scholarly journals Mothers ‘don’t like it; never tried it': Blind Sensory Test of a Homemade Chicken Liver Baby Food, a Source of Iron, by Infants and their Mothers

2015 ◽  
Vol 61 (4) ◽  
pp. 279-285
Author(s):  
Viviani Jaques Campos ◽  
Tania Beninga Morais
Keyword(s):  
Author(s):  
Soichiro Arai ◽  
Yuh H. Nakanishi

Although many electron microscopic studies on extracted chromatin have provided considerable information on chromatin condensation induced by divalent cations, there is only a little literature available on the effects of divalent cations on chromatin structure in intact nuclei. In the present study, the effects of Mg2+ on chromatin structure in isolated chicken liver nuclei were examined over a wide concentration range of Mg2+ by scanning electron microscopy.Nuclei were prepared from chicken liver by the method of Chauveau et al. with some modifications. The nuclei were suspended in 25 mM triethanolamine chloride buffer (pH7.4) with 1 mM EDTA or in the buffer with concentrations of MgCl2 varying from 1 to 50 mM. After incubation for 1 min at 0°C, glutaraldehyde was added to 1.8% and the nuclei were fixed for 1 h at 4°C. The fixed nuclei were mixed with 15% gelatin solution warmed at about 40°C, and kept at room temperature until the mixture set. The gelatin containing the nuclei was fixed with 2% glutaraldehyde for 2-4 h, and cut into small blocks. The gelatin blocks were conductive-stained with 2% tannic acid and 2% osmium tetroxide, dehydrated in a graded series of ethanol, and freeze-cracked with a razor blade in liquid nitrogen.


1970 ◽  
Author(s):  
Kenneth B. Stein ◽  
Peter Lenrow
Keyword(s):  

2008 ◽  
Vol 60 (1) ◽  
pp. 75-89 ◽  
Author(s):  
Mady A. Ismail ◽  
Hannington K. Taligoola ◽  
Rebbecca Nakamya

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