Expression of Extracellular Glycoproteins in the Uninfected Cells of Developing Pea Nodule Tissue

1991 ◽  
Vol 4 (6) ◽  
pp. 563 ◽  
Author(s):  
A. L. Rae
Keyword(s):  
Extracellular Glycoproteins ◽  
Nodule Tissue

NITROGENASE ACTIVITY OF ALFALFA GROWN ALONE AND IN MIXTURE WITH GRASS UNDER GREENHOUSE CONDITIONS

1985 ◽  
Vol 65 (3) ◽  
pp. 787-791
Author(s):  
H. A. BURITY ◽  
B. E. COULMAN ◽  
M. A. FARIS
Keyword(s):  
Medicago Sativa ◽  
Nitrogenase Activity ◽  
Dactylis Glomerata ◽  
Phleum Pratense ◽  
Bromus Inermis ◽  
Smooth Bromegrass ◽  
Medicago Sativa L ◽  
Dactylis Glomerata L ◽  
Phleum Pratense L ◽  
Nodule Tissue

A greenhouse experiment has shown that total nitrogenase activity of alfalfa (Medicago sativa L.) is not significantly affected when grown in association with timothy (Phleum pratense L.), smooth bromegrass (Bromus inermis Leyss) or orchardgrass (Dactylis glomerata L.) except after initial harvest when decreased alfalfa activity was associated with smooth bromegrass or orchardgrass. It was concluded that mixed cultures of alfalfa with timothy, smooth bromegrass or orchardgrass have no effect on alfalfa N2 fixation. The results also suggest the occurrence of N transference from alfalfa to associated grasses. It is speculated that this transfer is not primarily due to the death of roots and nodule tissue (after harvest), but involves some degree of N excretion during the period before initial harvest.Key words: Alfalfa-grass mixtures, N2-fixation, nodule activity, N-transference

scholarly journals Bacteriohopanetetrol: Abundant Lipid in Frankia Cells and in Nitrogen-Fixing Nodule Tissue

1991 ◽  
Vol 95 (1) ◽  
pp. 111-115 ◽  
Author(s):  
Alison M. Berry ◽  
Robert A. Moreau ◽  
A. Daniel Jones
Keyword(s):  
Nitrogen Fixing ◽  
Nodule Tissue

Extracellular glycoproteins at acetylcholine receptor clusters of rat myotubes are organized into domains

1990 ◽  
Vol 189 (1) ◽  
pp. 41-50 ◽  
Author(s):  
George M. Dmytrenko ◽  
Malka G. Scher ◽  
Giancarlo Poiana ◽  
Manfred Baetscher ◽  
Robert J. Bloch
Keyword(s):  
Acetylcholine Receptor ◽  
Receptor Clusters ◽  
Extracellular Glycoproteins

Soybean root response to infection by Rhizobium japonicum: saccharidases in root and nodule tissue

1984 ◽  
Vol 24 (1) ◽  
pp. 9-16 ◽  
Author(s):  
S. Bassarab ◽  
W. Dittrich ◽  
R.B. Mellor ◽  
D. Werner
Keyword(s):  
Rhizobium Japonicum ◽  
Soybean Root ◽  
Root Response ◽  
Nodule Tissue

scholarly journals Physical Environment and Symbiotic Nitrogen Fixation VI. Nitrogen Retention Within the Nodules of Trifolium Subterraneum L

1969 ◽  
Vol 22 (4) ◽  
pp. 829 ◽  
Author(s):  
AH Gibson
Keyword(s):  
Nitrogen Fixation ◽  
Physical Environment ◽  
Effective Strain ◽  
Nitrogen Retention ◽  
Trifolium Subterraneum ◽  
Dry Weight ◽  
Root Temperature ◽  
Protein Nitrogen ◽  
The Difference ◽  
Nodule Tissue

The effect of bacterial strain and root temperature on the retention of nitrogen in the root system of Trifolium Bubterraneum plants was re-examined. The root systems of plants nodulated by the moderately effective Rhizobium trifolii strain NA30 possessed a higher percentage nitrogen than those nodulated by the fully effective strain TAl, although the number of nodules formed by each strain was similar. The difference was due to a greater weight of nodule tissue on the NA30-nodulated plants, and also to a higher percentage nitrogen in the NA30 nodules; this latter effect was due to a higher concentration of non-protein nitrogen. The overall effect of these differences was to reduce the amount of nitrogen translocated to the shoots of the NA30 plants, in both absolute terms and as a proportion of the total amount of nitrogen fixed. Another difference between the two strains was the rate of nitrogen fixation per unit (dry weight or leghaemoglobin content) of nodule tissue.

Use of antibody and lectin-colloidal gold conjugates to detect surface and extracellular glycoproteins produced in vivo by the Lyme disease spirochete Borrelia bergdorferi

1990 ◽  
Vol 48 (3) ◽  
pp. 934-935
Author(s):  
David W. Dorward ◽  
Claude F. Garon
Keyword(s):  
Electron Microscopy ◽  
Lyme Disease ◽  
Colloidal Gold ◽  
Clinical Symptoms ◽  
Protein A ◽  
Rabbit Serum ◽  
Extracellular Material ◽  
Electron Microscopic ◽  
Extracellular Glycoproteins

Despite the often serious pathological ramifications of Lyme disease, Borrelia burgdorferi, the causative agent, is rarely demonstrated in nor isolated from naturally-infected mammalian hosts. This dilemma has led to suggestions that a limited number of spirochetes expel extracellular bioproducts that may contribute to the clinical symptoms. Currently, no mammalian toxins have been attributed to B. burgdorferi, however, the production of extracellular vesicles by this bacterium was recently described. In order to assay the possible presence of such extracellular material in infected hosts, we developed a sensitive electron microscopic assay to detect and characterize B. burgdorferi products in biological samples.Polyclonal rabbit serum was raised against purified B. burgdorferi vesicles and against an electrophoretically-purified 83 kilodalton (kDa) vesicle-associated protein. After purification of IgG from the sera, anti-vesicle F(ab’)2 fragments were produced and used to “activate” parlodion-coated grids. Such grids were incubated with cultured spirochetes, or with fluids and tissues from infected and non-infected mammals and ticks. Captured antigens were then labeled with anti-83 kDa IgG and Protein A-colloidal gold conjugates, and/or lectin-colloidal gold conjugates, then examined and characterized by electron microscopy.

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