Cotton Leaf Crumple Virus Disease in Okra-leaf and Normal-leaf Cottons

1985 ◽  
Vol 78 (6) ◽  
pp. 1500-1502 ◽  
Author(s):  
G. D. Butler ◽  
F. D. Wilson ◽  
T. J. Henneberry
Keyword(s):  
Virus Disease ◽  
Cotton Leaf ◽  
Normal Leaf ◽  
Cotton Leaf Crumple Virus

scholarly journals Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system

Plant Methods ◽  
2012 ◽  
Vol 8 (1) ◽  
pp. 27 ◽  
Author(s):  
John Tuttle ◽  
Candace H Haigler ◽  
Dominique Robertson
Keyword(s):  
Gene Silencing ◽  
Low Cost ◽  
Cotton Leaf ◽  
Virus Induced Gene Silencing ◽  
Cotton Leaf Crumple Virus

Cotton Leaf Curl Virus Disease

1970 ◽  
Vol 16 (1) ◽  
pp. 121-131
Author(s):  
E. El Nur ◽  
H. S. Abu Salih
Keyword(s):  
Virus Disease ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

scholarly journals Cotton leaf crumple virus Is a Distinct Western Hemisphere Begomovirus Species with Complex Evolutionary Relationships Indicative of Recombination and Reassortment

2004 ◽  
Vol 94 (10) ◽  
pp. 1068-1074 ◽  
Author(s):  
A. M. Idris ◽  
J. K. Brown
Keyword(s):  
Sonoran Desert ◽  
Phylogenetic Analyses ◽  
Western Hemisphere ◽  
Bipartite Begomoviruses ◽  
Progeny Virus ◽  
Cotton Leaf ◽  
Sequence Comparisons ◽  
Squash Leaf Curl Virus ◽  
A Genome ◽  
Cotton Leaf Crumple Virus

The bipartite DNA genome of Cotton leaf crumple virus (CLCrV), a whitefly-transmitted begomovirus from the Sonoran Desert, was cloned and completely sequenced. The cloned CLCrV genome was infectious when biolistically delivered to cotton or bean seedlings and progeny virus was whitefly-transmissible. Koch's postulates were completed by the reproduction of characteristic leaf crumple symptoms in cotton seedlings infected with cloned CLCrV DNA, thereby verifying the etiology of leaf crumple disease, which has been known in the southwestern United States since the 1950s. Sequence comparisons confirmed that CLCrV has a genome organization typical of yet sufficiently divergent from all other bipartite begomoviruses to justify recognition as a distinct species. Phylogenetic analyses indicated that CLCrV has a complex evolutionary history probably involving both recombination and reassortment. The relatively low nucleotide sequence identity (77%) of the common region shared by the CLCrV DNA-A and DNA-B components and the distinct phylogenetic relationships of each component are consistent with component reassortment. Sequence analyses indicated that the CLCrV DNA-A component was likely derived by recombination among ancestors of two divergent clades (e.g., the Squash leaf curl virus [SLCV] clade and the Abutilon mosaic virus clade) of Western Hemisphere begomoviruses. The CLCrV DNA-B component also may have originated by recombination among an ancestor of the SLCV clade and another distantly related but unknown Western Hemisphere begomovirus.

scholarly journals Heritable gene editing using FT mobile guide RNAs and DNA viruses

Plant Methods ◽  
2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Jianfeng Lei ◽  
Peihong Dai ◽  
Yue Li ◽  
Wanqi Zhang ◽  
Guantong Zhou ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Apical Meristem ◽  
Gene Editing ◽  
Cotton Leaf ◽  
Virus Induced Gene Silencing ◽  
Flowering Locus T ◽  
Dna Viruses ◽  
Knock Out ◽  
Guide Rnas ◽  
Cotton Leaf Crumple Virus

Abstract Background The virus-induced genome editing (VIGE) system can be used to quickly identify gene functions and generate knock-out libraries as an alternative to the virus-induced gene silencing (VIGS). Although plant virus-mediated VIGE has been shown to have great application prospects, edited genes cannot be transferred to the next generations using this system, as viruses cannot enter into shoot apical meristem (SAM) in plants. Results We developed a novel cotton leaf crumple virus (CLCrV)-mediated VIGE system designed to target BRI1, GL2, PDS genes, and GUS transgene in A. thaliana by transforming Cas9 overexpression (Cas9-OE) A. thaliana. Given the deficiency of the VIGE system, ProYao::Cas9 and Pro35S::Cas9 A. thaliana were transformed by fusing 102 bp FT mRNAs with sgRNAs so as to explore the function of Flowering Locus T (FT) gene in delivering sgRNAs into SAM, thus avoiding tissue culture and stably acquiring heritable mutant offspring. Our results showed that sgRNAs fused with FT mRNA at the 5′ end (FT strategy) effectively enabled gene editing in infected plants and allowed the acquisition of mutations heritable by the next generation, with an efficiency of 4.35–8.79%. In addition, gene-edited offspring by FT-sgRNAs did not contain any components of the CLCrV genome. Conclusions FT strategy can be used to acquire heritable mutant offspring avoiding tissue culture and stable transformation based on the CLCrV-mediated VIGE system in A. thaliana.

Cotton leaf curl virus disease

2000 ◽  
Vol 71 (1-2) ◽  
pp. 151-159 ◽  
Author(s):  
R.W Briddon ◽  
P.G Markham
Keyword(s):  
Virus Disease ◽  
Cotton Leaf ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Leaf Curl

Effect of Cotton Leaf Curl Virus Disease on Morphology, Yield and Fibre Characteristics of Susceptible Lines/cultivars of Cotton (Gossypium hirsutum L.)

2002 ◽  
Vol 1 (6) ◽  
pp. 705-707 ◽  
Author(s):  
Ghayour Ahmad ◽  
Saeed Ahmad Malik . ◽  
Zahid Mamood . ◽  
Muhammad Zaffar Iqba . ◽  
Saghir Ahmad . ◽  
...  
Keyword(s):  
Gossypium Hirsutum ◽  
Virus Disease ◽  
Cotton Leaf ◽  
Gossypium Hirsutum L ◽  
Cotton Leaf Curl Virus ◽  
Leaf Curl Virus ◽  
Fibre Characteristics ◽  
Leaf Curl
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