

scholarly journals Cotton leaf crumple virus Is a Distinct Western Hemisphere Begomovirus Species with Complex Evolutionary Relationships Indicative of Recombination and Reassortment

2004 ◽  
Vol 94 (10) ◽  
pp. 1068-1074 ◽  
Author(s):  
A. M. Idris ◽  
J. K. Brown
Keyword(s):  
Sonoran Desert ◽  
Phylogenetic Analyses ◽  
Western Hemisphere ◽  
Bipartite Begomoviruses ◽  
Progeny Virus ◽  
Cotton Leaf ◽  
Sequence Comparisons ◽  
Squash Leaf Curl Virus ◽  
A Genome ◽  
Cotton Leaf Crumple Virus

The bipartite DNA genome of Cotton leaf crumple virus (CLCrV), a whitefly-transmitted begomovirus from the Sonoran Desert, was cloned and completely sequenced. The cloned CLCrV genome was infectious when biolistically delivered to cotton or bean seedlings and progeny virus was whitefly-transmissible. Koch's postulates were completed by the reproduction of characteristic leaf crumple symptoms in cotton seedlings infected with cloned CLCrV DNA, thereby verifying the etiology of leaf crumple disease, which has been known in the southwestern United States since the 1950s. Sequence comparisons confirmed that CLCrV has a genome organization typical of yet sufficiently divergent from all other bipartite begomoviruses to justify recognition as a distinct species. Phylogenetic analyses indicated that CLCrV has a complex evolutionary history probably involving both recombination and reassortment. The relatively low nucleotide sequence identity (77%) of the common region shared by the CLCrV DNA-A and DNA-B components and the distinct phylogenetic relationships of each component are consistent with component reassortment. Sequence analyses indicated that the CLCrV DNA-A component was likely derived by recombination among ancestors of two divergent clades (e.g., the Squash leaf curl virus [SLCV] clade and the Abutilon mosaic virus clade) of Western Hemisphere begomoviruses. The CLCrV DNA-B component also may have originated by recombination among an ancestor of the SLCV clade and another distantly related but unknown Western Hemisphere begomovirus.

scholarly journals Heritable gene editing using FT mobile guide RNAs and DNA viruses

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Jianfeng Lei ◽  
Peihong Dai ◽  
Yue Li ◽  
Wanqi Zhang ◽  
Guantong Zhou ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Apical Meristem ◽  
Gene Editing ◽  
Cotton Leaf ◽  
Virus Induced Gene Silencing ◽  
Flowering Locus T ◽  
Dna Viruses ◽  
Knock Out ◽  
Guide Rnas ◽  
Cotton Leaf Crumple Virus

Abstract Background The virus-induced genome editing (VIGE) system can be used to quickly identify gene functions and generate knock-out libraries as an alternative to the virus-induced gene silencing (VIGS). Although plant virus-mediated VIGE has been shown to have great application prospects, edited genes cannot be transferred to the next generations using this system, as viruses cannot enter into shoot apical meristem (SAM) in plants. Results We developed a novel cotton leaf crumple virus (CLCrV)-mediated VIGE system designed to target BRI1, GL2, PDS genes, and GUS transgene in A. thaliana by transforming Cas9 overexpression (Cas9-OE) A. thaliana. Given the deficiency of the VIGE system, ProYao::Cas9 and Pro35S::Cas9 A. thaliana were transformed by fusing 102 bp FT mRNAs with sgRNAs so as to explore the function of Flowering Locus T (FT) gene in delivering sgRNAs into SAM, thus avoiding tissue culture and stably acquiring heritable mutant offspring. Our results showed that sgRNAs fused with FT mRNA at the 5′ end (FT strategy) effectively enabled gene editing in infected plants and allowed the acquisition of mutations heritable by the next generation, with an efficiency of 4.35–8.79%. In addition, gene-edited offspring by FT-sgRNAs did not contain any components of the CLCrV genome. Conclusions FT strategy can be used to acquire heritable mutant offspring avoiding tissue culture and stable transformation based on the CLCrV-mediated VIGE system in A. thaliana.

Cotton leaf crumple virus. [Distribution map].

2005 ◽  
Author(s):  
Keyword(s):  
North America ◽  
Gossypium Hirsutum ◽  
Central America ◽  
Geographical Distribution ◽  
Cotton Leaf ◽  
Distribution Map ◽  
Gossypium Spp ◽  
Cotton Leaf Crumple Virus ◽  
Virus Distribution ◽  
New Distribution

Abstract A new distribution map is provided for Cotton leaf crumple virus Virus: Geminiviridae: Begomovirus Hosts: Cotton (Gossypium hirsutum and other Gossypium spp.), other Malvaceae, some records on Fabaceae (Glycine, Phaseolus, Vicia). Information is given on the geographical distribution in ASIA, India, Karnataka, Maharashtra, NORTH AMERICA, Mexico, USA, Arizona, California, Texas, CENTRAL AMERICA & CARIBBEAN, Guatemala.

scholarly journals Geminivirus-Mediated Gene Silencing from Cotton Leaf Crumple Virus Is Enhanced by Low Temperature in Cotton

2008 ◽  
Vol 148 (1) ◽  
pp. 41-50 ◽  
Author(s):  
John R. Tuttle ◽  
A.M. Idris ◽  
Judith K. Brown ◽  
Candace H. Haigler ◽  
Dominique Robertson
Keyword(s):  
Low Temperature ◽  
Gene Silencing ◽  
Cotton Leaf ◽  
Cotton Leaf Crumple Virus
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