Virus-Free Shoots from Cassava Stem Cuttings Infected with Cassava Latent Virus

Plant Disease ◽  
1985 ◽  
Vol 69 (3) ◽  
pp. 231 ◽  
Author(s):  
R. P. Pacumbaba
Keyword(s):  
Latent Virus ◽  
Stem Cuttings ◽  
Cassava Stem ◽  
Cassava Latent Virus

scholarly journals Development of a triple antibody sandwich enzyme-linked immunosorbent assay for cassava mosaic disease detection using a monoclonal antibody to Sri Lankan cassava mosaic virus

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Saengsoon Charoenvilaisiri ◽  
Channarong Seepiban ◽  
Mallika Kumpoosiri ◽  
Sombat Rukpratanporn ◽  
Nuchnard Warin ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Immunosorbent Assay ◽  
Mosaic Disease ◽  
Cassava Mosaic Disease ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sri Lankan ◽  
Stem Cuttings ◽  
Field Samples ◽  
Cassava Stem ◽  
Cassava Mosaic Virus

Abstract Background Cassava mosaic disease (CMD) is one of the most devastating viral diseases for cassava production in Africa and Asia. Accurate yet affordable diagnostics are one of the fundamental tools supporting successful CMD management, especially in developing countries. This study aimed to develop an antibody-based immunoassay for the detection of Sri Lankan cassava mosaic virus (SLCMV), the only cassava mosaic begomovirus currently causing CMD outbreaks in Southeast Asia (SEA). Methods Monoclonal antibodies (MAbs) against the recombinant coat protein of SLCMV were generated using hybridoma technology. MAbs were characterized and used to develop a triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA) for SLCMV detection in cassava leaves and stems. Assay specificity, sensitivity and efficiency for SLCMV detection was investigated and compared to those of a commercial ELISA test kit and PCR, the gold standard. Results A TAS-ELISA for SLCMV detection was successfully developed using the newly established MAb 29B3 and an in-house polyclonal antibody (PAb) against begomoviruses, PAb PK. The assay was able to detect SLCMV in leaves, green bark from cassava stem tips, and young leaf sprouts from stem cuttings of SLCMV-infected cassava plants without cross-reactivity to those derived from healthy cassava controls. Sensitivity comparison using serial dilutions of SLCMV-infected cassava sap extracts revealed that the assay was 256-fold more sensitive than a commercial TAS-ELISA kit and 64-fold less sensitive than PCR using previously published SLCMV-specific primers. In terms of DNA content, our assay demonstrated a limit of detection of 2.21 to 4.08 × 106 virus copies as determined by quantitative real-time PCR (qPCR). When applied to field samples (n = 490), the TAS-ELISA showed high accuracy (99.6%), specificity (100%), and sensitivity (98.2%) relative to the results obtained by the reference PCR. SLCMV infecting chaya (Cnidoscolus aconitifolius) and coral plant (Jatropha multifida) was also reported for the first time in SEA. Conclusions Our findings suggest that the TAS-ELISA for SLCMV detection developed in this study can serve as an attractive tool for efficient, inexpensive and high-throughput detection of SLCMV and can be applied to CMD screening of cassava stem cuttings, large-scale surveillance, and screening for resistance.

Nucleotide sequence of cassava latent virus DNA

Nature ◽  
1983 ◽  
Vol 301 (5897) ◽  
pp. 260-262 ◽  
Author(s):  
John Stanley ◽  
Michael R. Gay
Keyword(s):  
Nucleotide Sequence ◽  
Latent Virus ◽  
Cassava Latent Virus

SIMULATION OF THE DYNAMICS OF CASSAVA STEM CUTTINGS ON AN INCLINED WOOD SURFACE

2018 ◽  
Vol 14 (2) ◽  
pp. 85-97
Author(s):  
I A OLA ◽  
O O AREMU ◽  
A A ADERINLEWO ◽  
E S.A AJISEGIRI ◽  
P O AIYEDUN ◽  
...  
Keyword(s):  
Wood Surface ◽  
Great Influence ◽  
Stem Cuttings ◽  
Flow Properties ◽  
Angle Variation ◽  
Predictive Parameters ◽  
Major Transition ◽  
Cassava Stem ◽  
The Tropics ◽  
Precision Machines

Development of precision machines to handle some of the field activities involved in the cultivation of cassava is a highly welcomed project among young enterprising farmers who are keen in resolving the posterity of their wellbeing as a big time farmer. Mechanization of cassava in the tropics is grossly hampered by lack of extensive knowlegde on the behaviour, physical properties of cassava stems and parameters that have great influence on the handling of the stems during cultivation, hence the limita-tion of advancing the present semi-automatic planter to fully automated one is still a mirage in the tropics. This paper presents the flow properties of cassava cuttings that can lend it to proper handling in a metering machine. A variable inclined wood surface rig with an ejection outlet was used to study the properties of the stem. Measured cassava stem predictive parameters; diamater, lentgh,weight; angle variation on the rig; response parameters; percentage ejected, percentage sliding through, forces acting on stems were obtained. The results from the study could be used to predict acurately the exact dynamics of a given stem isolated from the bulk of stem cuttings stacked in the metering hopper. Models to predict the flow properties of the stems were obtained; further simulation of the dynamics was embarked upon using MATLAB software. A major transition zone of stem characteris-tics per pecentage sliding to ejection occurred within the range of angles of 150 -300. This might be a favourable angular zone for isolating the stems from the bulk mass while the effectively isolated stems can be metered appropriately by another mechanism in the process of developing a metering device for cassava stem cuttings.

Cassava latent virus infections mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric viral DNA

1988 ◽  
Vol 11 (6) ◽  
pp. 795-803 ◽  
Author(s):  
Bret A. M. Morris ◽  
Kim A. Richardson ◽  
Mark T. Andersen ◽  
Richard C. Gardner
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Ti Plasmid ◽  
Latent Virus ◽  
Virus Infections ◽  
Viral Dna ◽  
Cassava Latent Virus

scholarly journals Pseudorecombinants between Cloned DNAs of Two Isolates of Cassava Latent Virus

1985 ◽  
Vol 66 (5) ◽  
pp. 1055-1061 ◽  
Author(s):  
J. Stanley ◽  
R. Townsend ◽  
S. J. Curson
Keyword(s):  
Latent Virus ◽  
Cassava Latent Virus

scholarly journals Delimitation of essential genes of cassava latent virus DNA 2

1988 ◽  
Vol 16 (11) ◽  
pp. 4811-4829 ◽  
Author(s):  
Pantea Etessami ◽  
Rowena Callis ◽  
Susan Ellwood ◽  
John Stanley
Keyword(s):  
Essential Genes ◽  
Latent Virus ◽  
Dna 2 ◽  
Cassava Latent Virus
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