Cassava latent virus infections mediated by the Ti plasmid of Agrobacterium tumefaciens containing either monomeric or dimeric viral DNA

1988 ◽  
Vol 11 (6) ◽  
pp. 795-803 ◽  
Author(s):  
Bret A. M. Morris ◽  
Kim A. Richardson ◽  
Mark T. Andersen ◽  
Richard C. Gardner
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Ti Plasmid ◽  
Latent Virus ◽  
Virus Infections ◽  
Viral Dna ◽  
Cassava Latent Virus

scholarly journals Correction: characterization of the virB operon from Agrobacterium tumefaciens Ti plasmid.

1990 ◽  
Vol 265 (8) ◽  
pp. 4768
Author(s):  
J E Ward ◽  
D E Akiyoshi ◽  
D Regier ◽  
A Datta ◽  
M P Gordon ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Ti Plasmid ◽  
Virb Operon

Nucleotide sequence of cassava latent virus DNA

Nature ◽  
1983 ◽  
Vol 301 (5897) ◽  
pp. 260-262 ◽  
Author(s):  
John Stanley ◽  
Michael R. Gay
Keyword(s):  
Nucleotide Sequence ◽  
Latent Virus ◽  
Cassava Latent Virus

Physical mapping of DNA base sequence homologies between an octopine and a nopaline Ti plasmid of Agrobacterium tumefaciens

1981 ◽  
Vol 152 (2) ◽  
pp. 183-208 ◽  
Author(s):  
G. Engler ◽  
A. Depicker ◽  
R. Maenhaut ◽  
R. Villarroel ◽  
M. Van Montagu ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Physical Mapping ◽  
Ti Plasmid ◽  
Base Sequence ◽  
Dna Base ◽  
Sequence Homologies

scholarly journals Mutational analysis of the virulence region of an Agrobacterium tumefaciens Ti plasmid.

1983 ◽  
Vol 153 (2) ◽  
pp. 878-883 ◽  
Author(s):  
H J Klee ◽  
F F White ◽  
V N Iyer ◽  
M P Gordon ◽  
E W Nester
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Mutational Analysis ◽  
Ti Plasmid

scholarly journals A novel plasmid-transcribed regulatory sRNA, QfsR, controls chromosomal polycistronic mRNAs in Agrobacterium tumefaciens

2019 ◽  
Author(s):  
Benjamin Diel ◽  
Magali Dequivre ◽  
Florence Wisniewski-Dyé ◽  
Ludovic Vial ◽  
Florence Hommais
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Small Rna ◽  
Regulatory Networks ◽  
Target Prediction ◽  
Ti Plasmid ◽  
Conjugative Transfer ◽  
Polycistronic Mrna ◽  
Cell Functions ◽  
Coordinated Expression ◽  
Cellular Behaviour

AbstractPlasmids are mobile DNAs that adjust host cell functions for their own amplification and dissemination. We identified QfsR, a small RNA, transcribed from the Ti plasmid in the phytopathogen Agrobacterium fabrum. QfsR is widely conserved throughout RepABC plasmids carried by Rhizobiaceae. Target prediction, expression analysis and site-direct mutagenesis experiments show that QfsR directly pairs within polycistronic mRNAs transcribed from chromosomes (involved in flagella apparatus and succinoglycan biosynthesis) and Ti plasmid (involved in conjugative transfer). QfsR leads to a coordinated expression of whole polycistronic mRNA molecules. Whereas a lack of QfsR induces motility and reduces succinoglycan production, its overproduction increases the quorum sensing signal accumulation and the Ti plasmid conjugative transfer. Based on these observations, we propose QfsR as a hub connecting regulatory networks of motility, succinoglycan biosynthesis and plasmid conjugative transfer. To our knowledge, QfsR is the first example of a plasmid-encoded sRNA that controls chromosomal polycistronic mRNAs.SignificancePlasmids represent an important cost for the hosting cell although some are beneficial under certain circumstances. Agrobacterium tumefaciens harboring Tumor inducing plasmid (pTi) are able to infect plants and to use specific resources produced by the infected cells. We characterized QfsR, a novel small RNA (sRNA) from pTi, that directly regulates plasmid polycistronic mRNA but also chromosomal ones. QfsR contributes to a fine-tuned regulation of bacterial motility, exopolysaccharide biosynthesis and conjugative dissemination of pTi. Our results report the first plasmid-encoded sRNA able to modify and coordinate cellular behaviour probably for the benefit of the plasmid dissemination and tight crosstalk between plasmid and chromosome. This could be widespread since QfsR homologs were predicted in other plasmids of Rhizobiaceae symbionts and pathogens.

Sign in / Sign up

Export Citation Format

Share Document