scholarly journals Evaluation of the Newly Developed Immunochromatography Test Kit for Rapid Detection and Differentiation of Norovirus GI and GII

2010 ◽  
Vol 56 (5) ◽  
pp. 368-369 ◽  
Author(s):  
P. Khamrin ◽  
W. Chan-it ◽  
K. Satou ◽  
Y. Nanba ◽  
Y. Yamashita ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Test Kit

scholarly journals Comparative Sensitivity of Rapid Antigen Tests for the Delta Variant (B.1.617.2) of SARS-CoV-2

Viruses ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2183
Author(s):  
Yuko Sakai-Tagawa ◽  
Seiya Yamayoshi ◽  
Peter J. Halfmann ◽  
Yoshihiro Kawaoka
Keyword(s):  
Rapid Detection ◽  
Influenza A ◽  
Infectious Virus ◽  
Rapid Test ◽  
Rapid Diagnosis ◽  
Antigen Test ◽  
Detection Level ◽  
Test Kit ◽  
Low Levels ◽  
Antigen Tests

Rapid antigen tests (RATs) for COVID-19 based on lateral flow immunoassays are useful for rapid diagnosis in a variety of settings. Although many kinds of RATs are available, their respective sensitivity has not been compared. Here, we examined the sensitivity of 27 RATs available in Japan for the detection of the SARS-CoV-2 delta variant. All of the RATs tested detected the delta variant albeit with different sensitivities. Nine RATs (ESPLINE SARS-CoV-2, ALSONIC COVID-19 Ag, COVID-19 and Influenza A+B Antigen Combo Rapid Test, ImmunoArrow SARS-CoV-2, Fuji Dri-chem immuno AG cartridge COVID-19 Ag, 2019-nCoV Ag rapid detection kit, Saliva SARS-CoV-2(2019-nCoV) Antigen Test Kit, and Rabliss SARS-CoV-2 antigen detection kit COVID19 AG) showed superior sensitivity to the isolated delta variant. Although actual clinical specimens were not examined, the detection level of most of the RATs was 7500 pfu, indicating that individuals whose test samples contained less virus than that would be considered negative. Therefore, it is important to bear in mind that RATs may miss individuals shedding low levels of infectious virus.

scholarly journals The rapid detection of E. coli 0157 Antigen in meat products by using ELISA Test Kit

2011 ◽  
Vol 35 (2) ◽  
pp. 61-65
Author(s):  
Maithem Ihsan Abdulrasool
Keyword(s):  
Rapid Detection ◽  
Meat Products ◽  
Storage Conditions ◽  
Elisa Test ◽  
E Coli ◽  
Elisa Kit ◽  
Beef Meat ◽  
Consumer Safety ◽  
Test Kit ◽  
Beef Burger

This study was conducted for rapid detection of contamination of meat products with E. coli 0157 by usage of ELISA test kit as one of the most rapid and newest test. The study showed the efficiency of ELISA test kit used in this study in detection of bacteria antigen in meat products samples of raw beef meat , kabab and beef burger which subjected to improper storage conditions or undercooked and hence when some peoples uptake some of these products they suffered from clinical intoxication signs like diarrhea ,vomiting and hyperthermia. The kit showed the presence of (9) samples positive among (90) samples ; (2) positive out of (30) kabab samples,(3) positive out of (30) beef burger and (4) positive out of (30) raw beef meat after 16 hrs of enrichment of all samples in EC Modified broth including Novobiocin supplement as inhibitor of other bacteria and then the liquid supernatants from all prepared samples got tested by ELISA kit used in this study and the data recovered in less than 1 hr.The study indicated the ability of using ELISA kit for detection of E. coli 0157 antigens in food stuffs and reduce the time for releasing the results in less than 24 hrs when compared with conventional culturing procedure which reuiqred more than 3 days and launch the food products for consumption with focusing on the main point here which is the protection of our consumer safety.

Evaluation of the Verigene Warfarin Metabolism Nucleic Acid Test Kit for the Rapid Detection ofCYP2C9andVKORC1Polymorphisms

2014 ◽  
Vol 4 (4) ◽  
pp. 198 ◽  
Author(s):  
Hyeyoung Lee ◽  
Jiyeon Kim ◽  
Gun Dong Lee ◽  
Joonhong Park ◽  
Hyojin Chae ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rapid Detection ◽  
Nucleic Acid Test ◽  
Test Kit ◽  
Acid Test

scholarly journals Evaluation of the Rapidec Carba NP Test Kit for Detection of Carbapenemase-Producing Gram-Negative Bacteria

2015 ◽  
Vol 59 (12) ◽  
pp. 7870-7872 ◽  
Author(s):  
Atul Garg ◽  
Jaya Garg ◽  
G. C. Upadhyay ◽  
Anurag Agarwal ◽  
Amitabha Bhattacharjee
Keyword(s):  
Early Detection ◽  
Rapid Detection ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Predictive Values ◽  
Gram Negative ◽  
Practical Solution ◽  
Test Kit ◽  
Sensitivity Specificity

ABSTRACTRecently, bioMérieux, France, introduced the Rapidec Carba NP test kit for rapid detection of carbapenemase-producing Gram-negative bacteria. This kit was evaluated in this study, and we report sensitivity, specificity, and positive and negative predictive values of 92.6%, 96.2%, 95.83%, and 92.6%, respectively. The test was easy to perform and interpret and relatively inexpensive ($5/Rs 300 per test) and provides a practical solution for early detection of carbapenemase-producing, multidrug-resistant Gram-negative bacteria.

Comparison of the Difco EZ Coli™ Rapid Detection System and Petrifilm™ Test Kit-HEC for Detection of Escherichia coli O157:H7 in Fresh and Frozen Ground Beef

1998 ◽  
Vol 61 (1) ◽  
pp. 110-112 ◽  
Author(s):  
JON-MIKEL WOODY ◽  
JOHN A. STEVENSON ◽  
RICHARD A. WILSON ◽  
STEPHEN J. KNABEL
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Detection System ◽  
Screening Method ◽  
Frozen Storage ◽  
Ground Beef ◽  
Escherichia Coli O157 ◽  
Enrichment Broth ◽  
E Coli ◽  
Test Kit

The Difco EZ Coli™ Rapid Detection System was compared to the 3M Petrifilm™ method for detection of Escherichia coli O157:H7 in raw ground beef. Raw meatballs (25 g) were inoculated with 10 to 15 cells of Escherichia coli O157:H7, stored for various times and at different temperatures, and then stomached for 2 min in 225 ml of EZ Coli™ enrichment broth, which was then incubated at 42°C for 18 to 24 h. A 1-ml sample of the enrichment broth was loaded into the top of the detector tips and the remaining EZ Coli™ broth held at 35°C before streaking onto MacConkey sorbitol agar and tryptic soy agar with yeast extract. A duplicate set of meatballs were tested using the 3M Petrifilm™ Test Kit-HEC for hemorrhagic Escherichia coli O157:H7. In this method raw meatballs (25 g) were enriched for 6 h in modified EC broth containing novobiocin at 37°C prior to inoculation of the Petrifilm™ E. coli Count Plates, which were incubated at 42°C for 18 h. The immunoblot ELISA was performed following this incubation. Presumptive positive isolates from both methods were confirmed using Oxoid E. coli Latex Agglutination and Difco Pasco ID Tripanels. Both methods permitted detection of 10 to 15 cells of E. coli O157:H7 per ml (i) immediately following inoculation, (ii) after 3 days of refrigerated storage at 8°C, and (iii) after 30 days in frozen storage at −20°C. The Difco EZ Coli™ Detection System proved to be a simpler and faster screening method with identification of negative and presumptive positive samples within 15 to 18 h

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