Evaluation of a commercial test kit for rapid detection ofStaphylococcus aureus in blood cultures

1997 ◽  
Vol 16 (2) ◽  
pp. 165-166 ◽  
Author(s):  
C. Neyret ◽  
H. Lelièvre ◽  
G. Lina ◽  
F. Vandenesch ◽  
J. Etienne
Keyword(s):  
Rapid Detection ◽  
Blood Cultures ◽  
Ofstaphylococcus Aureus ◽  
Commercial Test ◽  
Test Kit

scholarly journals Controlled Multicenter Evaluation of a Bacteriophage-Based Method for Rapid Detection of Staphylococcus aureus in Positive Blood Cultures

2013 ◽  
Vol 51 (4) ◽  
pp. 1226-1230 ◽  
Author(s):  
T. Bhowmick ◽  
S. Mirrett ◽  
L. B. Reller ◽  
C. Price ◽  
C. Qi ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Rapid Detection ◽  
Blood Cultures

Detection ofStaphylococcus aureus in peripheral blood stem cell cultures after sterilization of standard blood cultures

2003 ◽  
Vol 18 (1) ◽  
pp. 37-39
Author(s):  
Mitchell J. Schwaber ◽  
Carolyn N. Krasner ◽  
Howard S. Gold ◽  
Lata Venkataraman ◽  
David E. Avigan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Cultures ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cell ◽  
Blood Cultures ◽  
Blood Stem Cell ◽  
Ofstaphylococcus Aureus

scholarly journals Rapid Detection of PBP2a in Staphylococci from Shortly Incubated Subcultures of Positive Blood Cultures by an Immunochromatographic Assay

2021 ◽  
Author(s):  
Natalia Kolesnik-Goldmann ◽  
Elias Bodendoerfer ◽  
Kim Röthlin ◽  
Sebastian Herren ◽  
Frank Imkamp ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Patient Care ◽  
Rapid Detection ◽  
Resistance Mechanisms ◽  
Blood Cultures ◽  
Immunochromatographic Assay ◽  
Economic Costs ◽  
Major Threat ◽  
Improving Patient Care

Antibiotic resistance poses a major threat to health and incurs high economic costs worldwide. Rapid detection of resistance mechanisms can contribute to improving patient care and preventing the dissemination of antimicrobial resistance.

Rapid detection of positive blood cultures.

1983 ◽  
Vol 18 (3) ◽  
pp. 716-718 ◽  
Author(s):  
B L Hawkins ◽  
E M Peterson ◽  
L M de la Maza
Keyword(s):  
Rapid Detection ◽  
Blood Cultures

scholarly journals Systematic Comparison of Four Methods for Detection of Carbapenemase-Producing Enterobacterales Directly from Blood Cultures

2019 ◽  
Vol 57 (11) ◽  
Author(s):  
Maria Meier ◽  
Axel Hamprecht
Keyword(s):  
Rapid Detection ◽  
Clinical Microbiology ◽  
Bloodstream Infections ◽  
Blood Cultures ◽  
Patient Treatment ◽  
Clinical Microbiology Laboratory ◽  
Microbiology Laboratory ◽  
Study Protocols ◽  
Sensitivity Specificity ◽  
Good Detection

ABSTRACT Early identification of infections caused by carbapenemase-producing Enterobacterales (CPE) can help to optimize patient treatment and improve outcome. In this study, protocols for rapid detection of carbapenemase production directly from positive blood cultures were developed applying a concentration and hemolysis step before a test for carbapenemase production was performed. Four different methods (three modified colorimetric assays [β-Carba, bcCarba NP, and NeoRapid Carb] and a variation of the carbapenem inactivation method [CIM] test with blood cultures [bcCIM]) were assessed on blood cultures spiked with 185 different molecularly characterized Enterobacterales isolates. The challenge collection included 81 carbapenemase-negative isolates and 104 CPEs (OXA-48 [n = 25], NDM [n = 20], KPC [n = 18], VIM [n = 25], GIM [n = 5], OXA-48-like [n = 9], and OXA-48-like plus NDM [n = 2]). The sensitivity/specificity was 99.0%/95.1% for bcCarba NP, 99.0%/91.4% for NeoRapid Carb, 100%/95.1% for β-Carba and 100%/100% for bcCIM. Weakly hydrolyzing carbapenemases (e.g., OXA-48-like) were also well detected by the assays. The time to result was 20 to 45 min for β-Carba, 2 to 3 h for bcCarba NP, 2.5 to 2 h for NeoRapid Carb, and 18 to 24 h for bcCIM. In conclusion, all assays demonstrated good detection of CPE. The protocols can be easily implemented in any clinical microbiology laboratory and could help to optimize therapy early in bloodstream infections by CPE.

scholarly journals 2175. Rapid Detection of Carbapenemase Producing Organisms Directly from Blood Cultures Positive for Gram-Negative Bacilli

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S738-S738
Author(s):  
William Stokes ◽  
Johann Pitout ◽  
Lorraine Campbell ◽  
Deirdre Church ◽  
Dan Gregson
Keyword(s):  
Predictive Value ◽  
Rapid Detection ◽  
Blood Cultures ◽  
Gram Negative ◽  
Carbapenem Resistant ◽  
Direct Testing ◽  
Appropriate Treatment ◽  
Risk Patients ◽  
Health Zone ◽  
Sensitivity Specificity

Abstract Background The rapid detection of carbapenemase-producing organisms (CPOs) directly from blood cultures (BC) positive for Gram-negative bacilli (GNB) may accelerate the appropriate treatment of at-risk patients. Our objective was to evaluate the performance of two commercial assays in the rapid detection of CPOs directly from BC positive for GNB. Methods BC positive for GNB, taken from patients within the Calgary Health Zone over a 3 month period, were tested for the presence of CPOs with βCARBA® and NG-Test® CARBA 5. A subset of sterile BC samples was seeded with multi-drug-resistant (MDR) GNB. BC were incubated using the Bact-Alert™ system. Positive BC from clinical and seeded samples was tested directly with βCARBA and CARBA 5 from BC pellets processed for direct testing using an ammonium chloride lysis and wash method. Sensitivity, specificity, negative predictive value (NPV) and positive predictive value (PPV) were calculated with 95% confidence intervals for binomial proportions. Results 65 samples were tested (30 clinical, 35 seeded). Seeded samples included 1 GES, 4 IMP, 6 KPC, 1 co-producing KPC and NDM, 9 OXA, 4 VIM, 5 NDM, and 5 non-CPO carbapenem-resistant organisms. βCARBA had a sensitivity, specificity, NPV and PPV of 100% (88.4% - 100%), 65.7% (47.8–80.9%), 100%, and 71.4% (61.3%–79.8%), respectively. CARBA 5 had a sensitivity, specificity, NPV and PPV of 90.0% (73.5%–97.9%), 100% (90.0%–100%), 92.1% (80.0%–97.2%), and 100%. When excluding GES, which is known not to be detected by CARBA 5, sensitivity and NPV increased to 93.1% (77.2%–99.2%) and 93.1% (78.0%–98.1%), respectively. False negatives for βCARBA occurred with 1 VIM-1 and IMP-14. Conclusion This study demonstrates that the detection of CPOs directly from positive BC can be accurately achieved. βCARBA had excellent sensitivity but suffered from poor specificity. CARBA 5 had good sensitivity and specificity but is unable to detect certain CPOs. Testing positive BC directly using βCARBA and/or CARBA 5 may be useful in rapidly detecting CPOs. Results of direct testing from the CARBA5 assay would quickly identify patients amenable to treatment with avibactam combination compounds. Disclosures All authors: No reported disclosures.

scholarly journals Rapid detection of carbapenemase-producing Enterobacteriaceae from blood cultures

2014 ◽  
Vol 20 (4) ◽  
pp. 340-344 ◽  
Author(s):  
L. Dortet ◽  
L. Bréchard ◽  
L. Poirel ◽  
P. Nordmann
Keyword(s):  
Rapid Detection ◽  
Blood Cultures

scholarly journals Rapid detection of extended-spectrum β-lactamase-producing Gram-negative bacilli in blood cultures

2007 ◽  
Vol 60 (3) ◽  
pp. 652-654 ◽  
Author(s):  
Sarjana Jain ◽  
Jenny Andrews ◽  
Adam Fraise ◽  
Nigel Brenwald
Keyword(s):  
Rapid Detection ◽  
Blood Cultures ◽  
Gram Negative ◽  
Extended Spectrum

scholarly journals Rapid Detection of Salmonella enterica Serovar Choleraesuis in Blood Cultures by a Dot Blot Enzyme-Linked Immunosorbent Assay

2000 ◽  
Vol 7 (6) ◽  
pp. 977-979 ◽  
Author(s):  
Kritsana Janyapoon ◽  
Sunee Korbsrisate ◽  
Hatairat Thamapa ◽  
Sittichai Thongmin ◽  
Suwattana Kanjanahareutai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Salmonella Enterica ◽  
Rapid Detection ◽  
Direct Detection ◽  
Blood Cultures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Biochemical Method ◽  
Dot Blot

ABSTRACT A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella entericaserovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Choleraesuis were positive on testing by the ELISA. In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%.

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